Compositions and methods for cell killing

A cellular, biocidal technology, applied in the directions of botanical equipment and methods, chemicals for biological control, biocides, etc., that can solve the problem of insufficient reduction of bacteria, loss of activity, and no teaching of selective killing of polymer structures. type, etc.

Inactive Publication Date: 2010-06-30
OPLON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vermeiren et al. (2002) demonstrated that incorporation of triclosan into low-density polyethylene resulted in activity in a plate overlay assay, but when the plastic was combined with vacuum packaging and frozen storage, bacteria on the surface of the meat not sufficiently reduced
Possible interaction of triclosan with fat components of meat products may result in loss of this activity
Furthermore, none of the aforementioned US patent applications teach configurations of polymers that selectively kill certain cell types

Method used

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  • Compositions and methods for cell killing
  • Compositions and methods for cell killing
  • Compositions and methods for cell killing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Comparison of Bacterial Production (E. coli) in TSB in NafionTM-Coated Vials vs. Uncoated Vials

[0144] Materials and methods

[0145] A 15 ml vial was coated with a commercial solution of Nafion™ (available from Du Pont) and allowed to dry. This resulted in the formation of a thin layer (approximately 50 microns) of polymerized Nafion™ on the inner surface of the vial.

[0146] Coated and uncoated vials were filled with 10 ml TSB and inoculated with E. coli (3×10 6 cfu / ml). The vials were then incubated statically at 30°C. At 0 hours, 3 hours and 3 days after inoculation, samples were taken from the bacterial broth and spread on TSA plates and incubated at 30°C for 24 hours for bacterial counts (cfu / ml).

[0147] result

[0148] figure 1 Represents the bacterial counts of E. coli in Nafion™-coated vs. uncoated vials; figure 2 A comparison of bacterial deposition in uncoated vials (left) and coated vials (right) is shown.

[0149] In uncoated controls, ba...

Embodiment 2

[0152] via Dormin TM Bacteria production in coated vs. uncoated vials

[0153] Dormenin, a natural extract from plants and plant organs in their dormant phase, slows cell proliferation, keeps the skin younger and healthier and provides the means for better skin protection. Dormantin is used by many cosmetic companies as an active ingredient in cosmetic creams and lotions. Dormenin is sensitive to bacterial and fungal contamination.

[0154] Materials and methods

[0155] In this test, 100 microliters of Staphylococcus aureus culture was mixed with 5.8×10 7 Concentration of cfu / ml added to 2ml preservative-free Dormin TM solution (from IBR, Rehovot, Israel). Dormin inoculated with Staphylococcus aureus TM The solution was placed in a Petri dish coated with a 50 micron thick layer of Nafion™. After 4 hours and 22 hours of incubation at 30°C, samples were plated on TSA plates and incubated at 30°C for 24 hours to monitor bacterial proliferation.

[0156] result

[01...

Embodiment 3

[0161] Bacterial Inhibition in Commercially Available Cosmetic Creams Without Parabens

[0162] Materials and methods

[0163] Commercially available paraben-free cosmetic cream samples were from IBR Ltd., Rehovot, Israel. The initial E. coli and Staphylococcus aureus cultures were grown on TSB at 30° C. for 4 hours and mixed with the cosmetic cream in a 1:1 ratio (8 ml of each culture mixed with 8 g of the cosmetic cream) and placed in In a dish coated with Nafion™. Bacterial production was monitored at 0, 24, 48, 72, 96, 144 and 168 hours of incubation at 30°C as described above.

[0164] result

[0165] Figure 5 Shows bacterial production in cosmetic creams in Nafion™ coated dishes; Image 6 Shows bacterial production in cosmetic creams in Nafion™ coated dishes.

[0166] Figure 5 with Image 6 It was shown that bacterial growth of cosmetic creams was strongly inhibited in Nafion™ coated dishes compared to uncoated dishes. In fact, E. coli and Staphylococcus a...

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Abstract

The present invention presents a biocidic packaging for cosmetics and / or foodstuffs, comprises at least one insoluble proton sink or source (PSS). The ackaging is provided useful for killing living target cells (LTCs), or otherwise disrupting vital intracellular processes and / or intercellular interactions of said LTC upon contact. The PSS comprises, inter alia, (i) proton source or sink providing a buffering capacity; and (ii) means providing proton conductivity and / or electrical potential. The PSS is effectively disrupting the pH homeostasis and / or electrical balance within the confined volume of the LTC and / or disrupting vital intercellular interactions of the LTCs while efficiently preserving the pH of said LTCs' environment. The present invention also discloses a method for killing living target cells (LTCs), or otherwise disrupting vital intracellular processes and / or intercellular interactions of said LTC being in a packaging, especially cosmetic or foodstuffs' packaging.

Description

technical field [0001] The present invention relates to biocidal packaging for cosmetics and food products. The invention also relates to a method for avoiding contamination of cosmetics and food products in packaging. Background technique [0002] Generally, cosmetics and food are easily contaminated by bacteria, fungi, etc. To prevent such contamination, most cosmetic and food formulations contain preservatives commonly used in cosmetics and food that are required to prevent microbial contamination. Unfortunately, most preservatives added to cosmetics are toxic and can be skin irritating or cause infection. Very similarly, there is a continuing need for the food industry to eliminate or at least reduce the content of preservatives in food products. For clarity, the background section focuses first on the cosmetics industry and then on the food packaging industry. [0003] cosmetic [0004] Various preservative materials have been used in the cosmetic industry. One of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N61/00A01N25/10A01N25/34A01P1/00A01P15/00A61L2/16A61L27/34A61K45/00
CPCA01N25/34A01N37/20A23L3/3463A01N41/04A01N61/00A61L2/16A01N37/08A01N25/10
Inventor 什穆埃尔·比克什潘格莱布·齐尔伯施泰因
Owner OPLON
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