6 results about "Alliinase" patented technology

This application relates to an alliinase expression gene, an expression vector for the alliinase expression gene, an alliinase expressed by the alliinase expression gene, an engineered bacterium containing the alliinase expression gene, and the application of the alliinase expression gene. The alliinase expression gene of this invention expresses an alliinase with high activity, reaching an enzyme activity of 173.72±12.89 U / mg, which can effectively increase allicin yield in allicin production and has good industrial application prospects.

The invention provides a synthesis method of S-allyl sulfydryl cysteine (SAMC), S-allyl sulfydryl-glutathione (GSSA) and S-allyl sulfydryl-homocysteine (SAMC). The invention further provides a preparation method of the S-allyl sulfydryl-glutathione (GSSA) and the S-allyl sulfydryl-homocysteine (SAMC). Specifically, allicin generated by reaction of alliin and allinase is taken as a substrate and reacts with cysteine (Cys), glutathione (GSH) and homocysteine (Hcy) to generate SAMC, GSSA and SAMHC. According to the method, high-purity SAMC, high-purity GSSA and high-purity SAMHC can be obtained, and the yield is high.

The invention relates to an alliinase expression gene, an expression vector of the alliinase expression gene, alliinase expressed by the alliinase expression gene, engineering bacteria containing the alliinase expression gene and application of the alliinase expression gene. The alliinase expressed by the alliinase expression gene has high activity, the enzyme activity can reach 173.72 + / -12.89 U / mg, the alliinase expression gene can effectively increase the yield of allicin in allicin production, and the alliinase expression gene has a good industrial application prospect.

The invention relates to the technical field of food, health care products and medicines, in particular to a method and a preparation for keeping the activity of allicin. The preparation has a core-shell structure, and comprises an active core containing alliin, alliinase, tocopherol and phytic acid, a pH responsive enteric polymer inner layer wrapping the active core, and a hydrophobic protective outer layer wrapping the inner layer. According to the invention, an on-site preparation and on-site use strategy is designed, that is, alliin and alliinase are respectively protected and are mixed to generate allicin during use, and tocopherol is used as a free radical trapping agent and phytic acid is used as a metal ion chelating agent to carry out immediate protection on allicin generation, so that the problems that allicin is easy to decompose and difficult to store are effectively solved; the method is suitable for preparing health-care foods, medicines and animal feed additives.

This invention relates to alliin derived from garlic, allicin (an enzyme-catalyzed product of alliin), diallyl monosulfide, diallyl disulfide, diallyl trisulfide (degradation products of allicin), dehydrated garlic, and dehydrated garlic with inactivated alliinase, for the prevention and / or treatment of psoriasis.

The invention discloses a system for sequential delivery and synthesis of allicin and application thereof, and belongs to the field of drug targeted delivery, the system comprises two units of GISA-A and GISA-B. The GISA-A comprises alliinase, and the GISA-B comprises alliin; wherein the system is configured such that, after administration, the alliinase is administered at a first point in time, the alliin is administered at a second point in time, and the second point in time is later than the first point in time. According to the system, allicin can be synthesized at the targeted part of the intestinal tract to realize precise, efficient and stable antibiosis, and meanwhile, safety and patient tolerance can be taken into account.