190 results about "Bacterial counts" patented technology

The invention belongs to the technical field of immobilized microorganisms and microbial environment restoration, and particularly relates to a chitosan modified charcoal immobilized complex microbial inoculant as well as a preparation method and application thereof. The chitosan modified biochar immobilized complex microbial inoculant provided by the invention comprises complex bacteria, biochar and chitosan, the complex bacteria are fixedly attached to the surface of the biochar and the interiors of pores, and the chitosan forms a layer of uniform membrane structure on the surface of the biochar. The chitosan modified biochar immobilized complex microbial inoculant not only improves the viable count and stability of immobilized microorganisms, but also can significantly improve the degradation rate of pollutants, has good acid-base tolerance and salt tolerance, can be suitable for pollutant degradation and environment restoration or improvement in various acid-base environments and salt environments, and has wide application prospects. The method has good environmental adaptability and good application prospects.

The embodiment of the invention discloses a refrigerator bacterium detection method and system based on an optical fiber ATP sensor, and the method comprises the steps: mixing a sample in a to-be-detected area of a refrigerator with a reaction reagent to form a to-be-detected solution, injecting the to-be-detected solution into a microfluidic reaction tank of the optical fiber ATP sensor, enabling an ATP component and an enzyme reagent to generate a chemiluminescence reaction, and enabling an optical signal to enter a transmission optical fiber through focusing coupling; the optical signal is transmitted to a photoelectric detection unit through a transmission optical fiber to be converted into an electric signal, and the electric signal contains response dynamic information; carrying out multi-stage processing on the electric signal, and extracting a signal characteristic parameter representing the concentration of the ATP component; and finally, performing matching analysis on the signal characteristic parameters and preset data, determining an ATP component content value, querying corresponding relation data to generate a detection result containing bacteria quantity information, and outputting the detection result to a refrigerator matched display device in a visual form. The problems of long detection time, complicated operation, inconvenience in equipment, difficulty in in-situ real-time monitoring and the like in the existing refrigerator bacterium detection technology can be solved.

The invention discloses a method for detecting the concentration of bacteria in water based on an image processing technology, and relates to the technical field of computer image processing. The method for detecting the concentration of bacteria in water based on the image processing technology comprises the following steps: acquiring preliminary detection accuracy; acquiring an area scanning evaluation value; generating a superpixel; and acquiring the concentration of the to-be-detected bacteria. According to the method, the to-be-detected sample data is preliminarily marked through the fluorescent marker to obtain the preliminary detection accuracy, meanwhile, the to-be-detected region division process in the laser layer-by-layer scanning operation process is monitored in real time to obtain the region scanning evaluation value, and then the obtained three-dimensional image is subjected to superpixel segmentation to generate superpixels; finally, the number of the to-be-detected bacteria in the superpixels is counted, and the concentration of the to-be-detected bacteria is obtained in combination with the preset experimental group water sample volume, so that the effect of improving the reliability of detecting the concentration of the bacteria in water based on data processing is achieved, and the problem that the reliability of detecting the concentration of the bacteria in water based on data processing is low in the prior art is solved.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

The invention discloses high-activity plant lactobacillus freeze-dried powder and a preparation method and application thereof.According to the method, viable bacteria of plant lactobacillus fermentation liquor are increased to 1.66 * 10 < 10 >-1.82 * 10 < 10 > CFU / mL through a high-density fermentation and one-time material supplementing technology, the technology is low in production cost and easy to operate, and the problem that the viable bacteria number of the plant lactobacillus fermentation liquor is low is effectively solved. The freeze-drying protective agent is optimized by adding the plant peptide, the animal peptide and the polyphenol into the basic protective agent, the viable count of the plant lactobacillus freeze-dried powder can be increased to 1.25 * 10 < 12 >-2.55 * 10 < 12 > CFU / g, and the key problem of preparation of high-activity probiotic powder is effectively solved. At the same time, the addition of the active substances enriches the nutrition and efficacy of the freeze-dried powder, and provides a certain experimental basis for the development and application of plant lactobacillus functional food.

