15 results about "Cell–cell interaction" patented technology

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

An object of the present invention is to provide a method of evaluating intercellular interactions in neuroinflammation using a co-culture containing human-derived neural cells capable of mimicking human brain functions. According to the present invention, there is provided a method of evaluating intercellular interactions in neuroinflammation, the method including a step of producing a co-culture containing at least two cells selected from the group consisting of human-derived astrocytes, human-derived neurons, human-derived microglia, and human-derived oligodendrocytes, a step of applying an inflammatory stimulation to a first cell contained in the co-culture, a step of detecting at least one selected from the group consisting of an inflammatory response marker in the cells contained in the co-culture, neural activity of the cells, and cell morphology, and a step of evaluating, over time, a change in at least one selected from the group consisting of the inflammatory response marker, the neural activity, and the cell morphology in the first cell and a second cell different from the first cell contained in the co-culture.

The application provides a cell array capture and pairing microfluidic chip, which comprises cell pair units arranged in an array, each cell pair unit being provided with a large micro-trap, three small micro-traps arranged in a sinking interval in the large micro-trap, three groups of electrode pairs and shielding electrodes between adjacent small micro-traps, and the small micro-traps being located between corresponding electrode pairs. The cell capture and pairing microfluidic chip can capture three kinds of cells on the chip in sequence, realize large-scale triple cell array, and arrange the three kinds of cells in the array in one of each kind of cell and three cells as a group for pairing, so that great convenience and possibility are provided for studying three-cell paracrine and other intercellular interactions or cell fusion.

The application discloses a platelet detection kit based on multi-parameter flow cytometry and a use method thereof, relates to the technical field of in-vitro diagnosis, and covers the core pathological links of thrombosis and downstream key events of thrombosis by simultaneously detecting the proportion of PNH clone cells, the platelet activation state and the proportion of platelet-leukocyte aggregates (PLAs), thereby efficiently and accurately evaluating the thrombosis risk of PNH patients. The application directly detects cell surface markers and cell-cell interaction by using multi-color flow cytometry, can early and sensitively identify abnormal activation signals, and avoids the limitations of traditional coagulation indexes (such as D-dimer) in PNH thrombosis early warning.

The invention discloses an intelligent evaluation method and system for an immunohistochemical staining result, and relates to the technical field of immunohistochemical staining, and the method comprises the steps of data generation, cell region data generation, classification data generation, spatial analysis result data generation and adaptive scoring and quality control. The consistency and comparability of data are guaranteed, and deviation caused by dyeing difference and image quality fluctuation is overcome; secondly, accurately identifying each cell region by a cell nucleus instance segmentation technology, and providing detailed region attributes; further through multi-modal spatial analysis, the spatial distribution mode of protein expression and the interaction between cells are expressed, and more comprehensive data are provided for pathological mechanism research; and the reliability of the analysis process and the credibility of the result are ensured by a self-adaptive scoring and quality control mechanism, so that the accuracy, efficiency and interpretability of immunohistochemical image analysis are remarkably improved.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The current invention relates to cell-cell interaction and in particular to cellular avidity. Provided are improved means and methods to study cell-cell interaction and characterizing cellular avidity. Highly advantageously, it was shown that cellular avidity methods allowed to enrich for B cells expressing an antigen-specific antibody from B cells obtained from subjects exposed to said antigen, e.g. via infection, vaccination, or immunization. Hence, improved means and methods for use in antibody discovery and development are provided. Highly advantageously, it was also shown that cellular avidity methods allowed to determine immune responses against antigens from B cells obtained from subjects exposed to said antigen, e.g. via infection, vaccination, or immunization. Hence, improved means and methods for determining immune response are also provided.

The present disclosure relates to integrated microfluidic platforms and systems that automate cell¬ based assays, including cell-cell interaction assays, with applications in precision medicine. Requiring only a minimal number of cells, the disclosed devices and systems enable the customization of treatment options by evaluating the functional responses of a patient's cells to a defined drug panel. This approach preserves the heterogeneity of the original sample, thereby enhancing the accuracy of personalized treatment regimens.

