31 results about "Cell–cell interaction" patented technology

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The present application relates to a kind of intercellular interaction reconstruction method and system based on graph convolution and long short-term memory attention network.The method comprises the following steps: collecting spatial transcriptome data under single cell or subcellular resolution, obtaining the gene expression matrix corresponding to each cell;Determine the spatial proximity between each cell, determine whether there is interaction between cells, thereby constructing cell graph;Using graph convolution and long short-term memory attention network combination, form feature extraction network to extract features from cell graph, obtain latent feature representation;Using inner product to decode latent feature representation, generate new adjacency matrix, obtain the intercellular interaction after reconstruction.By introducing the combination of graph convolution and long short-term memory attention network, the spatial correlation in cell graph structure can be captured while learning intercellular interaction, and the intercellular interaction is reconstructed, so that the intercellular interaction and the organization homeostasis of organism can be better understood.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

An object of the present invention is to provide a method of evaluating intercellular interactions in neuroinflammation using a co-culture containing human-derived neural cells capable of mimicking human brain functions. According to the present invention, there is provided a method of evaluating intercellular interactions in neuroinflammation, the method including a step of producing a co-culture containing at least two cells selected from the group consisting of human-derived astrocytes, human-derived neurons, human-derived microglia, and human-derived oligodendrocytes, a step of applying an inflammatory stimulation to a first cell contained in the co-culture, a step of detecting at least one selected from the group consisting of an inflammatory response marker in the cells contained in the co-culture, neural activity of the cells, and cell morphology, and a step of evaluating, over time, a change in at least one selected from the group consisting of the inflammatory response marker, the neural activity, and the cell morphology in the first cell and a second cell different from the first cell contained in the co-culture.

The present invention provides a cell composition for treating detrusor underactivity and a method for preparing the same. The cell composition comprises a cell spheroid formed by seeding a plurality of mesenchymal stem cells in a culture device comprising a plurality of culture wells, wherein each of the culture wells has a surface coated with a low-protein-adhesion layer, and culturing the cells under the conditions to induce spheroid formation. Through the cell composition of the present invention, three-dimensional structured cell spheroids are formed to promote intercellular interactions and physiological function expression, thereby enhancing cell survival rates and functional performance after transplantation. In particular, the cell composition effectively facilitates the repair and restoration of contractile function of bladder muscle tissue, thereby improving the clinical symptoms associated with detrusor underactivity.

Described herein are systems and methods for analyzing intracellular interactions including a method, comprising: a) forming a plurality of compartments within a fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments; and detecting the intercellular interaction between the first cell and a second cell of the plurality of cells.

This invention belongs to the field of biological detection, specifically relating to a method for constructing a dynamic proteome interaction network, and more specifically, to a method for constructing a dynamic proteome interaction network of secretory proteins and membrane proteins. Using the method of this invention, a dynamic intercellular interaction network encompassing time, space, and activation can be constructed, thereby revealing the characteristics and dynamic changes of protein-mediated intercellular interactions in the microenvironment. This provides an important foundation for further research on diseases involved in the samples, such as tumor development, treatment strategies, and prognosis.

The application provides a cell array capture and pairing microfluidic chip, which comprises cell pair units arranged in an array, each cell pair unit being provided with a large micro-trap, three small micro-traps arranged in a sinking interval in the large micro-trap, three groups of electrode pairs and shielding electrodes between adjacent small micro-traps, and the small micro-traps being located between corresponding electrode pairs. The cell capture and pairing microfluidic chip can capture three kinds of cells on the chip in sequence, realize large-scale triple cell array, and arrange the three kinds of cells in the array in one of each kind of cell and three cells as a group for pairing, so that great convenience and possibility are provided for studying three-cell paracrine and other intercellular interactions or cell fusion.

The present disclosure provides a method for quantitatively measuring the adhesion force between bacteria and cells based on fluid force microscopy, comprising: culturing bacteria and cells separately, adding bacteria to the cells to allow the bacteria and cells to interact; selecting a fluid force microscopy probe and pre-treating the probe; searching for a single live bacterium to be captured in a liquid environment where the bacteria and cells interact, applying negative pressure using a fluid force microscopy pressure controller to cause the pinhole at the probe tip to absorb the bacteria, thereby obtaining a fluid force microscopy probe that captures a single bacterium; bringing the fluid force microscopy probe that captures a single bacterium close to the cell surface at a preset speed, staying there for a preset time when a preset force is reached, and then returning to the probe to obtain adhesion force curve data between the bacteria and cells. Utilizing the present disclosure, the degree of interaction between a single bacterium and a cell can be accurately characterized, and the adhesion force between a single bacterium and a cell can be efficiently and quantitatively measured.

