35 results about "Cell analyzer" patented technology

The invention discloses a hematology analyzer and a platelet numerical value measuring method, in the hematology analyzer, an analysis unit is used for receiving a pulse signal to generate a first image, receiving an optical signal to generate a second image, obtaining a platelet original numerical value based on the first image, obtaining an interference coefficient of a leukocyte fragment based on the second image, and obtaining the platelet numerical value based on the interference coefficient; and obtaining a platelet measurement value based on the platelet original value and the interference coefficient. Through the cooperation of the impedance module and the flow fluorescence module, the platelet original value in the blood cell sample and the interference coefficient of the leukocyte fragment are detected, and the accuracy of each step in the calculation process of the platelet measurement value is ensured. Meanwhile, the platelet measurement numerical value is calculated through the analysis unit based on the platelet original numerical value and the interference coefficient, so that the accuracy of the blood cell analyzer for measuring the platelet measurement numerical value is improved, the practicability of the blood cell analyzer is improved, and the use experience of a user on the blood cell analyzer is improved.

The invention relates to the technical field of electromagnetic compatibility (EMC) design of electronic circuits, in particular to a method suitable for a flow cytometer and capable of reducing interference of clock signals on EMC. The method comprises the steps that S100, clock circuit components are concentrated in a 5mm * 5mm area of the top layer of a PCB, routing is limited to a second layer through buried holes, and fundamental frequency is dynamically set; s200, laying copper in a key area for grounding, and adding a ground bounce suppression array to three layers; s300, arranging an upper ground copper layer and a lower ground copper layer by using a flexible circuit board; s400, covering the top circuit with a ferrite alloy cover, and welding and grounding; and S500, the flow velocity and the cell density are collected in real time, the clock frequency is dynamically adjusted, a frequency stabilization mechanism exists in an extreme scene, radiation is effectively reduced, and the detection precision and stability are improved. According to the method, the EMC interference of the clock signal of the flow cytometer is reduced from multiple dimensions of source inhibition, shielding protection, connection optimization and cooperative control.

The invention belongs to the field of medicines, and particularly relates to an in-vitro evaluation model for promoting skin wound healing based on a VPA-Exo drug-loaded exosome as well as a construction method and application of the in-vitro evaluation model. The construction method of the in-vitro evaluation model for promoting skin wound healing comprises the following steps: S1, after passage of resuscitated HSF cells and HUVEC cells, taking HSF cells or HUVEC cell suspension in a logarithmic phase, inoculating the HSF cells or HUVEC cell suspension on a culture plate, and culturing on a real-time label-free cell analyzer for 20-28 hours; and S2, then adding VPA-EXO with the concentration of 12.5 mu g / mL-400 mu g / mL to act on the HSF cells or the HUVEC cells.

The invention discloses a hematology analyzer and a detection method for hole blockage of a flow chamber, in the hematology analyzer provided by the invention, in the process that sample liquid enters the flow chamber for sample detection, a control unit can also obtain signal parameters based on optical signals of the sample liquid flowing through the flow chamber; whether the flow chamber is blocked or not is judged, the safety of the flow chamber is improved, and the detection efficiency of an optical detection system on sample liquid is improved. Meanwhile, in the process of detecting the sample liquid, whether the flow chamber has the hole blocking problem or not can be judged, the step of judging the hole blocking of the flow chamber does not need to be additionally set, the operation efficiency of the hematology analyzer is improved, and the use experience of a user on the hematology analyzer is improved.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, the first bearing position is arranged on the sample injection module to bear the detection card for receiving the first to-be-detected sample, and the second bearing position is arranged on the first detection module to bear the detection card after receiving the first to-be-detected sample, so that the receiving and detection of the first to-be-detected sample are separately carried out at different bearing positions of different modules; the transfer module is arranged at the output end of the driving module of the preprocessing module, so that the driving module can drive the transfer module to transfer the detection card from the first bearing position to the second bearing position after driving the pipetting consumable through the loading module to transfer the first to-be-detected sample into the detection card at the first bearing position; therefore, the driving module is ingeniously used for driving the transfer module, and a driving device special for driving the transfer module is avoided, so that the overall structure of the analyzer is simpler, the production cost is reduced, and the analyzer is more compact and miniaturized in structure.

The invention is applicable to the field of cell analysis equipment, and discloses an optical detection device and a cell analyser, the optical detection device comprises a flow chamber, a light source and a forward scattering detection assembly, the forward scattering detection assembly is used for collecting forward scattering signals generated when the light source irradiates to-be-detected cells, and the forward scattering signals are sent to the flow chamber; the forward scattering detection assembly comprises a first focusing lens, a diaphragm assembly and a first detector, and the diaphragm assembly is arranged between the first focusing lens and the first detector; the first focusing lens is arranged between the flowing chamber and the diaphragm assembly, so that forward scattering light generated when the light source irradiates to-be-detected cells is converged and then penetrates through the diaphragm assembly to enter the first detector, the first focusing lens is a spherical lens, and the number of the first focusing lens is one. The single spherical lens is adopted to collect forward scattering signals and gather the forward scattering signals to the first detector, so that the size and the cost of the optical detection device are effectively reduced, and the miniaturization design of the cell analyzer is facilitated.

This application provides a method for using a POCT hematology analyzer, comprising: in response to a reagent kit being placed in a test chamber, controlling a pipette to move above the first mounting head reservoir of the reagent kit and descending to load the first mounting head; controlling the pipette to move to the diluent reservoir of the reagent kit to transfer the diluent into the pre-cell for impedance detection; controlling the pipette to transfer a sample into the pre-cell; mixing the test solution in the pre-cell; controlling the metal shielding cap to close with the test chamber to establish a connection between the rear cell and the pressure building system of the reagent kit; controlling the pressure building system to guide the test solution from the pre-cell into the rear cell; and detecting the test solution by impedance detection.

1. The name of the design product: fluorescent in situ imaging cell analyzer. 2. The use of the design product: the design product is used for measuring cell concentration and activity, and belongs to the field of biological automatic analysis instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

This utility model belongs to the technical field of blood cell analyzers, specifically relating to an isolation chamber device for a blood cell analyzer. It includes an isolation chamber body, a waste liquid outlet fixedly connected to the lower outer side of the isolation chamber body, a mixing gas inlet fixedly connected to the upper outer side of the isolation chamber body, a top cover fixedly connected to the upper side of the isolation chamber body, a reaction tank connection port fixedly connected to the upper side of the top cover, a filter body fixedly connected inside the isolation chamber body, a fixed blocking part fixedly connected inside the isolation chamber body opposite to the mixing gas inlet, a buoyancy component and a movable blocking part connected to the buoyancy component, which, when the buoyancy component rises, moves an airbag to the mixing gas inlet to seal it. This utility model can reduce the phenomenon of liquid from inside the isolation chamber body entering the mixing gas inlet.

The invention relates to a blood cell analyzer and a blood cell analysis method. Sucking a to-be-detected blood sample; mixing a part of the blood sample to be detected, a hemolytic agent and a first fluorescent staining agent to prepare a first measurement sample, and detecting first optical information of particles in the first measurement sample; mixing the other part of the blood sample to be measured, the diluent and a second fluorescent staining agent to prepare a second measurement sample, and detecting second optical information of particles in the second measurement sample; acquiring first leukocyte classification information based on the first optical information; when it is judged that classification abnormity exists in the first leukocyte classification scatter diagram, second leukocyte classification information is obtained based on the second optical information, and the second leukocyte classification information is used for correcting the first leukocyte classification information. According to the invention, an accurate leukocyte classification result can be obtained.

This application relates to a blood cell analyzer and a red blood cell detection method. The blood cell analyzer includes: a sampling device for aspirating a blood sample to be tested; a sample preparation device for mixing a portion of the blood sample to be tested with a diluent to prepare a test sample; an impedance detection device including a counting cell and a detection component, wherein the counting cell is for the test sample to pass through, and the detection component is for acquiring the electronic signals of each particle in the test sample as it passes through the counting cell; and a processor configured to: acquire raw red blood cell volume distribution information based on the electronic signals of each particle in the test sample; acquire red blood cell agglutination information based on the raw red blood cell volume distribution information; and obtain the red blood cell count value of the blood sample to be tested based on the raw red blood cell volume distribution information and the red blood cell agglutination information. This enables the acquisition of an accurate red blood cell count value.

The application is suitable for the field of cell analysis equipment, and discloses an optical detection device and a cell analyzer. The optical detection device comprises a flow chamber, a light source and a forward scattering detection assembly. The forward scattering detection assembly is used for collecting a forward scattering signal generated by the light source irradiating on a cell to be measured. The forward scattering detection assembly comprises a first focusing lens, an aperture assembly and a first detector. The aperture assembly is arranged between the first focusing lens and the first detector. The first focusing lens is arranged between the flow chamber and the aperture assembly, so as to make forward scattering light generated by the light source irradiating on the cell to be measured converge and then pass through the aperture assembly to enter the first detector. The first focusing lens is a spherical lens, and the number of the first focusing lens is one. The single spherical lens is used for collecting the forward scattering signal and converging the forward scattering signal on the first detector, so that the volume and cost of the optical detection device are effectively reduced, and the miniaturization design of the cell analyzer is facilitated.

