6results about How to "Strong response specificity" patented technology

The present invention provides recombinant Bacillus subtilis BLCY-013 and its application in preparing mannose with glucose as raw material, belonging to the field of biotechnology. The recombinant Bacillus subtilis BLCY-013 of the present invention was stored in the General Microbiological Center of China Microbiological Culture Collection Administration Committee on November 25, 2024, with an address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the strain collection number is CGMCC No. 32760; the present invention uses glucose as a raw material, and prepares mannose by recombinant Bacillus subtilis enzyme reaction, does not use chemical reagents, has strong reaction specificity, and is significantly reduced in raw material cost compared with the traditional method of preparing with fructose as a substrate; the unreacted glucose in the preparation process is recycled, and the raw material utilization rate is high; evaporation crystallization is adopted, and the prepared mannose is mannose without crystal water by controlling the crystallization parameters, which has good fluidity, weak moisture absorption, and is not easy to agglomerate.

The present invention relates to the field of catalyst technology, and in particular to a method for synthesizing 2,5-dimethyl-2,4-hexadiene from isobutyraldehyde catalyzed by phosphoric acid-modified Zn / HZSM-5, with isobutylene (IB) / isobutyraldehyde (IBA) as reactants, and a Prince condensation reaction is carried out under the conditions of a phosphoric acid-modified Zn-loaded HZSM-5 molecular sieve catalyst to obtain the obtained product. The invention has the advantages that the process is simple, the energy consumption is low, the safety is high, the cost is low, the pollution is small, and large-scale production is possible; the prepared product 2,5-dimethyl-2,4-hexadiene content can reach 60%, which meets the requirements of industrial products; the reaction specificity is strong, there are no other by-products except water, the catalyst is easy to separate, can be reused, and is environmentally friendly and energy-saving.

The invention discloses an interactive response layered superlattice nanoprobe as well as a synthesis method and application thereof, and belongs to the technical field of nanomaterials and biosensing. The nanoprobe is synthesized through a ligand regulation and control self-assembly strategy, specifically, a platinum source, n-caprylic acid and molybdenum hexacarbonyl are mixed and heated for a reaction, and after the reaction is finished, aftertreatment is conducted to obtain the interactive response layered superlattice nanoprobe. The nanoprobe has a highly-ordered layered superlattice structure, and can generate synergistic change of structure and catalytic activity under different H2O2 concentrations, so that specific interactive response to H2O2 is realized. The nanoprobe is controllable in synthesis process and stable in structure, the interactive response performance of the nanoprobe provides core material support for threshold type detection of biomarkers, the technical problems that a traditional nanomaterial is single in response to substrate concentration change and insufficient in sensitivity are solved, and the nanoprobe has important application value in the fields of biosensing, clinical detection and the like.

The invention discloses a cyclohexadiene derivative and a preparation method thereof. According to the method, the chemical synthesis blank of the tetrodotoxin intermediate 10 is filled, and the cyclohexadiene derivative is obtained by taking cheap and easily available D-ribose as a raw material through the steps of hydroxyl protection, alkylation, oxidation, addition, cyclization, elimination, deprotection and the like. The preparation method is high in atom utilization degree and high in reaction specificity of each step, compared with enzyme catalysis, the research and development cost is reduced, complex separation and purification operation is avoided, and the preparation method has industrial amplification prospects.

This application discloses a nonspecific peroxidase CsaUPO derived from *Cladorrhinum samala*, whose amino acid sequence is shown in SEQ ID NO:1. This enzyme efficiently catalyzes the 4,5β-epoxidation of testosterone to produce 4,5β-epoxidized testosterone, achieving a conversion rate of 98% and a separation yield of 90%, without producing hydroxylation byproducts, and exhibiting excellent regioselectivity. This application also discloses the gene encoding CsaUPO, the recombinant plasmid pPICZA-CsaUPO containing this gene, a recombinant Pichia pastoris engineered strain, and its application in steroid epoxidation. The nonspecific peroxidase CsaUPO disclosed in this application possesses advantages such as high catalytic efficiency, specific regioselectivity, a simple reaction system, and environmental friendliness, providing a new enzymatic resource for the site-directed epoxidation modification of steroid drugs.

The invention relates to the technical field of polymer chemical synthesis, in particular to a preparation method of methoxy polyethylene glycol methacrylate or methoxy polyethylene glycol acrylate. A two-step synthesis method is adopted and comprises the following steps: firstly, carrying out ethoxylation reaction on methacrylic acid or acrylic acid and ethylene oxide in the presence of a catalyst to generate a methacrylic acid polyethylene glycol ester intermediate or an acrylic acid polyethylene glycol ester intermediate; and carrying out end capping on the intermediate, alkali and halomethane through a Williamson ether synthesis reaction to obtain high-purity methoxy polyethylene glycol methacrylate or methoxy polyethylene glycol acrylate. The invention overcomes the problems of high reaction temperature, low conversion rate, insufficient product purity and easy polymerization of methacrylate or acrylate in the traditional transesterification method, and overcomes the defects of long synthetic route steps and high cost in the prior art. The method has the advantages of mild process conditions, controllable molecular weight and high product purity, and is suitable for the fields of biomedical materials, cosmetics, polymer emulsifiers and the like.