120 results about "Bacterial counts" patented technology

The embodiment of the invention discloses a refrigerator bacterium detection method and system based on an optical fiber ATP sensor, and the method comprises the steps: mixing a sample in a to-be-detected area of a refrigerator with a reaction reagent to form a to-be-detected solution, injecting the to-be-detected solution into a microfluidic reaction tank of the optical fiber ATP sensor, enabling an ATP component and an enzyme reagent to generate a chemiluminescence reaction, and enabling an optical signal to enter a transmission optical fiber through focusing coupling; the optical signal is transmitted to a photoelectric detection unit through a transmission optical fiber to be converted into an electric signal, and the electric signal contains response dynamic information; carrying out multi-stage processing on the electric signal, and extracting a signal characteristic parameter representing the concentration of the ATP component; and finally, performing matching analysis on the signal characteristic parameters and preset data, determining an ATP component content value, querying corresponding relation data to generate a detection result containing bacteria quantity information, and outputting the detection result to a refrigerator matched display device in a visual form. The problems of long detection time, complicated operation, inconvenience in equipment, difficulty in in-situ real-time monitoring and the like in the existing refrigerator bacterium detection technology can be solved.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

The invention discloses preparation of a microbial compound element-based fertilizer and application of the microbial compound element-based fertilizer in saline-alkali soil treatment, and relates to the technical field of saline-alkali soil improvement. The preparation method comprises the following steps: inoculating lactic acid bacteria, rhodopseudomonas, phosphate-solubilizing bacillus, nitrogen-fixing bacillus, halophilic bacillus and halomonas into a composite culture medium in proportion, and carrying out stepped sequential fermentation culture under the conditions that the pH value is 7.5 and the temperature is 28 DEG C until the bacterial count is greater than or equal to 1010 CFU / mL. According to the preparation of the fertilizer based on the microbial compound elements and the application of saline-alkali soil treatment, a synergistic system of the compound functional flora, the chelated mineral substances and the intelligent organic carrier is constructed, so that multi-dimensional improvement of saline-alkali soil is realized, the limitation of a traditional single improvement mode is broken through, the salinity and pH value of the soil are effectively reduced, and the soil quality is improved. The soil fertility and biological activity are remarkably improved, the salt stress resistance of crops is enhanced, the effective period is prolonged, and the preparation cost is low.

The invention discloses a fabric antibacterial performance evaluation method and system, and relates to the technical field of antibacterial data processing. The method for evaluating the antibacterial performance of the fabric comprises the following steps: acquiring a bacterial data set after bacterial culture of a to-be-evaluated sample fabric; performing primary evaluation on the to-be-evaluated sample fabric based on the bacterial data set, and screening out primarily-screened sample fabric qualified in primary evaluation; acquiring physical characteristic data of the primarily screened sample fabric, and analyzing and screening a final qualified sample fabric based on the physical characteristic data; the fabric antibacterial evaluation qualification rate is determined based on the ratio of the sample fabric to the to-be-evaluated sample fabric, preliminary screening is performed, and the antibacterial effect of the fabric can be preliminarily determined; the physical characteristic data of the primarily screened sample fabric is obtained, the finally qualified sample fabric is screened out through physical characteristic data analysis, and the fabric antibacterial evaluation qualification rate is determined, so that the problems of incomplete data and insufficient accuracy in the fabric antibacterial effect evaluation process are effectively solved, and the scientificity and objectivity of antibacterial performance evaluation are enhanced.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention relates to the technical field of fermentation engineering, in particular to a method for increasing the yield of bacterial cellulose through blue-green algae. Comprising the following steps: carrying out fermentation by adopting Komagataeibacter xylinus to produce bacterial cellulose, and adding blue-green algae in the fermentation process; the ratio of the total viable count of the gluconacetobacter xylinum to the total viable count of the blue-green algae is (0.3-0.5): 1. According to the research, the problem that a compact film is formed on the surface of a culture medium by primarily generated bacterial cellulose can be remarkably solved by adding the blue-green algae (especially synechocystis) into a gluconacetobacter xylinum fermentation system, the cellulose production efficiency is improved, and the method has important value in the field of fermentation production of the bacterial cellulose.