The present invention relates to the technical field of cell analyzers, and discloses an animal cell immunocompetence analyzer, which comprises: a housing, the upper end of which is provided with a display operation screen, the front end of which is provided with a reagent plate docking port, the reagent plate docking port is internally provided with a telescopic reagent plate receiving member for receiving an animal cell reagent plate, the side wall of which is provided with a discharge port, and the side wall of which is correspondingly provided with a collection box; the coaxial integrated bias analysis area is arranged in the shell, and a central positioning column vertically extending is arranged in the center of the coaxial integrated bias analysis area. According to the invention, through the circumferential layout of the coaxial integrated bias analysis area and the equidistant distribution of the plurality of independent incubation units, synchronous incubation and detection of multiple samples are realized; the movable inner cores are accurately conveyed through the transfer conveying piece and the conveying separation piece, the single-time operation efficiency is improved, and the efficiency bottleneck of serial processing of traditional equipment is solved.

The application designs a three-dimensional flow cytometer and method based on optical detection and ICP-MS combination, relates to the technical field of life analysis scientific detection equipment; first, a three-dimensional flow cytometer is provided, a three-dimensional flow cytometer method based on optical detection and ICP-MS combination is designed based on the analyzer, and the flow cytometer can only perform single-parameter flow cytometer analysis of fluorescence, scattering and mass spectrum or multi-parameter flow cytometer analysis in any combination; the data processing software in the analyzer can provide offline processing of flow cytometer analysis data of fluorescence, side scattering light and mass spectrum, identify, calculate and statistically analyze single-cell pulse signals, especially can provide integration and unification among fluorescence, side scattering and mass spectrum single-cell signals, and obtain three-parameter information of the same cell.

The invention relates to the field of medical instruments, and discloses a pressure-flow rate cooperative control method and system, and the method comprises the steps: obtaining standardized operation data of a flow cytometer fluid path; constructing a liquid path sparse Bayesian causal graph structure; performing feature screening and weight addition on the sparse Bayesian causal graph structure of the liquid path to obtain a multi-level risk probability of each time window; adjusting the importance coefficient of nodes in the liquid path sparse Bayesian causality graph structure by using the multi-level risk probability; using a liquid path sparse Bayesian causal graph structure to constrain the reinforcement learning strategy space to obtain a liquid path parameter adjustment suggestion; a liquid path parameter adjustment suggestion is combined with PID control to form a pressure and flow rate dual-target adaptive control instruction set; and executing the adaptive control instruction set, and driving continuous evolution of the liquid path parameter adjustment suggestions. According to the invention, the flow velocity is stable, the repeatability of cell concentration, absolute counting and signal intensity is improved, and high-quality quantitative analysis is supported.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, a first bearing position is arranged on a sample introduction module and used for bearing a detection card, a second bearing position is arranged on a first detection module and used for bearing the detection card, and a first sample to be detected is transferred into the detection card located at the first bearing position through a pretreatment module; and transferring the detection card receiving the first to-be-detected sample at the first bearing position to the second bearing position through a transfer module, and detecting the settled first to-be-detected sample in the detection card at the second bearing position through the microscope unit, therefore, each detection card receives the first to-be-detected sample and the detection of the first to-be-detected sample after settlement is carried out at different bearing positions, so that the detection efficiency of the POCT hematology analyzer can be effectively improved.

The invention relates to the technical field of hematology analyzers, and discloses a cell analyzer sample introduction device which comprises a machine body, a detection cavity, a feeding drawer, a hydraulic cylinder and a sample introduction calibration module, and a bin door is hinged to the front face of the machine body. The stable and controllable driving force is provided through the electric push rod and transmitted to the single rack through the push block, the synchronous gear is matched to synchronously and symmetrically transmit the movement to the rack on the other side, clear guide is provided for placement, stable circumferential constraint is formed after clamping, and the clamping precision is improved. The test tubes are effectively prevented from deviating or shaking in the horizontal direction possibly in the subsequent conveying process, the problem that due to manual placement errors or mechanical conveying gaps, the positions of the test tubes on the horizontal plane are uncertain is solved, and therefore radial dislocation of the test tubes and puncture needles at the final detection station is avoided; the problems that the puncture needle cannot accurately pierce into the preset position of the test tube, sampling fails and even the precise puncture needle is damaged are solved.

