Applications of blood serum IL-6 for preparing diagnose kit for primary hepatocellular carcinoma

A diagnostic kit and technology for hepatocellular carcinoma, applied in the field of cancer diagnosis, can solve the problem of limited sensitivity of AFP, and achieve the effects of easy wide-scale popularization and application, simple operation, and broad market prospect.

Inactive Publication Date: 2009-03-25
SUN YAT SEN UNIV
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Problems solved by technology

However, the sensitivity of AFP is limited; especially in recent years, AFP-negative HCC patients have an increasing trend, so it is urgent to find early tumor markers other than AFP

Method used

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  • Applications of blood serum IL-6 for preparing diagnose kit for primary hepatocellular carcinoma
  • Applications of blood serum IL-6 for preparing diagnose kit for primary hepatocellular carcinoma
  • Applications of blood serum IL-6 for preparing diagnose kit for primary hepatocellular carcinoma

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Experimental program
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Embodiment Construction

[0009] Material:

[0010] Serum samples were taken, including 30 serum samples from normal physical examination people who were not infected with HBV, HCV, HIV, and tumors by clinical laboratory tests; 50 serum samples from HBV carriers, including 22 patients with major three yang and 28 patients with minor three yang ; 84 serum samples from patients with primary hepatocellular carcinoma, including 39 small liver cancers (diameter10cm). Moreover, among all the tested HCC samples, AFP≤25μg / L accounted for 33 samples, and AFP>25μg / L accounted for 51 samples.

[0011] The kit contains: 1. Human IL-6 recombinant monoclonal antibody coated on a solid-phase 96-well microtiter plate; 2. The enzyme conjugate working solution is human IL-6 labeled with horseradish peroxidase (HRP) -6 recombinant monoclonal antibody reaction solution; 3. The positive control is human IL-6 standard protein, and the negative control is standard negative serum; 4. Chromogenic solution ABTS substrate: ABTS...

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Abstract

The invention relates to a cancer diagnosis technology, in particular to application of serum IL-6 in the preparation of a primary hepatocellular carcinoma diagnostic reagent kit. The reagent kit can quickly and accurately detect the IL-6, thereby performing quick assistant diagnosis to primary hepatocellular carcinoma at each stage (particularly at early stage) and having important clinical application value. The invention comprises the establishment of an enzyme-linked immune diagnosis system based on a double antibody sandwich principle. The serum does not need to be diluted, so the sensitivity is greatly improved. The application has the advantages that the detection reagent kit has simple operation, steady reagent, good repetitiveness, strong specificity, and high sensitivity, is easy to promote and apply within large range, and has broad market prospect.

Description

[technical field] [0001] The invention relates to a cancer diagnosis technology, in particular to the preparation of a primary hepatocellular carcinoma diagnosis kit. [Background technique] [0002] Liver cancer is one of the most common malignant tumors in humans, and its incidence rate ranks fifth in the world, and it ranks third in tumor mortality. About 500,000 new cases are added every year, and the incidence rate after age correction is 5.5-14.9 / 100,000 people. Hepatocellular carcinoma (HCC) is the most important type of primary liver malignancy, and its high-incidence areas are mainly concentrated in East Asia, Southeast Asia, Central and West Africa and other regions. Since 1990, HCC has been the second highest cause of cancer death in China. Hepatitis B virus, hepatitis C virus infection, aflatoxin B1 intake and alcohol abuse are considered to be the main risk factors. Hepatocellular carcinoma is also one of the common malignant tumors in South China. Finding earl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 孔祥复谢丹朱萧陈敏山李东培王毅廖奕佶童铸廷李奎林李家宓
Owner SUN YAT SEN UNIV
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