Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pharmaceutical composition of etimicin sulfate and application of pharmaceutical composition in biomedicine

A technology of etimicin sulfate and its composition, which is applied in the field of biomedicine, can solve the problems that have not yet been reported on etimicin sulfate acute gouty arthritis, and achieve the effect of improving the therapeutic effect

Inactive Publication Date: 2016-07-20
周俭
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there has been no report on the correlation between etimicin sulfate and its pharmaceutical composition and acute gouty arthritis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical composition of etimicin sulfate and application of pharmaceutical composition in biomedicine
  • Pharmaceutical composition of etimicin sulfate and application of pharmaceutical composition in biomedicine
  • Pharmaceutical composition of etimicin sulfate and application of pharmaceutical composition in biomedicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0021] Separation method: (a) Pulverize Dajinhua (3kg), extract with 75% ethanol under heat reflux (20L×3 times), combine the extracts, concentrate until no alcohol smell (4L), and successively wash with petroleum ether (4L×3 times), ethyl acetate (4L × 3 times) and water-saturated n-butanol (4L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step ( a) Use AB-8 type macroporous resin to remove impurities from the n-butanol extract, first elute 8 column volumes with 6% ethanol, then elute 8 column volumes with 70% ethanol, collect 70% eluate, reduce Concentrate under pressure to get 70% ethanol elution concentrate; (c) in step (b), 70% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 50:1 (8 column volumes), 25:1 successively (8 column volumes), 15:1 (8 column volumes) and 5:1...

Embodiment 2

[0024] Embodiment 2: pharmacological action

[0025] In this example, sodium urate was used to prepare an acute gouty arthritis model, and the test joint circumference and prostaglandin E in the joint cavity tissue were observed before and after drug treatment. 2 (PGE), tumor necrosis factor-α (TNF-α) content and the number of leukocytes in joint effusion.

[0026] 1. Materials and methods

[0027] 1.1 Animals

[0028] SD rats, male, clean grade, weighing 200-250 g, were provided by the Experimental Animal Center of Guangdong Medical College. Tested after normal feeding in the laboratory for 3 days.

[0029] 1.2 Reagents and samples

[0030] Etimicin sulfate was purchased from China National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Sodium urate (supplied by Sigma), prostaglandin E 2 (PGE 2 ) radioimmunoassay kit (provided by Beijing East Asia Institute of Immunology...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a pharmaceutical composition of etimicin sulfate and application of the pharmaceutical composition in biomedicine. The pharmaceutical composition comprises the etimicin sulfate and a natural product compound (I) novel in structure; when in single action, the etimicin sulfate and the compound (I) have therapeutical effect in acute gouty arthritis; when the etimicin sulfate and the compound (I) are in combined action, therapeutical effect in acute gouty arthritis is further improved, so that the pharmaceutical composition can be developed into drugs for treating acute gouty arthritis; compared with the prior art, the pharmaceutical composition has outstanding substantive features and significant improvement.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of etimicin sulfate, in particular to a pharmaceutical composition of etimicin sulfate and its application in biomedicine. Background technique [0002] Etimicin sulfate is an aminoglycoside antibiotic, which has a strong killing effect on bacteria in the quiescent phase and is an antibacterial drug in the quiescent phase. Adverse reactions include ototoxicity, nephrotoxicity, neuromuscular blockade, blood picture changes, increased liver enzymes, flora imbalance, and superinfection. [0003] So far, there is no report on the correlation between etimicin sulfate and its pharmaceutical composition and acute gouty arthritis. Contents of the invention [0004] The purpose of the present invention is to provide a pharmaceutical composition of etimicin sulfate, containing etimicin sulfate and a novel natural product in the pharmaceutical composition, etimicin sulfate and t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J71/00A61K31/7036A61K31/585A61P29/00A61P19/02A61P19/06
CPCC07J71/001A61K31/585A61K31/7036
Inventor 周俭
Owner 周俭
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com