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Hemocyte analyzer hemolytic agent

The technology of a blood cell analyzer and a hemolytic agent is applied in the field of reagents for medical diagnostic instruments, which can solve the problems of instrument software identification errors, insufficient monocyte peaks, etc., and achieve the effect of obvious peak shape.

Inactive Publication Date: 2013-09-25
NANJING PERLONG MEDICAL EQUIP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Chinese patent 1247982 has reported the reagent that is used for the measurement of hemoglobin and the mensuration of white blood cell in the blood sample, discloses the hemolytic agent that is used to measure leukocyte grouping, but this hemolytic agent obtains in the leukocyte histogram (see figure 1 ), the peak of monocytes (Mo) is not obvious enough, which may easily cause errors in the identification of the instrument software

Method used

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  • Hemocyte analyzer hemolytic agent
  • Hemocyte analyzer hemolytic agent
  • Hemocyte analyzer hemolytic agent

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Diluent:

[0030] Sodium chloride 8.12g / L, disodium hydrogen phosphate 11.2g / L, sodium dihydrogen phosphate 1.9g / L, dipotassium edetate 0.5g / L, pyridinium copper 0.5g / L, the rest is water, pH 7.5.

[0031] Hemolytic agent:

[0032] Decyltrimethylammonium chloride 25.8g / L, tetradecyltrimethylammonium bromide 1.7g / L, sodium chloride 9.2g / L, 1M hydrochloric acid 0.5ml / L, ethylenediaminetetraacetic acid di Sodium 2.0g / L, sodium hydroxide 1.2g / L, the rest is water, pH 6.0.

Embodiment 2

[0034] Thinner:

[0035] Potassium chloride 12.44g / L, boric acid 3.2g / L, borax 1.4g / L, edetate disodium 1.5g / L, pyridinium sodium 0.4g / L, the rest is water, pH 7.4.

[0036] Hemolytic agent:

[0037] Dodecyltrimethylammonium chloride 22.4g / L, cetyltrimethylammonium bromide 2.2g / L, sodium chloride 8.6g / L, formic acid 1.0ml / L, ethylenediaminetetraacetic acid Disodium 1.5g / L, sodium hydroxide 1.2g / L, the rest is water, pH value 5.6.

Embodiment 3

[0039] Thinner:

[0040] Potassium chloride 5g / L, disodium hydrogen phosphate 5.04g / L, sodium dihydrogen phosphate 38g / L, edetate disodium 0.05g / L, pyridinium sodium 0.01g / L, the rest is water, pH value 6.0.

[0041] Hemolytic agent:

[0042] Tetradecyltrimethylammonium chloride 38.0g / L, octadecyltrimethylammonium bromide 2.0g / L, sodium chloride 25g / L, formic acid 5.0ml / L, ethylenediaminetetraacetic acid di Sodium 5g / L, sodium hydroxide 8g / L, the rest is water, pH value 8.0.

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Abstract

The invention discloses a hemocyte analyzer hemolytic agent. Every 1L of hemolytic agent comprises 5-40.0g of quaternary ammonium salt, 5.0-25.0g of alkali metal chloride, 0.05-5.0g of ethylenediamine tetraacetic acid or ethylenediamine tetraacetic acid derivative, 0.5-5.0ml of inorganic acid, 0.5-8.0g of pH regulator for regulating the pH value to 5.0-8.0, and the balance of water. The invention solves the problem that the peak shapes of lymphocyte, monocyte and granulocyte in a leucocyte histogram of XFA6000-series and XFA6000 Intelligent-series full-automatic hemocyte analyzers produced by Nanjing Pulang Medical Equipment CO, LTD are not obvious or only the wave peaks of the lymphocyte and granulocyte can appear, and also solves a series of problems of insufficient accuracy of classification of leucocytes; and the reagent does not contain any preservative or cyanide, and thus, does not pollute the environment.

Description

technical field [0001] The invention belongs to the field of reagents for medical diagnostic instruments, in particular to a hemolytic agent for blood cell analyzers. Background technique [0002] Diluent and hemolyzing agent are the most commonly used reagents for hematology analyzers in medical testing, and are the key reagents for determining the count or classification of hemoglobin (HGB) and white blood cell (WBC). Under the cooperation of the diluent, the hemolytic agent dissolves the red blood cells (RBC) rapidly on the one hand, releases hemoglobin and combines with the hemolytic agent to form a stable hemoglobin derivative, which can be used to measure HGB; on the other hand, the white blood cells maintain their original shape and Keep a certain volume for WBC counting, and classify leukocytes into three groups according to their size: lymphocytes (LYM), monocytes (Mo), and granulocytes (GRA). [0003] Usually, blood cell analysis is to dilute the blood sample with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 尹小东管中来
Owner NANJING PERLONG MEDICAL EQUIP
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