Composite microbial inoculum for composting organic materials and preparation method thereof

A technology of composite microbial inoculants and organic materials, which is applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc. Process and steps, easy industrial production, the effect of reducing production costs

Pending Publication Date: 2017-06-16
杨小波
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many similar compost starters on the market, but we learned from the Microbial Fertilizer Registration Center of the Ministry of Agriculture that most of these products are only a simple compound of two or three microbial strains, which are too simple and have poor adaptability. The agent cannot decompose straw materials, and the materials suitable for straw are not suitable for sludge, etc. The effect of using these strains in compost with complex components is not very satisfactory, and the added microbial strains have no eff

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite microbial inoculum for composting organic materials and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example

[0042] 1) Activate the strains stored at 4°C on a slope: Pichia pastoris, Lactobacillus acidophilus, Bacillus coagulans, Enterococcus faecium, Bacillus natto, and Bacillus subtilis were inoculated on the LB solid slope; Aspergillus nidulans, white rot Fungi, Aspergillus usa, Trichoderma harcii, Trichoderma reesei and Aspergillus niger were inoculated on the solid slope of PDA. All bacteria were activated at 35°C.

[0043] 2) Preparation of primary seed liquid: Inoculate the activated test tube slant bacteria into corresponding liquid medium (Pichia pastoris, Lactobacillus acidophilus, Bacillus coagulans, Enterococcus faecium, Bacillus natto, subtilis Bacillus was inoculated in LB liquid shake flasks; Aspergillus nidulans, white rot fungi, Aspergillus usa, Trichoderma harcii, Trichoderma reesei, and Aspergillus niger were inoculated in PDA liquid shake flasks), all strains were inoculated at 35°C and 200rpm Cultivate for 4 days to obtain liquid primary seeds.

[0044]3) Prepa...

Embodiment 2

[0049] Application Effect Experiment of Composting Bacteria Agent

[0050] Raw material ratio of compost:

[0051] materials domestic sludge corn husk sugar residue Furfural residue Proportion 40% 20% 20% 20%

[0052] The moisture content of the fermentation material is controlled at 60%, and the raw materials for the production of 10 tons of finished organic fertilizers are divided into two equal parts: Group 1: add strains developed by our company; Group 2: control without strains

[0053] Adding method of bacteria agent: Activate the bacteria two days before composting, mix 1kg of bacteria with 5kg of rice bran, dissolve 300g of brown sugar in 1kg of water, mix the sugar water and bacteria of rice bran evenly, and then add appropriate amount of water to adjust the material Humidity at 60%, activation at 30°C for 2 days

[0054] Mix the material with the activated bacterial agent evenly (1 ton of material is mixed with 5kg of the activated bac...

Embodiment 3

[0062] Fertilizer effect experiment on corn:

[0063] The organic fertilizers produced by adding bacteria and not adding bacteria were used as the base fertilizer respectively, and the method of strip application was adopted, and the dosage was 2 tons per mu. The rest of the fertilization amount of corn is carried out in the following way:

[0064] a: Base fertilizer: N3.45g, P2O52.88g, zinc sulfate 0.72g (converted to urea 7.5g, common calcium 18g)

[0065] b: Topdressing: deep application of N8.06g and K2O3.36g (converted to urea, 17.5g, potassium chloride 5.6g) in combination with large potting soil during the big trumpet mouth period

[0066] Add bacteria compost group Compost without added bacteria No fertilization blank control Yield kg / mu 800 600 400

[0067] From the experimental results, it can be seen that the organic fertilizer with added bacteria has obvious fertilizer effect, which is 50% higher than that of the blank control group an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a composite microbial inoculum for composting organic materials and a preparation method thereof. The microbial inoculum mainly comprises pichia pastoris, lactobacillus acidophilus, bacillus coagulans, enterococcus faecalis, bacillus natto, bacillus subtilis, aspergillus nidulans, white-rot fungi, aspergillus usamii, trichoderma harzianum, trichoderma reesei and aspergillus niger. The preparation method comprises the following steps: first classifying the microorganisms into bacteria, yeasts and filamentous fungi, then performing mixed culture on the same class of microorganisms till quality requirements are met, and then compounding according to the following proportions: 20-70 parts of bacteria, 30-60 parts of yeasts and 50-80 parts of filamentous fungi. The compositing microbial inoculum prepared by the preparation method has the advantages of fast fermentation start during composting fermentation, high temperature in the composting process, a long high-temperature period and fast organic material decomposition. An organic fertilizer prepared by using the microbial inoculum is good in quality; compost has a fertility-retaining function, has high effective utilization rate of a large number of elements such as nitrogen, phosphorus and potassium, and has the effects of dissolving phosphorus, dissolving potassium and fixing nitrogen; compared with a control group which is not inoculated with the microbial inoculum, a product provided by the invention has the advantages as follows: the content of water-soluble organic carbon is increased by about 2 times, the total humic acid content is increased by about 1 times or above, and the content of biochemical fulvic acid is increased by about 2 times.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, in particular to the field of environmental microorganism technology and solid waste treatment technology. Background technique [0002] In recent years, with the vigorous development of organic agriculture, there has been an upsurge of organic planting in China, which has promoted the development of the organic fertilizer industry. According to statistics, the country produces 200-300 million tons of organic fertilizers every year. Such a huge organic fertilizer production also requires a lot of organic fertilizer starter. In the process of organic fertilizer production, various microbial strains that can quickly decompose starch, protein, fiber, etc. are required, and these strains need to cooperate with each other to degrade various organic macromolecules. At present, there are many similar compost starters on the market, but we learned from the Microbial Fertilizer Registration Center of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C05F15/00C05F17/00C12R1/84C12R1/23C12R1/07C12R1/01C12R1/125C12R1/66C12R1/645C12R1/885C12R1/685
CPCC12N1/14C12N1/16C12N1/20C05F5/006C05F11/08C05F7/00C05F11/00C05F17/00Y02W30/40
Inventor 杨小波
Owner 杨小波
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap