60 results about "Cell–cell interaction" patented technology

The invention discloses a method for establishing a co-culture system of porcine skeletal muscle satellite cells (PSCs) and porcine kidney fibroblasts (PKFs). The method comprises the following steps of: performing in-vitro induction on the PKFs to transdifferentiate into adipocytes, optimizing a process of the method, establishing an optimal PSCs and PKFs co-culture induced differentiation method, and selecting a co-culture proportion. According to the method for in-vitro induction of PKFs transdifferentiation and the co-culture system of PKFs and PSCs established by the invention, the PKFs can replace porcine precursor adipocytes (ADSCs) to serve as a source of seed cells in artificial meat fat, and the period of adipogenesis induced differentiation is shortened; the established PKFs and PSCs co-culture system provides a new two-dimensional model for the artificial snowflake meat; a powerful in-vitro model is provided for deep understanding of intercellular interaction and endocrine and paracrine functions of the skeletal muscle which is a highly heterogeneous tissue.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

The invention discloses a chemical labeling system (HIM-IP) for detecting interaction between cells. According to the system, tyrosinase and phenol are respectively modified on the surfaces of two to-be-detected cells to construct capturing cells and prey cells, and then biotin or single-stranded DNA with nucleophilic groups is used as a molecular tag for labeling. The system not only can perform detection through traditional flow cytometry, but also can be compatible with a commercialized single cell transcriptome sequencing platform to realize association of intercellular interaction information and transcriptome information on a single cell level. The system does not need to carry out complex gene engineering modification on cells, the whole modification and marking process is simple, convenient and efficient, the detection sensitivity of intercellular interaction is high, and the biological mechanism of intercellular interaction regulation and control can be deeply excavated by integrating the single cell sequencing technology.

The invention belongs to the field of biological tissue and organ 3D printing, and discloses a biomimetic valve type organ obtained through 3D biological printing and corresponding 3D printing ink, and the 3D printing ink for forming the biomimetic valve type organ is biological ink obtained by dispersing a photocurable biological material in a phosphate buffer solution, then adding a photoinitiator solution, uniformly mixing, discharging bubbles, and then adding cell spheres to obtain the photoinitiator. The cell ball is a valve interstitial cell ball with a micron-scale diameter, which is obtained by culturing valve interstitial cells by using an agarose mold in the presence of a cell culture medium; and the agarose mold is provided with micron-scale holes. According to the method, the loading mode of the valvular interstitial cells is improved, the cell balls serve as the minimum printing unit, damage to the cells in the 3D printing period is avoided, the interaction between the cells is enhanced, the cells are stimulated to secrete the extracellular matrix, and extracellular matrix remodeling is completed.

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention discloses a vascularized skin chip, a vascularized skin model and application of the vascularized skin chip and the vascularized skin model. The material of the vascularized skin chip does not contain polydimethylsiloxane; the vascularized skin chip comprises a dermal culture chip layer and an epidermal culture chip layer from bottom to top, wherein a vertically through culture cavity is formed between the dermal culture chip layer and the epidermal culture chip layer; the dermal culture chip layer is provided with a capillary channel, and the capillary channel is used for constructing a capillary in the dermal layer. According to the vascularized skin chip, dermis and epidermis can make direct contact, interaction between cells is truly simulated, the activity and functions of the cells are kept, good mechanical support is provided for the epidermis and the dermis layer, and cell growth and tissue form maturation are promoted. The microvessel channel can simulate microvessels of a corium layer and is used for simulating supply of nutrient substances and excretion of metabolic waste, a dynamic fluid environment is provided, and the skin structure and the microenvironment are restored more truly.

The invention belongs to the technical field of medical treatment, and particularly relates to a cancer prognosis marker screening method which comprises the following steps: step 1) acquiring an imaging mass spectrometry flow cytometry analysis data set; 2) performing single cell segmentation on the image in the imaging mass spectrometry flow cytometry analysis data set, and annotating the cell type of the single cell according to the expression intensity of the marker to be screened; 3) identifying an area where the epithelial cells, the immune cells and the fibrotic cells intersect, and obtaining marker expression intensity in the area where the epithelial cells, the immune cells and the fibrotic cells intersect; 4) obtaining an intercellular interaction analysis result based on the cell frequency and the marker expression intensity, and 5) performing regression analysis according to the cell frequency, the marker expression intensity and the intercellular interaction result in different comparison groups, and screening to obtain the prognostic marker.

The present application relates to a kind of intercellular interaction reconstruction method and system based on graph convolution and long short-term memory attention network.The method comprises the following steps: collecting spatial transcriptome data under single cell or subcellular resolution, obtaining the gene expression matrix corresponding to each cell;Determine the spatial proximity between each cell, determine whether there is interaction between cells, thereby constructing cell graph;Using graph convolution and long short-term memory attention network combination, form feature extraction network to extract features from cell graph, obtain latent feature representation;Using inner product to decode latent feature representation, generate new adjacency matrix, obtain the intercellular interaction after reconstruction.By introducing the combination of graph convolution and long short-term memory attention network, the spatial correlation in cell graph structure can be captured while learning intercellular interaction, and the intercellular interaction is reconstructed, so that the intercellular interaction and the organization homeostasis of organism can be better understood.

The present invention discloses a biomimetic vascularized iPSC-hepatocyte sphere for liver regeneration and a preparation method thereof. The biomimetic vascularized iPSC-hepatocyte sphere is a microcapsule with porous methacryloylated hyaluronic acid as an outer shell and human induced pluripotent stem cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells are adhered to the surface of the outer shell. The biomimetic vascularized iPSC-hepatocyte sphere of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for the encapsulated hiPSC-hep sphere to avoid the attack of the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell sphere improves the communication between cells, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair the acute failure liver.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

A biological tissue formation device capable of efficiently performing cell-to-cell interaction and liquid component exchange between cell layers of a formed biological tissue is provided. A biological tissue formation device (1) is provided for forming a biological tissue having multiple cell layers composed of adherent cells (30, 31), comprising: a culture membrane (10) having culture areas for adherent cells (30, 31) on both sides, wherein the cultured adherent cells (30, 31) are placed between the multiple cell layers, and multiple flow paths (110, 130) are divided by the culture membrane (10); the culture membrane (10) is composed of a soluble material (101).

An object of the present invention is to provide a method of evaluating intercellular interactions in neuroinflammation using a co-culture containing human-derived neural cells capable of mimicking human brain functions. According to the present invention, there is provided a method of evaluating intercellular interactions in neuroinflammation, the method including a step of producing a co-culture containing at least two cells selected from the group consisting of human-derived astrocytes, human-derived neurons, human-derived microglia, and human-derived oligodendrocytes, a step of applying an inflammatory stimulation to a first cell contained in the co-culture, a step of detecting at least one selected from the group consisting of an inflammatory response marker in the cells contained in the co-culture, neural activity of the cells, and cell morphology, and a step of evaluating, over time, a change in at least one selected from the group consisting of the inflammatory response marker, the neural activity, and the cell morphology in the first cell and a second cell different from the first cell contained in the co-culture.

The present invention provides a cell composition for treating detrusor underactivity and a method for preparing the same. The cell composition comprises a cell spheroid formed by seeding a plurality of mesenchymal stem cells in a culture device comprising a plurality of culture wells, wherein each of the culture wells has a surface coated with a low-protein-adhesion layer, and culturing the cells under the conditions to induce spheroid formation. Through the cell composition of the present invention, three-dimensional structured cell spheroids are formed to promote intercellular interactions and physiological function expression, thereby enhancing cell survival rates and functional performance after transplantation. In particular, the cell composition effectively facilitates the repair and restoration of contractile function of bladder muscle tissue, thereby improving the clinical symptoms associated with detrusor underactivity.

Described herein are systems and methods for analyzing intracellular interactions including a method, comprising: a) forming a plurality of compartments within a fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments; and detecting the intercellular interaction between the first cell and a second cell of the plurality of cells.

This invention belongs to the field of biological detection, specifically relating to a method for constructing a dynamic proteome interaction network, and more specifically, to a method for constructing a dynamic proteome interaction network of secretory proteins and membrane proteins. Using the method of this invention, a dynamic intercellular interaction network encompassing time, space, and activation can be constructed, thereby revealing the characteristics and dynamic changes of protein-mediated intercellular interactions in the microenvironment. This provides an important foundation for further research on diseases involved in the samples, such as tumor development, treatment strategies, and prognosis.

The application provides a cell array capture and pairing microfluidic chip, which comprises cell pair units arranged in an array, each cell pair unit being provided with a large micro-trap, three small micro-traps arranged in a sinking interval in the large micro-trap, three groups of electrode pairs and shielding electrodes between adjacent small micro-traps, and the small micro-traps being located between corresponding electrode pairs. The cell capture and pairing microfluidic chip can capture three kinds of cells on the chip in sequence, realize large-scale triple cell array, and arrange the three kinds of cells in the array in one of each kind of cell and three cells as a group for pairing, so that great convenience and possibility are provided for studying three-cell paracrine and other intercellular interactions or cell fusion.

The present invention discloses phenothiazine / phenoxazine-based organic compounds, their preparation methods, and applications. The phenothiazine / phenoxazine-based organic compounds of the present invention have good photophysical properties, high biocompatibility, small molecular weight, large Stokes shift, and excellent targeting performance. They can accurately stain subcellular structures in biological cells and effectively locate them in organelles in a wash-free manner, thereby achieving subcellular structure images with a high signal-to-noise ratio. The phenothiazine / phenoxazine-based organic compounds of the present invention have low cytotoxicity and large Stokes shift as small molecule fluorescent dyes. Compared with commercial organelle dyes, the fluorescent dyes of the present invention can specifically accumulate in cell mitochondria, cell membranes, lysosomes, endoplasmic reticulum, and lipid particles, and have excellent photostability, and can be used to observe the microstructure of cells and cell-to-cell interactions for a long time.

The present disclosure provides a method for quantitatively measuring the adhesion force between bacteria and cells based on fluid force microscopy, comprising: culturing bacteria and cells separately, adding bacteria to the cells to allow the bacteria and cells to interact; selecting a fluid force microscopy probe and pre-treating the probe; searching for a single live bacterium to be captured in a liquid environment where the bacteria and cells interact, applying negative pressure using a fluid force microscopy pressure controller to cause the pinhole at the probe tip to absorb the bacteria, thereby obtaining a fluid force microscopy probe that captures a single bacterium; bringing the fluid force microscopy probe that captures a single bacterium close to the cell surface at a preset speed, staying there for a preset time when a preset force is reached, and then returning to the probe to obtain adhesion force curve data between the bacteria and cells. Utilizing the present disclosure, the degree of interaction between a single bacterium and a cell can be accurately characterized, and the adhesion force between a single bacterium and a cell can be efficiently and quantitatively measured.

A method for detecting intercellular interaction, the method comprising: (1) preparing a first cell expressing a TCR activation response element and a reporter molecule, the first cell expressing TCR; (2) incubating the antigen peptide and a second cell expressing MHC to obtain a second cell loaded with the antigen peptide; (3) marking the first cell by using a first tag to obtain a first cell carrying the tag, and marking the second cell loaded with the antigen peptide by using a second tag to obtain a second cell carrying the tag; (4) wrapping the first cells carrying the labels, the second cells carrying the labels and the magnetic beads with an oil phase to prepare a plurality of liquid drops; (5) injecting a cell lysis solution into the single liquid drop, and sequencing the nucleic acid after lysis; and (6) determining whether the first cell and the second cell in the single liquid drop interact or not according to a sequencing result.

The invention discloses a construction method of a three-dimensional liver micro-tissue model and hepatotoxicity assessment application of the three-dimensional liver micro-tissue model, and belongs to the technical field of environmental science and engineering. The 3D liver micro-tissue model constructed by the invention consists of three cell lines, namely HepaRG (parenchymal hepatic cells), THP-1 (kupffer cells) and hTERT-HSC (hepatic stellate cells), so that a real liver environment can be better simulated, and the defects of a traditional model are overcome; particularly, the occurrence of hepatic fibrosis relates to complex interaction among a plurality of cell lines, however, most of the previous models for evaluating the potential of hepatic fibrosis by chemical substances are lack of consideration of interaction among multiple types of cells no matter whether hepatic parenchymal cell lines or hepatic stellate cell lines are used, and the 3D hepatic micro-tissue model takes the factor into account, so that the potential of hepatic fibrosis cannot be evaluated. Key hepatic fibrosis events caused by hepatic fibrosis substances can be copied, and a more suitable test platform is provided for evaluating the potential of chemical substance hepatic fibrosis.

Inventors have synthesized peptides allowing an engagement of intact CD31 molecules on all the healthy endothelial cells and resting blood platelets and leukocytes that can enter in contact with an implanted device. Those cells can therefore receive the “leave-me-alone” signal delivered by the trans-homophilic engagement of CD31, which is essential to maintain the homeostasis in the circulation and vascularized tissues. Thrombotic or life-threatening occurrence of hemorrhagic or thromboembolic complications have impaired the use of endovascular devices. The devices bearing the mimicking peptides of the present invention are rapidly integrated, because they are perceived by blood platelets and leukocytes as a healthy endothelium, a “self” component. Furthermore, their ability to be rapidly endothelialized with a physiologic endothelial cell phenotype also limits platelet and leukocyte activation at the site of device implantation in the long-term. Accordingly, the present invention relates to peptides mimicking the trans-homophilic CD31-CD31 domain 1 and 2 intercellular interaction.

Provided are a cell-containing hydrogel body and a method of producing the same, which enable simple and effective control of the size of a boundary surface for an interaction between cells. The method of producing a cell-containing hydrogel body includes: forming, under a gas phase, a first hydrogel droplet on a surface of a substrate, the first hydrogel droplet containing first cells being dispersed therein and a first hydrogel polymer; forming, under a gas phase, a second hydrogel droplet on the surface, the second hydrogel droplet containing second cells being dispersed therein and a second hydrogel polymer, the second hydrogel droplet being combined with the first hydrogel droplet; and forming, under a gas phase, a cell-containing hydrogel body on the surface by gelling a hydrogel droplet-combined body including a first droplet portion derived from the first hydrogel droplet and a second droplet portion derived from the second hydrogel droplet.

To provide methods of determining the propensity of cells to interact in a population of cells comprising at least two distinguishable subpopulations of cells, and to provide methods of diagnosing a disease or predisposition to a disease of a cell donor.SOLUTION: A method comprises determining a propensity of cells to interact in a population of cells obtained from a donor, where the population of cells comprises at least two distinguishable subpopulations of cells. The invention also provides methods of determining whether or not a subject suffering from or predisposed to a disease will respond or is responsive to treatment with a therapeutic agent by determining the alteration of the propensity of cells to interact in a population of cells obtained from the subject, where the population of cells comprises at least two distinguishable subpopulations of cells. The invention also relates to methods of screening for a therapeutic agent, comprising determining whether one or more test substances alter the propensity of cells to interact.SELECTED DRAWING: None

The application discloses a platelet detection kit based on multi-parameter flow cytometry and a use method thereof, relates to the technical field of in-vitro diagnosis, and covers the core pathological links of thrombosis and downstream key events of thrombosis by simultaneously detecting the proportion of PNH clone cells, the platelet activation state and the proportion of platelet-leukocyte aggregates (PLAs), thereby efficiently and accurately evaluating the thrombosis risk of PNH patients. The application directly detects cell surface markers and cell-cell interaction by using multi-color flow cytometry, can early and sensitively identify abnormal activation signals, and avoids the limitations of traditional coagulation indexes (such as D-dimer) in PNH thrombosis early warning.

