19 results about "Aspergillus nidulans" patented technology

The invention discloses an emodin anthraquinone high-yield engineering strain. According to the invention, mdpG and mdpF genes of aspergillus nidulans are exogenous introduced into a chassis strain aspergillus nidulans LO8030, so that the genetically engineered bacterium for producing emodin is obtained. Furthermore, the g1234 gene is knocked out from the genetically engineered bacterium for producing the emodin, so that the yield of the emodin can be obviously improved. The decarboxylase gene is further introduced in an exogenous manner, so that the yield of the emodin can be further remarkably improved. And on the basis, exogenous introduction of mdpK, mdpC and mdpB genes is carried out, such that the chrysophanol-producing genetically engineered bacterium is obtained. Or the rugG gene from talaromyces sp.YE3016 is introduced in an exogenous manner, so that the genetically engineered bacterium for producing the muscardine hydrochloride is obtained.

The invention discloses a glucose-6-phosphate dehydrogenase mutant, a biological material, a catalyst and application, and belongs to the field of protein engineering, the mutant is based on a wild glucose-6-phosphate dehydrogenase amino acid sequence derived from Aspergillus nidulans FGSC A4, and has one or more amino acid residue mutations in the 111st site, the 127th site, the 214th site and the 217th site. The NADPH regeneration efficiency of the mutant disclosed by the invention is remarkably improved, and the catalytic efficiency of the preferable mutant K111L / T217L is 2.06 times that of wild glucose-6-phosphate dehydrogenase; the obtained mutant has wider reaction conditions, and has good NADPH regeneration efficiency under the conditions that the temperature is 20-50 DEG C and the pH is 6-10.

The present invention addresses the problem of providing an enzyme agent containing a novel aminopeptidase that exhibits substrate specificity different from that of conventional aminopeptidase. The present invention relates to an enzyme agent comprising, as an active ingredient, aminopeptidase comprising the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 8 or an amino acid sequence equivalent thereto. Furthermore, the present invention relates to an enzyme agent which contains, as an active ingredient, an aminopeptidase that is derived from Aspergillus nidulans or Aspergillus oryzae and has the following physicochemical properties. (1) the optimum temperature is about 50 DEG C; and (2) the optimum pH is about 7-8. And (3) primer specificity: the primer has the highest specificity on L-alanine residues or L-lysine residues.

The invention discloses chitinase activity and application of a trehalose-6-phosphate synthase homologous protein. The trehalose-6-phosphate synthase is selected from any one of the following proteins: A1) a protein as shown in any one of SEQ ID NO.1-8; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid as shown in any one of SEQ ID NO.1-8 and has chitin synthase activity; a3) a protein derived from Trichoderma, yeast, Aspergillus nidulans, Aspergillus fumigatus, Candida albicans, Magnaporthe oryzae, Alternaria alternata and Escherichia coli, having 98% or more of identity with the protein of A1) and having chitin synthase activity; and A4) a fusion protein obtained by connecting a label to the N end or C end of the sequence A1) or A2) or A3). The Tpss disclosed by the invention has chitin synthase activity, can be used for synthesizing chitin from UDP-GlcNAc, and has a very good application prospect.

The invention belongs to the technical field of gene engineering and microbial fermentation, and particularly discloses a cytochrome P450 reductase coding gene CPR2 from aspergillus nidulans and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. According to the invention, the CPR2 gene (OECPR2) is identified as an optimal electron transfer regulatory element by performing function screening on eight CPR genes annotated in a genome, and the CPR2 gene is over-expressed in an echinocandin B production strain aspergillus nidulans, so that the intracellular electron transfer efficiency is remarkably enhanced, the enzyme catalytic activity of cytochrome P450 is driven, and the cytochrome P450 can be used for preparing an echinocandin B cell. And finally, the yield of echinocandin B is efficiently increased. Experiments show that the engineering strain for over-expression of CPR2 can enable the yield of echinocandin B to reach 1869.97 + / -96.98 mg / L in shake flask fermentation, which is 24.37% higher than that of the original strain. The invention provides a key gene element and an engineering strain for industrial efficient production of echinocandin B, and has important economic value.

