Yokenella sp. and application thereof in preparing alpha, beta-unsaturated enol and aromatic alcohol

Abstract

The invention provides a novel bacterial strain-Yokenella sp. WZY002 and an application thereof in preparing alpha, beta-unsaturated olefine aldehyde (ketone) through regioselective reduction and aromatic alcohol through aromatic aldehyde (ketone) reduction. The bacterial strain is preserved in the China general microbiological culture collection center (CCTCC); the address is 430072, Wuhan University, Wuhan, China; the preservation number is CCTCC No: M2013099; the preservation date is March 22, 2013. The novel bacterial strain has the main beneficial effects of high regioselectivity, high stereoselectivity and high enzymatic activity, regioselective reduction of the alpha, beta-unsaturated enol is catalyzed so as to obtain various alpha, beta-unsaturated olefine aldehydes, and reduction of the aromatic aldehyde (ketone) can also be catalyze so as to obtain the aromatic alcohol. The bacterial strain is served as a biocatalyst and has high regioselectivity, high stereoselectivity and strong catalytic activity, the catalytic reaction does not need to add coenzyme, the reaction temperature is moderate, and the novel bacterial strain has a relatively high application value for industrial production.

Description

(1) Technical field [0001] The present invention relates to a new bacterial strain - Yokenella sp. WZY002, and its regioselective reduction of α, β-unsaturated alkenal or α, β-unsaturated enone to prepare α, β-unsaturated Applications in enols, asymmetric reduction of aromatic ketones to prepare chiral aromatic alcohols, and reduction of aromatic aldehydes to prepare aromatic alcohols. (2) Background technology [0002] α,β-unsaturated enols are very important intermediates in organic synthesis (including drug synthesis), such as cinnamyl alcohol, limonyl alcohol, crotyl alcohol, etc., which are widely used in the production of spices, drugs and other fine chemicals, and are often used as It is used as food aroma additive, aroma blending agent and pharmaceutical intermediate, etc., with high economic value. [0003] The selective hydrogenation of α,β-unsaturated alkenals (ketones) is a key step in the synthesis of fragrances and pharmaceutical intermediates. Since the acti...

AI Technical Summary

Problems solved by technology

Since the activation energy of the C=C bond is lower than that of the C=O bond in thermodynamics, and the C=C bond is more active than the C=O bond in kinetics, and under the action of general chemical catalysts, α, β-unsaturated enaldehyde The main product of (ketone) is mostly saturated aldehyde (ketone), and the yield of more valuable product α, β-unsaturated enol is lower

However, there is no report on the preparation of α,β-unsaturated enols catalyzed by Yorkella microorganisms.

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