6 results about "Aspergillus nidulans" patented technology

The invention belongs to the technical field of gene engineering and microbial fermentation, and particularly discloses a cytochrome P450 reductase coding gene CPR2 from aspergillus nidulans and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. According to the invention, the CPR2 gene (OECPR2) is identified as an optimal electron transfer regulatory element by performing function screening on eight CPR genes annotated in a genome, and the CPR2 gene is over-expressed in an echinocandin B production strain aspergillus nidulans, so that the intracellular electron transfer efficiency is remarkably enhanced, the enzyme catalytic activity of cytochrome P450 is driven, and the cytochrome P450 can be used for preparing an echinocandin B cell. And finally, the yield of echinocandin B is efficiently increased. Experiments show that the engineering strain for over-expression of CPR2 can enable the yield of echinocandin B to reach 1869.97 + / -96.98 mg / L in shake flask fermentation, which is 24.37% higher than that of the original strain. The invention provides a key gene element and an engineering strain for industrial efficient production of echinocandin B, and has important economic value.

The invention discloses an engineering strain with high yield of echinocandin B as well as a construction method and application of the engineering strain, and belongs to the technical field of gene engineering. According to the invention, wild aspergillus nidulans BGCm5 is modified by a CRISPR / nCas9-CBE (clustered regularly interspaced short palindromic repeats / nCRas9-CBE (clustered regularly interspaced short palindromic repeats / nCRas9-CBE) technology, so that nutritional deficient strains of NHEJ (NkuA mutation), pyridoxine (PyroA mutation) and riboflavin (RiboB mutation) are obtained. And then, introducing an orthogonal Lox site by utilizing CRISPR / Cas9, and constructing a chassis cell. Through a Cre / LoxP and Cre / Lox2272 recombination system, a combined overexpression cassette of aniF, aniF 2, aniG, aniH, aniI, aniK and odeA genes is integrated into a genome, a series of engineering strains are obtained, and the production efficiency is remarkably improved.

This invention discloses a method for heterologous production of 7-aminodeacetoxycephalosporanic acid using *Aspergillus nidulans*. This invention targets a *Aspergillus nidulans* strain that heterologously produces 7-aminodeacetoxycephalosporanic acid. Genes derived from the biosynthetic gene clusters pcbAB / C, isopentine N-acetyltransferase gene, and cefE gene, or their mutants, are assembled into a *Aspergillus nidulans* expression vector. Co-expression is performed in a *Aspergillus nidulans* basal cell strain to obtain a recombinant strain, successfully achieving heterologous expression of 7-aminodeacetoxycephalosporanic acid in filamentous fungi. Experiments show that the yield can reach 0.13 g / L at the shake-flask level. The strain constructed in this invention provides a good heterologous expression system for studying the synthesis and regulatory mechanism of 7-aminodeacetoxycephalosporanic acid.

The application relates to the technical field of sludge dewatering treatment, and more particularly to a flocculating agent and a sludge dewatering and solidification treatment process.A flocculating agent comprises the following raw materials in parts by weight: 16-20 parts of chitin, 4-6 parts of an Aspergillus fermentation liquor, 2-3 parts of PLA-g-CS, 10-12 parts of an epoxy group quaternary ammonium salt and 8-12 parts of a calcium salt, the Aspergillus fermentation liquor contains Aspergillus niger and Aspergillus nidulans, and the PLA-g-CS is prepared from carboxyl-terminated polylactic acid grafted chitosan.The flocculating agent PLA-g-CS of the application serves as a growth carrier for Aspergillus niger and Aspergillus nidulans in the Aspergillus fermentation liquor, and chitin and quaternary ammonium salt groups are introduced at chitosan on the PLA-g-CS, so that the flocculating agent obtains stronger flocculation effect, and the flocculating agent has better biodegradability, reducing the pollution of the flocculating agent to the environment.

The invention discloses a fatty acid beta-oxidation pathway coding gene derived from aspergillus nidulans and application of the fatty acid beta-oxidation pathway coding gene. The coding gene is selected from the following groups: (a) an Aspergillus 0G048390 gene with a nucleotide sequence as shown in SEQ ID NO: 1; (b) an Aspergillus 0G044330 gene of which the nucleotide sequence is as shown in SEQ ID NO: 3; and / or (c) an Aspergillus 0G004350 gene of which the nucleotide sequence is as shown in SEQ ID NO: 5. The engineering strain constructed by the invention has the advantages that the yield of echinocandin B is obviously increased; in shake flask fermentation, the yield of the overexpressed strain is improved by 33.55%, 25.14% and 25.62% compared with that of the original strain, important technical support is provided for industrial efficient production of echinocandin B, and the Echinocandin B strain has remarkable economic value and application prospect.

The invention relates to an aspergillus nidulans strain with high yield of theabrownin and application thereof. The aspergillus nidulans strain is preserved in China General Microbiological Culture Collection Center on December 1, 2025, the preservation name of the aspergillus nidulans strain is Aspergillus nidulans A-17, and the preservation number of the aspergillus nidulans strain is CGMCC (China General Microbiological Culture Collection Center) No. 42334. The aspergillus nidulans provided by the invention has efficient theabrownin production capacity, does not produce mycotoxin, and can be applied to theabrownin industrial production; meanwhile, the strain can also be inoculated with tea leaves and a tea extracting solution for fermentation, and is used for industrially producing high-theabrownin dark tea and high-theabrownin instant tea. In addition, the strain can significantly reduce the delicate flavor and astringent taste of tea leaves, endows the tea leaves with stale flavor and mellow taste, and can be used for producing high-quality dark tea. The aspergillus nidulans provided by the invention has strong capability of producing theabrownin, has no safety risk, provides high-quality microbial germplasm resources for preparing theabrownin, high-theabrownin tea products and high-quality dark tea, and has a good industrial application prospect.