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Preparation method of composite rich in ajoene

A kind of technology of ajoene and composition, applied in the field of composition preparation, can solve the problems of unstable diallyl thiosulfinate, low product yield, complicated purification process and the like

Inactive Publication Date: 2014-01-22
四川省荣桓科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diallyl thiosulfinate is extremely unstable at room temperature and decomposes into different substances under different conditions. On the one hand, under mild conditions, diallyl thiosulfinate produces ajoene Class (ajoenes) or cyclization reaction to produce vinyldithiins (vinyldithiins); on the other hand, under severe conditions, such as distillation to form diallyl disulfide, diallyl trisulfide and other Thioether compounds (sulfides), the activity is greatly reduced
The above methods all have the problems of complex purification process and low product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of diallyl thiosulfinate.

[0022] Weigh 100g of alliin with a mass percentage of 50%, dissolve it in 10L of buffer solution with pH=6.5, add 0.1L of allinase solution (allinase activity 640U / mL), stir and react at 37°C for 60 minutes, and react The concentration of diallyl thiosulfinate in the back measurement solution was 1.6g / L, and the yield was 72.1%.

Embodiment 2

[0023] Example 2 Preparation of diallyl thiosulfinate.

[0024] The purified allinase was embedded in chitosan and cross-linked with glutaraldehyde, and its activity was determined to be 83U / g. Take 2kg of immobilized enzyme beads and put them into a jacketed glass column (Φ50*1000cm), and the jacket is circulated with hot water at 37°C; take 2kg of alliin with a mass percentage of 50% and dissolve it in 120L of pH=6.5 buffer In the process, the reaction solution passes through the enzyme column at a flow rate of 3L / h, and after completion, it is washed with water until the effluent has no garlic smell. 153 L of the reaction solution was collected, and the concentration of diallyl thiosulfinate was measured to be 5.26 g / L, and the yield was 84.48%.

Embodiment 3

[0025] Example 3 Purification of diallylthiosulfinate.

[0026] Take 50L of the reaction solution in Example 2, concentrate it to 5L with a nanofiltration membrane with a molecular weight cut-off (MWCO) of 200D, extract 4 times with 5L ether, collect the ether solution, dehydrate it with anhydrous sodium sulfate, and concentrate in vacuo to obtain purified diene Propyl thiosulfinate 168.4g, the purity is 64.2%.

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Abstract

The invention discloses a preparation method of a composite rich in ajoene. The preparation method comprises the following steps of transforming natural alliin extracted from garlic into diallyl thiosulfinate by taking alliinase as a catalyst; carrying out membrane concentration, extraction, separation and enrichment, and then reacting and purifying to obtain a high-content ajoene product. The preparation method disclosed by the invention has the advantages of available process raw materials, easily controlled production process conditions and high product purity.

Description

technical field [0001] The invention relates to a preparation method of a composition rich in ajoene, in particular to a preparation method involving high-content trans-ajoene. Background technique [0002] Ajoene, also known as allicene, has two structures of cis (Z-ajoene) and trans (E-ajoene), and is a biological substance isolated from the extract obtained by soaking broken garlic bulbs in vegetable oil The active ingredient is a product obtained by slowly decomposing diallyl thiosulfinate under mild conditions. Ajoene, as a natural and stable active ingredient derived from garlic, has been paid more and more attention by the scientific and technological circles, and research reports on its pharmacological and physiological functions are also emerging. Blood pressure, detoxification, anti-cancer, anti-oxidation, anti-aging, cell-mediated immunity of organisms, humoral immune regulation and other processes can all play an important role. The main active substance in gar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P11/00C07C319/22C07C323/65
Inventor 刘福锦孙波黄稳包塔娜曾艳丽任建波
Owner 四川省荣桓科技有限责任公司
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