Aspergillus flavus prevention and control composition containing perillaldehyde and calcium ion chelating agent EDTA, and application thereof
A technology of calcium ion chelating agent and perillaldehyde, applied in application, edible seed preservation, biocide, etc., can solve the effect of fungal mycelium growth and sporulation. There are few studies on the effect of EDTA on perilla aldehyde's antibacterial effect Promote effects and other issues, to achieve significant preventive effects, promote autophagy, and inhibit growth
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Embodiment 1
[0020] A flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA, comprising A component and B component, the A component is perillaldehyde (Shanghai Bangcheng Chemical Industry, CAS#: 2111-75- 3), the B component is calcium ion chelating agent EDTA; wherein the concentration of the A component is 100µg / L, and the concentration of the B component is 200µg / L.
[0021] The preparation method of the above-mentioned Aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA comprises the following steps: weighing the corresponding weight component raw materials according to the concentration requirements of each component in the composition, and the solid raw material of the A component Dissolve perillaldehyde in 1 times the volume of absolute ethanol first, then use sterile water to dissolve it completely, then mix it with component B calcium ion chelating agent EDTA, and make the composition of perillald...
Embodiment 2
[0023] The composition in Example 1 was selected to detect its inhibitory effect on Aspergillus flavus. On the ultra-clean workbench, first inoculate the Aspergillus flavus isolated from Fujian onto the PDA solid medium. After 48-72 hours of dark cultivation at a temperature of 29°C, inoculate the cultured Aspergillus flavus 5 mm into 5 In different media, at 29°C, after 4-5 days of dark cultivation, the growth rate of pathogenic bacteria was counted by the cross method.
[0024] 5 different media are
[0025] Control group (CK): PDA solid medium;
[0026] Treatment group 1 (PAE): PDA solid medium + 100µg / L PAE;
[0027] Treatment group 2 (EDTA): PDA solid medium + 200µg / L EDTA;
[0028] Treatment group 3 (PAE + EDTA): PDA solid medium + 100µg / L PAE + 200µg / L EDTA
[0029] Treatment group 4 (PAE + Ca 2+ ): PDA solid medium + 100µg / L PAE + 1.11g / LCaCl 2 .
[0030] The statistics of growth rate found that the medium containing 100µg / L PAE, 200µg / L EDTA, and 100µg / L PAE+20...
Embodiment 3
[0032] The composition in Example 1 was selected to detect its inhibitory effect on the germination of Aspergillus flavus spores and the concentration of intracellular calcium ions. On the ultra-clean workbench, first inoculate the isolated Aspergillus flavus from Fujian on the PDA solid medium, and culture it in the dark at 29°C for 5-7 days, then collect the spores to prepare 1×10 6 Aspergillus flavus spore suspension, take 2 μL spore suspension and inoculate to final concentrations of 100µg / L PAE, 200µg / L EDTA, 100µg / L PAE+200µg / L EDTA and 100µg / L PAE PAE+1.11g / LCaCl 2 The germination rate was counted and photographed after culturing in the dark at 29°C for 5-6 hours on a PDA plate, and water treatment was used as a control. According to the statistics of germination rate, 100µg / L PAE, 200µg / L EDTA, 100µg / L PAE+200µg / L EDTA can all inhibit germination, but 100µg / L PAE+1.11g / LCaCl 2 The treatment had no inhibitory effect on spore germination ( figure 2 A). The germinat...
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