The invention discloses a tea leaf theanine composition for protecting a central nervous system as well as a preparation method and application thereof, relates to the field of common foods and health-care foods, and aims to solve the problems that most of existing central nervous protection products are single components or are simply compounded, the synergistic effect is weak and the bioavailability is low. The preparation method comprises the following steps: S1, mixing the tea leaf theanine, the emblic leafflower fruit powder, the ginkgo leaf extract, the wolfberry fruit powder and other raw materials according to a specific ratio, and performing high-temperature sterilization and cooling to obtain a fermentation substrate; s2, inoculating a composite activation microbial inoculum composed of plant lactobacillus, lactobacillus acidophilus and lactobacillus reuteri, and carrying out constant-temperature anaerobic fermentation; and S3, freezing and centrifuging at 4 DEG C, concentrating with an RO membrane, and freezing and drying to obtain the composition. The viable count of the complex microbial inoculant reaches 2.0 * 10 < 9 >-3.0 * 10 < 9 > CFU / mL, and the strain ratio and the fermentation process are optimized. The composition has a synergistic effect of multiple components, can efficiently protect nervous centralis, and is suitable for preparing related common food, health food or functional food.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a mandarin fish prefabricated dish detection method and system and a computer readable storage medium. The mandarin fish prefabricated dish detection method comprises the following steps: acquiring a gel strength original value, an elasticity original value, a water activity original value, an RGB image, a temperature value and a bacteria amount original value of a sample; preprocessing the elasticity original value, the moisture activity original value, the RGB image and the bacteria amount original value to form a first feature vector and a second feature vector corresponding to the sample; calculating the first feature vector and the second feature vector by using a preset neural network model to obtain a first qualified weight corresponding to the sample; on the basis of the original gel strength value, the temperature value and a gel strength correction model, calculating a gel correction strength value corresponding to the sample, and on the basis of the gel correction strength value, obtaining a second qualified weight corresponding to the sample; and on the basis of the first qualified weight and the second qualified weight, calculating a qualified coefficient of the sample, and outputting a second signal value that the sample is unqualified.

The invention belongs to the technical field of agricultural fertilizers, and discloses a preparation process and application of a medical stone double-effect organic fertilizer with soil improving and growth promoting functions, the medical stone double-effect organic fertilizer comprises the following components by weight: 20-40 parts of activated medical stone powder with a particle size of less than or equal to 0.1 mm, and activated by a citric acid solution; the decomposed organic material is prepared by mixing livestock and poultry manure and crop straw according to the mass ratio of 2: 1-4: 1 and performing aerobic composting fermentation through a compound microbial agent, and the water content is smaller than or equal to 30%; the microbial agent contains phosphate solubilizing bacteria, nitrogen-fixing bacteria and rhizosphere growth-promoting bacteria, and the effective viable count is greater than or equal to 2 * 10 CFU / g; the auxiliary material comprises bentonite, a polyacrylamide water-retaining agent and a pH (Potential of Hydrogen) regulator, and the proportion of the bentonite is greater than or equal to 30 percent. The adsorption-desorption performance of the medical stone is enhanced by activating the medical stone through citric acid, and a synergistic system of water retention, fertilizer supply and growth promotion is formed by combining decomposed organic materials and functional microorganisms. According to the fertilizer, the stability of a soil aggregate structure can be remarkably improved (increased by 15-20%), the yield increasing rate of crops reaches 20-30%, and 30% of chemical fertilizer dosage is reduced. The method is suitable for acid soil remediation, continuous cropping obstacle treatment and economic crop planting.

