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Yellow mushroom standardized component manufacturing method and application of components to treatment of lung cancer

A technology for the production of yellow mushrooms, which is applied in the fields of biochemical equipment and methods, drug combinations, and microorganism determination/inspection, etc., can solve the problem of little research on the chemical composition of yellow mushrooms, and achieves expansion of raw material sources, easy judgment, and technology. simple effect

Inactive Publication Date: 2014-01-08
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researches on chanterelles in the above literatures mainly focus on biotransformation, and there are few studies on the chemical constituents of chanterelles

Method used

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  • Yellow mushroom standardized component manufacturing method and application of components to treatment of lung cancer
  • Yellow mushroom standardized component manufacturing method and application of components to treatment of lung cancer
  • Yellow mushroom standardized component manufacturing method and application of components to treatment of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The preparation method of standardized components of chanterelles comprises the following steps:

[0034] ⑴After drying and crushing the chanterelles, extract 3 times with analytical pure water at 40°C according to the solid-liquid ratio of 1g:4mL, and centrifuge to collect the fungus residue, which is mixed with acetone at a ratio of 1g:1mL Extract at 10°C for 3 times, each time for 72 hours, and collect the filtrate by centrifugation; the filtrate is concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.03 MPa and a temperature of 30°C to obtain the acetone extract.

[0035] (2) Add 1 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, and pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized component samples of Fr1, Fr2, Fr3, Fr4, Fr5,...

Embodiment 2

[0046] Example 2 The preparation method of standardized components of chanterelles comprises the following steps:

[0047] ⑴After drying and crushing the chanterelle mushrooms, extract them with analytically pure water at 90°C for 3 times at a solid-liquid ratio of 1g:20 mL, each time for 1 hour, and collect the fungus residue by centrifugation. The ratio of solid to liquid was extracted at 80°C for 3 times, each time for 5 hours, and the filtrate was collected by centrifugation; the filtrate was concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.1 MPa and a temperature of 80°C to obtain the acetone extract.

[0048] (2) Add 6 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, and pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized component samples ...

Embodiment 3

[0052] Example 3 The preparation method of standardized components of chanterelles comprises the following steps:

[0053] After drying and crushing the chanterelles, extract them with analytically pure water at 65°C for 3 times according to the solid-liquid ratio of 1g:12mL, and centrifuge to collect the fungus residue, which is mixed with acetone at the ratio of 1g:10mL. The liquid ratio was extracted at 45°C for 3 times, each time for 38 hours, and the filtrate was collected by centrifugation; the filtrate was concentrated under reduced pressure to a paste under the conditions of a vacuum of 0.06 MPa and a temperature of 55°C to obtain the acetone extract.

[0054] (2) Add 3.5 times its volume of n-hexane-ethyl acetate mixture to the acetone extract to dissolve, pass through a 0.45 μm organic filter to obtain a sample solution containing acetone extract; 10μm silica gel is used as the separation matrix to prepare chromatographic separation to obtain 14 standardized co...

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Abstract

The invention relates to a yellow mushroom standardized component manufacturing method. The method comprises the following steps: (1) drying and crushing yellow mushrooms, then performing water extraction, centrifugally collecting mushroom residues, extracting the mushroom residues with acetone, centrifugally collecting filtrate, and performing reduced pressure concentration on the filtrate to form paste, namely an acetone extract; (2) dissolving the acetone extract in an n-hexane-ethyl acetate mixed solution, then filtering to obtain a sample solution containing the acetone extract, and separating the sample solution containing the acetone extract on a preparation chromatogram to obtain 14 standardized component samples, namely Fr1, Fr2,..., Fr13 and Fr14; and (3) performing anti-lung cancer cell model screening on the 14 standardized component samples in vitro by an iCelligence real-time label-free cell analyzer, thus determining that the 7th, 8th and 14th standardized components have human lung cancer cell adherence and proliferation inhibition activity in vitro. The yellow mushroom standardized component manufacturing method provided by the invention has the advantages of simple process and easiness in implementation. Simultaneously, the obtained active components can be applied to treatment of lung cancer.

Description

Technical field [0001] The present invention involves the field of pharmaceutical chemistry and biomedical technology, especially the Yellow Mushroom standardization component method and its application in lung cancer treatment. Background technique [0002] Lung cancer is the most common primary malignant tumor and one of the most common malignant tumors in the world today.According to statistics from the World Health Organization / International Cancer Research Center, lung cancer accounts for about 19%of all malignant tumors, and is located at the leadership of various malignant tumors. The world increases more than 1.2 million new lung cancer cases each year, and increases at a rate of about 3%.Lung cancer pose a great threat to human survival and health.At present, the research on the early diagnosis of lung cancer, targeted medication system, and anti -cancer Chinese medicine has become the focus of global attention. [0003] Natural drugs have a long history of the treatment...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P35/00C12Q1/02
Inventor 王启兰党军陶燕铎梅丽娟史强强顾朋嫒马琳李园园
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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