Inventors have synthesized peptides allowing an engagement of intact CD31 molecules on all the healthy endothelial cells and resting blood platelets and leukocytes that can enter in contact with an implanted device. Those cells can therefore receive the “leave-me-alone” signal delivered by the trans-homophilic engagement of CD31, which is essential to maintain the homeostasis in the circulation and vascularized tissues. Thrombotic or life-threatening occurrence of hemorrhagic or thromboembolic complications have impaired the use of endovascular devices. The devices bearing the mimicking peptides of the present invention are rapidly integrated, because they are perceived by blood platelets and leukocytes as a healthy endothelium, a “self” component. Furthermore, their ability to be rapidly endothelialized with a physiologic endothelial cell phenotype also limits platelet and leukocyte activation at the site of device implantation in the long-term. Accordingly, the present invention relates to peptides mimicking the trans-homophilic CD31-CD31 domain 1 and 2 intercellular interaction.

The application discloses a platelet detection kit based on multi-parameter flow cytometry and a use method thereof, relates to the technical field of in-vitro diagnosis, and covers the core pathological links of thrombosis and downstream key events of thrombosis by simultaneously detecting the proportion of PNH clone cells, the platelet activation state and the proportion of platelet-leukocyte aggregates (PLAs), thereby efficiently and accurately evaluating the thrombosis risk of PNH patients. The application directly detects cell surface markers and cell-cell interaction by using multi-color flow cytometry, can early and sensitively identify abnormal activation signals, and avoids the limitations of traditional coagulation indexes (such as D-dimer) in PNH thrombosis early warning.

The invention discloses an intelligent evaluation method and system for an immunohistochemical staining result, and relates to the technical field of immunohistochemical staining, and the method comprises the steps of data generation, cell region data generation, classification data generation, spatial analysis result data generation and adaptive scoring and quality control. The consistency and comparability of data are guaranteed, and deviation caused by dyeing difference and image quality fluctuation is overcome; secondly, accurately identifying each cell region by a cell nucleus instance segmentation technology, and providing detailed region attributes; further through multi-modal spatial analysis, the spatial distribution mode of protein expression and the interaction between cells are expressed, and more comprehensive data are provided for pathological mechanism research; and the reliability of the analysis process and the credibility of the result are ensured by a self-adaptive scoring and quality control mechanism, so that the accuracy, efficiency and interpretability of immunohistochemical image analysis are remarkably improved.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The current invention relates to cell-cell interaction and in particular to cellular avidity. Provided are improved means and methods to study cell-cell interaction and characterizing cellular avidity. More in particular, the methods involve studying cell-cell interactions and applying forces in order to break cell-cell bonds in a controllable fashion. Analysing such interactions and breakage of cell bonds in time allows to assess cell-cell binding dynamics.

The current invention relates to cell-cell interaction and in particular to cellular avidity. Provided are improved means and methods to study cell-cell interaction and characterizing cellular avidity. Highly advantageously, it was shown that cellular avidity methods allowed to enrich for B cells expressing an antigen-specific antibody from B cells obtained from subjects exposed to said antigen, e.g. via infection, vaccination, or immunization. Hence, improved means and methods for use in antibody discovery and development are provided. Highly advantageously, it was also shown that cellular avidity methods allowed to determine immune responses against antigens from B cells obtained from subjects exposed to said antigen, e.g. via infection, vaccination, or immunization. Hence, improved means and methods for determining immune response are also provided.

Disclosed herein include methods, devices, kits, and systems for nucleic acid sequencing, for example, to determine cell-cell interaction using a dielectrophoresis microfluidic device.

The invention discloses a gold nanocluster composite material functionalized microsphere and application thereof, and belongs to the technical field of biomedicine. According to the invention, the gold nanocluster loaded microRNA is used as a stem cell differentiation inducer, and is introduced into the GelMA / HAMA gel for constructing the functional microspheres (ARGH microgel), so that necessary conditions are provided for the growth and differentiation of stem cells. The ARGH microspheres provide sites for stem cell adhesion and growth, and meanwhile, surface stem cells are regulated and controlled to be differentiated into hepatocytes. A cell-loaded ARGH microsphere secretion group shows angiogenesis promoting and immunoregulation activity, and meanwhile, cells on the surfaces of different ARGH microspheres are aggregated through intercellular interaction and are self-assembled into a micro-tissue with a larger size. The micro liver tissue is transplanted into the liver failure mouse body, the function and the treatment effect of the micro liver tissue are further evaluated, and the result shows that in two mouse liver failure models, micro liver tissue transplantation treatment can effectively recover the liver function and promote liver regeneration and repair.

The present disclosure relates to integrated microfluidic platforms and systems that automate cell¬ based assays, including cell-cell interaction assays, with applications in precision medicine. Requiring only a minimal number of cells, the disclosed devices and systems enable the customization of treatment options by evaluating the functional responses of a patient's cells to a defined drug panel. This approach preserves the heterogeneity of the original sample, thereby enhancing the accuracy of personalized treatment regimens.

Provided are a single-cell transcriptome data analysis and processing method and an electronic device. The method comprises: acquiring a preprocessed first data set, and determining an intercellular interaction intensity and ligand-receptor difference pairs from the first data set by means of a CellPhoneDB tool, a CellChat tool and a Cellcall tool, so as to obtain a communication analysis result representing intercellular communication; upon receiving a second instruction for cell type annotation, calling from a tool library preset dimensionality reduction clustering tools to perform dimensionality reduction clustering on the first data set, so as to obtain a clustered second data set; calling an annotation tool from the tool library to perform cell type annotation on the second data set, so as to obtain an annotation result; and, on the basis of a preset visualization strategy, visually displaying the communication analysis result and the annotation result. Integrating the processing operations such as cell communication analysis, dimensionality reduction clustering and cell annotation helps to improve the flexibility of data analysis, thereby further improving the efficiency of data analysis.

The invention provides a micro-fluidic chip system and a chip manufacturing method thereof. The micro-fluidic chip system comprises a cell culture chip, a DNA logic gate calculation chip and an electrochemical sensing chip. The cell culture chip is used for culturing cells, the DNA logic gate calculation chip is used for calculating a DNA molecular signal, the electrochemical sensing chip is used for converting the DNA molecular signal into a corresponding current signal, and visual output of a logic relation is carried out. According to the application, the intercellular interaction and communication mode which is difficult to directly observe originally can be converted into quantifiable and measurable current signals through DNA logic calculation; the logic relation between the cells is dynamically monitored in real time; and high sensitivity and high specificity can accurately detect the specific cell signal molecule logic relationship.

The application belongs to the technical field of medical treatment and specifically relates to a screening method of a cancer prognosis marker, comprising the following steps: step one) obtaining an imaging mass spectrometry flow cytometry analysis data set; step two) performing single cell segmentation on images in the imaging mass spectrometry flow cytometry analysis data set, and annotating cell types of single cells according to expression intensity of the marker to be screened; step three) identifying an area where epithelial, immune and fibrotic cells intersect, and obtaining marker expression intensity in the area where epithelial, immune and fibrotic cells intersect; step four) obtaining an intercellular interaction analysis result based on cell frequency and marker expression intensity; and step five) performing regression analysis according to cell frequency, marker expression intensity and the intercellular interaction result in different comparison groups, and screening to obtain a prognosis marker.

The present application is suitable for the field of biological detection and cosmetic safety evaluation technology, and provides a cosmetic raw material toxicity evaluation method based on co-culture of hair follicle stem cells and endothelial cells, which comprises the following steps: separating hair follicle stem cells and endothelial cells from mammalian skin tissue; establishing a non-contact co-culture system of hair follicle stem cells and endothelial cells by using a Transwell chamber, so as to simulate the intercellular interaction in the microenvironment of hair follicle stem cells; applying the cosmetic raw material to be tested to the co-culture system at a gradient concentration; detecting six indexes in six dimensions, i.e., cell viability, cell apoptosis, stem cell marker expression, endothelial barrier function, inflammatory factor release and genetic damage; calculating a comprehensive toxicity score according to a weighted comprehensive toxicity index model and determining a toxicity grade. The present application significantly improves the sensitivity and physiological relevance of toxicity detection, and provides a high-throughput, multi-index and standardized in-vitro alternative method for safety evaluation of cosmetic raw materials.

Suspendable shaped particles or nanovials are used to viably sort single cells based on their secreted product at high-throughput using only commonly accessible lab infrastructure. These shaped particles act as a solid support which facilitates cell attachment, templates formation of uniform aqueous compartments which prevent cross-talk between cells, and captures secreted proteins. Using this platform, high-throughput screening of producer cells on relative IgG production, B / plasma cells based on secreted antibody binding to antigen, and T cells based on cytokine secretion is demonstrated using commercially available flow sorters. These shaped particles are easily distributed and used, democratizing access to high-throughput functional cell screening.

The invention relates to a method for capturing interaction information between bacteria and host cancer cells, in particular to a method for capturing interaction information between the bacteria and the host cancer cells through proximity labeling reaction catalyzed by a metal ruthenium complex and application of the method. In order to solve the problems that an existing common means for researching the interaction between bacteria and host cancer cells is complex in operation and a sample needs to be additionally treated, terpyridyl ruthenium succinimide ester is used as a core catalyst to catalyze an initiated proximity labeling reaction to capture the interaction between the bacteria and the host cancer cells, and the detection method is used for detecting the interaction between the bacteria and the host cancer cells. Furthermore, the strength information of the interaction is detected and analyzed. The method provided by the invention has the characteristic of high efficiency, only marks biomolecules in a direct contact area of bacteria and host cancer cells, and can effectively avoid background interference of non-contact free cells or non-specific adsorption cells. In addition, the method is simple and convenient to operate and does not need additional treatment on the sample. The method is applied to the cross technical field of biochemistry and chemical biology.

