245 results about "Cell analyzer" patented technology

A system for qualifying cells of a cell sample labeled with a magnetic or magnetizable moiety is provided. The system includes a cell sample holder for holding a cell of the cells and a first cell analyzer which includes a magnetic field source for applying a magnetic field to the cell and a sensor for qualifying and / or quantifying an effect of the magnetic field on the cell.

A lytic reagent composition and the method of use for differential analysis of leukocytes using flow cytometry are disclosed. The lytic reagent composition includes a short chain alkyl oxyethanol, such as 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol, or 2-isopropoxyethanol, in a sufficient amount to preserve leukocytes; a non-lysing nonionic surfactant as a debris solublizer; and an inorganic buffer to maintain pH of the lytic reagent composition in a range from 9.1 to 10.7. The lytic reagent composition is used to lyse red blood cells and preserve leukocytes, as well as used as the sheath reagent for the focus flow measurement. The lytic reagent composition, when used on a flow cytometric analyzer with multiple angle light scatter and light absorbance measurements, enables differentiation of leukocytes into five subpopulations.

Highly accurate and easy discrimination of cancer / atypical cells is achieved. A cancer / atypical cell discrimination method comprises: measuring a plurality of cells by a flow cytometer; acquiring a scattered light signal for each of the cells; calculating at least one characteristic parameter by analyzing the waveform of the scattered light signal; and discriminating a cancer / atypical cell from the plurality of cells based on the characteristic parameter.

A system for qualifying cells of a cell sample labeled with a magnetic or magnetizable moiety is provided. The system includes a cell sample holder for holding a cell of the cells and a first cell analyzer which includes a magnetic field source for applying a magnetic field to the cell and a sensor for qualifying and / or quantifying an effect of the magnetic field on the cell.

The invention discloses a forward scattering optical signal detecting device used by a flow cell analyzer or a particle analyzer, a forward scattering optical signal detecting method and a cell or particle analyzer provided with the device. The forward scattering optical signal detecting device comprises a detecting unit and a collecting unit, wherein the detecting unit is provided with a plurality of subsidiary detecting units arranged in a one-dimensional position and used for detecting the forward scattering optical signals; the collecting unit used for collecting forward scattering optical signals and focusing the forward scattering optical signals on the detecting unit; and the optical signals detected by the subsidiary detecting units in different positions or intervals are used as the optical signals with different scattering angles. The invention can reduce the system cost and keep the miniaturized advantage, is easy to debug and can feely select the collected optical signals with different scattering angles so as to more precisely distinguish all subgroups in cells or particles to be detected.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting blood cells in biological samples. A small unmeasured quantity of a biological sample such as whole blood is placed in the disposable test cartridge which is then inserted into the cell analyzer. The analyzer isolates a precise volume of the biological sample, mixes it with self-contained reagents and transfers the entire volume to an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping, when it is transferred into the imaging chamber. Images of essentially all of the cellular components within the imaging chamber are analyzed to obtain counts per unit volume. The devices, apparatus and methods described may be used to analyze a small quantity of whole blood to obtain counts per unit volume of red blood cells, white blood cells, including sub-groups of white cells, platelets and measurements related to these bodies.

A sample preparation apparatus comprising: a storage chamber that can store therein a liquid sample including an analysis target to be analyzed; a concentrated sample storage chamber that is provided to communicate with the storage chamber and that stores therein concentrated liquid having an analysis target having a higher concentration than that of the liquid sample; and an analysis target transportation section for transporting the analysis target included in the liquid sample stored in the storage chamber to the concentrated sample storage chamber. A cell analyzer is also disclosed.

The present invention is to present a cell analyzer capable of measuring cells which are approximately 20 to 100 [mu]m in size with high precision via flow cytometry. The cell analyzer 10 comprises: a flow cell 51 in which a measurement sample including a measurement target cell flows; a light source part 53 for irradiating a light on the measurement sample flowing in the flow cell 51; an optical system 52 for forming a beam spot on the measurement sample flowing in the flow cell 51, the beam spot having a diameter of 3-8 [mu]m in a flow direction of the measurement sample and a diameter of 300-600 [mu]m in a direction perpendicular to the flow direction of the measurement sample; and a light receiving part 55, 58, 59 for receiving a light from the measurement sample.

