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Preparation process of ajoene

A kind of technology of ajoene and process route, applied in the field of ajoene

Inactive Publication Date: 2010-12-29
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no literature report on the method of preparing ajoene with high yield. The current method can only achieve 30-40% conversion of allicin into ajoene, and the product with higher purity can only be obtained through chromatography.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 100 grams of garlic, add 100 grams of water, use shear stirring or a colloid mill to grind into a slurry under an ice bath, maintain the ice bath, stir and mix for 1 hour, add 800 ml of pre-cooled acetone (0 ~ 5 ℃), stir and mix for 30 10 minutes, filtered to remove the solid residue to obtain a clear solution, to which 400 grams of anhydrous magnesium sulfate was added, stirred and mixed for 4 hours at a temperature of 0 to 15 ° C, and then filtered to remove solids, and the filtrate was iced at 50 to 60 ° C. It was maintained under the bath for 3-4 hours, followed by TLC until complete conversion was achieved. Use a rotary evaporator to evaporate the water-soluble organic solvent acetone at less than 60 ° C to obtain a turbid liquid containing oil droplets, add ethyl acetate for extraction (50ml*3) three times, combine the extracts, add 5 grams of anhydrous sodium sulfate to dry 2 After 1 hour, the ethyl acetate was distilled off with a rotary evaporator under a ...

Embodiment 2

[0019] Alliin was synthesized according to the method reported in the literature (Stoll, H. Experimentia, 1950, 6:330).

[0020] The natural alliin was prepared according to the method reported in the literature (Huang Cedar, Food Science, 2004, 25(11): 200-203).

[0021] Take 100 ml of water, add 4 grams of synthetic alliin or natural alliin to it, add and Cl2, pyrrolidin hydrochloride, such as: EDTA, Mg2+, Fe2+, pyridoxine hydrochloride, etc. as enzyme activity accelerators, after fully stirring Dissolve and add 100 grams of garlic, carry out enzymatic reaction according to the method of Example 1 and convert it into product ahoene. TLC analysis was performed with n-butanol:methanol:acetic acid:water (4:1:1:0.5) as the developing solvent and ninhydrin as the chromogenic reagent to follow the enzymatic conversion. The analysis showed that both synthetic alliin and natural alliin could be completely enzymatically converted. The obtained product was analyzed by high performan...

example 3

[0023] Take 100 grams of garlic and 100 grams of pure water, grind it into a slurry with a shear mixer or colloid under an ice bath, remove the solid precipitate by centrifugation with a low temperature centrifuge, or quickly filter it with a 200-mesh filter cloth to obtain a slightly mixed solution, Transfer to the ice bath, add enzyme activity accelerator, stabilizer and 4 grams of alliin according to the method of example 2, carry out enzymatic reaction and be converted into ahoene, also can complete enzymatic reaction and realize ahoene. preparation.

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PUM

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Abstract

The invention relates to a preparation process of ajoene, comprising the steps of: converting natural alliin or synthesized alliin into allicin in a water solution by utilizing alliinase in garlic, adding a water-soluble organic solvent, such as alcohol, ketone, ether and the like, to remove partial impurities so as to prepare an allicin solution, then adding a dehydrating agent for moderate dehydration, converting 90% of allicin into the ajoene at moderate temperature, and evaporating the solvent to obtain an ajoene product with the purity of 90%. The process is easy to operate and is suitable to scale preparation of the ajoene products.

Description

technical field [0001] The invention relates to the technical field of biochemistry and natural products, and relates to a process for preparing allicin by biological methods and converting it into ahoene by chemical methods. Background technique [0002] Ahopene is an active substance isolated from garlic. This substance has a wide range of physiological activities. It is a new type of antithrombotic agent, bacteriostatic agent and lipase inhibitor. It also has anti-AIDS and malignant tumors. certain effect, so ahoene is a compelling active substance. Over the years, people have been looking for a suitable method to prepare ahoene. As early as the 1980s, Eric Block in the United States successfully prepared ahoene from garlic. The method chopped garlic into small pieces and soaked them in methanol for three times. The filtrate was concentrated and then extracted with ether. The ether extract was concentrated and dissolved in methanol again. It was placed in a refrigerator ...

Claims

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Application Information

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IPC IPC(8): C12P11/00C07C323/65C07C319/22C07C319/28
CPCY02P20/54
Inventor 张相年赵树进严浩成李超蒋琳兰
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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