11 results about "Alliin" patented technology

This application relates to an alliinase expression gene, an expression vector for the alliinase expression gene, an alliinase expressed by the alliinase expression gene, an engineered bacterium containing the alliinase expression gene, and the application of the alliinase expression gene. The alliinase expression gene of this invention expresses an alliinase with high activity, reaching an enzyme activity of 173.72±12.89 U / mg, which can effectively increase allicin yield in allicin production and has good industrial application prospects.

The invention provides a synthesis method of S-allyl sulfydryl cysteine (SAMC), S-allyl sulfydryl-glutathione (GSSA) and S-allyl sulfydryl-homocysteine (SAMC). The invention further provides a preparation method of the S-allyl sulfydryl-glutathione (GSSA) and the S-allyl sulfydryl-homocysteine (SAMC). Specifically, allicin generated by reaction of alliin and allinase is taken as a substrate and reacts with cysteine (Cys), glutathione (GSH) and homocysteine (Hcy) to generate SAMC, GSSA and SAMHC. According to the method, high-purity SAMC, high-purity GSSA and high-purity SAMHC can be obtained, and the yield is high.

This invention relates to a cultivation method for increasing the alliin content of garlic and its application, belonging to the field of vegetable cultivation technology. The invention provides a cultivation method for increasing the alliin content of garlic, comprising the following steps: (1) applying potassium sulfate aqueous solution during the garlic seedling stage; (2) applying potassium magnesium sulfate aqueous solution and cysteine ​​aqueous solution during the garlic growth stage; (3) manually removing all flower stalks during the garlic bolting stage, and simultaneously spraying paclobutrazol aqueous solution on the leaves; (4) applying potassium magnesium sulfate aqueous solution and valine aqueous solution during the garlic bulb enlargement stage; (5) spraying cold water onto the leaves before harvesting the garlic bulbs until water droplets remain on the leaves. The cultivation method of this invention is simple to operate, easy to learn and master, and has low input costs. Using this cultivation method to grow garlic can significantly increase the alliin content in garlic, resulting in garlic with an alliin content of 1.24% to 1.34%.

The invention relates to an alliinase expression gene, an expression vector of the alliinase expression gene, alliinase expressed by the alliinase expression gene, engineering bacteria containing the alliinase expression gene and application of the alliinase expression gene. The alliinase expressed by the alliinase expression gene has high activity, the enzyme activity can reach 173.72 + / -12.89 U / mg, the alliinase expression gene can effectively increase the yield of allicin in allicin production, and the alliinase expression gene has a good industrial application prospect.

The invention relates to the technical field of food, health care products and medicines, in particular to a method and a preparation for keeping the activity of allicin. The preparation has a core-shell structure, and comprises an active core containing alliin, alliinase, tocopherol and phytic acid, a pH responsive enteric polymer inner layer wrapping the active core, and a hydrophobic protective outer layer wrapping the inner layer. According to the invention, an on-site preparation and on-site use strategy is designed, that is, alliin and alliinase are respectively protected and are mixed to generate allicin during use, and tocopherol is used as a free radical trapping agent and phytic acid is used as a metal ion chelating agent to carry out immediate protection on allicin generation, so that the problems that allicin is easy to decompose and difficult to store are effectively solved; the method is suitable for preparing health-care foods, medicines and animal feed additives.

This invention relates to alliin derived from garlic, allicin (an enzyme-catalyzed product of alliin), diallyl monosulfide, diallyl disulfide, diallyl trisulfide (degradation products of allicin), dehydrated garlic, and dehydrated garlic with inactivated alliinase, for the prevention and / or treatment of psoriasis.

The application belongs to the technical field of biochemistry and particularly relates to a leek flavor precursor S-hydrocarbyl cysteine sulfoxide extraction method, which comprises the following steps: S1, sampling: selecting fresh and complete leek leaves; S2, grinding and crushing, and adding pre-cooled 0.1 mol / L, pH 6.8 phosphate buffer solution, stirring and dissolving to obtain leek solution; S3, separation of S-hydrocarbyl cysteine sulfoxide and alliinase; S4, extraction: adding petroleum ether to the solution containing S-hydrocarbyl cysteine sulfoxide at a volume ratio of 1:1-3, fully stirring, standing and separating, and then taking the water phase; and S5, column chromatography: first separating by using a cation exchange column, and then separating by using a C18 preparation column to obtain methyl alliin and alliin. The application has the advantages that the extraction rate and purity of methyl alliin and alliin can be effectively improved.

The application discloses a pesticide composition for preventing and treating ginger root rot and application thereof, and belongs to the technical field of chemical pesticides. The pesticide composition for preventing and treating ginger root rot comprises hymexazol, metalaxyl-M and ethylic alliin. The application provides the pesticide composition for preventing and treating ginger root rot, which comprises hymexazol, metalaxyl-M and ethylic alliin. The composition is prepared into a complex fungicide, and the suspension agent can efficiently prevent and treat ginger root rot.

The invention discloses a system for sequential delivery and synthesis of allicin and application thereof, and belongs to the field of drug targeted delivery, the system comprises two units of GISA-A and GISA-B. The GISA-A comprises alliinase, and the GISA-B comprises alliin; wherein the system is configured such that, after administration, the alliinase is administered at a first point in time, the alliin is administered at a second point in time, and the second point in time is later than the first point in time. According to the system, allicin can be synthesized at the targeted part of the intestinal tract to realize precise, efficient and stable antibiosis, and meanwhile, safety and patient tolerance can be taken into account.

The garlic extract compounding device comprises a tank body and a discharging pipe, and a driving shaft is rotationally connected to the middle position in the tank body. The device has the beneficial effects that an inner-layer cooling system and an outer-layer cooling system are arranged, efficient and accurate temperature control is achieved, the product quality stability is greatly improved, the choke ring is installed in the cooling jacket outside the tank body, circulation holes are formed in the surface of the choke ring in a staggered mode, a cooling medium forms a snakelike flowing path, short-circuit flowing is avoided, and it is ensured that the wall face of the tank body is evenly cooled; circulation cavities which are communicated with one another are formed in core stirring assemblies such as the driving shaft, the stirring blades and the scraping plates and matched with one-way flow guide pipes in the stirring blades to form a closed-loop internal cooling loop, heat generated by stirring friction can be directly taken away, and the temperature of materials in the compounding process can be strictly controlled to be smaller than or equal to 25 DEG C through the inner-outer double-layer cooling synergistic effect; the problem of degradation of thermosensitive components such as alliin caused by stirring and heating of traditional equipment is solved, and the retention rate of core components of a product is remarkably increased.