Aspergillus flavus prevention and control composition containing perillaldehyde and calcium ion chelating agent EDTA, and application thereof

A technology of calcium ion chelating agent and perillaldehyde, applied in application, edible seed preservation, biocide, etc., can solve the effect of fungal mycelium growth and sporulation. There are few studies on the effect of EDTA on perilla aldehyde's antibacterial effect Promote effects and other issues, to achieve significant preventive effects, promote autophagy, and inhibit growth

Active Publication Date: 2020-01-24
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a commonly used calcium ion chelating agent, EDTA has little research on its effect on inhibiting fungal mycelial growth and sp

Method used

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  • Aspergillus flavus prevention and control composition containing perillaldehyde and calcium ion chelating agent EDTA, and application thereof
  • Aspergillus flavus prevention and control composition containing perillaldehyde and calcium ion chelating agent EDTA, and application thereof
  • Aspergillus flavus prevention and control composition containing perillaldehyde and calcium ion chelating agent EDTA, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA, comprising A component and B component, the A component is perillaldehyde (Shanghai Bangcheng Chemical Industry, CAS#: 2111-75- 3), the B component is calcium ion chelating agent EDTA; wherein the concentration of the A component is 100µg / L, and the concentration of the B component is 200µg / L.

[0021] The preparation method of the above-mentioned Aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA comprises the following steps: weighing the corresponding weight component raw materials according to the concentration requirements of each component in the composition, and the solid raw material of the A component Dissolve perillaldehyde in 1 times the volume of absolute ethanol first, then use sterile water to dissolve it completely, then mix it with component B calcium ion chelating agent EDTA, and make the composition of perillald...

Embodiment 2

[0023] The composition in Example 1 was selected to detect its inhibitory effect on Aspergillus flavus. On the ultra-clean workbench, first inoculate the Aspergillus flavus isolated from Fujian onto the PDA solid medium. After 48-72 hours of dark cultivation at a temperature of 29°C, inoculate the cultured Aspergillus flavus 5 mm into 5 In different media, at 29°C, after 4-5 days of dark cultivation, the growth rate of pathogenic bacteria was counted by the cross method.

[0024] 5 different media are

[0025] Control group (CK): PDA solid medium;

[0026] Treatment group 1 (PAE): PDA solid medium + 100µg / L PAE;

[0027] Treatment group 2 (EDTA): PDA solid medium + 200µg / L EDTA;

[0028] Treatment group 3 (PAE + EDTA): PDA solid medium + 100µg / L PAE + 200µg / L EDTA

[0029] Treatment group 4 (PAE + Ca 2+ ): PDA solid medium + 100µg / L PAE + 1.11g / LCaCl 2 .

[0030] The statistics of growth rate found that the medium containing 100µg / L PAE, 200µg / L EDTA, and 100µg / L PAE+20...

Embodiment 3

[0032] The composition in Example 1 was selected to detect its inhibitory effect on the germination of Aspergillus flavus spores and the concentration of intracellular calcium ions. On the ultra-clean workbench, first inoculate the isolated Aspergillus flavus from Fujian on the PDA solid medium, and culture it in the dark at 29°C for 5-7 days, then collect the spores to prepare 1×10 6 Aspergillus flavus spore suspension, take 2 μL spore suspension and inoculate to final concentrations of 100µg / L PAE, 200µg / L EDTA, 100µg / L PAE+200µg / L EDTA and 100µg / L PAE PAE+1.11g / LCaCl 2 The germination rate was counted and photographed after culturing in the dark at 29°C for 5-6 hours on a PDA plate, and water treatment was used as a control. According to the statistics of germination rate, 100µg / L PAE, 200µg / L EDTA, 100µg / L PAE+200µg / L EDTA can all inhibit germination, but 100µg / L PAE+1.11g / LCaCl 2 The treatment had no inhibitory effect on spore germination ( figure 2 A). The germinat...

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Abstract

The invention provides an Aspergillus flavus prevention and control composition containing perillaldehyde and a calcium ion chelating agent EDTA, and an application thereof. The composition comprisesa component A and a component B, the component A is perillaldehyde, the component B is the calcium ion chelating agent EDTA, the concentration of perillaldehyde is 100-1000 [mu]g/L, and the concentration of the calcium ion chelating agent EDTA is 200-1000 [mu]g/mL. The composition has a strong inhibiting effect on peanut Aspergillus flavus, and can be used for preparing medicines for preventing and controlling peanut Aspergillus flavus. The perillaldehyde is natural, has no toxic or side effect, does not easily cause pathogenic microorganisms to generate pesticide resistance, and can be used for preventing and controlling peanut Aspergillus flavus; and the composition is safe to human and livestock, and is a low-toxicity effective antibacterial biological pesticide. The perillaldehyde andthe calcium ion chelating agent EDTA have huge potential as the Aspergillus flavus bactericide.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a peanut aflatoxin control composition containing perillaldehyde and a calcium ion chelating agent EDTA and an application thereof. Background technique [0002] Aspergillus flavus is a pathogen of plants, animals and humans, causing losses in the tens of billions of dollars each year. More than 1 / 4 of the crops in the world are polluted by Aspergillus flavus every year. During the storage and transportation of grains after harvest, Aspergillus flavus strains can infect grains and produce a large amount of carcinogenic and acute toxic compounds - aflatoxins. Under normal circumstances, peanuts and their products in oil crops have the most chance of being contaminated. For many years, the prevention and control of Aspergillus flavus has been mainly based on chemical control. However, most of the chemical pesticides are non-naturally occurring substances, and ...

Claims

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Application Information

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IPC IPC(8): A01N35/02A01N37/44A01P3/00A23B9/26
CPCA01N35/02A01N37/44A23B9/26
Inventor 刘裴清陈庆河李本金王荣波翁启勇
Owner INST OF PLANT PROTECTION FAAS
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