65 results about "Bacterial counts" patented technology

The invention belongs to the technical field of agricultural fertilizers, and discloses a preparation process and application of a medical stone double-effect organic fertilizer with soil improving and growth promoting functions, the medical stone double-effect organic fertilizer comprises the following components by weight: 20-40 parts of activated medical stone powder with a particle size of less than or equal to 0.1 mm, and activated by a citric acid solution; the decomposed organic material is prepared by mixing livestock and poultry manure and crop straw according to the mass ratio of 2: 1-4: 1 and performing aerobic composting fermentation through a compound microbial agent, and the water content is smaller than or equal to 30%; the microbial agent contains phosphate solubilizing bacteria, nitrogen-fixing bacteria and rhizosphere growth-promoting bacteria, and the effective viable count is greater than or equal to 2 * 10 CFU / g; the auxiliary material comprises bentonite, a polyacrylamide water-retaining agent and a pH (Potential of Hydrogen) regulator, and the proportion of the bentonite is greater than or equal to 30 percent. The adsorption-desorption performance of the medical stone is enhanced by activating the medical stone through citric acid, and a synergistic system of water retention, fertilizer supply and growth promotion is formed by combining decomposed organic materials and functional microorganisms. According to the fertilizer, the stability of a soil aggregate structure can be remarkably improved (increased by 15-20%), the yield increasing rate of crops reaches 20-30%, and 30% of chemical fertilizer dosage is reduced. The method is suitable for acid soil remediation, continuous cropping obstacle treatment and economic crop planting.

The application discloses a functional probiotic microencapsulated intestinal shield system and a preparation method and application thereof. A core layer comprises four probiotics, i.e., lactobacillus acidophilus La-14, animal bifidobacterium Bb-12, lactobacillus rhamnosus GG and lactobacillus reuteri DSM 17938, and the weight ratio is 3:4:2:1, and the total viable bacterial count is greater than or equal to 10^10 CFU / g. An inner layer is a targeted release control layer, which is composed of ethyl cellulose, polylactic acid, chitosan and lactose, can swell in a pH 5.5 to 6.5 environment and can dissolve in a pH 6.8 to 7.2 environment. A middle layer is a buffer protection layer, which is composed of calcium carbonate, disodium hydrogen phosphate and sodium alginate, and can maintain the internal pH of the microcapsule at 4.5 to 5.5 in a gastric acid environment. An outer layer is an intelligent response protection layer, which is composed of konjac glucomannan, xanthan gum and gum arabic, and can form a protective film in a gastric acid environment with a pH less than 3.0 within 5 minutes. Through the synergistic protection of the four-layer microcapsule structure, the probiotics can achieve a high survival rate in the gastric acid environment of cats, and can be accurately released at specific positions in the intestinal tract, which is helpful to improve the intestinal health of cats.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer combination and a kit for a peach brown rot pathogen dominant species Monilinia fraucticola and application of the LAMP detection primer combination and the kit. The primer combination comprises a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP and a reverse inner primer BIP, and nucleotide sequences of the forward outer primer F3, the reverse outer primer B3, the forward inner primer FIP and the reverse inner primer BIP are sequentially shown as SEQ ID NO.1-SEQ ID NO.4. The invention further discloses a kit for detecting the peach brown rot pathogen dominant species Monilinia fraucticola. According to the LAMP detection primer combination designed by aiming at the dominant species Monilinia fraticola, four specific primer combinations are designed by aiming at the specific gene BI-1 sequence of the Monilinia fraticola, the characteristic nucleic acid fragment of the Monilinia fraticola can be accurately identified in the detection environment such as the field, and the condition of cross reaction or misjudgment does not occur after repeated verification for multiple times. Meanwhile, due to the adaptability of the primer combination and the LAMP amplification technology, the detection limit concentration is as low as 1 * 10 <-2 > ng / mu L, stable detection can be realized even under the conditions of early infection of pathogenic bacteria and extremely low content of thalli in field samples, and a reliable technical basis is provided for early warning and source control of peach brown rot.

The present application belongs to the technical field of feed processing, and provides a puffed fermented phosphorus-reduced high-digestive-utilization rice by-product feed, which adopts a multi-layer structure design of CO2 guided puffed foaming extrusion to form a porous base material, sodium alginate and chitosan are alternately deposited to construct an interface modification layer, and compound enzymes and probiotics are solidly fermented, three-dimensional pore structure with a median pore size of 20-60 mu m and a connected pore ratio of greater than or equal to 60%, phytase activity of 5000-10000 FTU / kg, total viable bacterial count of greater than or equal to 1x10 8 CFU / g, and an acid value increment of less than or equal to 2 mgKOH / g after 90 days of storage, solves the technical problems of low utilization of rice by-product phytic acid phosphorus, low survival rate and easy inactivation of compound enzymes and probiotics, and poor storage stability of high-oil rice bran, and has wide application value in the feed industry.

The application discloses a plant Lactobacillus rhamnosus microcapsule and a preparation method and application thereof, and the plant Lactobacillus rhamnosus is embedded by adopting a low-temperature spray drying process with lily starch, citrus pectin and whey protein as wall materials, and mainly includes the following steps: preparation of a bacterial suspension, preparation of a wall material solution, preparation of a microcapsule solution and low-temperature spray drying for preparing the microcapsule. The preparation technology of the stable encapsulated plant Lactobacillus rhamnosus microcapsule provided by the application has the advantages that the viable bacterial count in the microcapsule is high after spray drying, the microcapsule has strong gastrointestinal fluid resistance, is stable in storage, and can effectively solve the problem that the bacterial activity of the plant Lactobacillus rhamnosus and products thereof is reduced or even inactivated in the production and processing, storage and transportation and in-vivo digestion process; the prepared microcapsule can be used as a probiotic bacterial agent and applied to foods such as cheese, yogurt, ice cream, milk, chocolate, lactic acid bacterial beverages and baked products, and helps to promote the healthy development of the probiotic industry, and has obvious economic and social benefits.

This invention discloses a lemongrass fermentation broth, its preparation method, and its application. The lemongrass fermentation broth simultaneously meets the following conditions: it contains live Lactiplantibacillus microorganisms, and the total number of live bacteria is ≥1×10⁻⁶. 9 The fermented lemongrass broth contains cfu / mL and contains volatile active ingredients, including at least linalool and methyl heptenone. Compared with unfermented lemongrass raw materials, the content of methyl heptenone in the lemongrass fermentation broth has increased from undetectable to over 10%, and the relative content of linalool has increased to over 20%. This invention's lemongrass fermentation broth possesses both a high viable bacterial count and a specific spectrum of volatile active ingredients. Through microbial fermentation, it achieves the targeted transformation of volatile components in lemongrass raw materials, particularly significantly increasing the content of linalool, methyl heptenone, and various ester aroma components, thus possessing broad prospects for industrial application.

Use of a composition comprising bacteria for odor reduction in the treatment of symptoms of tissue necrosis, wound secretions or putrid wounds or a disease selected from the group consisting of ozena, hyperhidrosis, osmidrosis, chronic urinary tract infections, chlamydial infections and halitosis; wherein the composition comprises the bacteria Lactobacillus acidophilus TYCA15, Lactobacillus salivarius, Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium longum in the ratio 1:1:1:1:1; and the composition totals 10 9 bacteria per gram of the composition.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

This invention belongs to the field of biopesticide technology, specifically relating to a biopesticide for controlling citrus thrips. The biopesticide, used to control citrus thrips, comprises an active ingredient derived from a compound of *Bretschneidera sinensis* powder and methyl salicylate, or a compound of *Bretschneidera sinensis* and ethyl spinosad; wherein the effective bacterial count of the *Bretschneidera sinensis* powder is 2.5 × 10⁻⁶. 8 Spores / g. This invention discovered that *Cladosporium flavum* is effective in killing citrus thrips and can be used as a microbial agent for the control of citrus thrips. Furthermore, this invention combines *Cladosporium flavum* with other existing bioactive ingredients and found that when combined with methyl salicylate or spinosad, it has a synergistic effect on killing citrus thrips. Compared with single-ingredient formulations, it can improve the control effect on citrus thrips, possessing the advantages of being green, safe, and highly efficient, and can provide support for the research and development of biological pesticides for the control of citrus thrips.

The invention belongs to the technical field of food microbial fermentation, and discloses a microbial agent for promoting ham fermentation and a preparation method thereof. The preparation method comprises the following steps: carrying out activation culture to obtain a bacterial suspension, centrifuging to remove culture medium residues, adding a composite protective agent prepared from lactose, skim milk powder, sucrose and maltose according to a certain ratio, and carrying out vacuum freeze-drying to prepare the microbial agent. The viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g, and the survival rate is still gt after the microbial inoculum is stored in a dark place at 4 DEG C for 1 month; 80%. When the bacterial agent is applied to ham fermentation, the color and luster and the elasticity of meat muscle fibers can be improved, meanwhile, the content of flavor substances is increased to 2.19 times that of a control group, the variety is also higher than that of the control group, and the ham fermentation process is remarkably promoted. The microbial agent has the advantages of low preparation cost, excellent storage stability, strong industrial adaptability and the like, and is suitable for large-scale ham production.

To provide a method for rapidly and accurately quantifying bacteria in a biological sample from a subject.SOLUTION: A method of determining an amount or concentration of bacteria in a sample, the method comprising: (a) amplifying a target nucleic acid of the bacteria from genetic material obtained from the sample to form an amplification product, wherein: (b) measuring the amount or concentration of the amplification product; (c) calculating the amount or concentration of the target nucleic acids in the sample by comparing the amount or concentration of the amplification product to a reference level thereof; and (d) determining the copy number of the bacterial 16SrRNA genes in the sample from the amount or concentration of the target nucleic acids in the sample, wherein: 16SrRNA, wherein said copy number is a function of or correlates with the amount of said bacteria in said sample.SELECTED DRAWING: None

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

The utility model provides a kind of bacterial counting device and water treatment system, it is related to the technical field of water quality microbiological detection device, sample pretreatment module includes filter assembly, and the water inlet end of filter assembly receives inlet water sample through pipeline, and the outlet end of filter assembly is connected with dyeing bin through pipeline, and pipeline intercommunication has drain pipe between filter assembly and dyeing bin, for the redundant water sample of outer discharge;CCD imaging module includes the imaging platform of pipeline intercommunication with the output end of dyeing bin, and automatic focusing assembly and fluorescence microscope are arranged on the upper side of the central axis of imaging platform, for showing the bacterial colony of fluorescent dye dyeing, and fluorescence microscope is electrically connected with CCD camera;CCD camera is electrically connected with image processing module, for outputting bacterial concentration and statistical distribution diagram, the technical problem of increasing culture time consumption and high detection cost of artificial operation in prior art, reaches fast detection, cost low and high accuracy technical effect.

The invention discloses a fermented chicory composition with xanthine oxidase inhibitory activity as well as a preparation method and application of the fermented chicory composition. The composition is prepared by taking chicory roots as a single plant raw material and fermenting the chicory roots with a composite flora consisting of lactobacillus plantarum, lactobacillus rhamnosus, bifidobacterium adolescentis and streptococcus thermophilus in a viable count ratio of 1: 1: 1: 1. The preparation method comprises the steps of culture medium preparation, inoculated fermentation and post-treatment. Through the synergistic fermentation effect of the specific flora and the chicory substrate, the xanthine oxidase inhibitory activity of the product is remarkably enhanced. Metabonomics analysis shows that various active ingredients such as phenolic acids and prebiotic oligosaccharides are remarkably enriched after fermentation, and a dual-action mechanism of directly inhibiting enzyme activity and indirectly regulating intestinal flora is formed. The composition is safe in raw materials and definite in effect, and can be used for preparing medicines, health-care foods or dietary supplements for prevention or adjuvant treatment of hyperuricemia, gout and related diseases.

The invention belongs to the technical field of biological mildew preventive, and discloses bacillus licheniformis fermentation liquor and application thereof in mildew prevention and quality improvement of silage. The fermentation liquor is prepared by taking bacillus licheniformis CMCC 63516 as a strain under optimized conditions (with an LB culture medium, the inoculum size of 6%, the liquid loading amount of 30% and culture for 18 hours), and the viable count can reach 1.48 * 10 < 9 > CFU / mL. The strain has a remarkable antagonistic effect on toxin-producing aspergillus flavus, can efficiently degrade aflatoxin B1 (the degradation rate gt in 36 hours is 96%), and has good environmental stability. The additive amount of 1.2-4.0% is applied to corn silage, the growth of mould and saccharomycetes can be remarkably inhibited, the content of AFB1 is greatly reduced, and the mould-proof effect is superior to that of a chemical mould-proof agent sodium diacetate. In addition, the fermentation liquor can also effectively improve the quality of silage, increase the content of crude protein and lactic acid, and reduce the pH and the content of ammoniacal nitrogen and detergent fibers. The invention provides a safe, environment-friendly and efficient biological mildew-proof method which has a wide application prospect in the feed industry.

The invention belongs to the technical field of quantitative detection of microorganisms, and particularly relates to a method for quantitatively detecting the viable count of mycoplasma bovis. The method for quantitatively detecting the viable count of the mycoplasma bovis is finally obtained by exploring the linear relation between the protein concentration and the viable count of multiple batches of mycoplasma bovis liquid samples after optimization of experimental conditions such as bacterium liquid preservation conditions, ultrasonic crushing modes, crushing duration and sample treatment modes, and the method is good in stability, high in timeliness and high in accuracy. The detection result is accurate, and a convenient and effective method is provided for rapid detection of the viable count of the mycoplasma bovis liquid.

The invention relates to the technical field of microbiological detection, and particularly discloses a bacterial colony number inversion method based on dynamic conductance response and organic carbon consumption characteristics, which comprises the following steps: acquiring dynamic conductance response data and organic carbon consumption data of a bacterial colony growth process in a sample to be detected, and synchronously acquiring environmental parameters of the sample to be detected; acquiring bacterial colony metabolism conductance parameters according to the dynamic conductance response data; acquiring a carbon base difference value and an organic carbon consumption rate according to the organic carbon concentration data, and acquiring an organic carbon characteristic parameter set according to the carbon base difference value and the organic carbon consumption rate; and establishing an optimization coefficient matrix according to the environmental parameters and the organic carbon characteristic parameter set. According to the method, the dynamic conductance response data and the organic carbon consumption characteristics are synchronously obtained, the optimization coefficient matrix is established in combination with the environmental parameters for real-time correction, environmental interference is effectively eliminated, and mixed strain recognition is achieved.

The invention relates to lactobacillus plantarum SJ-L-1 with high exopolysaccharide yield and application of the lactobacillus plantarum SJ-L-1 in preparation of red date juice rich in lactobacillus exopolysaccharide, and belongs to the technical field of microorganisms and food processing. The lactobacillus plantarum SJ-L-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.25209. The lactobacillus plantarum SJ-L-1 can be used for preparing the lactobacillus plantarum. The exopolysaccharide yield of the strain in an MRS culture medium is not lower than 0.4 mg / mL; after optimization of a specific fermentation process, the yield of exopolysaccharides in the red date juice can reach 11.283 mg / mL, which is increased by 220% compared with that of an unoptimized process, and is far higher than the existing reported level. Based on the characteristic of high-yield exopolysaccharide, the viable count of the obtained red date juice reaches 9.1 log CFU / mL, the hydroxyl radical scavenging rate is increased to 75%, and the flavor composition is also remarkably improved. The directional enrichment of functional components in the red date juice is realized through microbial fermentation, and a feasible technical path is provided for developing functional drinks with high exopolysaccharide content, excellent antioxidant activity and flavor quality.

The invention relates to the technical field of microbial preparations, and provides a microbial agent suitable for animal feed, which not only can improve the litter size and live litter size, but also can improve the birth weight of piglets and improve the survival rate of the piglets on the basis of solving constipation of sows by adopting probiotics. A microbial agent suitable for animal feed comprises 15 parts of bacterial powder, 45 parts of corn starch, 12 parts of skim milk powder, 8 parts of a yeast extract, 7 parts of a soybean extract and 6 parts of a rice bran extract. The bacterial powder is pediococcus pentosaceus bacterial powder, and the viable count of pediococcus pentosaceus in the microbial agent is not less than 1 * 10 < 6 > CFU / g.

To provide a method for enriching buckwheat seeds with GABA (γ-aminobutyric acid) by which a sufficient GABA-enriching effect is obtained in a relatively short time and the number of general viable bacteria which is an index of easiness of putrefaction and putrefaction is suppressed and to provide a method for producing buckwheat seeds highly containing GABA, buckwheat flour and a buckwheat processed product. And to provide buckwheat seeds highly containing GABA in which the GABA is contained in a fixed amount or more and the number of general viable bacteria is suppressed to a fixed amount or less.SOLUTION: The method for producing buckwheat seeds having a high GABA content comprises (S101) adding water to shell-removed buckwheat seeds, (S102) subjecting the seeds to a pressure treatment (S104) and then drying the seeds (S105). The buckwheat seeds having a high GABA content are buckwheat seeds subjected to a pressurizing treatment together with water, wherein the free GABA of the buckwheat seeds dried after the pressurizing treatment is equal to or more than 30mg / 100g dry matter, and the general viable cell count of the undried buckwheat seeds at 0 hour after the pressurizing treatment is 1. 0E + 04 or less.SELECTED DRAWING: Figure 1

The application belongs to the technical field of bacterial detection, and provides a nano composite probe system and application thereof, and a single-channel microfluidic chip for detecting bacterial quantity and bacterial drug resistance level, the nano composite probe system comprising phage-embedded magnetic bead probes and AIE@Ag@MnO2 NFs composite probes. The Ag-MnO2 nanoflower surface-modified AIE (Raman reporter molecule) composite probe can realize strong Raman signals, Ag@MnO2 catalyzes the decomposition of hydrogen peroxide to push the red dye to move and realize distance signals, and the magnetic bead-phage can realize magnetic separation and enrichment as a recognition element of target bacteria. Based on the high specificity and good environmental tolerance of the phage, the Ag-MnO2 nanoflower enhances the Raman signal of the AIE Raman reporter molecule, the double-mode signal internal correction realizes the stability of the sensor and the anti-interference of environmental and experimental factors, and improves the specificity of the detection strategy and the accuracy of on-site analysis.

The invention discloses a method for treating rice seeds, which is characterized in that a household direct drinking water purifier is combined with a hydrolysis ozone generator to prepare an aqueous solution containing 0.4 ppm ozone, and the purpose of sterilizing the rice seeds is achieved by soaking and cleaning the rice seeds. Experiments prove that the number of bacteria on the surfaces of the rice seeds treated by the direct drinking water containing the ozone concentration of 0.4 ppm is remarkably reduced, bacterial growth is inhibited, and germination and growth of the rice seeds are promoted. The rice seed surface disinfection method provided by the invention is simple and convenient to operate, has no residual toxicity, can effectively reduce and kill pathogenic microorganisms on the surfaces of rice seeds, and can promote germination and growth of rice seeds at the same time.

The present invention discloses a method for detecting the inhibition of antibiotics against bacteria, a bacteria counting device, and the method. The inhibition detection method includes the steps of: adding a predetermined concentration of antibiotic to a target bacterium to form a bacteria-drug mixture; and using a target bacterium without the antibiotic as a positive control; acquiring the current number of the bacteria in the bacteria-drug mixture and the current number of the bacteria in the positive control at a first predetermined time interval after the antibiotic is added; and determining whether the predetermined concentration of antibiotic inhibits, partially inhibits, or does not inhibit the bacteria based on the ratio of the current number of the bacteria in the bacteria-drug mixture to the current number of the bacteria in the positive control. The method is suitable for rapid drug susceptibility testing.

The invention relates to the technical field of fertilizers, and particularly discloses a cold-resistant compound microbial fertilizer and a preparation method thereof.The cold-resistant compound microbial fertilizer is prepared from cold-resistant microbial flora, an organic carrier, an inorganic anti-freezing synergistic component and a compound anti-freezing protective agent; according to the preparation method, the adaptability of a low-temperature environment can be remarkably improved, the retention rate of the total viable count is greater than 85% after storage at-15 DEG C for 3 months, the freezing point is reduced by 2.5-3.0 DEG C through microorganism domestication, gene modification and an anti-freezing system compounding, the growth of ice crystals is effectively inhibited, the survival rate of microorganisms at low temperature is guaranteed, and the survival rate of the microorganisms at low temperature is improved. A grid structure formed by the composite anti-freezing protective agent can buffer osmotic pressure change, and cell rupture is avoided.

The invention provides an ultraviolet sterilization and flocculation coupled activation water treatment device and a treatment method thereof, and relates to the technical field of water treatment.The ultraviolet sterilization and flocculation coupled activation water treatment device comprises a settling zone and a reaction zone of a flocculation settling pond, and the reaction zone is communicated with a water inlet main pipe; in the coupling activation assembly, one end of a raw water inlet pipeline is connected into raw water to be treated, the other end of the raw water inlet pipeline is sequentially communicated with the micro-flocculation device, the dual-band ultraviolet sterilization device and the pipeline mixer in the water inlet flowing direction, and the end, deviating from the dual-band ultraviolet sterilization device, of the pipeline mixer is communicated with a water inlet main pipe; the cationic polymer dosing assembly is communicated to an inlet pipeline of the micro-flocculation device, the flocculant dosing assembly is communicated to the pipeline mixer, the bacteria counting device is arranged at an inlet of the water inlet main pipe, and the camera assemblies are respectively arranged above the settling zone and the reaction zone; the control assembly is in signal connection with the coupling activation assembly, so that the problem of independent operation of the two assemblies in the prior art is solved, and the technical effects of enhancing sterilization in a water body and promoting the flocculation and agglomeration process of suspended matters are achieved.

The present application relates to the technical fields of microbial fermentation and feed processing, and discloses a multi-strain synergistic oxygen-consuming anaerobic feed fermentation method, which comprises the following steps: inoculating a compound fermentation inoculum containing an oxygen-consuming functional flora and an anaerobic functional flora into a feed fermentation substrate with adjusted moisture content and initial pH value at one time, and the ratio of viable bacterial count is 1:1.5-1:2.5; performing first-stage oxygen-consuming fermentation on the fermentation system, maintaining oxygen supply operation until the internal center temperature naturally rises to 40-45 DEG C; then stopping the oxygen supply operation and not performing secondary inoculation, directly applying mechanical pressure to the fermentation system to perform forced compaction and exhaust and performing physical sealing, so that the system performs second-stage anaerobic fermentation until the pH value drops to a preset acidic range, and fermented feed is prepared. The process conversion triggered by one-time inoculation and natural temperature rise avoids the introduction of external miscellaneous bacteria by segmented inoculation, and a low-oxygen high-acidity environment is constructed through microbial metabolites and physical sealing.

The objective is to provide a cleanliness evaluation device, a cleanliness evaluation method, and a program for evaluating the cleanliness of a storage facility that stores goods under high humidity and low temperature conditions. [Solution] A cleanliness evaluation device for a storage facility that stores articles under high humidity and low temperature conditions, comprising: an acquisition unit that acquires information regarding the number of bacteria and / or the types of bacteria in the storage facility at a first time point and a second time point after a predetermined time has elapsed from the first time point; and an evaluation unit that evaluates the cleanliness of the storage facility based on the information regarding the number of bacteria and / or the types of bacteria.

The present application relates to the field of microorganism, disclose a kind of composite microbial inoculant with desulfurization function and its application.The composite microbial inoculant includes: Thiobacillus thioparus and Gordonia alkanivorans;Wherein, the ratio of the viable bacterial count of Thiobacillus thioparus and Gordonia alkanivorans is 1:(0.05-5).The composite microbial inoculant provided by the present application can efficiently degrade sulfide, improve the desulfurization rate, and the desulfurization operation is simple, low in cost and free from secondary pollution.

The present application relates to the technical field of microbial inoculant, and particularly relates to a compound microbial inoculant and application of the compound microbial inoculant in preparation of chicken liver flavor seasoning, wherein the compound microbial inoculant is prepared by mixing Lactobacillus plantarum YR07 Lactiplantibacillus plantarum ) and Saccharomyces cerevisiae HQSAJ-001-24 Saccharomyces cerevisiae ) in a volume ratio of 1:1-3, wherein the Lactobacillus plantarum YR07 7 and the Saccharomyces cerevisiae HQSAJ-001-24 are respectively activated and cultured to a viable bacterial count of greater than or equal to 1x10 7 CFU / mL. Through the synergistic effect of the compound microbial inoculant fermentation and Maillard reaction, the inherent fishy smell of chicken liver is completely covered and transformed, bitter peptides are deeply degraded, umami and sweet amino acids are enriched, a compound system containing multiple key flavor substances is constructed, and finally a high-quality natural seasoning with rich flavor levels, fresh and mellow taste and significant flavor enhancement function is prepared.