Method for producing chitosan oligosaccharide from home-made enzyme solution

Abstract

The invention relates to the technical field of enzymatic production of chitosan oligosaccharide and particularly relates to a low-cost method for producing chitosan oligosaccharide from a home-made enzyme solution. The method comprises the following steps: carrying out fermentation culture on an enzyme solution to prepare fungus aspergillus nidulans capable of highly producing deacetylase and obtain an enzyme solution A for preparing chitosan by virtue of deacetylation of chitin; and preparing an enzyme solution B capable of highly producing cellulose and amylase by virtue of liquid fermentation culture, and preparing chitosan oligosaccharide from the enzyme solution B by virtue of enzymolysis of chitosan. By virtue of a two-step enzymolysis process, chitin firstly reacts with the enzyme solution A to generate chitosan, and chitosan reacts with the enzyme solution B to generate chitosan oligosaccharide. The method has the advantages that the requirements of deacetylation and enzymolysis of chitosan are met by virtue of the home-made enzyme solution, so that the risk of enzyme inactivation of purchased commodities caused due to too many links of drying, storing and transportation is avoided; and a non-specific cheap enzyme source is provided, so that the production cost is lowered, the independence ability of an enterprise is improved, and the stable development of the chitosan oligosaccharide industry is guaranteed.

Description

technical field [0001] The invention relates to the technical field of enzymatically producing chitosan oligosaccharides, in particular to provide a low-cost production method for producing chitosan oligosaccharides by self-made enzyme liquid. Background technique [0002] Chitin, also known as chitin (chintin). The chemical name is (1,4)-2-acetylamino-2-deoxy-β-D-glucose. It is the second largest biological material in nature after cellulose. Chitosan is also known as deacetylated chitin. , he is the product of chitin deacetylation, making its most important derivative, chitin deacetylase (chintindeacetzlaseEC. 3.5.1.41. CDA for short) is a kind of catalyzing N-acetyl-D-glucose in chitin Enzyme for the acetylhydrolysis of glycosamines. At present, chitosan is often produced by chemical methods (sodium hydroxide above 40%). There are many problems: such as long reaction time, high energy consumption, unstable product quality, especially serious emissions, causing huge env...

AI Technical Summary

Problems solved by technology

There are many problems: such as long reaction time, high energy consumption, unstable product quality, especially serious emissions, causing huge environmental pollution and serious damage to the surrounding ecological environment. Enzyme preparation solves the above problems, so the enzyme has huge potential. Industrial application value, currently cultivating and optimizing chitin deacetylase strains has become a research hotspot in recent years

However, in the actual process of using enzyme degradation technology to produce chitosan oligosaccharides, there are still problems such as high enzyme production costs, unstable product quality, and weak process selectivity.

The fundamental reason is that chitosan oligosaccharide manufacturers cannot master the core technology of enzymatic hydrolysis process: self-production and self-use of enzymes