The invention discloses a
rare cell multistage separation microfluidic device integrated with an inertial and deterministic
lateral displacement technology. One end of the integrated device is provided with a sample inlet; the bottom of the sample inlet is communicated to a spiral runner; the other end of the spiral runner is communicated with a sudden expansion structure; the other end of the sudden expansion structure is divided into a first
branch runner and a deterministic
lateral displacement runner; the
tail end of the first
branch runner is provided with a
hemocyte outlet; the other endof the deterministic
lateral displacement runner is divided into a second
branch runner and a third branch runner; the
tail end of the second branch runner is provided with a residual
hemocyte outlet; and the
tail end of the third branch runner is provided with a
rare cell outlet. By integrating two stages of runners: the inertial spiral runner and the deterministic lateral displacement runner, the
rare cell multistage separation microfluidic device fully makes use of the
high flux advantage of an inertial spiral and the high accuracy
advantage of a deterministic lateral displacement; furthermore, the device is ingeniously integrated with a simple structure basis to realize
high flux and high purity sorting of rare
cancer cells; and the shortcoming that an existing sorting
chip hardly simultaneously realizes the
high flux and high purity sorting in only accordance with a certain single technology is overcome.