The invention relates to the technical field of kitchen waste treatment, in particular to a solid fungicide, a preparation method and application. The solid microbial inoculum comprises a microbial inoculum and a carrier; the microbial agent is prepared from pseudoochrobactrum saccharum, alcaligenes faecalis, achromobacter xylosoxidans, paenibacillus amyloliquefaciens, bacillus cereus, sphingobium nicotianae, serratia marcescens, stenotrophomonas maltophilia, sphingobium humus, sphingobium aquaticum and bacillus subtilis; the carrier for carrying the liquid compound bacteria is bagasse, and the maximum number of released bacteria is 2.2 * 10 < 9 > CFU / g. When the solid microbial inoculum is added into a 18 L kitchen waste reactor, the waste decrement rate is 80% within 7 days, and the COD removal rate can reach 79.1% at most. According to the solid microbial agent disclosed by the invention, organic substances in the kitchen waste can be degraded while the kitchen waste is converted from a solid state to a liquid state, so that volume reduction and weight reduction of the kitchen waste are realized; and the solid microbial inoculum is convenient to transport and operate and low in cost, has positive significance on difficulties faced by kitchen waste treatment, and has a good application prospect.

The invention relates to the technical field of normal-temperature yoghurt, in particular to normal-temperature yoghurt and a preparation method thereof. By combining double-frequency ultrasonic treatment and ultrahigh pressure treatment and applying ultrasonic treatment in the second-stage fermentation process between the first-stage fermentation and the third-stage fermentation, the drawing characteristic of the yoghourt can be greatly improved on the basis of maintaining relatively low bacterial count and even sterility of the yoghourt; the yogurt can be stored at normal temperature and has a good wire drawing effect. Excessive high-pressure treatment pressure or too long treatment time can cause damage to the structure of the yogurt and incapability of drawing the yogurt, and too low high-pressure treatment pressure and too short treatment time can cause excessive lactic acid content of the yogurt, so that the yogurt cannot be stored at normal temperature and only can be stored at low temperature. According to the invention, the pressure and time of the ultrahigh pressure treatment are controlled within the range, so that a good wire drawing effect is obtained, and the low bacterial count and even sterility of the yoghourt are maintained.

The invention provides a method for determining the number of bacteria in cathode electrophoretic paint. The method comprises the steps that a relation model of the hydrogen ion concentration and the number of bacteria in the cathode electrophoretic paint is determined; the real-time pH value of the cathode electrophoretic paint is obtained; based on the real-time pH value, the real-time hydrogen ion concentration in the cathode electrophoretic paint is obtained; and determining the real-time bacterial quantity in the cathode electrophoretic paint based on the relation model and the real-time hydrogen ion concentration. According to the method for determining the number of the bacteria in the cathode electrophoretic paint, the real-time hydrogen ion concentration in the cathode electrophoretic paint is obtained by collecting the pH value data of the cathode electrophoretic paint in real time, and then the real-time number of the bacteria in the cathode electrophoretic paint is obtained according to the relation model of the hydrogen ion concentration and the number of the bacteria in the cathode electrophoretic paint. According to the method, the number of bacteria in the cathode electrophoretic paint can be obtained in time, the operation is simple, and the cost is relatively low.

The invention discloses application of fludarabine in treatment of methicillin-resistant staphylococcus aureus infection, and in an MRSA infected mouse model, it is verified that fludarabine can improve the survival rate of MRSA infected mice, reduce the MRSA bacterial count of lungs, livers, spleens and kidneys and relieve the injury degree of lungs, livers, spleens and kidneys caused by MRSA infection; pSMalpha3 is found to be an important virulence factor of MRSA, is closely related to high pathogenicity of MRSA, and can induce death of macrophages in a concentration-dependent manner, and fludarabine can significantly reduce cytotoxicity caused by PSMalpha3.

