31 results about "Erythrocyte aggregation" patented technology

The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.

The invention provides a chitosan fiber-based efficient hemostatic material and a preparation method thereof. The preparation method comprises the following steps of spinning chitosan to obtain a chitosan fiber base material, and grafting carboxyl and catechol structures on the chitosan fiber base material at the same time. The hemostatic material can quickly absorb moisture in blood to swell, so that the hemostatic rate is remarkably improved. The carboxyl is introduced, so that the hemostatic material has super-strong imbibition capacity, platelets in blood of a bleeding part are quickly adhered and aggregated to form thrombus, hydroxyl on the catechol group can be complexed with iron ions in red blood cells, the red blood cells are aggregated to play a role in stabilizing blood clots, and finally, the effect of quickly stopping bleeding is achieved. The efficient hemostatic material provided by the invention is especially suitable for the condition of massive bleeding.

Provided herein are kit for providing a gamma sterilized aqueous dextran solution that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation while maintaining sufficient molecular weight to act as a red blood cell aggregate. Also provided are methods of use.

The invention discloses a citrus bioflavonoid active beverage and a making method thereof. By means of bioflavonoid extracted from biological raw materials through deep processing, the beverage is prepared from, by mass, 80% of water, 8% of fructo-oligose, 7% of polydextrose and 5% of bioflavonoid. The flavonoid functional beverage obtained through the method creates the environment protection benefits, the economic benefits and the social benefits; meanwhile, citrus bioflavonoid has effects of vitamin P samples and can influence the functions of blood vessel wall cells and blood platelets, the functions of white blood cells, the coagulation process, the hemorheology and the formation of thrombus, multi-methoxy compounds such as nobiletin, sinensetin and tangeritin in citrus bioflavonoid have important pharmacological effects, erythrocyte aggregation in human bodies can be reduced, and the blood cell sedimentation rate is decreased.

A process for the detection of antibodies in a test sample by carrying out the following steps:

• (a) preparing a suspension of erythrocytes with a test serum or plasma by mixing a test serum or plasma with erythrocytes;
• (b)incubating the suspension formed in step (a) at a temperature of from 37° C. to 45° C. for a period of time from sixty seconds to 10 minutes to bind any antibodies in the test serum or plasma to the surface of said erythrocytes
• (c)combining said suspension with an amount of a solution of a macromolecule which is effective for agglutination of said erythrocytes;
• (d) packing the resultant red cell agglutinates by centrifugation from the suspension of step (c); and,
• (f) determining the presence of anti-erythrocyte antibodies by observing if antibody-dependent erythrocyte aggregation has occurred.

The invention provides an erythrocyte aggregation detection method and a system thereof, which relate to the fields of biological and microbiological analysis detection. The method comprises the following steps: connecting an analysis module with an image information acquisition module and a control module for analyzing an obtained microscopic image; performing edge detection on erythrocyte in themicroscopic image, filling up an internal gas of the edge to form a complete erythrocyte shape image; performing a shrinkage analysis on an outer contour of the erythrocyte, and when the result is higher than a set value, determining the result as erythrocyte aggregation. calculating a ratio of an erythrocyte area with aggregation and an average area of the normal erythrocyte, and determining theerythrocyte aggregation degree according to the ratio. Compared with the prior art, the method employs a direct observation method and an image analysis method, the erythrocyte can be automatically identified, the area of an erythrocyte image and the area of the normal erythrocyte are compared, the erythrocyte aggregation degree is determined, the operation is convenient, the measurement efficiency is high, the identification precision is high, and the method can be used for quantitative analysis.

The invention discloses a chitosan hemostatic gel and a preparation method thereof. The chitosan hydrogel is glycidyl ether modified chitosan dissolved in water. For the deficiency that the blood coagulation rate of the common chitosan material is relatively slow, a hydrophobic chitosan derivative is prepared by introducing the alkane long chain in the invention; when in contact with blood, the hydrophobic group thereof can promote erythrocyte aggregation to realize rapid blood coagulation; the chitosan hemostatic gel in the invention is the gel dosage form, which is convenient to use and carry; and the preparation method adopted in the invention is simple and convenient in process and convenient to operate, and can be used for large-scale industrial production.

