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Agent for blood cell analyzer

A technology for blood cell analyzers and reagents, applied in biological testing, material inspection products, etc., can solve the problems of high reagent cost and long supply cycle.

Active Publication Date: 2015-06-10
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the defects of high cost and long supply cycle of the original reagents of the five-differentiation cell analyzer in the prior art, the purpose of the present invention is to independently develop a reagent suitable for the five-differentiation automatic blood cell analyzer with low cost and superior reagent performance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, reagents for hematology analyzer of the present invention

[0055] (1) Prescription of diluent:

[0056] Potassium dihydrogen phosphate 1.0g / L

[0057] Disodium hydrogen phosphate 5.0g / L

[0058] Sodium sulfate 2.5g / L

[0059] Sodium chloride 3.5g / L

[0060] Proclin 300 0.1g / L

[0061] EDTA-2K 0.4g / L

[0062] Add potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate, sodium chloride, and EDTA-2K into a 1000mL beaker according to the above prescription, heat and stir with an appropriate amount of deionized water to dissolve, and add Proclin 300 after the temperature drops to room temperature. The remaining water was added and stirred, and the pH value of the measured solution was 6.5, the conductivity was 15.0 ms / cm, and the osmotic pressure was 270 mOsm / kg, and the dilution was obtained after filtration;

[0063] (2) Prescription of EOI hemolytic agent:

[0064] Tetradecyltrimethylammonium chloride 1.0g / L

[0065] HEPES 4.0g...

Embodiment 2

[0093] Embodiment 2, reagents for hematology analyzer of the present invention

[0094] (1) Prescription of diluent:

[0095] Potassium dihydrogen phosphate 1.36g / L

[0096] Disodium hydrogen phosphate 5.36g / L

[0097] Sodium sulfate 4.63g / L

[0098] Sodium chloride 4.95g / L

[0099] Proclin 300 0.3g / L

[0100] EDTA-2K 0.5g / L

[0101] Add potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate, sodium chloride, and EDTA-2K into a 1000mL beaker according to the above prescription, heat and stir with an appropriate amount of deionized water to dissolve, and add Proclin 300 after the temperature drops to room temperature. The remaining water was added and stirred, and the pH value of the measured solution was 6.0, the conductivity was 18.0 ms / cm, and the osmotic pressure was 300 mOsm / kg, and the diluted solution was obtained after filtration;

[0102] (2) Prescription of EOI hemolytic agent:

[0103] Tetradecyltrimethylammonium chloride 2.0g / L

[0104...

Embodiment 3

[0132] Embodiment 3, reagents for hematology analyzer of the present invention

[0133] (1) Prescription of diluent:

[0134] Potassium dihydrogen phosphate 4.0g / L

[0135] Disodium hydrogen phosphate 6.0g / L

[0136] Sodium sulfate 5.5g / L

[0137] Sodium chloride 2.0g / L

[0138] Proclin 300 0.5g / L

[0139] EDTA-2K 0.3g / L

[0140] Add potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate, sodium chloride, and EDTA-2K into a 1000mL beaker according to the above prescription, heat and stir with an appropriate amount of deionized water to dissolve, and add Proclin 300 after the temperature drops to room temperature. The remaining water was added and stirred, and the pH value of the measured solution was 7.0, the conductivity was 20.0 ms / cm, and the osmotic pressure was 320 mOsm / kg, and the diluted solution was obtained after filtration;

[0141] (2) Prescription of EOI hemolytic agent:

[0142] Tetradecyltrimethylammonium chloride 4.0g / L

[0143] HEP...

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Abstract

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100 / 5180 / 5300 / 5380 / 5310 / 5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

Description

technical field [0001] The invention belongs to reagents for medical diagnostic instruments, in particular to a reagent for blood cell analyzers. Background technique [0002] Traditional hematological routine inspection methods are artificial blood red blood cells with the help of microscopes, white blood cell technology and blood smear staining microscopes for naked eye artificial white blood cell classification. This operation is inefficient, time-consuming and laborious, and does not meet the needs of the market. With the development of science and technology, the above situation has been changed since the advent of hematology analyzer. [0003] With the rapid development of science and technology, blood cell analysis technology has made great progress, and can accurately provide 27 detection parameters, providing a more accurate and effective method for clinical examination. The blood cell five-category technology is the most advanced analysis technology at present. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 何林辉张志花黄伟豪
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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