312 results about "Bacterial counts" patented technology

The present invention provides methods and compositions that enable tagging and amplification of targeted RNA molecules. A targeted RNA molecule is any non-polyadenylated RNA molecule including, for example, miRNA, siRNA, rRNA, tRNA, synthetic RNA, or non-polyadenylated mRNA such as mRNA from bacteria. In certain aspects, the invention provides methods and compositions for the genome-wide expression analysis of bacterial genes. Significantly, the methods enable genome-wide expression analysis in circumstances where bacterial numbers were previously too low to purify adequate amounts of RNA for DNA microarray analysis or other applications. Such methods are particularly useful for the study of bacterial gene expression during host-cell infection. The invention also provides kits for tagging and amplifying targeted RNA molecules.

Dairy or non-diary based fortified carbonated beverage solutions that supply essential nutrients in the human diet. The solution contains per 354 ml, calcium, magnesium and potassium ions in the form of salts and optionally vitamins A, D, C, lutein, zeaxanthin and folic acid in specified amounts to provide dietary supplementation. Sweeteners, stabilizers, flavors and carbonation can also be added to enhance flavor, taste, mouth-feel, ingredient solubilization and product appearance. A method of making the beverages is also described. A method of using carbonation to reduce bacterial counts and reduce degradation of essential nutrients in milk-based beverages with or without pasteurization is also disclosed.

Dairy or non-diary based fortified carbonated beverage solutions that supply essential nutrients in the human diet. The solution contains per 354 ml, calcium, magnesium and potassium ions in the form of salts and optionally vitamins A, D, C, lutein, zeaxanthin and folic acid in specified amounts to provide dietary supplementation. Sweeteners, stabilizers, flavors and carbonation can also be added to enhance flavor, taste, mouth-feel, ingredient solubilization and product appearance. A method of making the beverages is also described. A method of using carbonation to reduce bacterial counts and reduce degradation of essential nutrients in milk-based beverages with or without pasteurization is also disclosed.

Principal components of spice and herb plant solids are simultaneously extracted and concentrated with an edible solvent in a series of mixing and high temperature and pressure mechanical pressing steps using edible solvent and a countercurrent extraction procedure. The extract may be hydrated and then centrifuged to remove fine particulate solids and gums. A solution having several times the concentration of pigments and other flavor and aroma and / or antioxidant components of the starting raw material is obtained. The residual press solids may be cooled and hydrated following the last pressing operation. The residual press solids and extract have significantly reduced bacterial counts as a result of the temperatures, high pressure, and high shear utilized, as well as the low moisture levels employed, thus producing not only a food grade extract but also a food grade residual solid having low bacterial counts and predictable, standardized levels of the principal components of interest. The tissue of the residual spice or herb plant solid is ruptured in the process so as to provide quick release of the components thereof when utilized in a food or beverage, this greatly increasing its use effectiveness. The degree of browning or caramelization of the residual press solids is controlled, and the resistance to oxidative deterioration of both the extract and the residual solid is improved, by control of the temperature employed. Additionally, edible antioxidants can be included in the solvent to enhance the stability of both the extract and the residual solid.

The present invention relates to compounds of the formula I: or a pharmaceutically acceptable derivative or prodrug thereof. The compounds are useful as inhibitors of bacterial gyrase activity. The present invention also relates to methods for treating bacterial infections in mammala. The present invention also relates to methods for decreasing bacterial quantity in a biological sample.

The method of treatment for a periodontal tissue exhibiting a periodontal disease or periodontal condition in a diagnosed patient is disclosed. The method has the steps of activating an acoustic shock wave generator or source to emit acoustic shock waves; and subjecting the periodontal tissue, or the entire periodontal region of the patient to the acoustic shock waves stimulating said tissue, wherein the tissue is positioned within a path of the emitted shock waves. The method of treatment may further have the steps of administering one or more medicaments prior, during or after subjecting the patient to acoustic shock waves or testing the bacterial count or viability of the treated tissue or region of the diagnosed patient after exposure to one or more acoustic shock wave treatments; or subjecting a tissue or organ to a surgical procedure to remove or repair some or all of any defects or degenerative tissues. The method of treatment is for prevention of periodontal disease and may be used with debridement. The treatment is particularly useful in eradicating and inhibiting periodontal biofilms.

