Process method for synthesizing ajoene from allicin

A process method, technology of allicin, applied in directions such as hydrogenated polysulfide/polysulfide preparation, organic chemistry, etc., which are fixed on/in organic carrier, can solve complexity, unfavorable ajoene synthesis, side reaction and other problems, to achieve high catalytic efficiency, avoid a large number of side reactions, and achieve the effect of high yield

Inactive Publication Date: 2017-07-18
山东晨隆晟世生物科技有限公司
View PDF7 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These decomposition products lead to the complexity of the conversion of allicin into ajoene, and a large number of side reactions occur, which is not conducive to the synthesis of ajoene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of processing method of allicin synthetic ajoene, is made up of the following steps:

[0028] (1) Allinase extraction: Grind the garlic added with allinase extract with a colloid mill to obtain garlic pulp, and centrifuge to separate the supernatant; at 0°C, add ammonium sulfate to the supernatant, and stir while adding, Then separate the second clear liquid; then add polyethylene glycol to the second clear liquid, stir while adding, centrifuge to sort out the precipitate, redissolve, and obtain the supernatant after centrifugation; the present invention immobilizes the immobilized enzyme according to According to the requirements of the process and the specificity of industrial enzymes, establish an enzyme purification process suitable for large-scale applications. The enzyme activity yield and enzyme specific activity were used as indicators to investigate the components of the extract solution in the allinase purification process. According to the enzymatic pr...

Embodiment 2

[0035] A kind of processing method of allicin synthetic ajoene, is made up of the following steps:

[0036] (1) Allinase extraction: Grind the garlic added with allinase extract with a colloid mill to obtain garlic pulp, and centrifuge to separate the supernatant; at -5°C, add ammonium sulfate to the supernatant, and stir while adding , and then separate the second supernatant; then add polyethylene glycol to the second supernatant, stir while adding, centrifuge to separate out the precipitate, redissolve, and obtain the supernatant after centrifugation; the present invention immobilizes According to the requirements of the chemical process and the specificity of industrial enzymes, establish an enzyme purification process suitable for large-scale applications. The enzyme activity yield and enzyme specific activity were used as indicators to investigate the components of the extract solution in the allinase purification process. According to the enzymatic properties of garlic ...

Embodiment 3

[0043] A kind of processing method of allicin synthetic ajoene, is made up of the following steps:

[0044] (1) Allinase extraction: Grind the garlic added with allinase extract with a colloid mill to obtain garlic pulp, and centrifuge to separate the supernatant; at 5°C, add ammonium sulfate to the supernatant, and stir while adding, Then separate the second clear liquid; then add polyethylene glycol to the second clear liquid, stir while adding, centrifuge to sort out the precipitate, redissolve, and obtain the supernatant after centrifugation; the present invention immobilizes the immobilized enzyme according to According to the requirements of the process and the specificity of industrial enzymes, establish an enzyme purification process suitable for large-scale applications. The enzyme activity yield and enzyme specific activity were used as indicators to investigate the components of the extract solution in the allinase purification process. According to the enzymatic pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of deep processing of garlic and particularly relates to a process method and a preparation method for synthesizing ajoene from allicin. The process method comprises the steps of extracting allinase, purifying allinase, immobilizing allinase, preparing allicin, synthesizing ajoene, and purifying ajoene. According to the process method, allinase is immobilized by virtue of an enzyme immobilization method and is converted into allicin through catalysis in a bioreactor, the reaction specificity is good, the catalytic efficiency is high, and a high-purity allicin product can be obtained; by aiming at characteristics of cleavage reaction of allicin, allicin is complexed by virtue of a hydrophilous complex in a complex catalysis reaction manner, so that the stability of allicin is improved; allicin is decomplexed in a reaction process and can timely react so as to generate ajoene, so that a large number of side reactions are avoided.

Description

technical field [0001] The invention belongs to the technical field of garlic deep processing, and in particular relates to a process method for synthesizing ajoene from allicin and a preparation method thereof. Background technique [0002] Allicin (allicin, garlicin) is a trithioallyl ether compound that naturally exists in the bulbs of the liliaceous plant garlic. It has antibacterial effect on a variety of Gram-positive and negative bacteria, and has antibacterial effects on bacilli (shigella, typhoid bacillus, Escherichia coli, pertussis bacillus), fungi (candida albicans, cryptococcus, aspergillus fumigatus), viruses (cytomegalovirus ), amoeba, trichomonas vaginalis, pinworm, etc. all have inhibitory and killing effects, especially for intestinal bacteria such as Escherichia coli and Shigella. [0003] However, allicin is extremely unstable and will decompose at room temperature. As an active ingredient derived from garlic, ajoene is more stable than allicin and does...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P11/00C12N11/08C12N11/04C07C319/22C07C323/65
CPCC07C319/22C12N11/04C12N11/08C12P11/00C07C323/65
Inventor 薛乃峰刘福锦智鲁侯仰元朱香平
Owner 山东晨隆晟世生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap