35 results about "Erythrocyte sedimentation" patented technology

An apparatus and method for rapid determination of erythrocyte sedimentation rates for a blood specimen (29) which can be linearly transposed to Westergren sedimentation rates. The method includes the steps of inducing accelerated rouleaux formation in the specimen (29) in an amount sufficient to begin settling at substantially the decantation rate for the specimen. In one embodiment a structure (27) which produces a very thin cross-sectional region (37) of the specimen (29) inside the lumen (23) of a specimen container (21) is provided to accelerate rouleaux formation. In an alternative embodiment (120), accelerated rouleaux formation is accomplished using a centrifuge (122). A third embodiment employs a movable rod (223) mounted inside the specimen tube (221) to induce accelerated rouleaux formation. All embodiments of the process next employ gravity settling the specimen in a near horizontal oriented container (21, 121, 221). Thereafter, the amount of settling occurring is determined. A sealed specimen container (21, 121, 221) which permits thorough mixing of blood in a very small diameter container for use in performing the method also is provided.

A method for calibrating machines suitable to effect an analysis of a blood sample by means of measuring the erythrocyte sedimentation rate (ESR) and / or aggregation of the red corpuscles, wherein said measurement is effected by exploiting the optical density kinetics obtained from the measurement of the variation in the optical density of the blood sample in an interval of time, comprises a measuring step in which, by means of the same machine with which the measurement of the optical density is effected on said blood sample, a measurement is effected of the optical density of two latexes, or turbidimetric samples. Each of the two latexes has a known optical density, reproducible, measurable and different from each other. The method also comprises a comparison step, in which the difference is calculated between the values of optical density of the latexes as obtained from the measurement performed by the machine and the known values of optical density, to allow to determine at least one correction factor usable to calibrate said machine.

The invention discloses a blood detection micro-fluidic chip. The blood detection micro-fluidic chip comprises a substrate and a cover plate; and the cover plate and the substrate are in sealing fit to form a chip body; the chip body is provided with a plurality of separation and detection units, and the plurality of separation and detection units are radially distributed by taking the circle center of the chip body as an original point; each separation and detection unit comprises a whole blood sampling pool, a plasma separation pool, an erythrocyte sedimentation pool, a plasma storage pool, a quantification pool, a front primary waste liquid pool, a front secondary waste liquid pool, a rear primary waste liquid pool, a detection reagent sampling pool, a detection pool, a whole blood sample injection hole, a whole blood sample injection pool vent hole, a plasma storage pool vent hole, a rear primary waste liquid pool vent hole and a detection reagent sample injection hole, wherein the whole blood sampling pool, the plasma separation pool, the erythrocyte sedimentation pool, the plasma storage pool, the quantification pool, the front primary waste liquid pool, the front secondary waste liquid pool, the rear primary waste liquid pool, the detection reagent sampling pool and the detection pool are arranged on the substrate, and the whole blood sample injection hole, the whole blood sample injection pool vent hole, the plasma storage pool vent hole, the rear primary waste liquid pool vent hole and the detection reagent sample injection hole are formed in the cover plate. The blood detection micro-fluidic chip has separation and detection functions at the same time, can achieve plasma / serum separation, reagent injection, reaction and detection integration; and the problem that blood coagulation measurement and detection cannot meet the ever-increasing instant detection requirement is solved.

An hemoglobin (Hb)-Dextran (Dx) conjugate having a molecular weight between 50 kd and 500 kD provides a blood substitute that results in acceptable erythrocyte sedimentation rate (ESR) and excretion rate (EXC) values. DxHb conjugates of the invention can be used for a variety of purposes as an alternative to blood.

The invention discloses a rapid erythrocyte sedimentation rate measuring method and a measuring device thereof. The measuring method comprises the steps that the geometric size of an erythrocyte sedimentation rate tube is modified, a photovoltaic detecting sensor is used for detecting the height value change of the sedimentation surface of erythrocyte in the modified erythrocyte sedimentation ratetube in the erythrocyte sedimentation process, an erythrocyte sedimentation rate Westergren value is obtained by a modern signal processing method and a regression algorithm, the measuring time of the rapid measuring method is half the time of a traditional Westergren method, and the correlation of the rapid measuring method with the Westergren method achieves 99%. The measuring device comprisesa microprocessor 01, a motor driving module 02, a motor execution module 03, a mechanical motion module 04 and a photovoltaic module 05. A rapid erythrocyte sedimentation rate analyzing meter is established by the rapid erythrocyte sedimentation rate measuring method and the measuring device thereof, a rapid erythrocyte sedimentation, rapid painting of an erythrocyte sedimentation rate value and erythrocyte sedimentation rate process curve can be realized, erythrocyte sedimentation rate sample measuring of a large batch can be automatically carried out, and the practical value is high.

A method for calibrating machines suitable to effect an analysis of a blood sample by measuring the erythrocyte sedimentation rate (ESR) and / or aggregation of the red corpuscles, wherein the measurement is effected by exploiting the optical density kinetics obtained from the measurement of the variation in the optical density of the blood sample in an interval of time, to include measuring in which, by the same machine with which the measurement of the optical density is effected on the blood sample, a measurement is effected of the optical density of two latexes, or turbidimetric samples. Each of the two latexes has a known optical density that is reproducible, measurable and different from each other. The method also calibrates in which the difference is calculated between the values of optical density of the latexes as obtained from the measurement performed by the machine and the known values of optical density, to determine at least one correction factor usable to calibrate machine.

The invention discloses a Widmannstatten method full-automatic erythrocyte sedimentation rate dynamic analyzer and a detection method, and the analyzer mainly comprises a transmission device used for placing a blood sample to be detected, the transmission device comprises a rotating disc, and the rotating disc is provided with a plurality of hole sites used for placing the blood sample; the sample introduction device is used for sucking a blood sample into the Widmanni erythrocyte sedimentation glass tube, and the blood sample enters the Widmanni erythrocyte sedimentation glass tube through the sample introduction needle, the valve group and the silicone tube connected with the Widmanni erythrocyte sedimentation glass tube; the detection device is used for detecting the liquid level height of the blood sample in the Widmanni erythrocyte sedimentation glass tube; the cleaning device is used for cleaning and blow-drying the Widmannstan erythrocyte sedimentation glass tube after the test is finished. By adopting the analyzer and the detection method, the working efficiency can be improved, the reading is accurate, the operation is simple, the biological hazard is reduced, and the cleaning is convenient.

The invention discloses a plasma extraction device based on a microfluidic chip. The device comprises a microfluidic chip and a matched driving module. During use, whole blood is added into the microfluidic chip to leave the whole blood to stand for a while, almost red blood cells are settled in the bottom of a reaction chamber, and a small amount of red blood cells and plasma are located in the top layer of the reaction chamber; by means of an external mechanical action force, compression deformation on height of the reaction chamber of the microfluidic chip happens by means of the driving module, and the volume becomes small, so that the plasma and part of red blood cells are discharged out of the reaction chamber through a whole blood filter membrane, the red blood cells are blocked by micropores in the filter membrane while the plasma passes through the filter membrane, and is exported through the micropores of a transfer structural sheet, so that the plasma in the whole blood is extracted. The plasma extraction device based on the microfluidic chip is simple to operate, does not need complicated driving devices, and has an ideal plasma extraction efficiency. The plasma extraction device can be combined with other microfluidic detection modules to separate the plasma in a whole blood sample and detect a landmark of a disease.

The invention discloses a chrysanthemum total flavone granule, which is made of the following raw materials in the weight ratio: sucrose powder: dextrin: dry extract powder of the total flavonoids of chrysanthemum = 0.5-1.5: 1.5-2.5: 0.5-1.5 . The present invention prepares chrysanthemum total flavonoids into granules. The granules have good formability, properties, particle size, water content, solubility, etc. all meet the requirements, and the quality is controllable and the stability is good. The color changes, the smell is lost, the content of active ingredients decreases, and the color of the item in the soaking process does not turn green. Chuju total flavonoids granules can significantly prolong the coagulation time of rats with cold coagulation and blood stasis syndrome, reduce the length of thrombus in vitro and dry and wet weight, reduce hematocrit, slow down erythrocyte sedimentation rate, and increase serum NO content. The product is easy to drink and carry, easy to store, has no toxic and side effects, and has the characteristics of preventing, treating and improving abnormal blood rheology in rats with blood stasis.

The invention discloses a preparation method of hydroxyapatite by using red blood cells as bioreactor, which belongs to the technical field of synthesis of inorganic nano materials. The technical proposal of the invention comprises the following main points that the upper plasma is removed from animal blood or human blood by centrifugation and the remainder is washed with physiological saline several times to obtain erythrocyte sedimentation, the erythrocyte sedimentation is added to the physiological saline containing soluble barium salt and the reaction is carried out under the condition of ice-water bath, then the mixture obtained by the reaction is centrifuged and washed after the reaction, the erythrocyte sedimentation obtained by the centrifugation is collected and resuspended in the PBS buffer solution(PH=9) to carry out the reaction, and functional red blood cells containing hydroxyapatite are obtained by centrifugation after the reaction, then the hydroxyapatite nanoparticles with an average particle size of 5.6 nm are prepared by the crush, separation and purification of the functional red blood cells. The invention has a series of advantages of green environmental protection, simple operation, low cost and good biocompatibility.

The invention discloses sample density separation liquid which comprises the following components in percentage by mass: 5-10% of glycerin, 3-5% of ethanol, 3-5% of sodium chloride, 79.8-88.9% of double distilled water and 0.1-0.2% of tea saponin. By adopting the sample density separation liquid, the cells of diagnostic value can naturally sedimentated more quickly while the erythrocyte sedimentation interference is reduced, thus a microscopic examination slice has clearer microscopic examination view and good cell dyeing contrast, and the sample density separation liquid can be widely applied to the exfoliative cytology specimens of gynecology, non-gynecology and the like and to a manual, semi-automatic or full-automatic liquid-based cell slide preparation process.

The present invention relates to the use of a combination of: (i)a macromolecular erythrocyte sedimentation enhancer, and (ii) dimethyl sulphoxide (DMSO), dimethylglycine (DMG) and / or valine; to recover non-erythrocyte blood cells from a blood cell-containing sample and / or to prime non-erythrocyte blood cells to protect their integrity in subsequent cryopreservation step(s).