The invention provides a gene chip for detecting an important drug resistant gene of pseudomonas aeruginosa and a kit thereof. The gene chip comprises a solid-phase carrier and an oligonucleotide probe fixed on the solid-phase carrier, wherein the probe comprises a DNA fragment or a complementary DNA fragment thereof selected from carb, tem, imp, oxa group I, oxa group II, aac (6')-Ib, aac (6')-II, aac (3')-I, aac (3')-II, aadB and a bacterium 16S rRNA gene. The gene chip and the detecting kit can achieve the aim of simultaneously detecting the 10 drug resistant genes of the pseudomonas aeruginosa, have the characteristics of high pertinence, high specificity, high sensitivity, high detecting speed, high repeatability, and the like and are suitable for a hospital, a disease preventing control mechanism and other clinical laboratories to carry out drug resistant gene detection and drug resistance monitoring on the pseudomonas aeruginosa.