33 results about "Ajoene" patented technology

The invention relates to a preparation method of ajoene, wherein the preparation method is simple and convenient in operation, low in pollution and less in energy consumption. The method comprises the following process steps: grinding bulb of garlic, and adding in a CO2 supercritical extractor to obtain CO2 supercritical extract; and adding the extract in a molecular distillator, performing three-stage molecular distillation, and collecting distillate, thus obtaining the ajoene. The ajoene prepared by the method provided by the invention has high product purity, and the industrial production can be realized easily.

The present invention provides a composition for removing a hangover. In particular, the present invention discloses a method of using ajoene as an alcohol dehydrogenase inhibitor based on the fact that ajoene strongly inhibits alcohol dehydrogenase (ADH) activity. A composition for removing a hangover comprising ajoene suppresses the over-production of acetaldehyde during alcohol metabolism to maintain acetaldehyde concentration in blood at a normal level that can be degraded in the body and eliminated therefrom through normal metabolic and secretory pathways to thereby prevent the hangover after consumption of alcohol, and a method of preparing the same, are also provided. The present invention also provides a novel use of ajoene, which is a natural substance obtained by purification of garlic, and a composition for removing a hangover comprising ajoene, which is harmless to the body and strongly inhibits ADH activity, thereby showing very excellent relieving effect on the hangover.

The present invention relates to the compound (E,Z)-ajoene of formula (1) for use in treatment of bacterial infections. Another aspect of the present invention is a composition comprising (E,Z)-ajoene of formula (1) and at least one antibiotic. Yet another aspect of the invention relates to a method for manufacturing (E,Z) ajoene of formula (1) wherein the conformation of the internal C═C— bond can be either E or Z or a mixture thereof, said method comprising reacting allicin of formula (3) with an acid in the presence of a solvent to provide (E,Z ajoene) of formula (1) as defined above. Yet another aspect of the invention is (E,Z)-ajoene of formula 1 obtainable by the method described above.

The invention discloses a method for promoting conversion of alliin in garlic into ajoene. The method comprises the following steps of (1) crushing garlic so as to obtain garlic granules; (2) adding vegetable oil to the garlic granules, and performing uniform stirring so as to obtain a garlic-vegetable oil mixture; (3) placing the mixture in an iron pan, and performing stir-frying at high temperature; and (4) filtering the stir-fried mixture so as to obtain garlic granules of which the ajoene content is increased and a vegetable oil product which is rich in ajoene. The method disclosed by theinvention has the advantages that the technology is simple, and the stability is good; the crushed garlic and an appropriate amount of vegetable oil are mixed, the alliin is converted into the ajoenethrough catalysis under the high temperature condition, the content of the ajoene in the treated garlic granules is increased by 4 times or above, and the content of the ajoene in oil is 20 times or above of that of untreated garlic.

A single-particle nano-allicin microcapsule, which is composed of a single-particle garlic active ingredient and a wall material embedded outside it, the wall material is N-LOK modified starch or β-CD, and the active ingredients include allicin, allicin, Ajoene, Selenium and Germanium. Preparation steps: put the washed and purified garlic raw material in a container, then add ethanol, dichloromethane or water solvent with a concentration of 70-100%, put it into a multi-dimensional microwave environment for multi-dimensional microwave-induced extraction, and the extraction temperature is 40-50°C , the extraction pressure is 0.06-0.12Mpa, and its active ingredients are extracted into the solvent; after being filtered, it is sent to the concentration tank for concentration under reduced pressure to obtain a mixed extract containing the active ingredients of garlic; the active ingredients are obtained by condensation; the concentrated solution is added N-LOK modified starch or β-CD, stirred at low temperature to make a mixture liquid, and then sent to the ring-shaped nano-jet atomizer for atomization and drying, so as to obtain single-particle nano-garlicin microcapsules and make them into powder, tablets or Capsule dosage form; it has the advantages of high extraction rate and basically no damage to plant active ingredients.

A process for the preparation of ajoene, and of ajoene having a relatively high purity which may be obtained by the process. This invention also relates to processes for the preparation of allicin, and to a process for freeze concentrating allicin.

The invention discloses a preparation method of natural ajoene, which comprises the following steps: (1) breaking and juicing garlic powder, and separating to form a product A, (2) mixing the product A and a solvent for natural fermentation, and separating to form a product B, (3) mixing the product B and vegetable oil to form a product C, (4) performing solvent extraction on the product C to form an extract as a product D, (5) performing decompression concentration and column chromatography separation on the product D, eluting with a solution containing an eluent to form an eluent as a product E, and (6) performing decompression on the product E to remove the solvent, and obtaining a high-purity natural ajoene product. According to the method, allinase contained in garlic is subjected to fermentation, extraction, chromatography separation to form the high-purity natural ajoene sample; a technology is simple and stable; conversion of alliin into ajoene can be promoted; ajoene can be extracted and separated efficiently; the method has the advantages that the method is adapted industrial production, and the purity of ajoene is high.