This invention relates to the field of microbial technology, and particularly to a compound lactic acid bacteria fermentation agent and its application, a method for producing *Anoectochilus roxburghii* fermentation broth, and related products. The compound lactic acid bacteria fermentation agent comprises *Lactobacillus plantarum* BXM2, *Lactobacillus fermentatus* J IAN, and *Lactobacillus paracasei* YYS-69. Fermenting *Anoectochilus roxburghii* broth using this compound fermentation agent results in stronger acid production, higher viable bacterial counts, and significantly improved antioxidant activity, total polyphenol and flavonoid content, and enhanced hypoglycemic activity. Sensory effects are also significantly improved. This compound lactic acid bacteria fermentation agent, when applied to the fermentation of traditional Chinese medicine, can enhance the antioxidant capacity and effective functional component content of the fermentation broth, thereby improving efficacy. It has great application prospects in the field of deep-processed *Anoectochilus roxburghii* products and even other fermented medicinal and edible products.

The application discloses a functional probiotic microencapsulated intestinal shield system and a preparation method and application thereof. A core layer comprises four probiotics, i.e., lactobacillus acidophilus La-14, animal bifidobacterium Bb-12, lactobacillus rhamnosus GG and lactobacillus reuteri DSM 17938, and the weight ratio is 3:4:2:1, and the total viable bacterial count is greater than or equal to 10^10 CFU / g. An inner layer is a targeted release control layer, which is composed of ethyl cellulose, polylactic acid, chitosan and lactose, can swell in a pH 5.5 to 6.5 environment and can dissolve in a pH 6.8 to 7.2 environment. A middle layer is a buffer protection layer, which is composed of calcium carbonate, disodium hydrogen phosphate and sodium alginate, and can maintain the internal pH of the microcapsule at 4.5 to 5.5 in a gastric acid environment. An outer layer is an intelligent response protection layer, which is composed of konjac glucomannan, xanthan gum and gum arabic, and can form a protective film in a gastric acid environment with a pH less than 3.0 within 5 minutes. Through the synergistic protection of the four-layer microcapsule structure, the probiotics can achieve a high survival rate in the gastric acid environment of cats, and can be accurately released at specific positions in the intestinal tract, which is helpful to improve the intestinal health of cats.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection primer combination and a kit for a peach brown rot pathogen dominant species Monilinia fraucticola and application of the LAMP detection primer combination and the kit. The primer combination comprises a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP and a reverse inner primer BIP, and nucleotide sequences of the forward outer primer F3, the reverse outer primer B3, the forward inner primer FIP and the reverse inner primer BIP are sequentially shown as SEQ ID NO.1-SEQ ID NO.4. The invention further discloses a kit for detecting the peach brown rot pathogen dominant species Monilinia fraucticola. According to the LAMP detection primer combination designed by aiming at the dominant species Monilinia fraticola, four specific primer combinations are designed by aiming at the specific gene BI-1 sequence of the Monilinia fraticola, the characteristic nucleic acid fragment of the Monilinia fraticola can be accurately identified in the detection environment such as the field, and the condition of cross reaction or misjudgment does not occur after repeated verification for multiple times. Meanwhile, due to the adaptability of the primer combination and the LAMP amplification technology, the detection limit concentration is as low as 1 * 10 <-2 > ng / mu L, stable detection can be realized even under the conditions of early infection of pathogenic bacteria and extremely low content of thalli in field samples, and a reliable technical basis is provided for early warning and source control of peach brown rot.

The invention discloses a preparation method of an acinetobacter picoteri solid fungicide, and relates to the field of a production process of a microbial solid fungicide, in particular to the preparation method of the acinetobacter picoteri solid fungicide. The invention aims to solve the problem of low viable count in the existing acinetobacter picoteri microbial inoculum. The method comprises the following steps of: 1, transferring a cryopreserved acinetobacter peteri strain into a slant activation culture medium, and culturing at a constant temperature; 2, after activation is completed, two rings of strains are scraped from the slant activation culture medium through an inoculating loop and inoculated to a seed culture medium, and a seed solution is obtained through constant-temperature shaking culture; 3, inoculating the seed solution into a conical flask filled with a fermentation culture medium for culturing; and 4, adding the acinetobacter picoteri liquid subjected to shake flask fermentation into spray drying equipment for spray drying, and adding a spray drying composite protective agent to prepare the acinetobacter picoteri powder. According to the method disclosed by the invention, the viable count and the thallus survival rate of the acinetobacter pitertii spray drying are remarkably improved. The method is used for preparing the acinetobacter picoteri solid microbial inoculum.

