Method for synthesizing natural alpha-tocofecol tocopherol acid succinate by using lipase

Abstract

A method for synthesizing natural alpha-tocopherol succinic acid monoester using lipase pertains to the field of biological chemicals, which is characterized in that the preparation method includes: adding the substrate of natural alpha-tocopherol and succinic anhydride at molar ratio of 1:1-1:8 in an organic solvent, adding lipase to start the reaction, oscillating or mixing reaction 20-72 hours under the conditions of 20-45 DEG C, wherein, the conversion rate of the alpha-tocopherol is 80-98.39%. Compared with the prior industry process in which esterification is performed at high-temperature by using high toxicity chemical catalyst under, the method has advantages of mild reaction conditions (room temperature, atmospheric pressure), low energy consumption, non-toxic catalyst, specificity, high reaction efficiency fewer accessory substances, and the like.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method for synthesizing natural alpha-tocopherol succinic acid monoester by using lipase to catalyze the reaction of natural alpha-tocopherol and succinic anhydride in an organic solvent. Background technique [0002] Vitamin E, including tocopherol and tocotrienol, is a kind of natural antioxidant and an essential natural nutrient in the human body. It has significant anti-oxidation, eliminates free radicals in the body, eliminates sexual dysfunction, promotes blood circulation, Prevent premature aging, prevent cancer, improve the body's immunity and other functions. Vitamin E includes four tocopherol homologues and four tocotrienol homologues, among which α-tocopherol has the strongest biological activity. At present, α-tocopherol can be extracted from natural plants or chemically synthesized. The biological activity of natural α-tocopherol is 2 to 3 times that of syntheti...

AI Technical Summary

Problems solved by technology

(1) The reaction requires high temperature and high energy consumption

(2) The specificity of the catalyst is poor, resulting in many side reactions, dark color of the product, difficulties in separation and purification, etc., and the quality of the final product is poor

(3) The high temperature of the reaction process makes vitamin E easy to oxidize and deteriorate, so nitrogen protection is required, which increases the production cost and the complexity of the reactor

The preparation process of the substrate unsaturated carboxylic acid ester is complicated and the price is relatively high

Moreover, additional water-absorbing agents need to be added during the reaction process to increase the conversion rate, which increases production costs and is not conducive to subsequent separation and purification operations.

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