The embodiment of the invention relates to the field of hematology analyzers, and discloses a hematology analyzer and a debugging method.The debugging method comprises the steps that first feature values of test standard particles of different sizes on a target hematology analyzer are obtained, and a base line of the target hematology analyzer is calculated; the first characteristic value is an abscissa value which is corresponding to the peak value of the pulse distribution histogram and is used for representing the volume of the test standard particle; calculating a particle amplification degree difference between the target hematology analyzer and the standard hematology analyzer according to the base line and the first characteristic value; and adjusting a pulse distribution histogram generated by the target hematology analyzer according to the difference between the baseline and the particle amplification degree of the target hematology analyzer, so that the measured values of the target hematology analyzer and the standard hematology analyzer for the hematohematocrit or the average hematohematocrit of the same test standard particle are consistent. According to the application, the debugging problem of the hematology analyzer is solved, and the influence of instrument hardware on the pulse measurement value is avoided.

This application discloses a white blood cell detection method for a blood cell analyzer. The method involves using a first detection module to detect white blood cell samples and obtain a white blood cell histogram. The count of basophils is then calculated from the white blood cell histogram. A second detection module then detects the white blood cell samples and obtains a scatter plot showing the two-dimensional distribution of four cell types, yielding the numbers of monocytes, neutrophils, and eosinophils. Finally, the five-part differential ratio of white blood cells is calculated based on the numbers of lymphocytes, monocytes, neutrophils, eosinophils, and basophils. This method can accurately detect various cell types within white blood cells. Furthermore, the calculation method is simple and cost-effective, thus achieving accurate detection results at a low cost.

The utility model discloses a hematology analyzer which comprises a frame body, a power supply assembly, a control circuit board, a reagent supply assembly, an automatic sample injection assembly and a blood routine examination assembly, a first open cavity, a middle wiring cavity and a second open cavity are sequentially formed in the middle of the frame body; the power supply assembly is arranged on the rear end face of the frame body, the control circuit board is arranged at the top of the frame body, the reagent supply assembly is arranged in the second open cavity and on the front end face of the frame body, the automatic sample injection assembly is arranged on the front end face of the frame body, and the blood routine examination assembly is arranged in the first open cavity; the control circuit board is electrically connected with the power supply assembly, the reagent supply assembly, the automatic sample injection assembly and the blood routine examination assembly; the reagent supply assembly is connected with the blood routine examination assembly. According to the utility model, modularized components are adopted, and the components are reasonably arranged, so that the production and assembly efficiency can be improved, and the maintenance is easy.

The invention relates to a blood cell analyzer and a blood cell analysis method. The method comprises the following steps: mixing a part of a sucked blood sample to be detected, a diluent and a fluorescent staining agent in a first reaction tank to prepare a first determination sample, and obtaining first optical information generated after particles in the first determination sample are irradiated by light; mixing the other part of the blood sample to be detected with a hemolytic agent in a second reaction tank to prepare a second determination sample, and obtaining colorimetric optical information of the second determination sample; obtaining a reticulocyte detection result, a platelet detection result, an erythrocyte detection result and a first leukocyte detection result of the to-be-detected blood sample based on the first optical information, wherein the first leukocyte detection result at least comprises a lymphocyte percentage, a mononuclear cell percentage and a granulocyte percentage; and determining the hemoglobin concentration of the to-be-detected blood sample based on the colorimetric method optical information. According to the application, the reagent cost required for obtaining the detection result of the to-be-detected blood sample can be reduced.

The utility model provides a kind of full-automatic flow cytometry, including sample entering module, reagent disc module, reagent needle module, sample sample adding module, dilution disc module, gripper arm group module, incubation disc module, magnetic separation module, consumable disc module, sample loading discarding module, flow detection module, each module cooperates to realize the whole process of sample dilution, sampling distribution, reagent distribution, constant temperature incubation, reaction system cleaning, flow detection and other flow experiments.The full-automatic flow cytometry can automatically complete the pretreatment and detection of flow sample, improve the consistency and reliability of sample pretreatment, reduce detection difference, simultaneously compatible protein marker and cell detection, support protein detection project and cell analysis project without difference on-machine operation, instrument can automatically identify detection project, correspondingly grab different detection tube, execute different program, without artificial distinction, improve detection efficiency.

To acquire cell morphology information, protein information, and genetic information in a short time at a low cost by a single cell analyzer. A cell analysis device according to the present disclosure comprises: a first detector that detects scattered light and fluorescence from cells containing a fluorescent-stained protein introduced into a first flow path; a solution mixing unit in which each cell is enclosed in a microcompartment and mixed with a reaction solution; a temperature adjustment unit that performs an amplification reaction of a gene in a cell in a microcompartment; a second detector that detects fluorescence from the microcompartment; and a processing device. The processing device analyzes the morphological information of the cell based on the scattered light, analyzes a first fluorescent pattern based on fluorescence from the protein, analyzes a second fluorescent pattern based on fluorescence from the protein in the microcompartment, acquires genetic information based on fluorescence from the gene, and associates the morphological information, protein information, and the genetic information of the cell for each cell based on the first fluorescent pattern and the second fluorescent pattern.

Embodiments relate to a blood cell analyzer, a method and use of an infection marker parameter. The blood cell analyzer comprises a sampling device for aspirating a blood sample of a subject to be tested, a sample preparation device for preparing an assay sample, an optical detection device for detecting the assay sample to obtain optical information, and a processor. The processor is configured to obtain a first white blood cell parameter of a first target particle group in a first assay sample from first optical information of the first assay sample, obtain a second white blood cell parameter of a second target particle group in a second assay sample from second optical information of the second assay sample, the first and / or second white blood cell parameter comprising a cell characteristic parameter, obtain an infection marker parameter for assessing an infection status of the subject based on the first white blood cell parameter and the second white blood cell parameter, and output the infection marker parameter. Thereby, an accurate and effective infection marker parameter can be quickly provided to a user to effectively assist the user in assessing the infection status of the subject.

A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.

In a configuration for analyzing data of cells measured by a cell measuring apparatus, accuracy of cell classification is improved without requiring the cell measuring apparatus to have high information processing capability. A cell analysis method, using a cell analyzer for analyzing cells in accordance with an artificial intelligence algorithm, includes: obtaining the data regarding the cells measured by the cell measuring apparatus; analyzing the data to generate information regarding a cell type of each of the cells; and transmitting the information to the cell measuring apparatus.

The utility model belongs to the technical field of blood cell analyzer auxiliary equipment, and particularly relates to a blood sample diluting and uniformly mixing device for a blood cell analyzer, which is mainly composed of a mixing container and a stirring mechanism. The stirring mechanism comprises a driver, a stirring piece, a sliding frame and a transmission assembly, the longitudinal sliding of the sliding frame and the stable transmission of the transmission assembly are utilized, the stirring piece can continuously rotate in the vertical movement process, and all-directional stirring is achieved. The device is further provided with a sliding rod, a limiting rod, an elastic piece and other structures, stable sliding of the sliding frame is guaranteed, a user can conveniently adjust the stirring position, and operation is convenient and fast. In addition, the stirring and diluting tank adopts an up-down through structure, and a detachable plugging piece at the lower end is convenient to clean, so that cross contamination is effectively avoided. The device effectively solves many problems of a traditional blood sample diluting and uniformly mixing mode, and the blood sample uniformly mixing effect and the detection accuracy are remarkably improved.

The utility model relates to the technical field of cell analysis, and provides a sampling device of a hematology analyzer, which comprises an analyzer body, a probe is arranged at the top end of the analyzer body, a bottom plate is arranged at the bottom end of the analyzer body, and positioning blocks are fixedly connected to two sides of the upper surface of the bottom plate. The sides, close to each other, of the two positioning blocks are rotationally connected with the same two-way lead screw, one sides of the positioning blocks are fixedly connected with servo motors, the servo motors penetrate through the positioning blocks and are fixedly connected with one ends of the two-way lead screws, and the arc surface of each two-way lead screw is in threaded connection with two moving blocks. And the inner walls of the two moving blocks are rotationally connected with driving rods. The sampling device of the hematology analyzer solves the technical problem that the overall practicability and the application range of the equipment are influenced as part of catheters cannot be stably fixed and detected due to the lack of the function of adjusting the length of the catheters in the conventional equipment.

1. The name of the design product: full-automatic flow cytometer. 2. The use of the design product: for realizing the automatic pretreatment and flow detection and analysis of samples, covering the detection and analysis of protein and cell biomarkers. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

In a configuration for analyzing data of cells measured by a cell measuring apparatus, accuracy of cell classification is improved without requiring the cell measuring apparatus to have high information processing capability. A cell analysis method, using a cell analyzer for analyzing cells in accordance with an artificial intelligence algorithm, includes: obtaining the data regarding the cells measured by the cell measuring apparatus; analyzing the data to generate information regarding a cell type of each of the cells; and transmitting the information to the cell measuring apparatus.