33 results about "Ajoene" patented technology

The present invention relates to the use of extracts and compounds of plants from the Allium genus for the food and agrifood industry consisting of being used as food preservatives for humans and animals, plants being harvested or post-harvest plants, as well as for environmental disinfection, using thiosulfinates, thiosulfonates, ajoene, vinyl dithiins and products resulting from the decomposition of thiosulfinates.

The invention relates to a preparation process of ajoene, comprising the steps of: converting natural alliin or synthesized alliin into allicin in a water solution by utilizing alliinase in garlic, adding a water-soluble organic solvent, such as alcohol, ketone, ether and the like, to remove partial impurities so as to prepare an allicin solution, then adding a dehydrating agent for moderate dehydration, converting 90% of allicin into the ajoene at moderate temperature, and evaporating the solvent to obtain an ajoene product with the purity of 90%. The process is easy to operate and is suitable to scale preparation of the ajoene products.

The present invention relates to an ajoene preparation method, which comprises: (1) crushing garlic, and adding an extraction solution to mix so as to obtain a product A; (2) extracting the product A, and then carrying out a filtration and / or centrifugation treatment to obtain a solution, ie., product B; (3) removing the solvent from the product B, and adding vegetable oil to mix so as to obtain a product C; (4) adding the product C to an adsorbent to mix so as to obtain a product D; (5) eluting the product D with an eluant-containing solution to obtain a product E; and (6) removing the solvent from the product E so as to obtain the product containing the high concentration ajoene. According to the new method of the present invention, the defects in the existing preparation method are improved, and the new method has advantages of adaptability to industrial production, short production cycle, environmental protection, high ajoene purity, high utilization of raw material residue, and the like.

The invention discloses a preparation method of a composite rich in ajoene. The preparation method comprises the following steps of transforming natural alliin extracted from garlic into diallyl thiosulfinate by taking alliinase as a catalyst; carrying out membrane concentration, extraction, separation and enrichment, and then reacting and purifying to obtain a high-content ajoene product. The preparation method disclosed by the invention has the advantages of available process raw materials, easily controlled production process conditions and high product purity.

The invention belongs to the technical field of deep processing of garlic and particularly relates to a process method and a preparation method for synthesizing ajoene from allicin. The process method comprises the steps of extracting allinase, purifying allinase, immobilizing allinase, preparing allicin, synthesizing ajoene, and purifying ajoene. According to the process method, allinase is immobilized by virtue of an enzyme immobilization method and is converted into allicin through catalysis in a bioreactor, the reaction specificity is good, the catalytic efficiency is high, and a high-purity allicin product can be obtained; by aiming at characteristics of cleavage reaction of allicin, allicin is complexed by virtue of a hydrophilous complex in a complex catalysis reaction manner, so that the stability of allicin is improved; allicin is decomplexed in a reaction process and can timely react so as to generate ajoene, so that a large number of side reactions are avoided.

The invention relates to a preparation method of ajoene, wherein the preparation method is simple and convenient in operation, low in pollution and less in energy consumption. The method comprises the following process steps: grinding bulb of garlic, and adding in a CO2 supercritical extractor to obtain CO2 supercritical extract; and adding the extract in a molecular distillator, performing three-stage molecular distillation, and collecting distillate, thus obtaining the ajoene. The ajoene prepared by the method provided by the invention has high product purity, and the industrial production can be realized easily.

The invention discloses a preparation method for high-purity natural garlic ajoene. The preparation method comprises the following steps: (1) crushing garlic, adding a first solvent, and naturally fermenting to obtain a product A; (2) mixing the product A with a second solvent, extracting by ultrasound to obtain a product B; (3) extracting and separating the product B by a supercritical CO2 device, to obtain a product C; (4) purifying the product C by the industrial chromatographic separation, and collecting ajoene-enriched eluant which is a product D; (5) vacuum-concentrating the product D to remove the solvents, to obtain a natural garlic ajoene product of which the purity is greater than 98%. The provided method is capable of using allinase contained in the garlic to ferment, extracting the ajoene by using the ultrasonic wave, and purifying the ajoene by using the supercritical CO2 extraction technology, and finally using the industrial chromatographic technology to prepare the high-purity ajoene product. The technology is simple and stable, the purity of the ajoene is high, and the technology is capable of providing the foundation for preparing an ajoene standard substance, and developing a new drug.

The invention relates to a garlic ajoene for curing the cardio-cerebral vascular disease and the cancer, and the preparation method thereof, and belongs to the technical field of the extraction of the herbal medicine. The ajoene of the invention for curing the cardio-cerebral vascular disease is extracted from fresh garlic, and obtained by the chromatography. The ajoene can prevent the thrombus from forming, distend the coronary artery, enhance the immune function, and inhibit the growth of cancer cells. The ajoene can be made into an oral preparation or injection preparation, which is used to treat the cardio-cerebral vascular diseases of arteriosclerosis and cardiac infarction, etc., prevent and cure the cancer and the tumour.

The present invention relates to the compound (E,Z)-ajoene of formula (1) for use in treatment of bacterial infections. Another aspect of the present invention is a composition comprising (E,Z)-ajoene of formula (1) and at least one antibiotic. Yet another aspect of the invention relates to a method for manufacturing (E,Z) ajoene of formula (1) wherein the conformation of the internal C═C— bond can be either E or Z or a mixture thereof, said method comprising reacting allicin of formula (3) with an acid in the presence of a solvent to provide (E,Z ajoene) of formula (1) as defined above. Yet another aspect of the invention is (E,Z)-ajoene of formula 1 obtainable by the method described above.

The present invention provides a composition for removing a hangover. In particular, the present invention discloses a method of using ajoene as an alcohol dehydrogenase inhibitor based on the fact that ajoene strongly inhibits alcohol dehydrogenase (ADH) activity. A composition for removing a hangover comprising ajoene suppresses the over-production of acetaldehyde during alcohol metabolism to maintain acetaldehyde concentration in blood at a normal level that can be degraded in the body and eliminated therefrom through normal metabolic and secretory pathways to thereby prevent the hangover after consumption of alcohol, and a method of preparing the same, are also provided. The present invention also provides a novel use of ajoene, which is a natural substance obtained by purification of garlic, and a composition for removing a hangover comprising ajoene, which is harmless to the body and strongly inhibits ADH activity, thereby showing very excellent relieving effect on the hangover.

The present invention relates to the compound (E,Z)-ajoene of formula (1) for use in treatment of bacterial infections. Another aspect of the present invention is a composition comprising (E,Z)-ajoene of formula (1) and at least one antibiotic. Yet another aspect of the invention relates to a method for manufacturing (E,Z) ajoene of formula (1) wherein the conformation of the internal C═C— bond can be either E or Z or a mixture thereof, said method comprising reacting allicin of formula (3) with an acid in the presence of a solvent to provide (E,Z ajoene) of formula (1) as defined above. Yet another aspect of the invention is (E,Z)-ajoene of formula 1 obtainable by the method described above.

The invention discloses a method for preparing a fermentation product rich in natural ajoene. The method includes the following steps that (1) medicinal materials are smashed; (2) crushed garlic and plant oil are ground; (3) fermentation is carried out; (4) garlic residues are removed, and the fermentation product is obtained; the method is characterized in that after fresh garlic is smashed by a certain granularity, the product and vegetable oil are ground to obtain a garlic-vegetable oil mixture, the mixture is fermented to obtain the fermentation product rich in natural ajoene, and the content of the ajoene is 0.120% or above. According to the method, allinase of garlic is used for fermentation, the technology is simple and stable, an organic reagent is not used in the preparing process, the preparing cost is saved, and the method has the advantages of being capable of being suitable for industrialization production, good in environment, high in ajoene content and the like.

The invention provides an ajoene microcapsule powder product and its preparation method and use. The ajoene microcapsule powder product can be processed to form a plurality of dosage forms so that an application range is widened and properties and stability are good. The preparation method comprises carrying out extraction on garlicin-containing raw materials through water, filtering the extract to obtain a garlicin aqueous solution, extracting garlicin in the garlicin aqueous solution through an organic solvent, collecting the organic solvent phase, removing water, drying the garlicin to obtain garlicin-containing garlic oil, preparing a beta-cyclodextrin aqueous solution, embedding the garlicin-containing garlic oil with the beta-cyclodextrin aqueous solution to obtain a beta-cyclodextrin suspension, drying and crushing the beta-cyclodextrin suspension, and sieving the powder to obtain the ajoene microcapsule powder product. The preparation method can be used for ajoene microcapsule powder product preparation.

The invention discloses a method for promoting conversion of alliin in garlic into ajoene. The method comprises the following steps of (1) crushing garlic so as to obtain garlic granules; (2) adding vegetable oil to the garlic granules, and performing uniform stirring so as to obtain a garlic-vegetable oil mixture; (3) placing the mixture in an iron pan, and performing stir-frying at high temperature; and (4) filtering the stir-fried mixture so as to obtain garlic granules of which the ajoene content is increased and a vegetable oil product which is rich in ajoene. The method disclosed by theinvention has the advantages that the technology is simple, and the stability is good; the crushed garlic and an appropriate amount of vegetable oil are mixed, the alliin is converted into the ajoenethrough catalysis under the high temperature condition, the content of the ajoene in the treated garlic granules is increased by 4 times or above, and the content of the ajoene in oil is 20 times or above of that of untreated garlic.

A single-particle nano-allicin microcapsule, which is composed of a single-particle garlic active ingredient and a wall material embedded outside it, the wall material is N-LOK modified starch or β-CD, and the active ingredients include allicin, allicin, Ajoene, Selenium and Germanium. Preparation steps: put the washed and purified garlic raw material in a container, then add ethanol, dichloromethane or water solvent with a concentration of 70-100%, put it into a multi-dimensional microwave environment for multi-dimensional microwave-induced extraction, and the extraction temperature is 40-50°C , the extraction pressure is 0.06-0.12Mpa, and its active ingredients are extracted into the solvent; after being filtered, it is sent to the concentration tank for concentration under reduced pressure to obtain a mixed extract containing the active ingredients of garlic; the active ingredients are obtained by condensation; the concentrated solution is added N-LOK modified starch or β-CD, stirred at low temperature to make a mixture liquid, and then sent to the ring-shaped nano-jet atomizer for atomization and drying, so as to obtain single-particle nano-garlicin microcapsules and make them into powder, tablets or Capsule dosage form; it has the advantages of high extraction rate and basically no damage to plant active ingredients.

The invention relates to the field of insecticides, in particular to a selenium-enriched crop insecticide. It consists of the following raw materials in parts by weight: 30-40 parts of tobacco leaf extract, 2-5 parts of ajoene, 5-20 parts of amino acids, 10-15 parts of onion juice, 10-15 parts of tea polyphenols, 3 parts of carbamate ~5 parts, 3~5 parts of diallyl disulfide. Adopting the present invention can effectively kill aphids, affect the normal nerve conduction of insects and cause death, diallyl disulfide has anthelmintic effect, and ajoene, tobacco leaf extract and onion juice have a killing effect on aphids. The composition of the invention has the advantages of obvious synergism, delay resistance and the like.

A process for the preparation of ajoene, and of ajoene having a relatively high purity which may be obtained by the process. This invention also relates to processes for the preparation of allicin, and to a process for freeze concentrating allicin.

The present invention discloses black garlic and Chinese pear fruit paste, and mainly consists of the following raw materials in percentages by weight: black garlic powder 5-10%, diced Chinese pears 15-25% and citric acid 0.3-1%, xylitol 5-15%, sodium carboxymethyl cellulose 0.1-0.5%, beta-cyclodextrin 0.2-0.5% and the balance water. The fruit paste makes full use of the green antiseptic function of the black garlic and the antioxidant function of ajoene enriched in black garlic fermentation, thereby reducing the use of preservatives and antioxidants in the fruit paste production process; meanwhile the sweet and sour flavor of the black garlic and the sweet taste of the Chinese pears complement each other so that the produced fruit paste is sweet and sour in taste and pleasant, and has effects in resisting cancer, resisting aging, promoting saliva secretion, moisturizing dryness syndrome, clearing heat, reducing phlegm, etc.