This invention provides a method and system for characterizing, typing, and identifying cells and multicellular aggregates using their physical information. The method analyzes cellular mechanical force and / or hardness, which is measured under various conditions, such as during cell-to-cell interactions or in response to external substances and factors. This characterization of real-time cellular physical states allows for the rapid, low-cost, and high-throughput typing and identification of various cell types and conditions. The system implementing this method achieves an accuracy rate exceeding 98%, offering a powerful new tool for biotechnology.

The application discloses a research method for tumor cell influence mechanism of monocyte remodeling based on bone marrow inflammatory microenvironment, and relates to the technical field of tumor treatment; and comprises the following steps: S1, providing bone marrow plasma from a classic myeloproliferative neoplasm patient; S2, placing primary CD14+ monocytes from a healthy donor in an in-vitro culture system containing the bone marrow plasma, inducing phenotype remodeling of the monocytes, and obtaining the remodeling monocytes. The application establishes CD163+CD206+ double-positive remodeling monocytes as a new target for MPN treatment; through comparison experiments of direct co-culture and indirect co-culture by Transwell, it is confirmed that the promotion effect of the remodeling monocytes on HEL cell proliferation strictly depends on physical contact between cells, rather than soluble secreted factors; and the application provides a theoretical basis for developing new antibody drugs or small molecule inhibitors for blocking cell-cell interaction.

A biological tissue forming device that ensures a cell-cell interaction and an exchange of liquid components between cell layers of a formed biological tissues with high efficiency can be provided. A biological tissue forming device for forming a biological tissue having a plurality of cell layers formed of adherent cells has both surfaces on which culture regions of the adherent cells are disposed, and includes a culture membrane arranged between the plurality of cell layers after the adherent cells are cultured and a plurality of flow passages divided by the culture membrane. The culture membrane is formed of a readily-soluble material.

The invention discloses a construction method and application of a matrigel suppository telocyte immortalized cell line, which comprises the following steps: recruiting in vivo matrigel suppository, sorting telocytes by CD34 immunomagnetic beads, and combining SV40T transduction to establish the telocyte immortalized cell line. And carrying out morphological observation, specific marker detection and functional verification to identify that the obtained cell line is the teterocyte. According to the construction method, the teterocyte closer to the in-vivo functional state can be efficiently obtained, and the obtained cell line can be stably passaged for a long time and maintain the morphological characteristics and biological functions of primary cells; a stable and reliable tool is provided for in-vitro research such as subcutaneous microenvironment research, drug screening and cell therapy, tissue engineering and regenerative medicine and continuous in-depth research in related fields, and the method has wide application value in multiple scientific research fields such as tissue engineering model construction, intercellular interaction research and drug effect evaluation.

The present invention addresses the problem of providing a method for evaluating intercellular interaction in neuroinflammation using a co-culture containing human nervous system cells capable of mimicking human brain functions. According to the present invention, provided is a method for evaluating the intercellular interaction of neuroinflammation, the method comprising: a step for producing a co-culture comprising at least two types of cells selected from the group consisting of human astrocytes, human nerve cells, human microglial cells, and human oligodendrocytes; a step for applying an inflammatory stimulus to a first cell included in the co-culture; a step for detecting at least one substance selected from the group consisting of intracellular inflammatory response markers, cellular neural activity, and cellular morphology included in the co-culture; and a step for evaluating, over time, a change in at least one type selected from the group consisting of the inflammatory response marker, the neural activity, and the cellular morphology in the first cell and a second cell different from the first cell included in the co-culture.

The application discloses a chemical labeling system HIM-IP for detecting cell-cell interaction. The system is constructed by modifying tyrosinase and phenol on the surfaces of two cells to be detected respectively, constructing a trapper cell and a prey cell, and then using biotin or single-stranded DNA with a nucleophilic group as a molecular tag for labeling. The system can not only be detected by traditional flow cytometry, but also be compatible with commercial single-cell transcriptome sequencing platforms, realizing the correlation of cell-cell interaction information and transcriptome information at the single-cell level. The system does not need complex genetic engineering modification of cells, the whole modification and labeling process is simple and efficient, and the detection sensitivity of cell-cell interaction is high. By integrating single-cell sequencing technology, the biological mechanism of cell-cell interaction regulation can be further excavated.