Antisense oligonucleotide-carbon nanotube medicament vector

A technology of oligonucleotides and carbon nanotubes, applied in the field of drug carriers and their preparation, can solve the problems of low transfection rate and ineffective effect of tumor cells, and achieve high transfection rate, obvious effect and simple preparation method Effect

Inactive Publication Date: 2007-08-15
SHANGHAI NORMAL UNIVERSITY
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The technical problem to be solved by the present invention is to provide an antisense oligonucleotide-carbon nanotube drug carrier, which can effectively improve the loading and targeting of a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antisense oligonucleotide-carbon nanotube medicament vector
  • Antisense oligonucleotide-carbon nanotube medicament vector
  • Antisense oligonucleotide-carbon nanotube medicament vector

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1

[0045] 1. Preparation of acidified MWNTs

[0046] Put MWNTs in the mixed acid (H 2 SO 4 With HNO 3 The volume ratio of 3:1), ultrasonic for 16 hours. Filter the obtained MWNTs suspension with a 100nm microporous membrane, and rinse with twice distilled water until it is neutral. Re-disperse it in double distilled water, and remove the large particles of MWNTs by centrifugal filtration (7000rpm, 5min) several times, and obtain MWNTs with a length of 100-300nm and modified with -COOH on the tube wall (infrared spectrum Figure 2 , TEM image 3).

[0047] 2. Preparation of PEI-MWNTs water phase system

[0048] Preparation principle: Use the electrostatic force between groups.

[0049] Mix 0.5 mg MWNTs with 5 ml 1% polyethyleneimine (PEI). Ultrasound for 15 minutes, filter the resulting mixture with a 200nm microporous membrane, remove excess unreacted PEI, and disperse it in PBS with pH=7.2 to obtain a stable PEI-MWNTs aqueous phase system. Sterilize for use.

[0050...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

This invention relates to an antisense oligonucleotide-carbon nanotubes medicine carrier which comprises carbon nanotube casing and antisense oligonucleotide. The preparation method comprises oxidizing multi-wall carbon nanotube with strong acid to carry carboxyl on the shell of pipe, cutting to 50-200nm with diameter of 10-50nm with ultrasound, adding poly-cationization bichloride to embellish on shell of carbon nanotube, and assembling antisense oligonucleotide to carbon nanotube. The preparation method is simple and rapid. The medicine carrier has high transfection efficiency, high target effect, and good effect in inhibiting tumor cell. Targeted bimolecular or medicine with carbon nanotube as carrier material aim at different biological system can be applied for treating tumor or genetic diseases.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a drug carrier composed of oligonucleotide and nanometer material and its preparation method and application. Background technique [0002] Selecting a suitable target gene as an attack point and a suitable carrier to increase the expression of drugs in vivo are two major issues encountered in the study of tumor therapy. However, the progress made in the molecular biological characteristics of tumors and biocompatible chemical materials in the past 20 years has laid a good foundation for the selection of target genes. [0003] Human telomeres are repetitive DNA sequences (TTAGGG) located at the ends of chromosomes, which play an important role in maintaining chromosomal stability. The change and stability of telomeres are closely related to cell aging and canceration. Telomerase is a special ribonucleoprotein polymerase, which can use its own RNA as a template to sy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K47/26A61K47/04A61K48/00A61P35/00
Inventor 贾能勤连琼
Owner SHANGHAI NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap