40 results about "Aspergillosis" patented technology

The invention discloses a Pichia manshurica for controlling postharvest diseases of oranges and grapes, and belongs to the field of biological control of postharvest diseases of fruits. A strain is identified as Pichia manshurica, and is named as Pichia manshurica Y4, and the preservation number of the Pichia manshurica Y4 is CGMCC 2.5415. When the Pichia manshurica is used, the Pichia manshurica is activated, cultured and centrifuged to obtain thalli, and the thalli are diluted with sterile water to prepare a thallus suspension with the concentration of 1.0*10<8>/mL. The suspension is sprinkled to the exocuticle of the fruits through using a watering pot, the fruits are air-dried, the dried fruits are put in a plastic basket, the plastic basket is sealed with a preservative film, and the sealed fruits are stored under room temperature conditions. The incidence of orange Penicillium digitatum, grape Aspergillus niger and gray molds is substantially reduced after treatment with the Pichia manshurica. The Pichia manshurica can substitute chemical bactericides to prevent and control postharvest diseases of oranges and grapes, avoids harms of the chemical bactericides to people, and has substantial economic and social benefits.

The invention discloses a hanseniaspora vineae BY19 for preventing and controlling postharvest diseases of fruits and vegetables and with wide antibacterial spectrum and stable effect and a use methodthereof. The collection number of the strain in China General Microbiological Culture Collection Center is CGMCC No. 14903. The use method of the hanseniaspora vineae comprises the following steps of: activating the strain, carrying out fermentation culture by using YPD, centrifuging, and preparing thalli into 1*108 cell/mL of bacterial suspension by using sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citruses or cherry tomatoes and the like into the bacterial suspension, soaking for 30 seconds, then taking out, and carrying out air drying; puttinginto a fresh-keeping box, and storing at normal temperature. The hanseniaspora vineae strain disclosed by the invention has the beneficial effects that penicilliosis and botrytis cinerea of the apples, the penicilliosis and the botrytis cinerea of the pears, botrytis, aspergillosis, black spot, fusaridiosis and anthracnose of the grapes, the botrytis of the strawberries and the botrytis and the aspergillosis of the cherry tomatoes can be controlled simultaneously, so that the loss caused by the postharvest diseases is reduced and the application prospect is good.

Oligonucleotides and methods for using these oligonucleotides in the detection of Aspergillus fumigatus are disclosed. Aspergillus fumigatus is the causative agent for medical conditions including invasive aspergillosis. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (i.e., the Cyp51A protein) of Aspergillus fumigatus. The oligonucleotides of the invention include forward primers and reverse primers which in combination are capable of priming the synthesis of amplicons specific to cyp51A in polymerase chain reactions using nucleic acids isolated from Aspergillus fumigatus as templates. The oligonucleotides of the invention also include probes capable of detecting these cyp51A-specific amplicons. Thus, a biological sample is tested for the presence of Aspergillus fumigatus by isolating nucleic acid from the sample, conducting a polymerase chain reaction in a mixture containing this nucleic acid and these forward and reverse primers, and then determining, using an oligonucleotide probe, whether an amplicon is produced in the mixture, wherein detection of the amplicon indicates the presence of Aspergillus fumigatus in the sample. The oligonucleotides of the invention also include primers for nucleotide sequencing reactions to determine whether an isolate of Aspergillus fumigatus is more tolerant than wild-type Aspergillus fumigatus to a triazole, which is a compound commonly used as an antifungal drug. Specifically, a strand of a cyp51A-specific amplicon is at least partially sequenced using a nucleotide sequencing primer in a primer extension reaction. Identification of mutations giving rise to amino acid substitutions at positions 54, 138, 220, and 448 of the amino acid sequence of the wild-type Cyp51A protein indicates that the isolate of Aspergillus fumigatus from which the amplicon is derived exhibits decreased susceptibility to at least one triazole.

The invention discloses a primer and a probe of invasive mucor pathogenic bacteria, an implementation method and a detection system thereof. The method comprises the following steps of: comparing whole genome data of all strains of the obtained invasive mucor pathogenic bacteria to obtain a plurality of conserved genes of the invasive mucor pathogenic bacteria; and designing a universal primer and a probe of the invasive mucor pathogenic bacteria with specificity meeting the requirement and a species-specific primer and a probe of all strains of the invasive mucor pathogenic bacteria by utilizing the plurality of conserved genes of the invasive mucor pathogenic bacteria obtained by whole genome data comparison. By utilizing the primer and the probe designed by the invention, the sensitivity and the specificity of detection of the invasive mucor pathogenic bacteria can be improved, and various strains including aspergillus fumigatus, aspergillus flavus, aspergillus terreus, aspergillus niger and aspergillus nidulans can be detected; and the primer and the probe are suitable for different clinical samples such as BALF and FFPE tissues and exudates (pus).

The invention provides a saccharifying enzyme produced by fermenting draff mixed fluid and a method for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish, which relates to a method for producing saccharifying enzyme and a way for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish. The method for producing the saccharifying enzyme comprises the following procedures: I. mixing draff fluid with water, adding solid draff; II. disinfecting, adding aspergillosis fluid to ferment. The method for producing alcohol: a. mixing kitchen and food rubbish with waste water from a dining hall or tap water; b. adding saccharifying enzyme produced by above method, inoculating saccharomycetes, then fermenting under an anaerobic atmosphere; c. distilling the alcohol. The invention provides nitrous substances necessary for microbes to grow by using draff fluid and solid draff, which can act as traditional enzyme-producing culture medium. The invention can improve the activity of the saccharifying enzyme, and the produced saccharifying enzyme can be directly used for alcohol fermenting kitchen and food rubbish without purifying and separating, this can not only reduce the production cost for alcohol, but also change waste into treasure, improve the added value of kitchen and food rubbish, and avoid pollution from draff and draff fluid on environment.

The present invention provides monoclonal antibodies specific for members of the Aspergillus genus and uses thereof for increasing the survival rate of a subject infected with an Aspergillus species. The present invention also provides conjugates of alliinase with monoclonal antibodies specific for members of the Aspergillus genus that preserve the ability of the antibody to recognize the fungus as well as the activity of the enzyme to produce cytotoxic molecules of allicin from the substrate alliin. The invention further provides pharmaceutical compositions comprising the conjugates of the mAbs of the invention and alliinase and uses thereof in combination with the substrate alliin in the treatment of diseases cause by Aspergillus species, in particular invasive aspergillosis.

The invention discloses a strain BY25 of Pichia deserticola and a use method and applications thereof. The strain has a wide antibacterial spectrum, is used to control diseases of picked vegetables and fruits, and has a stable effect. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC, and the preservation number is CGMCC No. 14906. The use method of Pichia deserticola comprises following steps: activating strains, using YPD to carry out fermentation culture, carrying out centrifugation, preparing bacterial suspension (1*10<8> cells/mL) from bacteria andsterile water; soaking fruits and vegetables (the apple, pear, grape, strawberry, orange, cherry tomato, etc.) in the bacterial suspension for 30 seconds, drying fruits and vegetables in the air, placing the fruits and vegetables in a fresh-keeping box, and storing the fruits and vegetables at a room temperature. The Pichia deserticola strain can control blue mold, gray mold, and black spot of theapple, blue mode and gray mold of the pear fruit, gray mold, aspergillosis, black spot, pink rot, and phoma disease of the grape, grey mold and aspergillosis of the straw berry, grey mold of orange,and grey mold and aspergillosis of the cherry tomato, reduces the loss caused by diseases after picking, and has a good application prospect.

The invention discloses a Pichia galeiformis bacterial strain BY27 used for control of postharvest diseases of fruits and vegetables and with broad antibacterial spectrum and stable effect and an application method thereof. The bacterial strain is preserved in CGMCC with the preservation number of CGMCC No.14907. The application method of the pichia pastoris comprises the following steps: activating the bacterial strain, carrying out fermental cultivation with YPD, centrifuging, and preparing bacterium suspension with the concentration of 1*10<8> cells/mL from thalli with sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citrus and cherry tomatoes into the bacterium suspension, soaking for 30 seconds, then taking out, and carrying out air drying; andputting into a preservation box, and storing at room temperature. The Pichia galeiformis bacterial strain disclosed by the invention can control penicilliosis, botrytis and black spot of the apples,penicilliosis and botrytis of pear fruits, botrytis, aspergillosis, black spot, fusariosis and cephalothecium roseum of the grapes, botrytis and aspergillosis of the strawberries, penicilliosis of thecitrus as well as botrytis and aspergillosis of the cherry tomatoes at the same time, and loss caused by the postharvest diseases is reduced, so that the Pichia galeiformis bacterial strain disclosedby the invention has a good application prospect.

The invention provides an Aspergillus flavus prevention and control composition containing perillaldehyde and a calcium ion chelating agent EDTA, and an application thereof. The composition comprisesa component A and a component B, the component A is perillaldehyde, the component B is the calcium ion chelating agent EDTA, the concentration of perillaldehyde is 100-1000 [mu]g/L, and the concentration of the calcium ion chelating agent EDTA is 200-1000 [mu]g/mL. The composition has a strong inhibiting effect on peanut Aspergillus flavus, and can be used for preparing medicines for preventing and controlling peanut Aspergillus flavus. The perillaldehyde is natural, has no toxic or side effect, does not easily cause pathogenic microorganisms to generate pesticide resistance, and can be used for preventing and controlling peanut Aspergillus flavus; and the composition is safe to human and livestock, and is a low-toxicity effective antibacterial biological pesticide. The perillaldehyde andthe calcium ion chelating agent EDTA have huge potential as the Aspergillus flavus bactericide.

