157 results about "Hemocyte" patented technology

The present invention provides a biosensor that enables highly-accurate measurement of a sample solution including a solid component like hemocytes and has a little variation in response. The biosensor includes: an insulating base plate, an electrode system having at least a working electrode and a counter electrode provided on the base plate, a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and a filter disposed between the electrode system and the sample supply unit in the sample solution supply pathway. The biosensor has a space that encircles surface of the filter in an area from one end of the filter close to the sample supply unit to the other end of the filter close to the electrode system. This arrangement effectively prevents the solid component like hemocytes from flowing into the electrode system without being filtered out by the filter.

A biosensor including an insulating base plate, an electrode system, a cover for covering the insulating base plate, at least one reaction layer, a sample solution supply pathway communicating with an air aperture at a terminal end side, a sample solution supply part and a filter provided between the sample solution supply pathway and the sample solution supply part to filter hemocytes, an end face and a top face of a primary portion of the filter are exposed and the sample solution supply part is provided adjacently to the primary side portion so as to provide an improved biosensor which allows easy addition of whole blood to the sensor and rapid supply of the added whole blood to a filter even in the case of collecting blood by fingertip centesis for measurement.

The invention discloses a method for synthesizing tyrosine kinase inhibitor PCI-32765. The method comprises the following steps of: 1) carrying out a coupled reaction between a compound 10 and a compound 15 to obtain a compound 6; 2) reacting the compound 6 with a compound 16 to obtain a compound 11 in the presence of a more ideal catalyst; 3) protecting the compound 11 to obtain a compound 12; 4) carrying out selective de-protection on the compound 12 to obtain a compound 13; 5) attacking the compound 13 which has only one position which can be attacked by a compound 17 to obtain a very pure compound 14; and 6) removing protecting groups, thus obtaining the PCI-32765. By the method, tyrosine kinase which serves as a main receptor signal of B cells can be inhibited, and the tyrosine kinase inhibitor PCI-32765 is not only capable of inhabiting the generation of hemocyte with little toxicity and side effects, but also is moderate in reaction conditions, simple to operate, convenient to purify, low in cost, environment-friendly, and suitable for large scale production.

The invention provides methods and compositions for the detection and / or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.

In order to provide a cholesterol sensor capable of measuring whole blood with high accuracy and excellent response, where plasma with hemocytes therein filtered promptly reaches an electrode system, in a biosensor comprising: an insulating base plate; an electrode system which is provided on the base plate and has a measurement electrode and a counter electrode; a reaction layer including at least oxidoreductase and an electron mediator; a sample solution supply pathway which includes the electrode system and the reaction layer and has an air aperture on the termination side thereof; a sample supply portion; and a filter which is disposed between the sample solution supply pathway and the sample supply portion and filters hemocytes, where plasma with the hemocytes therein filtered with the filter is sucked into the sample solution supply pathway due to capillarity, the central part of a secondary side portion of the filter is protruded into the sample solution supply pathway more than both the right and left ends thereof.

This invention relates to reconstructed polypeptides which have properties of inducing apoptosis of nucleated blood cells having Integrin Associated Protein (IAP) and causing no hemagglutination. The reconstructed polypeptides comprise two or more H chain V regions and two or more L chain V regions of a monoclonal antibody which induces apoptosis of nucleated blood cells having IAP. The reconstructed polypeptides are useful as a therapeutic agent for blood dyscrasia such as leukemia.

A treatment for systemic DNA mutation diseases accompanied with development of somatic mosaicism and elevation of blood extracellular DNA and, more particularly, to a treatment of diabetes mellitus and atherosclerosis. The inventive method consist from introducing a treatment agent into a circulating blood system of a patient diagnosed with systemic DNA mutation diseases when said treatment agent destroys extracellular DNA in said blood of said patient and wherein said treatment agent used to destroy said extracellular DNA is a DNASE enzyme: said agent might be administered in doses and regimens which sufficient to decrease number average molecular weight of circulating extracellular blood DNA in the blood of said patient; such decrease of number average molecular weight might be measured by gel electrophoresis of extracellular blood DNA fraction from the blood of said patient. A DNASE enzyme may be further applied in a dose and regime that provide a DNA hydrolytic activity measured in blood plasma exceeding 1.5 Kunitz units per 1 ml of blood plasma for more than 12 hours within a period of 24 hours.

The present invention provides a biosensor that enables highly-accurate measurement of a sample solution including a solid component like hemocytes and has a little variation in response. The biosensor includes: an insulating base plate, an electrode system having at least a working electrode and a counter electrode provided on the base plate, a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and a filter disposed between the electrode system and the sample supply unit in the sample solution supply pathway. The biosensor has a space that encircles surface of the filter in an area from one end of the filter close to the sample supply unit to the other end of the filter close to the electrode system. This arrangement effectively prevents the solid component like hemocytes from flowing into the electrode system without being filtered out by the filter.

In a biosensor comprising an insulating base plate, an electrode system including a measuring electrode and a counter electrode provided on said base plate, a cover for covering said insulating base plate, at least one reaction layer containing oxidoreductase and / or an electron mediator, a sample solution supply pathway including said electrode system and said reaction layer and communicating with an air aperture at a terminal end side, a sample solution supply part and a filter provided between said sample solution supply pathway and said sample solution supply part to filter hemocytes, an end face and a top face of a primary portion of the filter are exposed and the sample solution supply part is provided adjacently to the primary side portion so as to provide an improved biosensor which allows easy addition of whole blood to the sensor and rapid supply of the added whole blood to a filter even in the case of collecting blood by fingertip centesis for measurement.

The monoclonal antibodies of this invention are antibodies that specifically recognize human Integrin Associated Protein, and the antigens that induce apoptosis of nucleated blood cells having human Integrin Associated Protein. Accordingly, they are useful as antibodies that recognize human Integrin Associated Protein for its distinction and identification, while also having an action of inducing apoptosis of nucleated blood cells; these properties can be utilized to prepare useful therapeutic agents in the field of treatment for myeloid leukemia and lymphoid leukemia.

Intravenous administration of bone marrow cells collected from rat bone marrow to a rat cerebral infarction model was found to be effective in treating cerebral infarction. Human and murine bone marrow stem cells showed similar effects. Mesenchymal cells such as bone marrow cells, cord blood cells, or peripheral blood cells can be used as agents for in vivo administration against cranial nerve diseases.

The invention relates to premixed composite feed for improving eggshell quality of a hyline brower laying hen. The premixed composite feed comprises the following components in parts by weight: calcium hydrophosphate, rock flour, salt, sodium bicarbonate, choline, composite trace element, composite vitamin, lysine, methionine, fish meal, hemocyte protein powder, corn germ meal, probiotics and phytase. The invention can effectively improve the eggshell color and the eggshell intensity and reduce the egg damage rate by 3-5 percent by combining various technologies, preparing various vitamins and trace elements independently and combining the proportion and dosage of the hemocyte protein powder, the sodium bicarbonate, organic iron, and the like, thereby solving the problems of poor eggshellcolor, poor eggshell quality, and the like generated after 200-250 days of egg laying along with the ageing of laying hens in days in the laying hen culture and bringing great economic benefits to culturists.

The invention discloses a blood sample collecting device. The collecting device comprises a collecting tube, wherein an orifice of the collecting tube is provided with a sealing rubber plug, the outside of the rubber plug is provided with a protective cap, the collecting tube is filled with additives, the additives are composed of a blood anticoagulation, a hemocyte nucleic acid stabilizing agent, a blood plasma nucleic acid stabilizing agent, and a pH buffer solution; the blood anticoagulation is one or more of oxalate, heparinate and citrate, the pH buffer solution is one of glycine-sodium hydroxide-hydrochloric acid buffer solution; the hemocyte nucleic acid stabilizing agent is one or more of allantoin, 5,5-dimethyl hydantoin and oxazolidine; the blood plasma nucleic acid stabilizing agent is N-acetic acid azomethine, N-(3-acetic acid-amylamine)azomethine. The blood sample collecting device simultaneously contains the hemocyte nucleic acid stabilizing agent and the blood plasma nucleic acid stabilizing agent, and does not contain free aldehyde material, so that the blood sample can be stored in long term at normal temperature, and the free nucleic acid level of the blood sample can be stabilized, and the completeness of the free nucleic acid can be maintained.

The invention belongs to the technical field of hemocyte analysis, and relates to a hemocyte analysis chip, a hemocyte analyzer and a hemocyte analysis method. The hemocyte analysis chip comprises a leucocyte / ferrohemoglobin analysis chip and an erythrocyte / thrombocyte analysis chip; the leucocyte / ferrohemoglobin analysis chip comprises a quartile group cell analysis chip and a basophils analysis chip; the quartile group cell analysis chip performs cell sorting by utilizing a chemical dyeing method, an electrical impedance technology and a light adsorption method, and detects haematoglobin by adopting a colourimetry; the basophils analysis chip performs leucocyte counting and basophile detection by utilizing a bio-dissolution technique, an electric impedance measurement method and a threshold landmark method; the erythrocyte / thrombocyte analysis chip is used for counting erythrocytes and thrombocytes by adopting an electric impedance technology and a landmark technology. The hemocyte analysis chip, the hemocyte analyzer and the hemocyte analysis method has the advantages of simple structure, small size, low cost, high convenience for operation, high easiness maintenance and transportation, and disposable chip and the like.

The invention discloses a rare cell multistage separation microfluidic device integrated with an inertial and deterministic lateral displacement technology. One end of the integrated device is provided with a sample inlet; the bottom of the sample inlet is communicated to a spiral runner; the other end of the spiral runner is communicated with a sudden expansion structure; the other end of the sudden expansion structure is divided into a first branch runner and a deterministic lateral displacement runner; the tail end of the first branch runner is provided with a hemocyte outlet; the other endof the deterministic lateral displacement runner is divided into a second branch runner and a third branch runner; the tail end of the second branch runner is provided with a residual hemocyte outlet; and the tail end of the third branch runner is provided with a rare cell outlet. By integrating two stages of runners: the inertial spiral runner and the deterministic lateral displacement runner, the rare cell multistage separation microfluidic device fully makes use of the high flux advantage of an inertial spiral and the high accuracy advantage of a deterministic lateral displacement; furthermore, the device is ingeniously integrated with a simple structure basis to realize high flux and high purity sorting of rare cancer cells; and the shortcoming that an existing sorting chip hardly simultaneously realizes the high flux and high purity sorting in only accordance with a certain single technology is overcome.

A methodology that allows for highly efficient transfer and stable integration of DNA into both established eukaryotic cell lines and primary cells, including non-dividing cells such as human peripheral blood monocytes and macrophages, entails the use of a synthetic polypeptide comprised of a peptide domain which corresponds to a nuclear localization signal sequence and a DNA binding domain which is rich in basic amino acids, separated by a hinge region of neutral acid which prevents stearic interference between the two domains.A synthetic polypeptide that allows for highly efficient transfer of DNA into eukaryotic cells, including, for example, non-dividing cells such as human peripheral blood monocytes and macrophages.<?insert-end id="INS-S-00001" ?>

The invention discloses a hemocyte analyzer hemolytic agent. Every 1L of hemolytic agent comprises 5-40.0g of quaternary ammonium salt, 5.0-25.0g of alkali metal chloride, 0.05-5.0g of ethylenediamine tetraacetic acid or ethylenediamine tetraacetic acid derivative, 0.5-5.0ml of inorganic acid, 0.5-8.0g of pH regulator for regulating the pH value to 5.0-8.0, and the balance of water. The invention solves the problem that the peak shapes of lymphocyte, monocyte and granulocyte in a leucocyte histogram of XFA6000-series and XFA6000 Intelligent-series full-automatic hemocyte analyzers produced by Nanjing Pulang Medical Equipment CO, LTD are not obvious or only the wave peaks of the lymphocyte and granulocyte can appear, and also solves a series of problems of insufficient accuracy of classification of leucocytes; and the reagent does not contain any preservative or cyanide, and thus, does not pollute the environment.

The invention provides methods and compositions for the detection and / or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and / or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.

The invention provides a method using a hemocyte preparation, for example, Limulus amebocyte lysate, for detecting in a single assay the presence of at least one of a Gram negative bacterium, a Gram positive bacterium, and a fungus in a sample of interest. The method exploits the differential reactivity of Gram negative bacteria, Gram positive bacteria, and fungi with the hemocyte preparation to produce measurable changes in a property, for example, an optical property, of the mixture. Because the Gram negative bacteria, Gram positive bacteria and fungi each produce different changes in a given property, for example, an optical property, it is possible to classify the type of microorganism present in the sample of interest.