The invention discloses a preparation method of natural ajoene, which comprises the following steps: (1) breaking and juicing garlic powder, and separating to form a product A, (2) mixing the product A and a solvent for natural fermentation, and separating to form a product B, (3) mixing the product B and vegetable oil to form a product C, (4) performing solvent extraction on the product C to form an extract as a product D, (5) performing decompression concentration and column chromatography separation on the product D, eluting with a solution containing an eluent to form an eluent as a product E, and (6) performing decompression on the product E to remove the solvent, and obtaining a high-purity natural ajoene product. According to the method, allinase contained in garlic is subjected to fermentation, extraction, chromatography separation to form the high-purity natural ajoene sample; a technology is simple and stable; conversion of alliin into ajoene can be promoted; ajoene can be extracted and separated efficiently; the method has the advantages that the method is adapted industrial production, and the purity of ajoene is high.

The invention discloses a novel broad-spectrum insecticide with long efficacy. The novel broad-spectrum insecticide comprises, by weight, 3-7 parts of tobacco leaf extract liquid, 0.4-0.9 part of toosendanin, 5-9 parts of plant ash soak liquid, 2-6 parts of ailanthus altissima juice, 0.3-0.6 part of tetramethrin, 3-7 parts of surfactants, 0.2-0.5 part of indolebutyric acid, 0.3-0.8 part of gibberellin, 0.1-0.4 part of diafenthiuron, 0.3-0.7 part of toluene, 4-8 parts of moringa oleifera leaf liquid, 2-5 parts of emulsifiers, 0.2-0.8 part of carbamate, 0.1-0.3 part of ajoene, 0.3-0.7 part of matrine, 0.2-0.7 part of triazophos, 2-5 parts of penetrating agents, 2-5 parts of omethoate, 0.2-0.5 part of tea polyphenols, other 0.3-0.7 part of carbamate, 2-5 parts of stabilizers, 0.7-1.2 parts ofco-solvents and 3-7 parts of organic solvents. The novel broad-spectrum insecticide has the advantages that aphids can be effectively killed by the novel broad-spectrum insecticide, and excellent insecticidal effects can be realized by a low dose of the novel broad-spectrum insecticide; the novel broad-spectrum insecticide is long in lasting period, can be applied once at the intervals of 30-40 days and is low in destructiveness on ecological systems, and the like.

The invention discloses a vegetable insecticide. The vegetable insecticide is prepared from the raw materials in parts by weight: 20-30 parts of a tobacco extracting solution, 18-26 parts of twigs andleavesf of manyflower supplejack, 16-22 parts of dahurian patrinia herbs, 12-18 parts of vernonia anthelmintica, 14-18 parts of fructus cnidii, 8-12 parts of oxymatrine, 10-20 parts of japanese buttercup herbs, 6-8 parts of ajoene, 12-17 parts of elm bark, 3-5 parts of thiamethoxam, 7-9 parts of a dispersing agent and 8-12 parts of an emulgator. The vegetable insecticide can efficiently kill insects, is easy to decompose without residues, has no harm to crops and does not pollute the environment.

The invention discloses a method for extracting and separating ajoene from garlic, and belongs to the technical field of deep processing of agricultural products. The method disclosed by the invention comprises the following steps: pre-treating garlic cloves under the conditions of vacuum, pressure of more than 400MPa and time of more than 10min; the pretreated garlic cloves are crushed at the temperature of-4 DEG C or below and then subjected to an enzymatic reaction under the conditions of 100-200 MPa and 25-35 DEG C, and minced garlic is obtained; extracting the minced garlic with subcritical fluid to obtain garlic oil; and sequentially carrying out molecular distillation and liquid phase purification on the garlic oil to obtain ajoene. According to the method, alliinase is passivated through ultrahigh pressure to extract alliin, then alliinase liquid is prepared, then alliinase in garlic is activated through ultrahigh pressure, the enzymatic reaction speed can be increased, more allicin is generated, and then ajoene with the higher content is generated; and finally, carrying out ultrahigh pressure regulation and control, subcritical fluid extraction, molecular distillation and preparative liquid phase purification to obtain a high-yield and high-purity ajoene product.

