14results about How to "Has clinical application value" patented technology

The application discloses an inhibitor for inhibiting KRAS phase separation to play an anti-tumor role and application, and relates to the fields of tumor molecular biology and targeted therapy.The application aims at solving the problems of limited effect, strong drug resistance and limited application range of KRAS tumor treatment methods, and the inhibitor is a statin.The KRAS protein can form a liquid condensate in cells, and the application discloses the mechanism of the KRAS protein in promoting KRAS protein processing, transportation and downstream signal activation, and further provides a technical scheme for inhibiting tumor growth and enhancing the therapeutic effect of a KRAS inhibitor by inhibiting the formation of KRAS condensates, so as to provide a new target and intervention approach for tumor treatment.

The application belongs to the technical field of biology, and discloses an antibody or antigen binding fragment thereof against monkeypox virus E8 and application thereof. The application provides a monoclonal antibody 2F6, 9G4, 9H1 or 10E11 against monkeypox virus E8, which can specifically recognize and target orthopoxviruses such as monkeypox and vaccinia, and shows high virus neutralization capacity. Moreover, the monoclonal antibody has clinical application value for preventing and treating orthopoxvirus infection, and can be used for detecting orthopoxviruses, diagnosing diseases related to orthopoxvirus infection, and preventing and / or treating orthopoxvirus infection and / or diseases related to orthopoxvirus infection.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.

The invention provides a Parkinson's disease rapid RT-QuIC diagnostic kit based on A30P mutant alpha-synuclein, and belongs to the technical field of detection kits. The specific A30P mutant protein is used as a reaction substrate, and rapid and high-sensitivity amplification detection can be carried out on trace misfolded alpha-synuclein in a skin sample through an optimized RT-QuIC system. The kit has the characteristics of short detection period and convenience in operation, provides a new tool for molecular diagnosis of Parkinson's disease, is suitable for definite diagnosis of clinical PD suspected cases, and can also be used for screening high-risk groups. The kit has the advantages of mature preparation process, easily available raw materials, easiness in industrial production and large-scale popularization, and important clinical application value.

This invention relates to the fields of biomedical engineering and temperature control technology, and discloses a method and system for predicting the three-dimensional temperature field during the cooling and rewarming process of organ cryopreservation. The method includes: constructing and preprocessing a simulation dataset and a real experimental dataset covering the cooling and rewarming process; constructing a transient temperature query network, fusing global physical field features, multi-scale local features, and spatial location information; training the model using a fusion physical constraint loss function containing data fitting loss and spatial smoothing loss; and inputting the thermophysical parameters of the organ to be predicted and sparse temperature observation data to achieve accurate prediction of transient temperature at any spatial location. This invention effectively alleviates the prediction bias caused by the difference between simulation and real distributions, improves the model's generalization performance and the physical rationality of the prediction results, and can be directly applied to fine-grained temperature control systems for organ preservation, providing reliable support for organ function protection and improved transplant success rates, thus possessing significant clinical application value.

This invention relates to a fusion antigen mRNA molecule, which encodes a protein comprising at least two of the following: hepatitis B surface antigen protein sHBsAg, hepatitis B core antigen protein Core, hepatitis B X antigen protein HBxAg, and hepatitis B preS1 antigen protein PreS1, preferably comprising hepatitis B surface antigen protein sHBsAg, hepatitis B core antigen protein, and hepatitis B PreS1 antigen protein, denoted as sHBsAg-Core-PreS1, and its amino acid sequence is shown in SEQ ID No. 17; the above-mentioned fusion antigen mRNA molecule can be prepared into an mRNA-LNP vaccine composition and used to treat chronic hepatitis B virus infection. The preferred sHBsAg-Core-PreS1 mRNA-LNP of this invention can normally express and present each encoded target antigen in vivo, and achieve cross-presentation, cross-activation and enhanced expression, inducing target antigen-specific humoral and cellular immune responses. It can also effectively overcome immune tolerance in a mouse model of chronic hepatitis B, and has excellent antiviral and immunotherapeutic effects. Compared with existing related therapeutic HBV mRNA-LNP vaccines, it effectively improves the immune tolerance breakthrough threshold, providing a candidate vaccine and a new immunotherapy strategy for the immunotherapy of patients with chronic HBV infection.

The invention discloses a multi-modal image fusion method and device based on a graph neural network and a storage medium in the technical field of medical image processing and artificial intelligence. The method comprises the following steps: acquiring a multi-modal image; performing depth feature extraction on the multi-modal image to obtain a high-dimensional feature map of each modal; dividing the high-dimensional feature graph of each modal into a plurality of graph nodes, calculating the similarity between each graph node and an adjacent node thereof, and constructing an adjacent matrix of a multi-modal graph structure according to the similarity; taking an adjacent matrix as graph topology guidance, capturing a global association relationship and a long-distance dependency relationship of node features in graph nodes, and performing global aggregation according to an attention pooling graph reading function to generate global features; and inputting the global features into the reconstruction network to generate a final fusion image. The technical problems that in the prior art, the modeling capacity for irregular data is limited, and complex spatial association and semantic dependence between multi-modal images are difficult to effectively capture can be solved.

The invention discloses an auxiliary supporting type nasal oxygen cannula suitable for a patient suffering from bucktooth and incisor tooth loss, and relates to the technical field of nasal oxygen canals, the auxiliary supporting type nasal oxygen cannula comprises a nasal oxygen cannula main body and an oral cavity supporting assembly, and the oral cavity supporting assembly is connected with the nasal oxygen cannula main body through a buckle type connecting assembly; the oral cavity supporting assembly comprises an adjustable occlusal pad and a plurality of anti-slipping buckles evenly arranged on the adjustable occlusal pad, the whole adjustable occlusal pad is of an arc-sheet-shaped structure, and the radian of the arc-sheet-shaped occlusal pad can be adjusted. The anti-slip buckle is used for hooking a gingival groove and abutting against the hard palate so as to fix the adjustable occlusal pad. According to the invention, the conversion from face fixation to stable support in the oral cavity is realized through structural innovation, a safe, comfortable and stable oxygen supply solution is provided for patients with abnormal tooth structures, the treatment compliance and life quality are remarkably improved, and the device has important clinical application value.

This research relates to the biomedical field and describes a thermosensitive hydrogel loaded with ginger extracellular vesicles and tannic acid, its preparation method, and its application in the preparation of anti-infective drugs. The process includes extracting ginger-derived extracellular vesicles; loading tannic acid onto these vesicles to obtain TA@GDEVs; preparing SP hydrogels; and slowly adding TA@GDEVs to the SP hydrogel to form a GTSP hydrogel system. GDEVs exhibit anti-inflammatory and pro-repair activities; TA provides broad-spectrum antibacterial, anti-inflammatory, and antioxidant functions; the SP hydrogel endows the thermosensitive hydrogel composite system with injectable and in-situ gelation properties; the SP hydrogel not only provides in-situ gelation and a protective barrier for GDEVs and TA but also enables controlled release of the loaded material; through synergistic effects, the three components enable the thermosensitive hydrogel composite system to possess responsiveness to the infection microenvironment, enhanced antibacterial efficacy, immune remodeling, and tissue repair functions, demonstrating its potential as a multifunctional anti-infective wound repair material.

The invention discloses a keratoconus element learning diagnosis method under an unbalanced small sample condition. The method comprises two parts of model training and auxiliary reasoning. According to the model training part, firstly, an acquired corneal topographic map is preprocessed and labeled, and then a meta-learning task ensuring complete categories is constructed by adopting a balance task sampler. And a pre-trained Swin Transform is used as a feature extractor to acquire the multi-scale depth features of the image. A plurality of prototypes are generated for early keratoconus categories with high diagnosis difficulty by using K-means clustering, and other categories are uniformly grouped. Classification is completed by calculating the distance between a query sample and a prototype and carrying out probability aggregation, and meta-learning optimization is carried out by adopting a joint loss function combining enhanced focus loss and contrast learning loss. And the auxiliary reasoning part is used for extracting input image features by using the trained model, comparing the input image features with the learned prototype, and outputting a diagnosis category and confidence. According to the method, the auxiliary diagnosis accuracy and generalization ability of the keratoconus are remarkably improved.

The invention relates to a dendrimer-based polypeptide nano vaccine as well as a preparation method and application thereof. The polypeptide nano vaccine is obtained by loading MnO2 nano particles on a functionalized dendrimer and wrapping a polypeptide antigen. After the polypeptide nano vaccine disclosed by the invention is injected into a mouse body through peritumoral injection, MnO2 plays a chemical kinetic treatment role through a Fenton-like reaction, and tumor cells are induced to generate immunogenic cell death, so that dendritic cell maturation is promoted; meanwhile, the polypeptide nano vaccine can activate an immune system and initiate potent immune response by activating a cGAS-STING pathway and being cooperated and combined with presentation of a polypeptide antigen OVA257-264, a new chemical kinetics / immune combined treatment strategy is provided, and the polypeptide nano vaccine has a remarkable inhibition effect on tumor growth and recurrence and has potential application value in the field of biological medicine.

This invention discloses a method and system for intelligent identification and classification of breast lesions based on multimodal fusion, belonging to the field of image processing technology. This invention utilizes deformable registration technology based on multi-scale depth feature matching to accurately align local structures of lesions in ultrasound and X-ray images. Furthermore, through a cross-attention mechanism and threshold screening-based cross-modal feature selective fusion strategy, it can automatically identify and enhance highly correlated and discriminative feature pairs from different modalities, while suppressing redundant or contradictory interference information, thereby generating a more discriminative fused feature representation. This invention effectively overcomes the problems of inaccurate registration and inefficient fusion in existing technologies, significantly improving the accuracy and robustness of benign and malignant breast lesion classification. It provides clinicians with a more accurate and reliable multimodal image-based intelligent auxiliary diagnostic tool, possessing significant clinical application value.

The present application relates to the field of antibodies, and in particular, the present application provides a high affinity PADI2 antibody and a preparation method thereof. Through antibody screening experiments, eight high affinity PADI2 antibodies are screened, the antibodies can specifically bind to PADI2 protein, and have high affinity. Experimental results show that the antibodies of the present application exhibit excellent binding capacity in ELISA, Western blot and cell experiments, and can be used for detection, diagnosis and treatment of PADI2 related diseases.

The utility model discloses a bladder irrigation device, which belongs to the technical field of medical equipment, and comprises a base, the upper surface of the base is fixedly connected with a support rod, the upper end of the surface of the support rod is fixedly connected with two upper mounting plates, a first threaded screw rod is rotatably connected between the two upper mounting plates through a bearing, and a second threaded screw rod is rotatably connected between the two upper mounting plates through a bearing. The surface of the first threaded screw rod is sleeved with a flushing assembly in a threaded mode, two lower mounting plates are fixedly connected to the lower end of the surface of the supporting rod, a second threaded screw rod is rotationally connected between the two lower mounting plates through a bearing, and the surface of the second threaded screw rod is sleeved with a collecting assembly in a threaded mode. By arranging the flushing assembly, the height of the infusion bottle is adjusted, the operation convenience and flexibility are improved, the flushing effect is improved, by arranging the collecting assembly, the height of the waste bin is adjusted, the waste liquid collecting requirements of patients with different body positions are better met, and the waste liquid collecting efficiency is improved.