Method for producing chitosan oligosaccharide from home-made enzyme solution

A technology of chitosan oligosaccharides and enzyme solution, which is applied in the field of enzymatic production of chitosan oligosaccharides, which can solve problems such as unstable product quality, high energy consumption, and damage to the surrounding ecological environment, so as to reduce technical risks, mild reaction conditions, and ensure stability sexual effect

Inactive Publication Date: 2016-07-27
ZHUHAI JINLONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many problems: such as long reaction time, high energy consumption, unstable product quality, especially serious emissions, causing huge environmental pollution and serious damage to the surrounding ecological environment. Enzyme preparation solves the above problems, so the enzyme has huge potential. Industrial application value, currently cultivating and optimizing chitin deacetylase strains has become a researc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]100Kg of chitin purchased from a chitin manufacturer is selected, the chitin content is >95%, and the ash content is 90%. Add deionized water to dilute, make a suspension containing 5% chitosan, adjust pH to 5.5, and keep warm at 45°C. Acetic acid was added dropwise to make chitosan completely soluble in water. An aqueous solution of chitosan is formed. Add 10% enzyme solution B to the chitosan solution, continue to stir, keep warm at 45°C, and react for 5 hours to obtain a chitosan oligosaccharide solution, which is separated and purified by an ultrafiltration membrane and RO reverse osmosis membrane to obtain a cut-off group with a molecular weight of 500-3000 point. Concentrate in vacuo, dry, and obtain 36Kg chitosan oligosaccharide powder with a purity >90% and an ash content <1%.

[0048] The enzyme solution A described in this embodiment is the enzyme solution produced by the fungus Aspergillus nidulans with high yield of chitin deacetylation by liquid fermentat...

Embodiment 2

[0050] 100Kg of chitin purchased from a chitin manufacturer is selected, the chitin content is >95%, and the ash content is 90%. Add deionized water to dilute, make a suspension containing 5% chitosan, adjust pH to 5.5, and keep warm at 45°C. Acetic acid was added dropwise to make chitosan completely soluble in water. An aqueous solution of chitosan is formed. Add 20% enzyme solution B to the chitosan solution, continue to stir, keep warm at 45°C, and react for 5 hours to obtain a chitosan oligosaccharide solution, which is separated and purified by an ultrafiltration membrane and RO reverse osmosis membrane to obtain a cut-off group with a molecular weight of 500-3000 point. Concentrate in vacuo, dry, and obtain 36Kg chitosan oligosaccharide powder with a purity >90% and an ash content <1%.

[0051] The enzyme solution A described in this embodiment is the enzyme solution produced by the fungus Aspergillus nidulans with high yield of chitin deacetylation by liquid fermenta...

Embodiment 3

[0053] 100Kg of chitin purchased from a chitin manufacturer is selected, the chitin content is >95%, and the ash content is 90%. Add deionized water to dilute, make a suspension containing 5% chitosan, adjust pH to 5.5, and keep warm at 45°C. Acetic acid was added dropwise to make chitosan completely soluble in water. An aqueous solution of chitosan is formed. Add 15% enzyme solution B to the chitosan solution, continue to stir, keep warm at 45°C, and react for 8 hours to obtain a chitosan oligosaccharide solution, which is separated and purified by an ultrafiltration membrane and RO reverse osmosis membrane to obtain a cut-off group with a molecular weight of 500-3000. point. Concentrate in vacuo, dry, and obtain 51Kg chitosan oligosaccharide powder with a purity >90% and an ash content <1%.

[0054] The enzyme solution A described in this embodiment is the enzyme solution produced by the fungus Aspergillus nidulans with high yield of chitin deacetylation by liquid ferment...

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Abstract

The invention relates to the technical field of enzymatic production of chitosan oligosaccharide and particularly relates to a low-cost method for producing chitosan oligosaccharide from a home-made enzyme solution. The method comprises the following steps: carrying out fermentation culture on an enzyme solution to prepare fungus aspergillus nidulans capable of highly producing deacetylase and obtain an enzyme solution A for preparing chitosan by virtue of deacetylation of chitin; and preparing an enzyme solution B capable of highly producing cellulose and amylase by virtue of liquid fermentation culture, and preparing chitosan oligosaccharide from the enzyme solution B by virtue of enzymolysis of chitosan. By virtue of a two-step enzymolysis process, chitin firstly reacts with the enzyme solution A to generate chitosan, and chitosan reacts with the enzyme solution B to generate chitosan oligosaccharide. The method has the advantages that the requirements of deacetylation and enzymolysis of chitosan are met by virtue of the home-made enzyme solution, so that the risk of enzyme inactivation of purchased commodities caused due to too many links of drying, storing and transportation is avoided; and a non-specific cheap enzyme source is provided, so that the production cost is lowered, the independence ability of an enterprise is improved, and the stable development of the chitosan oligosaccharide industry is guaranteed.

Description

technical field [0001] The invention relates to the technical field of enzymatically producing chitosan oligosaccharides, in particular to provide a low-cost production method for producing chitosan oligosaccharides by self-made enzyme liquid. Background technique [0002] Chitin, also known as chitin (chintin). The chemical name is (1,4)-2-acetylamino-2-deoxy-β-D-glucose. It is the second largest biological material in nature after cellulose. Chitosan is also known as deacetylated chitin. , he is the product of chitin deacetylation, making its most important derivative, chitin deacetylase (chintindeacetzlaseEC. 3.5.1.41. CDA for short) is a kind of catalyzing N-acetyl-D-glucose in chitin Enzyme for the acetylhydrolysis of glycosamines. At present, chitosan is often produced by chemical methods (sodium hydroxide above 40%). There are many problems: such as long reaction time, high energy consumption, unstable product quality, especially serious emissions, causing huge env...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12P19/14C12N9/80C12N9/42C12N9/28C12R1/07C12R1/66
CPCC12P19/26C12N9/2411C12N9/2437C12N9/80C12P19/14C12Y305/01041
Inventor 徐文中莫锦宏
Owner ZHUHAI JINLONG BIOTECH CO LTD
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