3results about How to "Strong response specificity" patented technology

The invention discloses an interactive response layered superlattice nanoprobe as well as a synthesis method and application thereof, and belongs to the technical field of nanomaterials and biosensing. The nanoprobe is synthesized through a ligand regulation and control self-assembly strategy, specifically, a platinum source, n-caprylic acid and molybdenum hexacarbonyl are mixed and heated for a reaction, and after the reaction is finished, aftertreatment is conducted to obtain the interactive response layered superlattice nanoprobe. The nanoprobe has a highly-ordered layered superlattice structure, and can generate synergistic change of structure and catalytic activity under different H2O2 concentrations, so that specific interactive response to H2O2 is realized. The nanoprobe is controllable in synthesis process and stable in structure, the interactive response performance of the nanoprobe provides core material support for threshold type detection of biomarkers, the technical problems that a traditional nanomaterial is single in response to substrate concentration change and insufficient in sensitivity are solved, and the nanoprobe has important application value in the fields of biosensing, clinical detection and the like.

This application discloses a nonspecific peroxidase CsaUPO derived from *Cladorrhinum samala*, whose amino acid sequence is shown in SEQ ID NO:1. This enzyme efficiently catalyzes the 4,5β-epoxidation of testosterone to produce 4,5β-epoxidized testosterone, achieving a conversion rate of 98% and a separation yield of 90%, without producing hydroxylation byproducts, and exhibiting excellent regioselectivity. This application also discloses the gene encoding CsaUPO, the recombinant plasmid pPICZA-CsaUPO containing this gene, a recombinant Pichia pastoris engineered strain, and its application in steroid epoxidation. The nonspecific peroxidase CsaUPO disclosed in this application possesses advantages such as high catalytic efficiency, specific regioselectivity, a simple reaction system, and environmental friendliness, providing a new enzymatic resource for the site-directed epoxidation modification of steroid drugs.

The invention relates to the technical field of polymer chemical synthesis, in particular to a preparation method of methoxy polyethylene glycol methacrylate or methoxy polyethylene glycol acrylate. A two-step synthesis method is adopted and comprises the following steps: firstly, carrying out ethoxylation reaction on methacrylic acid or acrylic acid and ethylene oxide in the presence of a catalyst to generate a methacrylic acid polyethylene glycol ester intermediate or an acrylic acid polyethylene glycol ester intermediate; and carrying out end capping on the intermediate, alkali and halomethane through a Williamson ether synthesis reaction to obtain high-purity methoxy polyethylene glycol methacrylate or methoxy polyethylene glycol acrylate. The invention overcomes the problems of high reaction temperature, low conversion rate, insufficient product purity and easy polymerization of methacrylate or acrylate in the traditional transesterification method, and overcomes the defects of long synthetic route steps and high cost in the prior art. The method has the advantages of mild process conditions, controllable molecular weight and high product purity, and is suitable for the fields of biomedical materials, cosmetics, polymer emulsifiers and the like.