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Compound microbial inoculum used for waste composting, preparation method of compound microbial inoculum, and waste composting method

A technology of compound bacterial agent and waste, applied in biochemical equipment and methods, preparation of organic fertilizers, methods based on microorganisms, etc., can solve the problems of inability to produce and limit the decomposition effect, and achieve less usage and significant lignin and the effect of cellulolytic function

Inactive Publication Date: 2019-01-22
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single microorganism cannot produce all the enzymes that decompose its macromolecular substances, which limits the decomposition effect of a single microbial agent for landscaping waste

Method used

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  • Compound microbial inoculum used for waste composting, preparation method of compound microbial inoculum, and waste composting method
  • Compound microbial inoculum used for waste composting, preparation method of compound microbial inoculum, and waste composting method
  • Compound microbial inoculum used for waste composting, preparation method of compound microbial inoculum, and waste composting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The screening of embodiment 1 composite bacterial agent microorganism

[0059] The composting experiment at this stage is to screen microorganisms in the decomposing stage. The experiment was carried out at the composting plant of Beijing Botanical Garden.

[0060] 1.1 Composting

[0061] The raw materials are mainly branches and leaves of summer common tree species from Beijing Botanical Garden (including but not limited to ash, poplar, willow, pagoda tree, black locust tree). Branches and leaves are crushed into fragments with a particle size of about 20 mm, which are evenly spread on the ground. The compost materials used in the experiment are collected from the fragments, and then piled up into a conical pile of 1 cubic meter (the base area is 3 square meters, and the height is 1 meter).

[0062] Sub-samples were taken from the top, middle, and bottom of the pile and mixed into one composite sample for chemical analysis (TOC, total carbon: 47.8%, TN (total nitrog...

Embodiment 2

[0081] Embodiment 2 The multiplication and cultivation of 3 bacterial strains

[0082] 1) Propagation and cultivation of Bacillus subtilis and Bacillus thuringiensis

[0083] Inoculate the screened bacteria B1 and B2 into beef extract peptone liquid medium (peptone 10g, beef extract 3g, NaCl 5g, water 1L, pH: natural, high temperature sterilization at 121°C for 30min), the inoculation amount is volume ratio 0.1% (that is, 1ml of bacterial culture solution in a 1L Erlenmeyer flask), cultured for 72h (usually 60-80h) at a culture temperature of (28±2)°C (usually 20-35°C) and a rotation speed of 180rpm; The cultured liquid strain is inoculated into a 5L fermenter equipped with beef extract peptone liquid medium with a volume ratio of 5% inoculum, ventilated culture, culture temperature (28 ± 2) ° C (usually 20-35 ° C ), the stirring speed is 45rpm, the ventilation volume is 1m 3 / L, culture time 28h (usually 20-35h), obtain B1 bacterium liquid, B2 bacterium liquid respectively;...

Embodiment 3

[0087] Example 3 3 bacterial strain dosage screening

[0088] 1) Prepare the compound bacterial agent according to the quadratic regression orthogonal design

[0089] As shown in Tables 1 and 2, according to the code value table and the experimental design table of the quadratic regression orthogonal test, determine the proportioning additions of the three bacterial solutions in each experimental group, and the proportioning additions of the bacterial solutions are shown in Table 3.

[0090] According to the design scheme as shown in Table 1, a quadratic orthogonal regression experiment was carried out to determine the ratio and dosage of the three strains of microorganisms in the composite bacterial agent.

[0091] Table 1 The code value and corresponding addition amount of each diluted bacterial solution

[0092]

[0093] Table 2 quadratic regression orthogonal design table

[0094]

[0095] According to table 1, table 2, the volume consumption of 3 kinds of microbia...

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Abstract

The invention discloses a compound microbial inoculum used for waste composting, a preparation method of the compound microbial inoculum, and a waste composting method adopting the compound microbialinoculum. The compound microbial inoculum comprises bacillus subtilis, bacillus thuringiensis and aspergillus nidulans. Microbes of the compound microbial inoculum have the obvious function of decomposing lignin and cellulose, the compound microbial inoculum prepared by compounding the microbes at a certain ratio (the microbes are separated out at the waste composting maturity stage) is used for carrying out landscaping waste composting, thus the contents of lignin and cellulose in the compost product are obvious reduced, and the degradation rates of lignin and cellulose are improved.

Description

technical field [0001] The invention relates to the composting treatment technology of landscaping waste, in particular to a method for preparing a composite bacterial agent and inoculating it in compost to promote rapid degradation of lignin. Background technique [0002] With the rapid development of urban greening across the country, the amount of landscaping waste such as trimmings of trees, shrubs and grasses, litter, weeds and flowers is increasing, and the treatment methods of incineration and landfill are likely to cause environmental pollution and waste of resources. , the efficient degradation and resource utilization of landscaping waste has become a research hotspot. The main component of landscaping waste is lignocellulose, which is the most abundant renewable energy substance in nature. It is mainly composed of lignin, cellulose and hemicellulose. The structure of lignin is complex and irregular. Embedding cellulose molecules in it, forming a natural barrier, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F11/08C12R1/07C12R1/125C12R1/66
CPCC05F11/08C12N1/14C12N1/20C05F17/20Y02W30/40
Inventor 李素艳
Owner BEIJING FORESTRY UNIVERSITY
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