The invention relates to a method for synthesis of Etimicin sulfate intermediate 3, 2', 6'-tri-N-acetyl gentamicin Cla. The method consists of the following steps of: under certain temperature, reacting gentamicin Cla with zinc acetate in a methanol solvent to generate a complex, cooling the reaction solution and adding triethylamine, adding a tetrahydrofuran solution of acetic anhydride dropwisely for an acylation reaction, carrying out liquid phase tracking, and adding a sodium oxalate solution after completion of the reaction, filtering a zinc oxalate precipitate, delivering the filtrate to ion exchange resin, first conducting washing with pure water, then conducting washing with acid of certain concentration, adjusting the pH of the acid washed liquid to 9, performing concentration, adding ethanol to separate out a solid, implementing filtration, and freeze-drying the filtrate, thus obtaining the 3, 2', 6'-tri-N-acetyl gentamicin Cla.