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Method for modifying probe of atomic force microscope by using magnetic nano-particles

A technology of magnetic nanoparticles and atomic force microscopy, which is applied in the direction of scanning probe technology, scanning probe microscopy, measuring devices, etc., can solve the problems of affecting the test, difficult to observe, and demanding experimental conditions, so as to improve the adhesion rate and adhesion strength, improve modification efficiency, and increase the effect of contact area

Active Publication Date: 2018-08-14
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, commonly used probe modification techniques include coating method, gluing method, spray method, solution deposition method, etc. However, since the radius of curvature of the probe tip is at the nanometer level, it is difficult to observe, and the modification of the tip usually requires the help of large-scale microscopic observation and Operating equipment, demanding experimental conditions
At the same time, the surface area of ​​the probe tip is limited, and the above method cannot guarantee the precise attachment of monodisperse nanoparticles to the tip, which affects the subsequent testing of imaging quality and force curves.
Moreover, due to the limited particle adhesion strength, the use strength and reusability of the probe cannot be guaranteed

Method used

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  • Method for modifying probe of atomic force microscope by using magnetic nano-particles
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  • Method for modifying probe of atomic force microscope by using magnetic nano-particles

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Experimental program
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Effect test

Embodiment 1

[0031] a) Synthesis of particles: micron-sized carbon spheres were prepared using the microemulsion method. Manganese-zinc-ferrite magnetic nanoparticles were prepared using a hydrothermal method.

[0032] b) Preparation of micron-sized carbon sphere-nanoparticle mixed dispersion: Take 2 mg of carbon sphere particles with a size of about 1 μm and add them to 10 mL of methanol, and ultrasonically disperse for 10 min with an ultrasonic cleaning oscillator to ensure that the carbon spheres are evenly dispersed in methanol. Then 1mg of magnetic nanoparticles was added to the above dispersion liquid, and ultrasonic cleaning oscillator was used to disperse again for 30 minutes to ensure that the magnetic nanoparticles and carbon spheres were in full contact and evenly adhered to obtain a uniform mixed dispersion liquid.

[0033] c) Modified probes: prepare V-shaped microcantilever and plate probe, and place them under an optical microscope. The vertical distance between V-shaped mic...

Embodiment 2

[0041]a) Synthesis of particles: Micron-sized carbon spheres were prepared using a solvothermal method. Cobalt ferrite magnetic nanoparticles were prepared using a co-precipitation method.

[0042] b) Preparation of micron-scale carbon sphere-nanoparticle mixed dispersion: 1 mg of carbon sphere particles with a size of about 5 μm was added to 10 mL of acetone, and ultrasonically dispersed for 10 min with an ultrasonic cleaning oscillator to ensure that the carbon spheres were evenly dispersed in acetone. Then 3 mg of magnetic nanoparticles were added to the above dispersion liquid, and the ultrasonic cleaning oscillator was used to ultrasonically disperse for 30 minutes to ensure that the magnetic nanoparticles and carbon spheres were in full contact and evenly attached, and a uniformly dispersed mixed dispersion liquid was obtained.

[0043] c) Modified probes: prepare a V-shaped microcantilever and a flat probe, and place them under an optical microscope. The vertical distan...

Embodiment 3

[0051] a) Synthesis of particles: Micron-sized carbon spheres were prepared using a hydrothermal method. Zinc-cobalt-chromium ferrite magnetic nanoparticles were prepared using a vapor deposition method.

[0052] b) Preparation of micron-scale carbon sphere-nanoparticle mixed dispersion: Take 5 mg of carbon sphere particles with a size of about 0.5 μm and add them to 10 mL of n-hexane, and ultrasonically disperse them with an ultrasonic cleaning oscillator for 10 minutes to ensure that the carbon spheres are evenly dispersed in n-hexane middle. Then 5 mg of magnetic nanoparticles were added to the above dispersion liquid, and the ultrasonic cleaning oscillator was used to ultrasonically disperse for 30 min to ensure that the magnetic nanoparticles were fully in contact with the carbon spheres and evenly adhered to obtain a uniformly dispersed mixed dispersion liquid.

[0053] c) Modified probes: prepare a V-shaped microcantilever and a plate probe, and place them under an opt...

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Abstract

The invention belongs to the atomic force microscope measurement technical field and provides a method for modifying the probe of an atomic force microscope by using magnetic nano-particles. With themethod adopted, the direct test of interaction between nano-particles and cells can be realized. According to the method, nano-scale magnetic particles and micro-scale carbon sphere particles are adopted; the V-shaped micro-cantilever and flat plate probe of the atomic force microscope are placed under the microscope; a particle mixed dispersion liquid is added dropwise, washing, drying and otherprocesses are performed, and therefore, a magnetic nano-particle-modified V-shaped cone-like tip probe can be obtained. The micro-scale carbon spheres are adopted as the carriers of the magnetic nano-particles, and therefore, experimental operation can be simplified, modification efficiency can be improved, a modification effect can be optimized, the direct test of the interaction between the nano-particles and the cells can be realized, and further experimental verification is provided for the uptake of the particles by the cells, adhesion forces between the particles and the cells, the survival ability of the cells and the like.

Description

technical field [0001] The invention belongs to the measurement technical field of atomic force microscopes, and relates to a method for modifying atomic force microscope probes with magnetic nanoparticles. Background technique [0002] Magnetic induction hyperthermia is a physical therapy method that uses magnetic particles to enter cells and generate heat to "scald" cancer cells under the action of an external alternating magnetic field. specialty. The use of nano-scale magnetic particles in magnetic induction hyperthermia can efficiently "burn" cancer cells without damaging normal cells, improving the therapeutic effect. [0003] Studying the interaction force between magnetic nanoparticles and cells will help to further clarify the targeted uptake mechanism of nanoparticles and realize the targeted therapy of cancer cells more efficiently. At present, the interaction between nanoparticles and cells is usually selected by observing properties such as cell adhesion, viab...

Claims

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Application Information

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IPC IPC(8): G01Q60/38
CPCG01Q60/38
Inventor 张伟李姮马建立吴承伟马国军
Owner DALIAN UNIV OF TECH
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