The invention discloses an engineering strain with high yield of echinocandin B as well as a construction method and application of the engineering strain, and belongs to the technical field of gene engineering. According to the invention, wild aspergillus nidulans BGCm5 is modified by a CRISPR / nCas9-CBE (clustered regularly interspaced short palindromic repeats / nCRas9-CBE (clustered regularly interspaced short palindromic repeats / nCRas9-CBE) technology, so that nutritional deficient strains of NHEJ (NkuA mutation), pyridoxine (PyroA mutation) and riboflavin (RiboB mutation) are obtained. And then, introducing an orthogonal Lox site by utilizing CRISPR / Cas9, and constructing a chassis cell. Through a Cre / LoxP and Cre / Lox2272 recombination system, a combined overexpression cassette of aniF, aniF 2, aniG, aniH, aniI, aniK and odeA genes is integrated into a genome, a series of engineering strains are obtained, and the production efficiency is remarkably improved.

This invention discloses a method for heterologous production of 7-aminodeacetoxycephalosporanic acid using *Aspergillus nidulans*. This invention targets a *Aspergillus nidulans* strain that heterologously produces 7-aminodeacetoxycephalosporanic acid. Genes derived from the biosynthetic gene clusters pcbAB / C, isopentine N-acetyltransferase gene, and cefE gene, or their mutants, are assembled into a *Aspergillus nidulans* expression vector. Co-expression is performed in a *Aspergillus nidulans* basal cell strain to obtain a recombinant strain, successfully achieving heterologous expression of 7-aminodeacetoxycephalosporanic acid in filamentous fungi. Experiments show that the yield can reach 0.13 g / L at the shake-flask level. The strain constructed in this invention provides a good heterologous expression system for studying the synthesis and regulatory mechanism of 7-aminodeacetoxycephalosporanic acid.

The invention relates to the field of microbial synthesis. The invention provides an Aspergillus nidulans engineering strain AnDAOC-1. The Aspergillus nidulans engineering strain AnDAOC-1 is obtained by transferring a plasmid pDAOC-1 containing a pcbAB gene, a plasmid pDAOC-2 containing a cefD1 / cefD2 / cefM / cefT gene and a plasmid pDAOC-3 containing a pcbC / SccefE / cefP gene into an Aspergillus nidulans A1145 strain together, and the Aspergillus nidulans engineering strain AnDAOC-1 is obtained. According to the strain AnDAOC-1 provided by the invention, a biosynthesis path of the deacetoxycephalosporin C is successfully opened in aspergillus nidulans, heterologous expression of the deacetoxycephalosporin C is realized, and the yield of the deacetoxycephalosporin C reaches 0.593 g / L at a shake flask level. The synthesis of the deacetoxycephalosporin C in the aspergillus nidulans is realized for the first time. The strain constructed by the invention provides a good expression system for heterologous synthesis of the deacetoxycephalosporin C and exploration of synthesis and regulation mechanisms of the deacetoxycephalosporin C in cephalosporium acremonium.

The present invention discloses a cytokinin compound with broad biological effects, its preparation method, and application. The cytokinin compound is derived from Fusarium thaliana and is synthesized by the fexA and fexC genes of the cytokinin gene cluster. The compound can be produced by heterologously expressing the fexA and fexC genes in Aspergillus nidulans. Biological activity assays revealed that the compound exhibited superior activity compared to the previously reported cytokinin iP in inducing tobacco callus growth, promoting betalain production in amaranth, inhibiting Arabidopsis taproot growth, and delaying wheat leaf senescence. This provides more efficient and safe cytokinin drug candidates for future agricultural production.