The invention relates to a method for extracting and separating ajoene from black garlic. The method comprises four steps: 1, smashing medicinal materials; 2, performing supercritical CO2 extraction; 3, performing molecular distillation separation; 4, purifying preparative liquid phase. According to the method, the black garlic is extracted by a supercritical CO2 extraction device, the extractant is separated and enriched by a molecular distillation device, and the enriched sample is dissolved by a solvent to directly perform preparative liquid phase purification; the ajoene purity in a finished product prepared by the method is larger than 70%, and the transfer rate is 80% or more; the method is reasonable and practicable, has the advantages of being good in repeatability and stability and is suitable for large-scale industrial production.

The present invention relates to a process for producing ajoene from allicin via a polymeric substrate, comprising the steps of heating allicin or an allicin solution at a predetermined temperature range such that at least a portion of the allicin is converted to ajoene to form an ajoene solution; and separating the ajoene from the ajoene solution; wherein at least one of the heating or separating steps is conducted via a polymeric substrate. The process for producing ajoene from allicin via a polymeric substrate further comprises a step of retaining the allicin or the allicin solution on the polymeric substrate before the heating step is conducted within or out of the polymeric substrate. The present invention also relates to ajoene or a composition comprising ajoene obtained by the process thereof.

The invention belongs to the technical field of separation and purification of effective ingredients in traditional Chinese medicines and discloses a method for separating and purifying ajoene in a garlic extract through molecular distillation. According to the method, ajoene is rapidly extracted from garlic with an ultrasonic technology and then separated and purified with a molecular distillation separation and purification technology, and a high-purity ajoene product is obtained. Solvents, water and low-boiling-point impurities left in the garlic extract are removed through level-I distillation in a molecular distillation device, ajoene in level-I fractions is separated and purified through level-II distillation, and the ajoene product with the purity higher than 88% is obtained. The prepared product has no solvent residues, is high in purity and has light color. The method has the advantages that operation is simple and convenient, the preparation cost is low, the separation effect is good, the product purity is high, the utilization rate of raw material medicine residues is high and the like, and is suitable for industrial production.

The invention belongs to the technical field of extraction and separation of effective components of traditional Chinese medicines, and discloses an extraction and separation method of ajoene in garlic. According to the method, by utilizing an ultrasonic technology, the garlic is preliminarily treated by taking ethyl alcohol as a solvent, and the lower-content ajoene in the garlic is extracted and enriched from raw materials within a short time, so as to improve the efficiency for further extraction and separation; secondary extraction, refinement and separation are performed by utilizing the characteristic that CO2 has a selective dissolving capacity for substances with different polarities such as the ajoene under a super-critical state, and the purity of an ajoene product obtained finally is greater than 80 percent. The novel method provided by the invention overcomes the defects of the existing preparation method, has the advantages of short production period, environmental friendliness, high ajoene purity, high utilization rate of raw material medicine residue and the like, and can be used for large-batch industrial production.

The invention relates to a garlic ajoene reference substance preparation method. The preparation method comprises the following steps: 1) crushing garlic, adding a solvent, performing high- pressure homogeneous extraction, filtering the material to obtain an extract; 2) adding the solvent in the extract for extraction, standing and layering the material, taking an upper layer of the solution to obtain an extract; 3) performing vacuum concentration on the extract, through silica-gel column chromatography, eluting the solvent to obtain an eluate; 4) performing vacuum concentration on the eluate, through separating and purifying a liquid phase to obtain the ajoene-rich eluate; and 5) performing vacuum concentration on the eluate to obtain the ajoene reference substance. Each index of four spectrums and purity determination of the ajoene reference substance can be accorded with a national standard. The method is simple and stable, is used for preparing the garlic ajoene reference substance, and provides the technical support for deep research of ajoene.

The present invention relates to an ajoene preparation method, which comprises: (1) crushing garlic, and adding an extraction solution to mix so as to obtain a product A; (2) extracting the product A, and then carrying out a filtration and / or centrifugation treatment to obtain a solution, ie., product B; (3) removing the solvent from the product B, and adding vegetable oil to mix so as to obtain a product C; (4) adding the product C to an adsorbent to mix so as to obtain a product D; (5) eluting the product D with an eluant-containing solution to obtain a product E; and (6) removing the solvent from the product E so as to obtain the product containing the high concentration ajoene. According to the new method of the present invention, the defects in the existing preparation method are improved, and the new method has advantages of adaptability to industrial production, short production cycle, environmental protection, high ajoene purity, high utilization of raw material residue, and the like.