The present application belongs to the technical field of feed processing, and provides a puffed fermented phosphorus-reduced high-digestive-utilization rice by-product feed, which adopts a multi-layer structure design of CO2 guided puffed foaming extrusion to form a porous base material, sodium alginate and chitosan are alternately deposited to construct an interface modification layer, and compound enzymes and probiotics are solidly fermented, three-dimensional pore structure with a median pore size of 20-60 mu m and a connected pore ratio of greater than or equal to 60%, phytase activity of 5000-10000 FTU / kg, total viable bacterial count of greater than or equal to 1x10 8 CFU / g, and an acid value increment of less than or equal to 2 mgKOH / g after 90 days of storage, solves the technical problems of low utilization of rice by-product phytic acid phosphorus, low survival rate and easy inactivation of compound enzymes and probiotics, and poor storage stability of high-oil rice bran, and has wide application value in the feed industry.

A bacterial detection module, which solves the problems of a long detection cycle, unsuitability for civilian use and the like of a bacterial quantity measurement method. The bacterial detection module comprises a housing (1), and an excitation light source (2) and a fluorescence detection component (3) which are provided in a first cavity (11) in the housing (1). The excitation light source (2) is configured to be capable of emitting excitation light after being powered on, and the excitation light can excite microorganisms carried in air under test to emit fluorescence; and the fluorescence detection component (3) is configured to be capable of detecting the fluorescence emitted by the microorganisms carried in the air under test after the air under test is irradiated by the excitation light after the fluorescence detection component is powered on, and outputting a detection result. By providing the excitation light source (2) and the fluorescence detection component (3), and providing the excitation light source (2) and the fluorescence detection component (3) in the same cavity (11), the number of microorganisms carried in the air under test can be timely and accurately measured without sampling and culturing and providing a complex optical pathway. The present invention involves a simple and rapid detection process, and can be widely applied to various scenarios.

The application discloses a plant Lactobacillus rhamnosus microcapsule and a preparation method and application thereof, and the plant Lactobacillus rhamnosus is embedded by adopting a low-temperature spray drying process with lily starch, citrus pectin and whey protein as wall materials, and mainly includes the following steps: preparation of a bacterial suspension, preparation of a wall material solution, preparation of a microcapsule solution and low-temperature spray drying for preparing the microcapsule. The preparation technology of the stable encapsulated plant Lactobacillus rhamnosus microcapsule provided by the application has the advantages that the viable bacterial count in the microcapsule is high after spray drying, the microcapsule has strong gastrointestinal fluid resistance, is stable in storage, and can effectively solve the problem that the bacterial activity of the plant Lactobacillus rhamnosus and products thereof is reduced or even inactivated in the production and processing, storage and transportation and in-vivo digestion process; the prepared microcapsule can be used as a probiotic bacterial agent and applied to foods such as cheese, yogurt, ice cream, milk, chocolate, lactic acid bacterial beverages and baked products, and helps to promote the healthy development of the probiotic industry, and has obvious economic and social benefits.

The invention belongs to the technical field of bacterial detection, and provides a nano-composite probe system, application thereof and a single-channel micro-fluidic chip for detecting the number of bacteria and the drug resistance level of the bacteria, and the nano-composite probe system comprises a phage embedded magnetic bead probe and an AIE (at) Ag (at) MnO2NFs composite probe. The Ag-MnO2 nanoflower surface modified AIE (Raman reporter molecule) composite probe can realize a strong Raman signal, Ag-coated MnO2 catalyzes decomposition of hydrogen peroxide to push a red dye to move so as to realize a distance signal, and magnetic bead-bacteriophage is used as a recognition element of target bacteria and realizes magnetic separation and enrichment at the same time. Based on high specificity and good environmental tolerance of the phage, the Ag-MnO2 nanoflowers enhance Raman signals of AIE Raman reporter molecules, dual-mode signal internal correction realizes the stability of the sensor and the interference resistance to factors such as environment and experiments, and the specificity of a detection strategy and the accuracy of on-site analysis are improved.