The invention discloses traditional Chinese medicine ferment capable of improving the livestock and poultry meat quality and a preparation method of the traditional Chinese medicine ferment. The traditional Chinese medicine ferment is prepared from traditional Chinese medicine raw materials and beneficial biological bacteria in a combined mode; the traditional Chinese medicine raw materials comprise fructus crataegi, fructus amomi, tangerine peel, radix glycyrrhizae, fructus tsaoko, cortex cinnamomi, falangal, radix astragali, rhizoma atractylodis macrocephalae, fructus amomi rotundus, pericarpium granati, illicium verum, spica prunellae, rhizoma pinelliae, arisaema cum bile, frankincense, flos caryophylli, fructus foeniculi, radix angelicae dahuricae and moringa oleifera; and the beneficial biological bacteria comprise lactic acid bacteria, bacilli, yeast, clostridium butyricum, lactobacillus paracasei, aspergillosis, trichoderma strains and aspergiilus oryzae. According to the traditional Chinese medicine ferment, biological balance of intestinal flora can be effectively improved, animal immunity is improved, diseases are reduced, and the raditional Chinese medicine ferment can replace antibiotics to be used, is high in feed utilization rate and high in safety, can be suitable for all livestock and poultry and can obviously improve the meat quality of animals.

The invention discloses Candida railenensis strain BY16 with wide antibacterial spectrum and stable effect for preventing and controlling postharvest diseases of fruits and vegetables and a use method thereof. The preservation strain number of the strain in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.14902. The method of using the Candida: activate the strain, ferment and cultivate it with YPD, centrifuge, and use sterile water to make 1×10 ⁸ cells/mL bacterial suspension; put apples, pears, grapes, strawberries, citrus or cherry tomatoes and other fruits and vegetables into the bacterial suspension, soak for 30 seconds, take out, and air-dry; put them in a fresh-keeping box and store at room temperature. This Candida strain can simultaneously control apple blue mold and gray mold, pear blue mold and gray mold, grape gray mold, anthracnose, aspergillosis, black spot, fusarium and powdery mildew, Strawberry gray mold and aspergillosis, citrus penicillium, and cherry tomato gray mold and aspergillosis can reduce losses caused by postharvest diseases and have good application prospects.

Aspergillus fumigatus agar beads and a preparation method thereof are disclosed. Firstly, Aspergillus fumigatus spore suspension is prepared, and is mixed with a potato dextrose agar medium; the mixture is added into a container containing mineral oil and is stirred and centrifuged to form beads; then a sterile phosphate buffer solution is added for washing; centrifugation for oil removing is performed; beads the diameter of which is smaller than 280 [mu]m are screened; and the spore concentration in the agar beads is adjusted to 5*10<6> CFU/mL. The prepared Aspergillus fumigates-containing agar beads are applied for testing animals, compared with control groups, no abnormal clinical symptom is showed in the test, and food intake and weight gain differences are not significant. The agar beads achieve the objective of Aspergillus fumigates inplanting in the lung, are convenient to apply and free of adverse clinical manifestations, can be used for building a poultry Aspergillus fumigatesin-lung inplanting model, and provide a premise for further research of poultry aspergillosis and interactions with the body.

Disclosed is the use of Serum Amyloid P component (SAP) polypeptides for the treatment of Eurotiomycetes fungi infections, in particular for aspergillosis and invasive aspergillosis, alone or in combination with pentraxin-3 (PTX3) polypeptides.

The invention belongs to the technical field of biology, and provides a special primer for detecting SNP sites of invasive aspergillosis risk after allogeneic hematopoietic stem cell transplantation.The special primer comprises a first primer group and a second primer group; the first primer group is a specific PCR primer consisting of three pairs of primer groups; and the second primer group isa single base extension primer consisting of three primers. The invention also provides a preparation of the SNP sites for detecting the invasive aspergillosis risk after allogeneic hematopoietic stemcell transplantation, and an SNP sites information method for detecting the invasive aspergillosis risk after allogeneic hematopoietic stem cell transplantation. By adopting the special primer for detecting the SNP sites of the invasive aspergillosis risk after allogeneic hematopoietic stem cell transplantation provided, the correlation between the gene polymorphism of the three SNP sites relatedto the attack risk of invasive aspergillosis after allogeneic hematopoietic stem cell transplantation and the occurrence risk of invasive aspergillosis after allogeneic hematopoietic stem cell transplantation can be efficiently and simultaneously completed through one-time detection, so that the detection time is greatly shortened, and the cost is saved.

The invention relates to a traditional Chinese medicine composition for treating goose aspergillosis. The traditional Chinese medicine composition is prepared from folium artemisiae argyi, cortex cinnamon, dwarf sheareria herb, rhizoma zingiberis, herba ephedrae, herba asari, semen lepidii and fructus gleditsiae. The traditional Chinese medicine composition can be powder and is prepared through the following preparation steps: (1) weighing each traditional Chinese medicine according to a formula amount; (2) crushing the traditional Chinese medicines and sieving by a sieve with the size of 40 to 100 meshes; (3) mixing for 5 to 15min to obtain traditional Chinese medicine powder; (4) sub-packaging the traditional Chinese medicine powder into bags with the size of 100 to 330g/bag, so as to obtain a finished product. The prescription has the effects of killing insects and sterilizing, clearing away heat and detoxifying, warming lung and resolving fluid, inducing diuresis for removing edema, freeing lung and relieving asthma, freeing orifice and opening and resolving masses and diminishing swelling; clinical examination shows that the curative effect on the goose aspergillosis is remarkable and the healing rate can reach 91.00 percent.