Hemocyte analysis sensor

Abstract

The invention discloses a hemocyte analysis sensor. Multidimensional diffusion light intensity information is imaged on a CCD sensor array plane in different space fields through combination of various specially-produced optical reflection and transmission parts by using single cells to be detected in a laser irradiation liquid flow system, diffusion light intensity distribution in forward, lateral and backward corresponding solid angle ranges are simultaneously received on the CCD array plane, and light information collected in the invention is analyzed and calculated according to the calculation result of the light diffusion distribution under a hemocyte model by using a light diffusion theory in order to realize classification and counting of a hemocyte group. The sensor has the advantages of abundant diffusion information, full classification basis, small structure and high integration, is suitable for developing apparatuses in the fields of hemocyte classification and cytodynamics analysis scientific researches, and is especially suitable for developing portable apparatuses to meet people's community popularization of health state and disease diagnosis.

Description

technical field [0001] The invention belongs to the technical field of blood cell analysis, and in particular relates to an optical sensor which utilizes light scattering theory to integrate multi-dimensional scattering information of samples into an array, and realizes the classification and counting of whole blood cells according to the light information distribution. Background technique [0002] Cell analyzers are widely used in all aspects of basic medical research and clinical practice due to their fast, flexible, mass and quantitative characteristics. The light scattering of biological cells is mainly caused by the diffraction of light at the edge of the cell and the nucleus, the different refractive indices of the cytoplasm, the nucleus and the surrounding medium, the reflection of different optical boundaries, and the absorption in the cell. Therefore, the light scattering pattern of biological cells contains rich information about cell size, internal structure, con...

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Problems solved by technology

[0004] At present, the traditional blood cell analyzers involving light scattering technology need to place light detectors in different positions of the cells to receive the light scattering signals, so the detection light path used is divergent, which is not conducive to the further miniaturization of the analysis instrument. In the method of obtaining information from cells by using scattering technology, the use of backscattering of cells is very little; the use of multiple detectors wastes resources, greatly reduces the use efficiency of products, and also makes the assembly of instruments Complicated and difficult debugging process

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