The invention discloses preparation of a microbial compound element-based fertilizer and application of the microbial compound element-based fertilizer in saline-alkali soil treatment, and relates to the technical field of saline-alkali soil improvement. The preparation method comprises the following steps: inoculating lactic acid bacteria, rhodopseudomonas, phosphate-solubilizing bacillus, nitrogen-fixing bacillus, halophilic bacillus and halomonas into a composite culture medium in proportion, and carrying out stepped sequential fermentation culture under the conditions that the pH value is 7.5 and the temperature is 28 DEG C until the bacterial count is greater than or equal to 1010 CFU / mL. According to the preparation of the fertilizer based on the microbial compound elements and the application of saline-alkali soil treatment, a synergistic system of the compound functional flora, the chelated mineral substances and the intelligent organic carrier is constructed, so that multi-dimensional improvement of saline-alkali soil is realized, the limitation of a traditional single improvement mode is broken through, the salinity and pH value of the soil are effectively reduced, and the soil quality is improved. The soil fertility and biological activity are remarkably improved, the salt stress resistance of crops is enhanced, the effective period is prolonged, and the preparation cost is low.

The invention discloses a fabric antibacterial performance evaluation method and system, and relates to the technical field of antibacterial data processing. The method for evaluating the antibacterial performance of the fabric comprises the following steps: acquiring a bacterial data set after bacterial culture of a to-be-evaluated sample fabric; performing primary evaluation on the to-be-evaluated sample fabric based on the bacterial data set, and screening out primarily-screened sample fabric qualified in primary evaluation; acquiring physical characteristic data of the primarily screened sample fabric, and analyzing and screening a final qualified sample fabric based on the physical characteristic data; the fabric antibacterial evaluation qualification rate is determined based on the ratio of the sample fabric to the to-be-evaluated sample fabric, preliminary screening is performed, and the antibacterial effect of the fabric can be preliminarily determined; the physical characteristic data of the primarily screened sample fabric is obtained, the finally qualified sample fabric is screened out through physical characteristic data analysis, and the fabric antibacterial evaluation qualification rate is determined, so that the problems of incomplete data and insufficient accuracy in the fabric antibacterial effect evaluation process are effectively solved, and the scientificity and objectivity of antibacterial performance evaluation are enhanced.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention relates to the technical field of fermentation engineering, in particular to a method for increasing the yield of bacterial cellulose through blue-green algae. Comprising the following steps: carrying out fermentation by adopting Komagataeibacter xylinus to produce bacterial cellulose, and adding blue-green algae in the fermentation process; the ratio of the total viable count of the gluconacetobacter xylinum to the total viable count of the blue-green algae is (0.3-0.5): 1. According to the research, the problem that a compact film is formed on the surface of a culture medium by primarily generated bacterial cellulose can be remarkably solved by adding the blue-green algae (especially synechocystis) into a gluconacetobacter xylinum fermentation system, the cellulose production efficiency is improved, and the method has important value in the field of fermentation production of the bacterial cellulose.

The invention provides a bacterium number identification method and system based on combination of image identification and deep learning, and relates to the technical field of microbiological detection, the method comprises the following steps: capturing a dyed culture solution picture through a microscope and camera equipment; the preprocessed culture solution picture is recognized based on an image recognition algorithm, a first recognition result of the number of bacteria is obtained, and preprocessing comprises image segmentation, color correction, contrast adjustment and morphological processing; inputting the preprocessed culture solution picture and the corresponding staining information into a deep learning recognition model to obtain a second recognition result of the number of bacteria; if it is detected that the deviation between the first recognition result and the second recognition result is smaller than a preset deviation threshold value, the second recognition result is set as the number of bacteria in the culture solution picture. By implementing the method, the accuracy and efficiency of identification can be improved, errors caused by limitation of a single technology are reduced, and more accurate and efficient bacterium counting is realized.