The invention provides a compound biological strain for feed. The compound biological strain comprises enterococcus faecalis, lactobacilli, saccharomycetes, bacillus subtilis and bacillus coagulans, wherein the bacterial count ratio of the enterococcus faecalis to the lactobacilli to the saccharomycetes to the bacillus subtilis to the bacillus coagulans is (0.5 to 1.5):(0.5 to 1.5):(0.1 to 0.5):(4 to 6):(0.5 to 1.5). The compound biological strain for feed and a preparation method of fermented feed provided by the invention have the advantages that the release of crude protein and amino acid in coarse feed can be promoted, and the animal absorption is facilitated.

A formulation comprising molecular arrangements capable of penetrating pores in a barrier, owing to penetrant adaptability, despite the fact that the average diameter of said pores is smaller than the average penetrant diameter, provided that the prenetrants can transport agents or else enable agent permeation through the pores after penetrants have entered pores, characterized in that the formulation comprises at least one consistency builder in an amount that increases the formulation to maximally 5 Nm / s so that spreading over, and retention at, the application area is enabled and / or at least one antioxidant in an amount that reduces the increase of oxidation index to less than 100% per 6 months and / or at least one microbiocide in an amount that reduces the bacterial count of 1 million germs added per g of total mass of the formulation to less than 100 in the case of aerobic bacteria, to less than 10 in the case of entero-bacteria, and to less than 1 in the case of Pseudomonas aeruginosa or Staphilococcus aureus, after a period of 4 days.

The method of treatment for a nasal or sinus tissue exhibiting a sinusitis or rhinosinusitis disease or condition in a diagnosed patient is disclosed. The method has the steps of activating an acoustic shock wave generator or source to emit acoustic shock waves; and subjecting the nasal or sinus tissue, or the entire nasal or sinus region of the patient to the acoustic shock waves stimulating said tissue, wherein the tissue is positioned within a path of the emitted shock waves. The method of treatment may further have the steps of administering one or more medicaments prior, during or after subjecting the patient to acoustic shock waves or testing the bacterial count or viability of the treated tissue or region of the diagnosed patient after exposure to one or more acoustic shock wave treatments; or subjecting a tissue or organ to a surgical procedure to remove or repair some or all of any defects or degenerative tissues. The method of treatment is for prevention of infectious disease and may be used with debridement. The treatment is particularly useful in eradicating and inhibiting biofilm formations.

The invention provides high-efficiency degradation nitrite pickled vegetable direct vat starter and a preparation method thereof. The high-efficiency degradation nitrite pickled vegetable direct vat starter comprises plantaricin, leuconostoc mesenteroides, pediococcus acidilactici and the freeze-dried fungus powder of lactobacillus brevis which are mixed by equal mass. The method prepares the high bacteria count starter under nutrition, growth and rich freeze-drying conditions by keeping bacterial strain with degradation nitrite through CICC, so the method has the advantages of product safety and no side effect. As determined, the bacterial count is equal to or more than 1010CFU / g. 150kg of Chinese cabbage is well fermented if fermented under 20 DEG C for 10 days; 150kg of Chinese cabbage is well fermented if fermented under 30 DEG C for 5 days; the mixed fermented degradation nitrite is equal to or more than 98 percent; the pickled vegetable has good flavor and excellent product quality. The invention can greatly shorten the vegetable fermentation time by 3 to 5 days, and can be used in industrialized production.

The invention relates to a rapid test method for microbial biomass using ATP bioluminescence and detection reagent cell, in which the said detection reagent cell consists of luciferase and its protecting agents, D-fluorescein, standard ATP, light-emitting detection buffer solution, non-cellular ATP remover, microbial cell ATP extractant and other agents. The rapid test standard method for microbial biomass using the detection reagent cell includes doing ATP normal curve in selected biological light-emitting device and selected system, obtaining linear regression equation after taking the logarithm, performing ATP biological light-emitting detection to specimen liquid by non-purpose ATP removal and micro-organism ATP extraction with the same enzyme in the same device and system, simultaneously doing blank control, then obtaining the specimen and blank CPM or RLU value, after taking the logarithm of the de-margined net relative luminescence value, and obtaining the mother ATP concentration with built regression equation. The ATP concentrations of bacteria, yeast, fungus and unicellular microalgae can be converted into the cell numbers of the said bacteria, yeast, fungus and unicellular microalgae, or homologous to the bacteria quantity.