Disclosed herein are matrix-free and in vivo-like glioblastoma organoids (GBOs) developed using patient-derived xenograft GBM lines in small-scale bioreactors and a method of scalable biomanufacture thereof. Shear stress, agitation rate, and media supplements can be optimized to produce highly reproducible GBOs over 1 mm diameter within 4-5 weeks. GBOs can exhibit high stemness and strong cell-to-cell interactions compared to conventional tumorsphere cultures. They can display spatial gradients of HIF-1α positive hypoxic cores where CD133-positive cells resided and spatially heterogeneous expression of NOTCH and its ligands. A self-established, hierarchically organized, and heterogeneous TME by GBM transdifferentiation into endothelial cells, pericytes, and astrocytes can also be observed. Collectively, the ability to biomanufacture uniformly sized GBOs that recapitulate in vivo GBM TME features that can serve as an improved GBM in vitro model is demonstrated herein.

The invention belongs to the field of biological detection, particularly relates to a method for constructing a proteome dynamic interaction network, and more particularly relates to a method for constructing a secretory protein-membrane protein proteome dynamic interaction network. By using the method disclosed by the invention, an intercellular time, space and activation dynamic interaction network can be constructed, so that the characteristics and dynamic change of protein-mediated intercellular interaction in a microenvironment are disclosed, and an important basis is provided for further researching the development, treatment strategy and prognosis of diseases related to samples, such as tumors.

The invention discloses an intelligent evaluation method and system for an immunohistochemical staining result, and relates to the technical field of immunohistochemical staining, and the method comprises the steps of data generation, cell region data generation, classification data generation, spatial analysis result data generation and adaptive scoring and quality control. The consistency and comparability of data are guaranteed, and deviation caused by dyeing difference and image quality fluctuation is overcome; secondly, accurately identifying each cell region by a cell nucleus instance segmentation technology, and providing detailed region attributes; further through multi-modal spatial analysis, the spatial distribution mode of protein expression and the interaction between cells are expressed, and more comprehensive data are provided for pathological mechanism research; and the reliability of the analysis process and the credibility of the result are ensured by a self-adaptive scoring and quality control mechanism, so that the accuracy, efficiency and interpretability of immunohistochemical image analysis are remarkably improved.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The invention discloses an intercellular interaction quantitative detection method based on a monolayer cell adsorption experiment, which comprises the following steps: constructing an experiment system conforming to a Langmuir model, and respectively taking cells transfected with different fluorescent label interaction proteins as monolayer recipient cells and ligand cells, after incubation, observing under a fluorescence microscope, counting the number of adsorbed cells, and fitting a corresponding formula to obtain a dissociation constant Kd of the interaction between the cells, so as to realize quantitative evaluation on the affinity. The method provides a new visual angle and tool for studying the interaction between cells and understanding the function exertion of the cells and the interaction of membrane proteins on the surfaces of the cells, and solves the problems that the interaction between the cells cannot be effectively and quantitatively evaluated due to the technical limitation of high requirements on equipment and data, and the membrane proteins are low in content and insoluble in water; and a conventional protein purification method is difficult to obtain a high-purity and high-activity membrane protein sample and is difficult to accurately describe the binding affinity between membrane proteins.

Microfluidic devices and methods are provided for single-cell analysis, isolation, and preparation for immunotherapy. The technology allows functional phenotyping by observing cell-cell interactions and then cells with desirable features or probing the underlying biology. A droplet-based microfluidic device is capable of trapping droplets and releasing them selectively using microvalves. Each droplet can encapsulate effector cells, such as natural killer cells (NK cells) and target cells, such as tumor cells, for real-time monitoring of burst kinetics and spatial coordination during killing of tumor cells by individual NK cells. The technology also allows for interrogating interactions and real-time monitoring of kinetics and then recovering live cells on demand for single-cell genomic or proteomic analysis.