A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.

The invention discloses a method of analyzing a platelet aggregation function according to platelet size changes, and belongs to the field of clinical laboratory medicine. The method of analyzing the platelet aggregation function according to the platelet size changes measures the platelet size changes before after aggregation processing, and calculates the aggregation rate value of platelet according to the size changes of the platelet in a sample, and therefore the strength of the platelet aggregation function can be reflected. The method of analyzing the platelet aggregation function according to the platelet size changes also can be applied to erythrocyte aggregation rate analysis.

The invention relates to a medicine for curing cerebral thrombosis and a preparation method thereof. The medicine of the invention is prepared by raw medicines with portions according to the following weight ratio: 70-90 portions of Bupleurum chinense DC, 90-110 portions of RhizomaChuanxiong, 70-90 portions of root of red rooted salvia, 150-170 portions of radix astragali, 80-100 portions of earthworm, 50-70 portions of radix curcumae, 140-160 portions of core-removing hawthorn, 40-50 portions of radix notoginseng and 1.5-2.5 portions of borneol. The preparation method comprises that the radix notoginseng are ground into 120 meshes of fine powder, the borneol is ground into 120 meshes of fine powder, and the Bupleurum chinense DC, the RhizomaChuanxiong, the root of red rooted salvia, the radix astragali, the earthworm, the radix curcumae and the core-removing hawthorn are cut into chips which are decocted with 1000g water for three times and each time is decocted for one hour; the raw materials being decocted are extracted, filtered, compressed, dried and ground into 120 meshes of fine powder, in which the radix notoginseng fine powder and the borneol fine powder are added and mixed evenly, thus obtaining the medicine through split charging into 0.5g of capsules. The medicine of the invention can promote blood circulation by removing blood stasis, warm channels and expel cold, restore consciousness and resuscitate and resist platelet aggregation; moreover, the medicine of the invention also can replenish qi to secure the exterior, clear away heat and toxic material, nourish blood and tranquilize mind and resist thrombosis; furthermore, the medicine of the invention can relieve the depressed liver, cool blood and remove stagnation and reduce erythrocyte aggregation; the medicine further can relieve muscles and warm channels, promote salivation and activate yang, dispel wind and toxicity, relieve heart and restlessness and regenerate tissues.

The invention relates to a supported hemoglobin composition and a preparation method thereof. The preparation method comprises the following steps: (1) dissolving a carrier polyethylene glycol-poly(lactide-co-glycolide) into an organic solvent, mixing with a water solution of hemoglobin, and carrying out ultrasonic treatment to form primary emulsion; (2) adding a water solution of a surfactant to the primary emulsion obtained in the step (1), mixing and carrying out ultrasonic treatment to form a compound emulsion; and (3) removing the organic solvent, and centrifuging to obtain the supported hemoglobin composition. The supported hemoglobin composition is prepared by a double emulsion method; the method is simple and feasible; the prepared supported hemoglobin composition has oxygen carrying and releasing functions, has rheological characteristics similar to whole blood, is high in stability and good in blood compatibility, and does not cause erythrocyte aggregation; and the carrier material provided by the invention is prepared from abundant raw material, has the advantages of low cost, biocompatibility, biological affinity and no toxicity and is prone to be chemically modified.