The invention discloses a method and a device for collecting a sample by a full-automatic blood cell analyzer, belongs to a blood detection device, and particularly relates to a method and a device for collecting the sample by the full-automatic blood cell analyzer used in a blood routine test. The device comprises a sampling needle, and an inner hole of the sampling needle is connected with a diluent injection device through a pipeline. The method is characterized by comprising the steps that a sampling needle outer wall cleaning element is sleeved outside the sampling needle; a waste fluid outlet and a diluent inlet for cleaning in up and down arrangement are arranged at the lateral wall of a housing of the sampling needle outer wall cleaning element; the waste liquid outlet is connected with a waste liquid collecting device, and the diluent inlet for cleaning is connected with a diluent supply device. The sampling needle is full of diluent; the diluent and a whole blood sample are isolated by an air column; the outer wall of the sampling needle is cleaned by the sampling needle outer wall cleaning element; the front end of the whole blood sample is removed before the whole blood sample is injected; and the inner wall of the sampling needle is cleaned after the whole blood sample is injected, so that the metering accuracy for sample collection is ensured.

Provided is a cell concentration / purification device having a function of successively locating cells in a specific area of a microchannel, and a function of sequentially capturing cell images by use of light from a plurality of monochromatic light sources on an image basis, and performing comparative analysis of the cell images to recognize individual cells based on information on the shape of the cells and an absorption spectral distribution of the cells or inside of the cells, thereby selectively separating / purifying the cells.

A cell processing apparatus 29 of the present invention includes a storage container 57 that can contain liquid L including a biological sample; a filter 60 that prevents a first cell C1 in the biological sample from passing therethrough and that allows a second cell C2 having a smaller diameter than that of the first cell C1 to pass therethrough; and a filtration cylinder 58 for separating, in the storage container 57 and via the filter 60, the liquid L into a first liquid L1 mainly including the first cell C1 and a second liquid L2 mainly including the second cell C2. A measurement target cell discriminated by the filter from the other cells can be easily collected.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100 / 5180 / 5300 / 5380 / 5310 / 5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

The invention discloses a meter assembly, a sheath fluid impedance meter and a flow cytometer. The meter assembly comprises a meter, a waste fluid isolation chamber, a back sheath isolation chamber and a pressure balance pipeline, wherein the meter is provided with a front pool, a back pool, a back sheath inlet and a waste fluid outlet; the front pool and the back pool are communicated through a metering hole; both the back sheath inlet and the waste fluid outlet are communicated with the back pool; the back sheath isolation chamber is connected with the back sheath inlet; the waste fluid isolation chamber is connected with the waste fluid outlet; the back sheath isolation chamber is connected with the ambient atmosphere through a gas pipeline; the pressure balance pipeline is connected with the back sheath isolation chamber and the waste fluid isolation chamber; and the pressure balance pipeline is provided with a pressure balance controller for controlling the opening / closing state of the pressure balance pipeline. In the cleaning and metering process, the closed chamber is arranged above the liquid level of the back sheath isolation chamber, thereby enhancing the stability of the liquid level.

To accurately and easily discriminate cancer and atypical cells. A method which comprises measuring multiple cells with a flow cytometer, acquiring scattered light signals of individual cells, analyzing the wave shape of each scattered light signal to thereby calculate one or more characteristic parameters and then discriminating cancer and atypical cells from among these multiple cells with the use of the characteristic parameters.

The invention discloses an automatic sorting correction method based on shape characteristics for analytical processing of a flow cytometer. The method comprises the following steps: classifying and processing by an automatic sort algorithm to obtain a boundary curve of each cell according to scatter diagram data analyzed by flow cytometry; selecting a curve model equation to fit the boundary curves of each blood cell; computing standard curve parameters obtained by fitting; and comparing the standard parameters with pre-counted parameter empirical scope, judging whether the sort algorithm result is right and prompting. In the automatic sorting correction method based on the shape characteristics, a judgment mode of fitting curve parameters is adopted to compare with the pre-counted parameter empirical scope for correction, thus facilitating counting and graphic processing of cell detection data in the process of flow cell detection with more accurate processing result, and also facilitating the elimination and alarm processing of wrong detection results.

A cell analyzer includes: a measuring portion which measures cells that are nuclear stained; a displaying portion which displays a histogram of a fluorescence intensity by using a result of the measurement by the measuring portion; and a determining unit which obtains a number of strong-area cells that are distributed in an area where the fluorescence intensity is stronger than normal cells, and which determines a malignancy grade of cancer based on the number of strong-area cells and the histogram.

The application discloses a particle analyzing instrument and a liquid path system thereof. The liquid path system includes a large-liquid-discharge-amount injector, a small-liquid-discharge-amount injector, four controllable valves and a plurality of pipelines, wherein the large-liquid-discharge-amount injector is used for not only providing a sheath liquid but also providing a cleaning liquid and a suction sample liquid; and the small-liquid-discharge-amount injector is used for not only collecting a sample but also providing a sample flow so that devices can be used repeatedly. By means of design of the pipelines, number of the controllable valves is saved. All functions of the liquid path system of a flow cytometry analyzing instrument, such as sampling, blood separating, sample preparing, flow cytometry measuring, cleaning and the like, can be completed just by for flow-controlling valves, thereby simplifying the structure of the liquid path system.

The invention discloses a fluorescence detection system and a cell analyzer adopting the fluorescence detection system. The fluorescence detection system comprises a fluorescence collection object lens, a spectroscope, a fluorescence transmission channel and a detector unit. The spectroscope comprises a first double-prism assembly comprising a first prism and a second prism. Parallel light aligned by the fluorescence collection object lens irradiates a first incident surface of the first prism. A second exit surface of the second prism is perpendicular to an optical axis of the fluorescence collection object lens. The first prism is arranged in a way so that incident parallel light is reflected by the first prism and the second prism, then is divided into multiple parallel lights which have different wavelengths and are parallel to the incident parallel light, and then exit from the second exit surface. The fluorescence detection system can flexibly satisfy wave band demands and is convenient for fluorescence collection in subsequent channels. The number of the fluorescence transmission channels can be flexibly adjusted according to actual requirements.

A counter assembly, sheath flow impedance count device, and flow cytometer analyzer are disclosed.

An assay technique for label-free, highly parallel, qualitative and quantitative detection of specific cell populations in a sample and for assessing cell functional status, cell-cell interactions and cellular responses to drugs, environmental toxins, bacteria, viruses and other factors that may affect cell function. The technique includes a) creating a first array of binding regions in a predetermined spatial pattern on a sensor surface capable of specifically binding the cells to be assayed; b) creating a second set of binding regions in specific spatial patterns relative to the first set designed to efficiently capture potential secreted or released products from cells captured on the first set of binding regions; c) contacting the sensor surface with the sample, and d) simultaneously monitoring the optical properties of all the binding regions of the sensor surface to determine the presence and concentration of specific cell populations in the sample and their functional status by detecting released or secreted bioproducts.

The invention relates to the field of a medical cell analyzer, in particular to a signal effectiveness analysis method and device applied to a cell analyzer. The analysis method comprises the steps of acquiring particle / cell data by a signal acquisition channel, grouping the particle / cell data to obtain more than two groups of particle / cell segmented signals, and sorting the obtained signals; carrying out statistics on all segmented pulse parameters; calculating segmented credible coefficients and segmented variation trends according to all the segmented pulse parameters; and then, comparing the segmented credible coefficients and the segmented variation trends with preset threshold values to judge whether the data is abnormal or not. After the method and the device are adopted, the acquired overall data can be processed in a segmented way, so that the effectiveness of the acquired digital signals can be accurately analyzed; whether the data is abnormal or not can be judged on the premise of not prolonging the measurement time and not increasing the sample amount, so that the reliability of the measuring result can be improved.

A quality control material and a calibrator for calibrating blood cell analyzers comprise simian whole blood plasma, anticoagulant, preservation solution, cell mimics and stabilizer, wherein the weight ratio of the simian whole blood plasma to the anticoagulant is 10:1, the weight ratio of the mixture of the simian whole blood plasma and the anticoagulant to the preservation solution is 10:1, 1 percent by weight of stabilizer is added into the mixture of the three components, and the cell mimics are added according to the amount and concentration of products. The prices of the quality control material and the calibrator are reasonable, the quality of the quality control material and the calibrator is reliable, and the quality control material and the calibrator meet national sanitary requirement, and also can more accurately test the precision and stability of a blood cell analyzer.

The invention discloses a hemocyte analyzer hemolytic agent. Every 1L of hemolytic agent comprises 5-40.0g of quaternary ammonium salt, 5.0-25.0g of alkali metal chloride, 0.05-5.0g of ethylenediamine tetraacetic acid or ethylenediamine tetraacetic acid derivative, 0.5-5.0ml of inorganic acid, 0.5-8.0g of pH regulator for regulating the pH value to 5.0-8.0, and the balance of water. The invention solves the problem that the peak shapes of lymphocyte, monocyte and granulocyte in a leucocyte histogram of XFA6000-series and XFA6000 Intelligent-series full-automatic hemocyte analyzers produced by Nanjing Pulang Medical Equipment CO, LTD are not obvious or only the wave peaks of the lymphocyte and granulocyte can appear, and also solves a series of problems of insufficient accuracy of classification of leucocytes; and the reagent does not contain any preservative or cyanide, and thus, does not pollute the environment.

The invention provides an asymmetric cyanine compound, a preparation method and the application thereof. The asymmetric cyanine compound is shown as a formula I, wherein X, n, R1, R2, R3, R4 and Y are defined in instructions. The maximum absorption peak position of the asymmetric cyanine compound is close to 640 nm and does not vary with environmental temperature; after being combined with nucleic acid, a dye / nucleic acid compound with rapidly enhanced fluorescence intensity is used as a nucleic acid dye in a streaming cell analyzer, and a spectrum in a near infrared region can effectively reduce the interference of background fluorescence and can improve the detection precision. Meanwhile, the compound can be used as a dye of reticular cells in the blood.

Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting blood cells in biological samples. A small unmeasured quantity of a biological sample such as whole blood is placed in the disposable test cartridge which is then inserted into the cell analyzer. The analyzer isolates a precise volume of the biological sample, mixes it with self-contained reagents and transfers the entire volume to an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping, when it is transferred into the imaging chamber. Images of essentially all of the cellular components within the imaging chamber are analyzed to obtain counts per unit volume. The devices, apparatus and methods described may be used to analyze a small quantity of whole blood to obtain counts per unit volume of red blood cells, white blood cells, including sub-groups of white cells, platelets and measurements related to these bodies.

The invention relates to a method and device for diluting a concentrated diluent used by a blood cell analyzer. The device comprises an upper water tank (8) connected with a lower water tank (11) through a connecting solenoid valve (9), wherein the upper water tank (8) is connected with a pure water maker (2) through a water injection solenoid valve (3) and a concentrated diluent bag (13) through a peristaltic pump (16); an upper water tank liquid level probe (14), a stirring device (22), a detecting system and a constant temperature system are arranged on the upper water tank (8); the detecting system is composed of a conductivity detecting electrode (15) and a PH meter probe (19); the constant temperature system is composed of a heater (23), a temperature protection switch (5) and a temperature sensor (6); and a lower outlet (12) of the lower water tank (11) is connected with a diluent inlet (1) of the blood cell analyzer through a pipe and is provided with a lower water tank liquid level floater sensor (10). The concentrated diluent in small bags can be automatically diluted into common diluent applicable to the analyzer at a user terminal, so that the logistics cost is greatly saved, and the user experience is not influenced through the automatic diluting process.

The invention relates to a method for carrying out automatic classified counting on cells in human blood, which comprises the following steps: (1) generating a two-dimensional scatter diagram on two one-dimensional data; (2) carrying out smooth treatment on the two-dimensional scatter diagram so as to make points at the border of the class and the main body part of the class continuous, and eliminating distant discrete points; (3) clustering and searching a class center by taking the two-dimensional scatter diagram after smooth treatment as a foundation, and classifying and labeling the cells; (4) counting the cells in the positions with the same label, and calculating the percentage of each class of cells accounting for the total cells. The method disclosed by the invention can be used for carrying out adaptive classification based on data characteristics, the border of each class can be automatically adjusted according to the characteristics of the scatter diagram of a blood sample, the defects that fixed classification cannot be adjusted according to blood characteristics and the efficiency of manual border adjustment is low are overcome, and the method can be used in an automatic blood cell analyzer for rapid analysis.

The invention relates to a yellow mushroom standardized component manufacturing method. The method comprises the following steps: (1) drying and crushing yellow mushrooms, then performing water extraction, centrifugally collecting mushroom residues, extracting the mushroom residues with acetone, centrifugally collecting filtrate, and performing reduced pressure concentration on the filtrate to form paste, namely an acetone extract; (2) dissolving the acetone extract in an n-hexane-ethyl acetate mixed solution, then filtering to obtain a sample solution containing the acetone extract, and separating the sample solution containing the acetone extract on a preparation chromatogram to obtain 14 standardized component samples, namely Fr1, Fr2,..., Fr13 and Fr14; and (3) performing anti-lung cancer cell model screening on the 14 standardized component samples in vitro by an iCelligence real-time label-free cell analyzer, thus determining that the 7th, 8th and 14th standardized components have human lung cancer cell adherence and proliferation inhibition activity in vitro. The yellow mushroom standardized component manufacturing method provided by the invention has the advantages of simple process and easiness in implementation. Simultaneously, the obtained active components can be applied to treatment of lung cancer.

The invention, which belongs to the medical inspection technology and the special multifunctional rapid detection device and technology for novel device-clinical medical physiology, biochemistry and pathological examination, discloses a forward-scattering light signal detection and collection system of a flow cell analyzer and a multi-angle detection method thereof. The system comprises a fluid flow unit, a light source unit, a first speed-measuring focusing imaging unit, a second speed-measuring focusing imaging unit, and a third speed-measuring focusing imaging unit. The light source unit, the first speed-measuring focusing imaging unit, the second speed-measuring focusing imaging unit, and the third speed-measuring focusing imaging unit are distributed at the circumference using the fluid flow unit as the center uniformly; a cell suspension in the liquid flow unit flows through an imaging detection area side by side at a constant speed; scattered light entering the cell suspension at different angles enters the first speed-measuring focusing imaging unit, the second speed-measuring focusing imaging unit, and the third speed-measuring focusing imaging unit respectively to obtainthe movement speed, a defocusing amount, a bright field, a dark field, and a fluorescence image of the cell suspension.