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

The invention discloses a method for producing a rhodobacter sphaeroides microbial fertilizer from citric acid waste water, and belongs to the microorganism field. The method comprises the following steps: a. diluting citric acid waste water with clear water till the COD concentration is 12000-15000 mg / L, adjusting the pH to 6.5-7.5 with a sodium hydroxide aqueous solution, sterilizing the solution at 115-126 DEG C for 10-35 min; b. inoculating rhodobacter sphaeroides preserved by a slant into an erlenmeyer flask containing the citric acid waste water, performing shaking culture at 30-37 DEG C under 120-200 rpm for 24-48 hours to obtain liquid seeds; c. inoculating the liquid seeds into a fermentor containing the citric acid waste water with a volume ratio of 2%-20%, culturing the seeds in sterile air at 25-37 DEG C for 12-48 hours to obtain fermentation broth; d. determining the cultured fermentation broth by a spectrophotometer, packaging the broth when the OD660 value is 1.0-1.5 orthe bacterial count reaches 109-1010 CFU / ml so as to obtain a finished product. Compared with the prior art, the invention produces a microbial fertilizer from citric acid waste water, and has the characteristics of low production cost, energy saving and environmental protection, good product application effect, etc.

The invention discloses a biological special organic fertilizer applied to crops, in particular to a stubble regulating manure that eliminates the block of continuous cropping. The mixture ratio of rotten microbial inoculum is 30 percent of thermophil Bacillus subtilis, 20 percent of Bacillus megatherium, 10 percent of microzyme, 10 percent of Aspergillus flavus, 10 percent of seminude chactomium and 20 percent of Absidia corymbifera; the components of organic fertilizer are of 40 percent of chicken manure, 30 percent of dairy manure and 30 percent of straw powder; the process flow is that: the first fermentation is to graft rotten microbial inoculum in high quality organic fertilizer chicken manure and dairy manure to reach the standard of innocent treatment; the second fermentation is to take the organic fertilizer in the first fermentation as raw material and graft functional bacterial strain to lead the beneficial bacterial count in the organic fertilizer to reach the standard of biological fertilizer. As the invention chooses beneficial strain and adopts unique two-fermentation process, the produced stubble regulating manure can greatly adjust soil ecological balance, inhibit soil-borne disease, decrease the side effect of fertilizer and agricultural chemical, provide full nutrition for the growth of crops and improve the quality of agricultural products.

The invention discloses a fermentation device for rapidly composting vegetable wastes and a using method and effect of the fermentation device. The device is attractive in appearance and practical, is a hollow cylinder and comprises the following eight systems: a fermentation system, a sterilization system, a monitoring system, a ventilation system, a filtrate collection system, a heat insulation system, a deodorization system and a tool system; the device can be used for measuring temperature, humidity, gas and pressure sensing and can also be used for providing timely and comprehensive data for fermentation through combining with sampling analysis; steam sterilization and temperature and pressure control can be realized, the highest fermentation temperature is 45 DEG C, and the pressure is 0.02-0.03MPa; a sampling pipe, a discharging rake and a design that a material is prevented from falling from a fermentation chamber into an air distribution chamber are adopted; the rotten degree and bacterial count increasing, bacterium reducing and period shortening effects obtained by temperature and pressure controlled fermentation adopted by the device are better than those obtained by temperature and pressure uncontrolled fermentation and fermentation in a common plastic barrel with the same volume, the temperature and pressure controlled fermentation time is 10-12d, the temperature and pressure uncontrolled fermentation time is 19d, and the contrast time is 37d; after being treated by using the device, no fusarium wilts of cucumber are detected according to the contrast of 2*10<3> / g.

The invention provides a microbial organic fertilizer and a preparation method thereof. The preparation method comprises the steps of strain culture, compounding and liquid submerged fermentation, and belongs to an agricultural integrated production technique. 15-20% of Bacillus mucilaginosus, 15-18% of Bacillus megaterium, 15-18% of Azotobacter chroococcum, 10-13% of Saccharomyces cerevisiae, 10-13% of Candida utilis, 15-18% of Lactobacillus plantarum and 10-13% of actinomycete are respectively subjected to primary and secondary strain culture and tertiary liquid submerged fermentation until the bacterial count in the fermentation liquid reaches 10-15*10<8> / mL, thereby obtaining the microbial organic fertilizer (707(Y)). By adopting the advanced liquid submerged fermentation technique and using the strains with high stability and functionality, which are screened from the natural world over the years and have the advantages of many varieties and convenient application method, the invention can promote the plant growth, improve the fruit quality, promote prematurity, increase the yield, enhance the utilization ratio of the fertilizer, improve the ecological environment of soil, and have certain effects of disease prevention and disease resistance.

Methods and apparatus are provided for decreasing the bacteria count of a food commodity without affecting its overall organoleptic quality (taste, odor, and color). This is accomplished using a treatment fluid comprising ozone, which is injected into a treatment chamber containing the food commodity. Some water is preferably added to obtain better contact of the ozone with the food by forming a thin film of ozonated water on the food surface. Spices and / or other ingredients may preferably be added with the water. The food is placed in a tumbler and the tumbler is set in motion. During treatment good contact between the treatment fluid and the food commodity is obtained by reversibly oscillating the tumbler. A log reduction of 40% or more in bacteria count may be obtained as compared without the ozone.

The invention belongs to the field of zymocyte microbial inocula, and particularly relates to a pickle zymocyte microbial inoculum, and a preparation method and application thereof. The pickle zymocyte microbial inoculum is composed of the following components in parts by weight: 6-8 parts of skimmed milk powder, 5-8 parts of maltose, 5-8 parts of sodium glutamate, 90-110 parts of sterilized water and zymocyte; and on the basis of the weight parts of the sterilized water, the zymocyte bacterial count is 1-2 10<5>CFU / mL, and the zymocyte is composed of Bacillus coagulans and lactobacillus plantarum in a strain ratio of 1:(0.5-3). The nitrite content in the fermented pickle finished product is lower (1.5-3.0 ug / ml), the free amino acid content is higher (2.7-4.0 g / L), and the total amount of organic acids is higher (17.0-24.0 mg / mL); and the microbial inoculum has favorable sensory quality and greatly shortens the fermentation period to 4-8 days. After being subjected to 0.1Mpa vacuum packaging, thermal sterilization in an 85 DEG C water bath for 10 minutes, addition of 20mg / 100g potassium sorbate preservative and preservation for 3 months, the fermented pickle still contains the probiotic viable count of 1.0*10<3>-1.0*10<5>CFU / ml and has higher survival amount, and thus, can still maintain certain viable count after long shelf life of the product, so that the pickle has more outstanding health-care function.

The present invention provides a bacterium belonging to the genus Bifidobacterium, which can exhibit a high viability in the storage under agitation and can produce a fermented food containing a large number of viable bacterial count. The present invention further provides a fermented food, which contains the bacterium belonging to the genus Bifidobacterium and exhibits various physiological effects such as inhibitory action against harmful intestinal bacteria and intestinal function controlling effects. The present invention further provides a bacterium belonging to the genus Bifidobacterium having a rate of viability of 30% or more, when the bacterium is stored in the aerobic condition under agitation at 10° C. for 14 days after culturing in a medium containing milk as a main ingredient until obtaining a viable count of 1×108 cfu / ml or more, and fermented food using the same.

The invention provides a preparation method of strong aromatic Chinese spirits pit mud by adding red yeast esterified enzyme, and relates to the technical field of spirits making. The preparation method comprises the following steps of: adding 10kg of red yeast esterified enzyme and 10kg of yeast powder into mixed infiltrating body of 35kg of yellow clay, 3kg of old pit mud, 20kg of mud carbon, 5kg of bean cake powder, 5kg of double-wheel bottom flavour grains, 0.35kg of silkworm chrysalis powder, 2kg of yellow water, 10kg of hot vinasse, 10kg of 20 to 22-degree cocktail and 5kg of nutrient solution which respectively can pass a screen mesh of 10 and have the pH value of 5.5-6.0; vaccinating 10kg of compound hexanoic acid bacteria-enriched liquid, stirring and mixing to prepare pit mud grains; sealing the periphery of the pit mud grains by a plastic film; and cultivating for 30-35 days under the anaerobic condition at the temperature of 33-35 DEG C, and detecting the components and the beneficial microbe bacterial counts of the pit mud to obtain the strong aromatic Chinese spirits pit mud by adding the red yeast esterified enzyme. The preparation method is used for cultivating the pit mud in a strong aromatic Chinese spirits preparation technology. The preparation highlights a nitrogen source, trace element and the red yeast esterified enzyme which influences aroma-producing substances and flavor-producing substances of the Chinese spirits.

The invention discloses a fermentation device for the rapid thorough decomposition of livestock excrements and a using method and effects thereof. The device is beautiful and practical in design, has hollow cylinder shaped appearance, and comprises a fermentation system, a monitoring system, a ventilation system, a percolation and collection system and a heat preservation system. The device can carry out sensing determination on temperature and humidity, gas and pressure, and in combination with sampling analysis, provides timely and comprehensive data for the fermentation of livestock; the device can be subjected to full-sealed heat preservation, the temperature and pressure of the device can be controlled, the highest fermentation temperature is 55-60 DEG C, and the pressure is 0.02-0.03 Mpa; according to sampling, discharging, cleaning and appearance requirements, auxiliary tools such as a sampling tube and a discharge scraping rake are arranged and a design for preventing materials from falling into an air distribution chamber from a fermentation chamber is performed; under the action of temperature and pressure control and the like, the device is adopted for fermenting livestock excrements, through contrast in the aspects of available nutrients, bacterial count, escherichia coli and fermentation period, the fermentation effect of the device is better than those of temperature / pressure-uncontrollable devices and comparable common plastic buckets with a same volume, temperature / pressure-controllable fermentation is implemented in 12-15 days, and temperature / pressure-uncontrollable fermentation is implemented in 18 days, 42 days in contrast.

The invention discloses a preparation method of a straw decomposition agent. The straw decomposition agent is prepared by using bacillus subtilis, aspergillus oryzae, lactobacillus plantarum, trichoderma reesei and saccharomyces cerevisiae as preparation strains, carrying out activation and primary and secondary culture on the strains respectively to obtain various strain solutions with bacterial count of 106-108 / ml and mixing the strain solutions according to a certain proportion. The straw decomposition agent has the beneficial effects that the straw decomposition agent can be used for quickly degrading straws to prepare fertilizers and ensures that the straw decomposition time is at least 15 days ahead of the normal composting fermentation time; organic straw fertilizers prepared by the preparation method are used as base fertilizers, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

The invention discloses liquid compounding enzyme for brewage, and a preparation method of the liquid compounding enzyme, and belongs to the technical field of liquid compounding enzyme preparation. A liquid enzyme preparation for the brewage serves as a main raw material, stabilizing agents such as sugar, polyhydric alcohol, amino acid, a thickening agent, albumin and a metal ion and substances with preservative effects such as a hop extract promoting stabilization of the enzyme preparation, a Chinese herbal medicine extracting solution and antibacterial peptide are scientifically compounded, addition of a preservative is completely substituted, reproduction and growth of a harmful microbe are restrained effectively, and the activity of the liquid compounding enzyme can be cooperatively stabilized with other components, so that the biological stability and the enzyme activity stability of the liquid compounding enzyme are improved fundamentally, and the quality guarantee period of the liquid enzyme preparation is prolonged effectively. The aerobic bacterial count after the prepared liquid compounding enzyme is stored for 12 months at a room temperature is 168-214CFU / mL, and enzyme activity loss ratios after the liquid compounding enzyme is stored for 12 months at 40 DEGC and 0 DEG C are 2-2.5% and 1-1.7% respectively.

The invention provides a microbial feed additive and a preparation method thereof. The main processes are as follows: respectively culturing bacillus subtilis and bacillus licheniformis for 17-24 hours at 35-37 DEG C by using liquid media; mixing the bacillus subtilis and bacillus licheniformis with solid media which are sterilized and contain bran and grape seed extracts uniformly, and inoculating the bacillus subtilis and bacillus licheniformis into the solid media; then carrying out short solid fermentation (1-3 hours) at 35-37 DEG C; by utilizing the characteristic that bacillus is easy to generate spores when the environment changes, enabling the metabolically active bacterium to rapidly ripen and generate spores after short solid fermentation; and then grinding and packaging to prepare the microbial feed additive. The effective bacterial count of the microbial feed additive is not less than 1*108CFU / g. 0.1% of microbial feed additive is added to the feed for livestock and poultry to be used for reducing the rate of livestock and poultry diarrhea, reducing the infection rate, improving the feed intake and improving the daily gain.