The invention relates to an ajoene capsule which is prepared from the following components in parts by weight: 80-200 parts of ajoene, 300-600 parts of vegetable oil. The ajoene capsule has the beneficial effects that the ajoene is mixed with the vegetable oil to prepare a capsule, so that a user can relatively easily take the ajoene capsule; and the ajoene capsule has a plurality of healthcare effects, such as an anti-thrombosis effect, an anti-tumor effect, an anti-inflammatory effect, resistance against cardiovascular and cerebrovascular diseases with the symptoms of hypertension, hyperlipidemia and thick blood.

The invention relates to a method for extracting and separating ajoene from black garlic. The method comprises four steps: 1, smashing medicinal materials; 2, performing supercritical CO2 extraction; 3, performing molecular distillation separation; 4, purifying preparative liquid phase. According to the method, the black garlic is extracted by a supercritical CO2 extraction device, the extractant is separated and enriched by a molecular distillation device, and the enriched sample is dissolved by a solvent to directly perform preparative liquid phase purification; the ajoene purity in a finished product prepared by the method is larger than 70%, and the transfer rate is 80% or more; the method is reasonable and practicable, has the advantages of being good in repeatability and stability and is suitable for large-scale industrial production.

The present invention relates to a process for producing ajoene from allicin via a polymeric substrate, comprising the steps of heating allicin or an allicin solution at a predetermined temperature range such that at least a portion of the allicin is converted to ajoene to form an ajoene solution; and separating the ajoene from the ajoene solution; wherein at least one of the heating or separating steps is conducted via a polymeric substrate. The process for producing ajoene from allicin via a polymeric substrate further comprises a step of retaining the allicin or the allicin solution on the polymeric substrate before the heating step is conducted within or out of the polymeric substrate. The present invention also relates to ajoene or a composition comprising ajoene obtained by the process thereof.

The invention belongs to the technical field of separation and purification of effective ingredients in traditional Chinese medicines and discloses a method for separating and purifying ajoene in a garlic extract through molecular distillation. According to the method, ajoene is rapidly extracted from garlic with an ultrasonic technology and then separated and purified with a molecular distillation separation and purification technology, and a high-purity ajoene product is obtained. Solvents, water and low-boiling-point impurities left in the garlic extract are removed through level-I distillation in a molecular distillation device, ajoene in level-I fractions is separated and purified through level-II distillation, and the ajoene product with the purity higher than 88% is obtained. The prepared product has no solvent residues, is high in purity and has light color. The method has the advantages that operation is simple and convenient, the preparation cost is low, the separation effect is good, the product purity is high, the utilization rate of raw material medicine residues is high and the like, and is suitable for industrial production.

The invention belongs to the technical field of extraction and separation of effective components of traditional Chinese medicines, and discloses an extraction and separation method of ajoene in garlic. According to the method, by utilizing an ultrasonic technology, the garlic is preliminarily treated by taking ethyl alcohol as a solvent, and the lower-content ajoene in the garlic is extracted and enriched from raw materials within a short time, so as to improve the efficiency for further extraction and separation; secondary extraction, refinement and separation are performed by utilizing the characteristic that CO2 has a selective dissolving capacity for substances with different polarities such as the ajoene under a super-critical state, and the purity of an ajoene product obtained finally is greater than 80 percent. The novel method provided by the invention overcomes the defects of the existing preparation method, has the advantages of short production period, environmental friendliness, high ajoene purity, high utilization rate of raw material medicine residue and the like, and can be used for large-batch industrial production.

The invention relates to a garlic ajoene reference substance preparation method. The preparation method comprises the following steps: 1) crushing garlic, adding a solvent, performing high- pressure homogeneous extraction, filtering the material to obtain an extract; 2) adding the solvent in the extract for extraction, standing and layering the material, taking an upper layer of the solution to obtain an extract; 3) performing vacuum concentration on the extract, through silica-gel column chromatography, eluting the solvent to obtain an eluate; 4) performing vacuum concentration on the eluate, through separating and purifying a liquid phase to obtain the ajoene-rich eluate; and 5) performing vacuum concentration on the eluate to obtain the ajoene reference substance. Each index of four spectrums and purity determination of the ajoene reference substance can be accorded with a national standard. The method is simple and stable, is used for preparing the garlic ajoene reference substance, and provides the technical support for deep research of ajoene.