The invention discloses a blood visible component detection microscopic examination sample single-step
dyeing preparation method. The method comprises the following steps: T10, collecting a blood sample of a detection object by using a
blood collection tube; t20, taking the volume VT of the blood sample by using a
pipette, and adding the blood sample into a
reagent tube filled with a
dilution staining reagent to obtain a microscopic examination sample, wherein the volume of the
dilution staining reagent in the reagent tube is VA; diluting the blood sample by using the
dilution staining reagent; t30, taking a microscopic examination sample with the volume equal to that of VB by using a
pipette, and adding the microscopic examination sample into a sample adding opening of the blood visible component detection
chip at one time; the dilution staining reagent comprises a staining agent, and the staining agent is used for staining cells in the blood sample; the dilution staining reagent comprises a protective agent, and the protective agent acts on a
cell membrane in a blood sample, so that the
cell membrane is stable and is not easy to break. The operation steps and the volume relation of various turbid liquid components added in each link are defined in detail, so that the preparation of the microscopic examination sample is more efficient, and the subsequent high-precision analysis and identification can be supported.