The invention discloses a tea leaf theanine composition for protecting a central nervous system as well as a preparation method and application thereof, relates to the field of common foods and health-care foods, and aims to solve the problems that most of existing central nervous protection products are single components or are simply compounded, the synergistic effect is weak and the bioavailability is low. The preparation method comprises the following steps: S1, mixing the tea leaf theanine, the emblic leafflower fruit powder, the ginkgo leaf extract, the wolfberry fruit powder and other raw materials according to a specific ratio, and performing high-temperature sterilization and cooling to obtain a fermentation substrate; s2, inoculating a composite activation microbial inoculum composed of plant lactobacillus, lactobacillus acidophilus and lactobacillus reuteri, and carrying out constant-temperature anaerobic fermentation; and S3, freezing and centrifuging at 4 DEG C, concentrating with an RO membrane, and freezing and drying to obtain the composition. The viable count of the complex microbial inoculant reaches 2.0 * 10 < 9 >-3.0 * 10 < 9 > CFU / mL, and the strain ratio and the fermentation process are optimized. The composition has a synergistic effect of multiple components, can efficiently protect nervous centralis, and is suitable for preparing related common food, health food or functional food.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a mandarin fish prefabricated dish detection method and system and a computer readable storage medium. The mandarin fish prefabricated dish detection method comprises the following steps: acquiring a gel strength original value, an elasticity original value, a water activity original value, an RGB image, a temperature value and a bacteria amount original value of a sample; preprocessing the elasticity original value, the moisture activity original value, the RGB image and the bacteria amount original value to form a first feature vector and a second feature vector corresponding to the sample; calculating the first feature vector and the second feature vector by using a preset neural network model to obtain a first qualified weight corresponding to the sample; on the basis of the original gel strength value, the temperature value and a gel strength correction model, calculating a gel correction strength value corresponding to the sample, and on the basis of the gel correction strength value, obtaining a second qualified weight corresponding to the sample; and on the basis of the first qualified weight and the second qualified weight, calculating a qualified coefficient of the sample, and outputting a second signal value that the sample is unqualified.

The subject matter of the present disclosure relates, in various embodiments, to an apparatus, system and method that provides a new and improved ozone treatment chamber that effectively and efficiently disinfects and sanitizes a multitude of target objects using locally generated ozone and humidity. The present invention comprises a housing unit, an ozone generator, a humidity generator, an ozone destructor and a control system for managing same. In alternate embodiments, in addition to the above structures, a heating system is provided to assist with drying if needed and / or to eradicate lice, bedbugs and their eggs. In use, the present invention disinfectants (at least a 3 log 10 or 99.9% reduction in virus titers) and sanitizes (at least a 3 log 10 or 99.9% reduction in bacterial count) a plurality of viruses and bacteria, respectively, thus eliminating the need for harsh chemical liquids.

The invention belongs to the technical field of agricultural fertilizers, and discloses a preparation process and application of a medical stone double-effect organic fertilizer with soil improving and growth promoting functions, the medical stone double-effect organic fertilizer comprises the following components by weight: 20-40 parts of activated medical stone powder with a particle size of less than or equal to 0.1 mm, and activated by a citric acid solution; the decomposed organic material is prepared by mixing livestock and poultry manure and crop straw according to the mass ratio of 2: 1-4: 1 and performing aerobic composting fermentation through a compound microbial agent, and the water content is smaller than or equal to 30%; the microbial agent contains phosphate solubilizing bacteria, nitrogen-fixing bacteria and rhizosphere growth-promoting bacteria, and the effective viable count is greater than or equal to 2 * 10 CFU / g; the auxiliary material comprises bentonite, a polyacrylamide water-retaining agent and a pH (Potential of Hydrogen) regulator, and the proportion of the bentonite is greater than or equal to 30 percent. The adsorption-desorption performance of the medical stone is enhanced by activating the medical stone through citric acid, and a synergistic system of water retention, fertilizer supply and growth promotion is formed by combining decomposed organic materials and functional microorganisms. According to the fertilizer, the stability of a soil aggregate structure can be remarkably improved (increased by 15-20%), the yield increasing rate of crops reaches 20-30%, and 30% of chemical fertilizer dosage is reduced. The method is suitable for acid soil remediation, continuous cropping obstacle treatment and economic crop planting.

The invention relates to the technical field of bacterial detection, in particular to a fluorescent probe, a preparation method and application thereof, and a method for simultaneously detecting bacterial quantity and bacterial drug resistance. The invention provides a fluorescent probe. The fluorescent probe is prepared from the following components: blue carbon quantum dots (BCDs) and a lanthanide coordination compound sensitized luminescent material (Tb-GMP), the invention also provides a method for simultaneously detecting the number of bacteria and the drug resistance of bacteria, and the method is simple to operate, low in cost, less in time consumption and easy to implement, does not need large-scale experimental equipment, and only needs the assistance of an ultraviolet lamp; the visual detection of the bacterial quantity and bacterial drug resistance can be realized through the change of the system color caused by the change of the fluorescence intensity; and a data analysis system and a linear equation can be further utilized for calculation, so that a detection result can be obtained in a short time, the detection efficiency is improved, and the accuracy is high.