The invention discloses a hemocyte analysis sensor. Multidimensional diffusion light intensity information is imaged on a CCD sensor array plane in different space fields through combination of various specially-produced optical reflection and transmission parts by using single cells to be detected in a laser irradiation liquid flow system, diffusion light intensity distribution in forward, lateral and backward corresponding solid angle ranges are simultaneously received on the CCD array plane, and light information collected in the invention is analyzed and calculated according to the calculation result of the light diffusion distribution under a hemocyte model by using a light diffusion theory in order to realize classification and counting of a hemocyte group. The sensor has the advantages of abundant diffusion information, full classification basis, small structure and high integration, is suitable for developing apparatuses in the fields of hemocyte classification and cytodynamics analysis scientific researches, and is especially suitable for developing portable apparatuses to meet people's community popularization of health state and disease diagnosis.

The invention relates to the technical field of medicines and biology and particularly relates to a ddPCR detection method for lung cancers EGFR L858R and 19Del and application of the ddPCR detectionmethod. The detection method comprises the steps of separating hemocyte and plasma of peripheral blood, carrying out cfDNA extraction by virtue of a two-step purification method, respectively designing two probes and one pair of primers aiming at lung cancer-relevant EGFR L858R loci and EGFR 19# exon deletion loci, carrying out ddPCR detection by taking extracted cfDNA as a template, and finally carrying out data analysis on an obtained amplification product, so as to obtain the proportion of EGFR sensitively-mutated L858R and 19Del. The application comprises the application of the ddPCR detection method in the treatment of tumor patients, medication-relevant genetic locus mutation information is provided for the tumor patients in the treatment process of the terminal lung cancer, and important reference frame is provided for the individualized accurate medication guidance, pesticide effect evolution and treatment effect monitoring of the lung cancers.

The invention provides methods and compositions for the detection and / or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and / or quantification of the Gram positive bacterial contaminant.

The invention relates to a pediococcus pentosaceus G11 strain as well as screening and applications of the pediococcus pentosaceus G11 strain. The pediococcus pentosaceus G11 strain is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC NO:2016248. The pediococcus pentosaceus G11 strain has tolerance to compound sulfamethoxazole and ceftazidime, has antibacterial activity for vibrio parahaemolyticus, aeromonas hydrophila, vibrio alginolyticus, staphylococcus aureus and beta streptococcus, has certain stress resistance and enzyme producing ability, is free of hemocyte solubility, and has no drug tolerance for most antibiotics; the dipping bath experiment proves that the pediococcus pentosaceus G11 strain is harmless to the host, can improve the immunity of the body, has wide application prospects, can be applied to probiotic preparations, and also can be prepared into bacterial powder which is then added into a feed as a feed additive, further, the growth of pathogenic bacteria in the bodies of the animals is inhibited, so that the death rate of animals is reduced, and the pediococcus pentosaceus G11 strain is a probiotic brain worthy of development.

The invention provides a Chinese medicinal preparation for preventing and treating radiation and a preparation method thereof and relates to the Chinese medicinal preparation for treating and preventing radiation injury and the preparation method thereof. The Chinese medicinal preparation is specifically prepared from heterophylly falsestarwort root, indian buead, bighead atractylodes rhizome, liquorice, Szechuan lovage rhizome, Chinese angelica, red paeony root, rehmannia root, barbed skullcap herb, honeysuckle, mulberry leaf, hairyvein agrimonia herb, medlar and green tea in a certain proportion. Pharmacodynamic experiments carried out by adopting an animal model with reduced tangible hemocytes and nucleated cells in bone marrow of a white mouse due to 60Co-gamma rays show that the preparation has a good effect on hemocytes and hemopoietic tissues injured by nuclear radiation.

The invention relates to a protecting agent for free DNA in plasma and belongs to the biotechnical field. The protecting agent for free DNA in plasma is a sterile aqueous solution comprising the following components: a 50-600 g / L preservative, a 30-100 g / L nuclease inhibitor, a 20-200 g / L metabolic inhibitor and a 1-120 g / L anti-adsorbent. By mixing collected blood with the protecting agent in a proportion of (5-1000): 1, the free DNA of blood can be kept for 14 days in a constant temperature condition, the free DNA of blood can be kept for 4 days at 37 DEG C, and the free DNA of blood can betransported continuously for two days. By optimizing the components and the contents thereof, free nucleic acid in blood can be stored and extracted effectively, and meanwhile, nucleic acid doped intoblood cells as a result of splitting decomposition of hemocyte is avoided after blood is extracted.