The invention discloses a method for dissolving fat by using laser and a magnetic field. The method comprises the following steps: S101, cleaning a to-be-weight-reduced part of a to-be-weight-reducedperson; S102, soaking the to-be-weight-reduced part in hot water for 10-15 minutes; S103, using 20mW and 650nm low-intensity laser to continuously irradiate the to-be-weight-reduced part for 10 to 15minutes; S104, continuously irradiating the to-be-weight-reduced part for 20-30 minutes by adopting a plurality of groups of pulse emission sources and emitting extremely low frequency PEMF; and S105,taking warm-keeping measures on the to-be-weight-reduced part. The red blood cell aggregation degree can be reduced, the blood flow rate is increased, oxygen utilization of whole body blood is promoted, and the human body vitality is enhanced; free radicals accumulated in the body are adjusted, mobilized and decomposed, metabolism is accelerated, blood circulation is promoted, the free radicals in the body are removed, and then the laser fat-reducing effect can be greatly improved.

The invention discloses an ultrasonic erythrocyte aggregation degree detection, regulation and control system. The equipment comprises an ultrasonic equipment end and a computer processing end connected with the ultrasonic equipment end; the ultrasonic equipment end is used for transmitting an ultrasonic pulse signal to a target human body and collecting blood ultrasonic radio frequency data of the target human body; the computer processing end is used for detecting the erythrocyte aggregation degree of the target human body according to the blood ultrasonic radio frequency data and sending a regulation and control signal to the ultrasonic equipment end when the erythrocyte aggregation degree is greater than a set aggregation degree; and the ultrasonic equipment end is further used for sending ultrasonic regulation pulse waves to the target human body according to the regulation and control signal until the erythrocyte aggregation degree of the target human body reaches the set aggregation degree. The erythrocyte aggregation degree can be detected and regulated in real time, and the detection and regulation efficiency and accuracy are improved.

The invention provides a fat dissolving system and a fat dissolving method, and belongs to the technical field of medical cosmetology. The fat dissolving system comprises a laser module and a controller module and further comprises a pulse electromagnetic field module and a fusion handle. According to the system and the method, the laser module emits laser energy, active oxygen level of fat cellsis improved through laser heating effect, lipid peroxidation of the fat cells is caused, cell membranes of the fat cells are damaged and fused, and the fat cells are enabled to be liquefied. Bionic pulsed magnetic resonance is formed by the pulse electromagnetic field module, an extremely low-frequency PEMF (pulse electromagnetic field) is emitted, sodium and potassium ions and ATPase on surfacesof erythrocyte membranes are activated, cell membrane potential is repaired, the aggregation extent of erythrocytes is reduced, the blood flow velocity of an organism body is increased, and oxygen utilization by blood of the whole body is promoted; and besides, free radicals accumulated in the organism body are adjusted, mobilized and decomposed, the free radicals in the body are cleared away, metabolism is accelerated, decomposition speed of the liquefied fat cells is increased, and the laser fat reducing effect is greatly improved.

The invention discloses a curcuma zedoary extract and application of the curcuma zedoary extract in preparation of a medicine for preventing and treating coronary heart disease. The curcuma zedoary extract is extracted by the following method: heating and extracting raw or vinegar curcuma zedoary decoction pieces with ethanol; extracting medicine residues with water; and merging the extracting solutions, adding ethanol until the alcohol concentration is 40-60%, standing, filtering, and concentrating the filtrate under reduced pressure until the crude drug content is 0.5-1.0 g/mL. The curcuma zedoary extract can significantly increase the liver index and spleen index of a rat with coronary heart disease, and improve the fatty degeneration, myocardial fibrosis, necrosis and inflammatory infiltration degree of liver cells; indexes such as the whole blood viscosity and the erythrocyte aggregation index of the coronary heart disease rats can be remarkably reduced, and the blood viscous state is reduced; the traditional Chinese medicine composition can significantly improve dyslipidemia, and meanwhile, achieves the treatment effects of relieving vascular cholesterol deposition and inflammation and recovering endothelial functions. Lipiomics results also show that the compound can regulate and control abnormal lipid metabolism of the coronary heart disease by inhibiting sphingolipid metabolism and promoting glyceride metabolism and fatty acyl metabolism.