The application relates to the technical field of sludge dewatering treatment, and more particularly to a flocculating agent and a sludge dewatering and solidification treatment process.A flocculating agent comprises the following raw materials in parts by weight: 16-20 parts of chitin, 4-6 parts of an Aspergillus fermentation liquor, 2-3 parts of PLA-g-CS, 10-12 parts of an epoxy group quaternary ammonium salt and 8-12 parts of a calcium salt, the Aspergillus fermentation liquor contains Aspergillus niger and Aspergillus nidulans, and the PLA-g-CS is prepared from carboxyl-terminated polylactic acid grafted chitosan.The flocculating agent PLA-g-CS of the application serves as a growth carrier for Aspergillus niger and Aspergillus nidulans in the Aspergillus fermentation liquor, and chitin and quaternary ammonium salt groups are introduced at chitosan on the PLA-g-CS, so that the flocculating agent obtains stronger flocculation effect, and the flocculating agent has better biodegradability, reducing the pollution of the flocculating agent to the environment.

The invention provides a high-yield lichen acid engineering bacterium YAFWY001 and a culture method thereof, and relates to the technical field of microbial biosynthesis. The invention provides an engineering bacterium capable of producing serotonic acid, the engineering bacterium is prepared by transferring a yARGAorsA expression element into a pyrG gene-deleted aspergillus nidulans strain, the yARGAorsA expression element is formed by linking genes Ya5, pyrG, Rib, gbdA (p), OrsA and Ya3 in sequence from the 5end, and the engineering bacterium capable of producing serotonic acid provided by the invention is characterized in that magnesium acetate (YGM) is added into a YG liquid culture medium, so that the engineering bacterium capable of producing serotonic acid is obtained. The content of the lichen acid in the fermentation liquor of the lichen acid producing engineering strain can be obviously increased, and the method is a new way for obtaining the lichen acid at low cost.

The invention discloses a fatty acid beta-oxidation pathway coding gene derived from aspergillus nidulans and application of the fatty acid beta-oxidation pathway coding gene. The coding gene is selected from the following groups: (a) an Aspergillus 0G048390 gene with a nucleotide sequence as shown in SEQ ID NO: 1; (b) an Aspergillus 0G044330 gene of which the nucleotide sequence is as shown in SEQ ID NO: 3; and / or (c) an Aspergillus 0G004350 gene of which the nucleotide sequence is as shown in SEQ ID NO: 5. The engineering strain constructed by the invention has the advantages that the yield of echinocandin B is obviously increased; in shake flask fermentation, the yield of the overexpressed strain is improved by 33.55%, 25.14% and 25.62% compared with that of the original strain, important technical support is provided for industrial efficient production of echinocandin B, and the Echinocandin B strain has remarkable economic value and application prospect.

The invention discloses an application of a mutant topoisomerase II coding gene in enhancing the acid resistance of fungi, and belongs to the field of molecular biology. According to the invention, point mutation is carried out on the topoisomerase II coding gene of the model fungus Aspergillus nidulans, and the topoisomerase II structure is partially damaged, so that the acid resistance of the Aspergillus nidulans is enhanced, and the addition amount of a neutralizing agent in the production of L-malic acid is further reduced. The method is a brand new method capable of enhancing the acid resistance of the fungi, and can effectively solve the problems that the cost is increased and waste is generated to pollute the environment due to the fact that the existing fungi cannot tolerate a low-pH environment and a neutralizer needs to be continuously added in the organic acid production process.

The present invention discloses a glucose-6-phosphate dehydrogenase mutant, biomaterial, catalyst and application, belonging to the field of protein engineering. Aspergillus nidulans FGSC A4 The amino acid sequence of the wild-type glucose-6-phosphate dehydrogenase derived from the present invention has one or more mutations in amino acid residues at positions 111, 127, 214, and 217. The NADPH regeneration efficiency of the mutant disclosed in the present invention is significantly improved. The catalytic efficiency of the preferred mutant K111L / T217L is 2.06 times that of the wild-type glucose-6-phosphate dehydrogenase. The reaction conditions of the obtained mutant are wider, and the NADPH regeneration efficiency is good under the conditions of temperature of 20-50°C and pH of 6-10.