This invention discloses a lemongrass fermentation broth, its preparation method, and its application. The lemongrass fermentation broth simultaneously meets the following conditions: it contains live Lactiplantibacillus microorganisms, and the total number of live bacteria is ≥1×10⁻⁶. 9 The fermented lemongrass broth contains cfu / mL and contains volatile active ingredients, including at least linalool and methyl heptenone. Compared with unfermented lemongrass raw materials, the content of methyl heptenone in the lemongrass fermentation broth has increased from undetectable to over 10%, and the relative content of linalool has increased to over 20%. This invention's lemongrass fermentation broth possesses both a high viable bacterial count and a specific spectrum of volatile active ingredients. Through microbial fermentation, it achieves the targeted transformation of volatile components in lemongrass raw materials, particularly significantly increasing the content of linalool, methyl heptenone, and various ester aroma components, thus possessing broad prospects for industrial application.

Use of a composition comprising bacteria for odor reduction in the treatment of symptoms of tissue necrosis, wound secretions or putrid wounds or a disease selected from the group consisting of ozena, hyperhidrosis, osmidrosis, chronic urinary tract infections, chlamydial infections and halitosis; wherein the composition comprises the bacteria Lactobacillus acidophilus TYCA15, Lactobacillus salivarius, Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium longum in the ratio 1:1:1:1:1; and the composition totals 10 9 bacteria per gram of the composition.

The application discloses application of Clostridium herbivorans in improving litter performance of mammals, and a probiotic preparation containing Clostridium herbivorans, wherein the viable bacterial count of Clostridium herbivorans in the probiotic preparation is 10 6 ~10 10 million / mL. After the Clostridium herbivorans colonizes in the intestinal tract of mice, the number of litters and the number of live litters of the mice can be improved.

This invention belongs to the field of biopesticide technology, specifically relating to a biopesticide for controlling citrus thrips. The biopesticide, used to control citrus thrips, comprises an active ingredient derived from a compound of *Bretschneidera sinensis* powder and methyl salicylate, or a compound of *Bretschneidera sinensis* and ethyl spinosad; wherein the effective bacterial count of the *Bretschneidera sinensis* powder is 2.5 × 10⁻⁶. 8 Spores / g. This invention discovered that *Cladosporium flavum* is effective in killing citrus thrips and can be used as a microbial agent for the control of citrus thrips. Furthermore, this invention combines *Cladosporium flavum* with other existing bioactive ingredients and found that when combined with methyl salicylate or spinosad, it has a synergistic effect on killing citrus thrips. Compared with single-ingredient formulations, it can improve the control effect on citrus thrips, possessing the advantages of being green, safe, and highly efficient, and can provide support for the research and development of biological pesticides for the control of citrus thrips.

The invention relates to the technical field of microbial strains, and provides torulaspora delbrueckii, and the preservation number of the torulaspora delbrueckii is CGMCC (China General Microbiological Culture Collection Center) NO.7.581. The invention further discloses a screening and identifying culture medium which is used for screening, identifying and culturing the torulaspora delbrueckii. The screening and identifying culture medium comprises a nutrient agar culture medium, a sugar fermentation culture medium, an assimilation carbon source culture medium, an assimilation nitrogen source culture medium and a YPD basal culture medium. The torulaspora delbrueckii is screened from the mousse fruit wine and is used for mousse fermentation, the defect that the taste and flavor of existing saccharomycetes are similar in the fermentation process can be overcome, and the traditional flavor and taste of mousse can be reserved. And the contents of glucose, peptone and yeast extract powder in the culture medium are optimized through a single factor test and a response surface method, so that the amount of torulaspora delbrueckii is further increased, and theoretical guidance is provided for industrial application of mousalaisi brewing.

The invention belongs to the technical field of food microbial fermentation, and discloses a microbial agent for promoting ham fermentation and a preparation method thereof. The preparation method comprises the following steps: carrying out activation culture to obtain a bacterial suspension, centrifuging to remove culture medium residues, adding a composite protective agent prepared from lactose, skim milk powder, sucrose and maltose according to a certain ratio, and carrying out vacuum freeze-drying to prepare the microbial agent. The viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g, and the survival rate is still gt after the microbial inoculum is stored in a dark place at 4 DEG C for 1 month; 80%. When the bacterial agent is applied to ham fermentation, the color and luster and the elasticity of meat muscle fibers can be improved, meanwhile, the content of flavor substances is increased to 2.19 times that of a control group, the variety is also higher than that of the control group, and the ham fermentation process is remarkably promoted. The microbial agent has the advantages of low preparation cost, excellent storage stability, strong industrial adaptability and the like, and is suitable for large-scale ham production.

To provide a method for rapidly and accurately quantifying bacteria in a biological sample from a subject.SOLUTION: A method of determining an amount or concentration of bacteria in a sample, the method comprising: (a) amplifying a target nucleic acid of the bacteria from genetic material obtained from the sample to form an amplification product, wherein: (b) measuring the amount or concentration of the amplification product; (c) calculating the amount or concentration of the target nucleic acids in the sample by comparing the amount or concentration of the amplification product to a reference level thereof; and (d) determining the copy number of the bacterial 16SrRNA genes in the sample from the amount or concentration of the target nucleic acids in the sample, wherein: 16SrRNA, wherein said copy number is a function of or correlates with the amount of said bacteria in said sample.SELECTED DRAWING: None

The invention relates to a complex microbial inoculant for synchronously degrading microplastics and denitrifying, a layered embedding method of the complex microbial inoculant and application of the complex microbial inoculant in a sediment environment, and belongs to the technical field of microorganisms. And the viable count ratio of the microbacterium ester, the bacillus subtilis and the pseudomonas vanillis in the complex microbial inoculant is 4: 5: 2, and the microbacterium ester, the bacillus subtilis and the pseudomonas vanillis in the complex microbial inoculant are compounded. The layered embedding method is as follows: the inner layer is microbacterium ester and an iron element; the middle layer is pseudomonas (denitrifying bacteria) which forms an anoxic denitrification zone; the outer layer is formed by cellulose and bacillus subtilis to form a carbon source slow-release layer. According to the application, the complex microbial inoculant is added into sediments of rivers and lakes. The method not only can efficiently degrade polyethylene and polypropylene microplastics in a targeted manner, but also can synchronously realize nitrogen removal by denitrification, has the advantages of high remediation efficiency, strong environmental adaptability, good ecological safety and the like, and is suitable for combined pollution treatment of anoxic and light-proof sediment environments such as urban rivers and lakes.

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

The utility model provides a kind of bacterial counting device and water treatment system, it is related to the technical field of water quality microbiological detection device, sample pretreatment module includes filter assembly, and the water inlet end of filter assembly receives inlet water sample through pipeline, and the outlet end of filter assembly is connected with dyeing bin through pipeline, and pipeline intercommunication has drain pipe between filter assembly and dyeing bin, for the redundant water sample of outer discharge;CCD imaging module includes the imaging platform of pipeline intercommunication with the output end of dyeing bin, and automatic focusing assembly and fluorescence microscope are arranged on the upper side of the central axis of imaging platform, for showing the bacterial colony of fluorescent dye dyeing, and fluorescence microscope is electrically connected with CCD camera;CCD camera is electrically connected with image processing module, for outputting bacterial concentration and statistical distribution diagram, the technical problem of increasing culture time consumption and high detection cost of artificial operation in prior art, reaches fast detection, cost low and high accuracy technical effect.