The invention provides a beneficial fungus feed additive for large-scale composite edible fungi and a method for breeding flavored pigs. The beneficial fungus feed additive is formed by compounding a beneficial fungus mixture and the large-scale edible fungi, wherein the beneficial fungus mixture consists of lactobacillus, streptococcus faecalis, saccharomycetes, bacillus subtilis, bacillus subtilis natto and bacillus licheniformis, and live bacterial counts of the beneficial fungus mixture range from 0.8 to 1 billion / gram; the beneficial fungus mixture and the crushed fresh large-scale edible fungi are mixed in a mass ratio of 1:(0.8-1.2), and are placing at the temperature of between 33 and 37 DEG C for 3 to 5 days to obtain the beneficial fungus feed additive for the large-scale composite edible fungi. The method for breeding he flavored pigs comprises the following steps of: adding the beneficial fungus feed additive for the composite edible fungi into feed in a ratio of 0.1 to 0.5 mass percent feeding the pigs before slaughter for 30 to 60 days and thus, obtaining green flavored pork products.

The invention belongs to the technical field of agricultural microorganisms and particularly relates to a soil conditioner for resisting pseudo-ginseng continuous cropping diseases and improving the survival rate of pseudo-ginseng seedlings. The soil conditioner for resisting the pseudo-ginseng continuous cropping diseases comprises active components including bacillus subtilis, bacillus licheniformis and trichoderma fungi; and the bacterial count ratio bacillus subtilis to bacillus licheniformis to trichoderma fungi in the soil conditioner is (0.5-2) to (0.2-1.8) to (0.3-1.5). The soil conditioner is used for pseudo-ginseng continuous cropping fields and can be used for increasing the content of beneficial microorganisms in soil, reducing the production of harmful pathogenic bacteria, promoting decomposition of organic matter, effectively reducing yellow seedlings, weak seedlings and dead seedlings caused by continuous cropping, and improving the survival rate of the pseudo-ginseng seedlings.

A ready-to-use refrigerable liquid batter for preparing pancakes, waffles, brownies, muffins, cakes and the like comprises a starch component and an organic acid or a mixture of organic acids. The batter has been microbiologically stabilised by heating to a temperature below the gelatinisation temperature of the starch component for a time period sufficient to substantially reduce the bacterial count of the batter so as to make the batter storable for at least three weeks at a temperature of 8° C. or lower. Also disclosed is a process for preparing a batter of this kind.

The invention discloses an instrument and a method for bacterium flow count testing, relating to the biomedical detection technology. The instrument is provided with built-in glass micro channels and a flow system which enables a bacterium marked by a quantum dot probe to pass individually, and can realize high-sensitivity fast detection on the specificity of the bacterium by combining the quantum dot fluorescence excitation technology and the high-precision weak-light detection technology. The method comprises the following steps: after coupling a sample to be tested to a quantum dot probe for specificity, placing the sample into a detection injection port of the instrument to enable bacteria to flow with liquid through a glass micro-channel detection area, using a laser diode to excite and induce bacteria marked with quantum dots to fluoresce, using a photomultiplier tube to detect fluorescent signals, and calculating the tested bacterial count according to the detected light intensity and pulse count. The overall testing time of the instrument does not exceed 30 minutes. Compared with the conventional culture count method, the method for bacterium flow count testing has the advantages of strong specificity, high sensitivity, high detection speed and the like.

The invention provides a processing method of nutritional frozen Hibiscus esculentus slice. The method comprises the steps of material selection, color protection, sterilizing (ozone combined with microwave), freezing (cooling, and ultrasonic-assisted freezing), thawing, preparing, screening, slicing, quick-freezing, packaging, sealing and delivering. The method provided by the invention adopts ultrasonic-assisted freezing to solve tissue structure disintegration problem in single freezing process, effectively promotes nucleation under simultaneous mass-transfer action and heat-transfer action of ultrasound, avoids damage of functional ingredients in Hibiscus esculentus, and avoids nutritional ingredients loss by adopting two-step operation of freezing and sterilizing. Under joint action of ozone and microwave in sterilization process, the method has thorough sterilization and obvious effect; in combination with sterilization with 75% ethanol, bacterial count can be controlled at 500-300 / g, sterilization time is shortened by above 30%, and the product is safe, pollution-free and residue-free.