The invention relates to an aspergillus nidulans strain with high yield of theabrownin and application thereof. The aspergillus nidulans strain is preserved in China General Microbiological Culture Collection Center on December 1, 2025, the preservation name of the aspergillus nidulans strain is Aspergillus nidulans A-17, and the preservation number of the aspergillus nidulans strain is CGMCC (China General Microbiological Culture Collection Center) No. 42334. The aspergillus nidulans provided by the invention has efficient theabrownin production capacity, does not produce mycotoxin, and can be applied to theabrownin industrial production; meanwhile, the strain can also be inoculated with tea leaves and a tea extracting solution for fermentation, and is used for industrially producing high-theabrownin dark tea and high-theabrownin instant tea. In addition, the strain can significantly reduce the delicate flavor and astringent taste of tea leaves, endows the tea leaves with stale flavor and mellow taste, and can be used for producing high-quality dark tea. The aspergillus nidulans provided by the invention has strong capability of producing theabrownin, has no safety risk, provides high-quality microbial germplasm resources for preparing theabrownin, high-theabrownin tea products and high-quality dark tea, and has a good industrial application prospect.

The invention discloses a bifluorescence detection method for detecting gene expression in filamentous fungi and an exogenous gene expression method. The invention provides a double-fluorescence detection system and a double-fluorescence detection method capable of efficiently and rapidly detecting the expression efficiency of different promoters and insertion site genes of filamentous fungi, the influence caused by the growth difference of strains can be eliminated, the quantitative detection of the gene expression condition is realized, and the advantages of high efficiency and accuracy are achieved. Six efficient insertion sites are screened out, more possibilities are provided for successful heterologous expression of complex high-value natural products in aspergillus nidulans, and high application value is achieved.

The present invention provides a high-mossinoic acid-producing engineered bacterium, YAFWY001, and a cultivation method thereof, relating to the technical field of microbial biosynthesis. The present invention provides a mossinoic acid-producing engineered bacterium, prepared by transferring the yARGAorsA expression element into an Aspergillus nidulans strain lacking the pyrG gene. The yARGAorsA expression element is composed, from the 5' end, of the genes Ya5, pyrG, Rib, gbdA(p), OrsA, and Ya3, linked in sequence. The present invention provides a mossinoic acid-producing engineered bacterium. Adding magnesium acetate (YGM) to the YG liquid culture medium significantly increases the mossinoic acid content in the fermentation broth of the mossinoic acid-producing engineered strain. This invention provides a new, low-cost approach to obtaining mossinoic acid.

The invention relates to the field of microbial synthesis, in particular to application of a gene in heterologous synthesis of deacetylcephalosporin C, a plasmid and a strain. The invention provides an aspergillus nidulans engineering strain AnDCPC-1. The aspergillus nidulans engineering strain AnDCPC-1 is obtained by transferring a plasmid pDCPC-1 containing a pcbAB gene, a plasmid pDCPC-2 containing a cefD1 / cefD2 / cefM / cefT gene, and a plasmid pDCPC-3 containing a pcbC / cefEF / cefP gene into an aspergillus nidulans A1145 strain together. According to the strain AnDCPC-1 disclosed by the invention, a biosynthesis path of the deacetylated cephalosporin C is successfully opened in aspergillus nidulans, heterologous expression of the deacetylated cephalosporin C is realized, and the yield reaches 0.595 g / L at a shake flask level. The synthesis of the deacetylated cephalosporin C in the aspergillus nidulans is realized for the first time. The strain constructed by the invention provides a good expression system for heterologous synthesis of the deacetylated cephalosporin C and exploration of synthesis and high-yield mechanisms of the deacetylated cephalosporin C in cephalosporium acremonium.

The present invention belongs to the fields of genetic engineering and biocatalysis technology, and specifically relates to an α-L-rhamnosidase mutant and its application. The α-L-rhamnosidase mutant is obtained by mutating glycine at the 227th position of the α-L-rhamnosidase with the amino acid sequence shown in SEQ ID NO.2 into glutamic acid, and the amino acid sequence of the α-L-rhamnosidase mutant is shown in SEQ ID NO.1. The α-L-rhamnosidase gene is derived from Aspergillus nidulans. By using the random mutation technology, an α-L-rhamnosidase mutant expressed in Escherichia coli is successfully screened out, and its enzyme activity is increased by 1.52 times. Using naringin as a substrate, the catalytic reaction is carried out under the conditions of pH = 6.0-7.0 and temperature of 37-38 °C, and the conversion rate of prunin reaches 95%.