The invention relates to the technical field of thallus quantity detection, in particular to a primer pair and a kit for detecting the number of lactobacillus brevis thalli in a postbiotic preparation and application of the primer pair and the kit. The primer pair provided by the invention has the advantages of strong specificity and high amplification efficiency, and can be used for carrying out qPCR quantitative detection on the quantity of lactobacillus brevis in the metastatic preparation. As the traditional flow cytometry and the plate cell counting method are not suitable for detecting the number of thalli in the metagen preparation, the qPCR quantitative method provided by the invention can be used for rapidly and accurately carrying out quantitative analysis on the number of lactobacillus brevis thalli in the metagen in a relatively short time, and the result is compared with a plate counting result; compared with the prior art, the qPCR counting method has the advantages that the detection result difference does not exceed one order of magnitude, counting does not distinguish viable bacteria and dead bacteria, and the qPCR counting method has the advantages of high specificity and high speed and can make up the limitation that a plate counting method is suitable for viable bacteria counting, so that the qPCR counting method provided by the invention has more advantages in the application of metagen counting.

To provide a technique for reducing the count of bacteria involved in mucin decomposition within the intestine, wherein the present invention allows inhibition of growth of intestinal microbes such as Ruminococcus gnavus, which can be involved in mucin degradation; and thus, it becomes possible to reduce mucin degradation within the intestine, thereby preserving the intestinal barrier function, and contributing to the maintenance or enhancement of host health.SOLUTION: Provided is an agent that comprises agaro-oligosaccharides as an active ingredient to inhibit the growth of intestinal bacteria having, in genomic DNA, a NanA gene composed of a DNA sequence having at least 45.6% sequence identity to Sequence No. 1 (NanA gene of Ruminococcus gnavus).SELECTED DRAWING: Figure 1

This invention relates to the field of microbial technology, and particularly to a compound lactic acid bacteria fermentation agent and its application, a method for producing *Anoectochilus roxburghii* fermentation broth, and related products. The compound lactic acid bacteria fermentation agent comprises *Lactobacillus plantarum* BXM2, *Lactobacillus fermentatus* J IAN, and *Lactobacillus paracasei* YYS-69. Fermenting *Anoectochilus roxburghii* broth using this compound fermentation agent results in stronger acid production, higher viable bacterial counts, and significantly improved antioxidant activity, total polyphenol and flavonoid content, and enhanced hypoglycemic activity. Sensory effects are also significantly improved. This compound lactic acid bacteria fermentation agent, when applied to the fermentation of traditional Chinese medicine, can enhance the antioxidant capacity and effective functional component content of the fermentation broth, thereby improving efficacy. It has great application prospects in the field of deep-processed *Anoectochilus roxburghii* products and even other fermented medicinal and edible products.

The invention relates to the technical field of agricultural microorganisms, in particular to paenibacillus brevis KF-B6 and application thereof, in particular to application in prevention and treatment of continuous cropping obstacles of apples. The preservation number of the paenibacillus brevis KF-B6 is CGMCC (China General Microbiological Culture Collection Center) No.32131. The paenibacillus brevis KF-B6 has the compound functions of quickly producing EPS (Expandable Polystyrene) at high yield, producing chitinase at high yield, producing water-soluble and volatile antibacterial substances, decomposing macromolecular organic matters and the like. The application of the bacterium, the microbial agent and the compound microbial fertilizer thereof can effectively improve the number of bacteria in rhizosphere soil, reduce the number of pathogenic fungi, increase the proportion of the bacteria to the fungi and promote the restoration of a soil microbial ecosystem of a continuous cropping obstacle apple orchard; the proportion of water-stable aggregates is increased, the soil organic matter content is increased, the soil enzyme activity is improved, and the soil is improved; the survival rate of planted saplings in the new variety updating process of the continuous cropping obstacle old orchard is increased, the plant root activity and plant growth are promoted, and the apple continuous cropping obstacle is comprehensively relieved and prevented.

The invention provides bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in preparation of a liquid fertilizer. The preservation number of the bacillus amyloliquefaciens is CGMCC (China General Microbiological Culture Collection Center) NO. 31787. The invention also discloses a microbial liquid bacterial fertilizer. The bacterial fertilizer is fermentation liquor containing bacillus amyloliquefaciens with the viable count of 1.0 * 10 < 9 > cfu / mL or more. The invention further discloses a preparation method of the liquid bacterial fertilizer and application of the liquid bacterial fertilizer in agricultural planting. The liquid bacterial fertilizer disclosed by the invention is good in storage stability, and can effectively inhibit plant germs, decompose nutrient substances, promote crop absorption, improve soil micro-ecology, prevent and control plant diseases and improve the yield and quality of crops. And the method is simple to use and environment-friendly, has important significance in promoting agricultural production and sustainable development of land resources, and has wide application prospects in agricultural production.

The application discloses a functional probiotic microencapsulated intestinal shield system and a preparation method and application thereof. A core layer comprises four probiotics, i.e., lactobacillus acidophilus La-14, animal bifidobacterium Bb-12, lactobacillus rhamnosus GG and lactobacillus reuteri DSM 17938, and the weight ratio is 3:4:2:1, and the total viable bacterial count is greater than or equal to 10^10 CFU / g. An inner layer is a targeted release control layer, which is composed of ethyl cellulose, polylactic acid, chitosan and lactose, can swell in a pH 5.5 to 6.5 environment and can dissolve in a pH 6.8 to 7.2 environment. A middle layer is a buffer protection layer, which is composed of calcium carbonate, disodium hydrogen phosphate and sodium alginate, and can maintain the internal pH of the microcapsule at 4.5 to 5.5 in a gastric acid environment. An outer layer is an intelligent response protection layer, which is composed of konjac glucomannan, xanthan gum and gum arabic, and can form a protective film in a gastric acid environment with a pH less than 3.0 within 5 minutes. Through the synergistic protection of the four-layer microcapsule structure, the probiotics can achieve a high survival rate in the gastric acid environment of cats, and can be accurately released at specific positions in the intestinal tract, which is helpful to improve the intestinal health of cats.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention provides a degerming control method, a degerming device and an air conditioner, relates to the technical field of air conditioners, and solves the technical problem of high energy consumption of the degerming device of the air conditioner. The degerming control method comprises the following steps: starting a degerming mode, and presetting a threshold group; acquiring parameters of bacteria in a room; wherein the bacteria parameters comprise the number of bacteria and the concentration of bacteria; comparing the acquired bacteria parameters with a preset threshold group to judge the quality interval of the room; and executing different degerming mode operation levels based on the quality interval of the room. According to the air conditioner, the type, number, concentration and the like of indoor air bacteria are obtained through the sensor, a signal is sent to the main control module of the air conditioner, the main control module controls the heating current according to the signal so as to control the atomization degree and speed of the sterilization liquid and control the rotating speed of the fan and the rotating direction of the fan blades to blow out the atomized sterilization liquid, and the sterilization effect is achieved.

The invention relates to the technical field of microbial technology and disease control, and discloses an ETEC phage and a preparation method thereof, the preservation number of the ETEC phage CQ615 is CGMCC No.46362, and the preservation time is December 30, 2024. According to the scheme, the bacteriophage CQ615 which is separated and purified in sewage by taking ETEC as host bacteria can be used for simultaneously splitting multiple serotypes of escherichia coli and has a relatively wide splitting spectrum; whole genome sequencing is carried out by extracting nucleic acid of the bacteriophage, and data are analyzed to find that the bacteriophage is free of non-virulence factors and drug-resistant factors and can become a potential selection mode for safe prevention and treatment of Escherichia coli; through verification of in-vivo and in-vitro experiments, the bacteriophage CQ615 can effectively reduce the number of ETEC bacteria in milk and lettuce, and can improve the survival rate of greater wax moths infected by ETEC in greater wax moth in-vivo experiments.