9 results about "Erythrocyte aggregation" patented technology

The invention discloses a diluent for whole blood lateral immunochromatography detection as well as a preparation method and a detection method thereof, and belongs to the technical field of immunochromatography detection. The diluent solves the technical problems of unsmooth chromatography, background interference, poor stability of detection results and the like caused by red blood cells during direct detection of whole blood samples. The diluent comprises a buffer system, an osmotic pressure regulator glycine, a nonionic surfactant polysorbate, an erythrocyte aggregation inducer polyvinylpyrrolidone, a dynamic viscosity regulator trehalose, a blocking protein, a stabilizer combination L-methionine and L-histidine, and a composite formula of water. Red blood cells are effectively induced to gather and intercept on the sample pad through the synergistic effect of all the components, and meanwhile the stability of the chromatography process and the activity of the detection reagent are maintained. The diluent is mainly used for lateral immunochromatography quantitative detection of markers such as pro-enkephalin, C-reactive protein, cardiac troponin I or N-terminal pro-brain natriuretic peptide in a whole blood sample.

The invention relates to a blood rheological parameter online monitoring and thrombus risk assessment method, which comprises the following steps: introducing a blood sample into a microfluidic channel with a periodic geometric mutation structure, applying unsteady shear disturbance through a piezoelectric oscillation device, inducing blood to enter a non-equilibrium rheological state, and exciting a microcosmic coagulation tendency; the red blood cell aggregation behavior, the cell layer slippage phenomenon and the plasma shearing stability are recognized through the photo-thermal interference technology, and the rheological structure layer differentiation characteristics in blood are obtained; the blood subjected to shear disturbance is guided to a micro-channel high-pressure area provided with a flow-limiting threshold structure, and pressure mutation and microcoacervation nucleation generation behaviors induced by local stress disturbance are detected through a nanoscale piezoresistive sensor so as to identify sub-phase change response characteristics of the blood; and constructing a rheological instability index system on the basis of physical response signals obtained by shear disturbance, slippage and variable response, and when linkage abnormity of any two indexes is detected, judging that a high-risk event of thrombosis occurs and outputting a risk prompt signal.

According to the invention, blood group antigens suitable for allogenic blood transfusion detection are screened and combined, and blood group antibody selection, fluorescent antibody screening, automatic cell cleaning program control, cleaning fluid selection, erythrocyte aggregation resistance, allogenic erythrocyte determination and other multi-condition combination methods are adopted. The detection rate of the positive sample of the allogenic blood transfusion stimulant is further effectively improved, and the accuracy of allogenic blood transfusion detection is improved.

The invention provides application of CLCA1 as an intervention target spot of thrombus generation, and belongs to the field of biological medicine. It is found for the first time that CLCA1 can participate in thrombosis, and low expression of CLCA1 can reduce the blood flow velocity of blood vessels, promote erythrocyte aggregation in the blood vessels and up-regulate the expression level of thrombus-related genes Fga, f7 and PAI-1. The CLCA1, as an intervention target of thrombus occurrence, can be used for screening or preparing antithrombotic drugs, preparing products for evaluating thrombus occurrence risks and constructing animal thrombus models, and has a clinical application prospect.

The invention discloses a preparation method and application of hemostatic hydrogel based on phytolectin. Belongs to the technical field of materials. The preparation method comprises the following steps: (1) performing sulfhydrylation treatment on sodium alginate; (2) introducing a solution containing the phytolectin and calcium ions into the obtained product to obtain the thiolated sodium alginate-calcium ion hydrogel encapsulated with the phytolectin; the entrapment effect of the hemostatic hydrogel on the phytolectin is enhanced by disulfide bonds formed by crosslinking of internal sulfydryl in the hemostatic hydrogel, and potential biotoxicity caused by leakage of the phytolectin is avoided; according to the hemostatic hydrogel, rich sulfydryl groups on the surface of the hemostatic hydrogel can be tightly adhered to a wound part to efficiently adsorb erythrocytes, then phytolectin in the hydrogel induces erythrocyte aggregation and promotes formation of a fibrin network to enhance the stability of blood clots, and finally efficient wound hemostasis is achieved. In addition, the hemostatic hydrogel is good in biocompatibility, mild in gelation condition and simple to prepare, and has a good clinical application prospect.

A composition including modified ASFV outer-membrane protein antigen mutants (termed dominant negative toxoid antigens) that exhibit non-binding affinity to RBCs while inducing an antibody-mediated response capable of neutralizing unmodified proteins found on infectious outer-membrane-laden ASFV virions. A method for the treatment and / or prevention of ASFV by administering a dominant negative toxoid antigenic composition to animals, thereby averting RBC aggregation caused by the antigen and concurrently treating and / or preventing ASFV. An ASFV vaccine composition including dominant negative toxoid antigens. A composition including dominant negative toxoid antigens in conjunction together and in conjunction with antigens derived from capsid-based proteins, which collectively target both lysogenic and lytic viral replication cycles, thereby achieving optimal immune stimulatory protection. Methods and compositions allowing for differentiation of infected from vaccinated animals (DIVA).

The present application relates to a blood sedimentation measurement method, a sample analyzer and a sample analysis system. A sample to be measured is sucked from a test tube containing the sample to be measured after a mixing operation, at least a part of the sample to be measured is transported to a detection line and stopped in the detection line; the sample to be measured stopped in the detection line is irradiated with light, and information of transmitted light or information of scattered light scattered by the sample to be measured is obtained; a red blood cell aggregation parameter of the sample to be measured is obtained based on the information of transmitted light or the information of scattered light; the sample to be measured in the test tube is allowed to stand for a predetermined period of time from a predetermined time after the mixing operation, so as to obtain a red blood cell sedimentation parameter of the sample to be measured, and the predetermined period of time is not more than 20 minutes; and a red blood cell sedimentation rate of the sample to be measured is calculated based on the red blood cell aggregation parameter and the red blood cell sedimentation parameter. Thus, an accurate red blood cell sedimentation rate can be quickly obtained.

The invention relates to an erythrocyte sedimentation measuring method, a sample analyzer and a sample analysis system. Sucking the to-be-detected blood sample from the test tube filled with the to-be-detected blood sample subjected to the uniform mixing operation, conveying at least one part of the to-be-detected blood sample to the detection pipeline, and enabling the part of the to-be-detected blood sample to stop flowing in the detection pipeline; irradiating the to-be-detected blood sample which stops flowing in the detection pipeline with light, and acquiring information of transmitted light penetrating through the to-be-detected blood sample or information of scattered light scattered by the to-be-detected blood sample; acquiring erythrocyte aggregation parameters of the to-be-detected blood sample based on the information of the transmitted light or the information of the scattered light; allowing the to-be-detected blood sample in the test tube to stand for a predetermined time period from a predetermined moment after the uniform mixing operation so as to obtain erythrocyte sedimentation parameters of the to-be-detected blood sample, the predetermined time period being not more than 20 minutes; and calculating the erythrocyte sedimentation rate of the to-be-detected blood sample based on the erythrocyte aggregation parameter and the erythrocyte sedimentation parameter. Therefore, the accurate erythrocyte sedimentation rate can be quickly obtained.

This invention improves the detection rate and accuracy of allogeneic transfusion detection by screening and combining blood type antigens suitable for allogeneic transfusion detection, and by combining multiple conditions such as blood type antibody selection, fluorescent antibody screening, automated cell washing program control, washing solution selection, anti-erythrocyte aggregation, and allogeneic erythrocyte determination methods.