Provided are a single-cell transcriptome data analysis and processing method and an electronic device. The method comprises: acquiring a preprocessed first data set, and determining an intercellular interaction intensity and ligand-receptor difference pairs from the first data set by means of a CellPhoneDB tool, a CellChat tool and a Cellcall tool, so as to obtain a communication analysis result representing intercellular communication; upon receiving a second instruction for cell type annotation, calling from a tool library preset dimensionality reduction clustering tools to perform dimensionality reduction clustering on the first data set, so as to obtain a clustered second data set; calling an annotation tool from the tool library to perform cell type annotation on the second data set, so as to obtain an annotation result; and, on the basis of a preset visualization strategy, visually displaying the communication analysis result and the annotation result. Integrating the processing operations such as cell communication analysis, dimensionality reduction clustering and cell annotation helps to improve the flexibility of data analysis, thereby further improving the efficiency of data analysis.

The present application is suitable for the field of biological detection and cosmetic safety evaluation technology, and provides a cosmetic raw material toxicity evaluation method based on co-culture of hair follicle stem cells and endothelial cells, which comprises the following steps: separating hair follicle stem cells and endothelial cells from mammalian skin tissue; establishing a non-contact co-culture system of hair follicle stem cells and endothelial cells by using a Transwell chamber, so as to simulate the intercellular interaction in the microenvironment of hair follicle stem cells; applying the cosmetic raw material to be tested to the co-culture system at a gradient concentration; detecting six indexes in six dimensions, i.e., cell viability, cell apoptosis, stem cell marker expression, endothelial barrier function, inflammatory factor release and genetic damage; calculating a comprehensive toxicity score according to a weighted comprehensive toxicity index model and determining a toxicity grade. The present application significantly improves the sensitivity and physiological relevance of toxicity detection, and provides a high-throughput, multi-index and standardized in-vitro alternative method for safety evaluation of cosmetic raw materials.

A biological tissue forming device that ensures a cell-cell interaction and an exchange of liquid components between cell layers of a formed biological tissues with high efficiency can be provided. A biological tissue forming device for forming a biological tissue having a plurality of cell layers formed of adherent cells has both surfaces on which culture regions of the adherent cells are disposed, and includes a culture membrane arranged between the plurality of cell layers after the adherent cells are cultured and a plurality of flow passages divided by the culture membrane. The culture membrane is formed of a readily-soluble material.

The invention discloses a construction method and application of a matrigel suppository telocyte immortalized cell line, which comprises the following steps: recruiting in vivo matrigel suppository, sorting telocytes by CD34 immunomagnetic beads, and combining SV40T transduction to establish the telocyte immortalized cell line. And carrying out morphological observation, specific marker detection and functional verification to identify that the obtained cell line is the teterocyte. According to the construction method, the teterocyte closer to the in-vivo functional state can be efficiently obtained, and the obtained cell line can be stably passaged for a long time and maintain the morphological characteristics and biological functions of primary cells; a stable and reliable tool is provided for in-vitro research such as subcutaneous microenvironment research, drug screening and cell therapy, tissue engineering and regenerative medicine and continuous in-depth research in related fields, and the method has wide application value in multiple scientific research fields such as tissue engineering model construction, intercellular interaction research and drug effect evaluation.

The present invention addresses the problem of providing a method for evaluating intercellular interaction in neuroinflammation using a co-culture containing human nervous system cells capable of mimicking human brain functions. According to the present invention, provided is a method for evaluating the intercellular interaction of neuroinflammation, the method comprising: a step for producing a co-culture comprising at least two types of cells selected from the group consisting of human astrocytes, human nerve cells, human microglial cells, and human oligodendrocytes; a step for applying an inflammatory stimulus to a first cell included in the co-culture; a step for detecting at least one substance selected from the group consisting of intracellular inflammatory response markers, cellular neural activity, and cellular morphology included in the co-culture; and a step for evaluating, over time, a change in at least one type selected from the group consisting of the inflammatory response marker, the neural activity, and the cellular morphology in the first cell and a second cell different from the first cell included in the co-culture.

The application discloses a chemical labeling system HIM-IP for detecting cell-cell interaction. The system is constructed by modifying tyrosinase and phenol on the surfaces of two cells to be detected respectively, constructing a trapper cell and a prey cell, and then using biotin or single-stranded DNA with a nucleophilic group as a molecular tag for labeling. The system can not only be detected by traditional flow cytometry, but also be compatible with commercial single-cell transcriptome sequencing platforms, realizing the correlation of cell-cell interaction information and transcriptome information at the single-cell level. The system does not need complex genetic engineering modification of cells, the whole modification and labeling process is simple and efficient, and the detection sensitivity of cell-cell interaction is high. By integrating single-cell sequencing technology, the biological mechanism of cell-cell interaction regulation can be further excavated.