65 results about "Penicillium expansum" patented technology

The invention belongs to the technical field of grain storage, and discloses a bacillus subtilis strain having broad-spectrum antagonism for main grain storage hazard-moulds, a preparation method of an antifungal metabolite of the bacillus subtilis strain, and application of the bacillus subtilis strain and the antifungal metabolite in grain storage. The strain is bacillus subtilis LM3403, and has a preservation number of CGMCC No. 9377. The bacillus subtilis LM3403 has a better inhibiting effect for the main grain storage hazard-moulds such as fusarium graminearum, fusarium moniliforme, aspergillus flavus, aspergillus niger, aspergillus glaucus, aspergillus fumigatus, penicillium oxalicum, penicillium expansum and mucor mucedo, and is wide in antibacterial spectrum, thus having a giant application potential. The antifungal metabolite of the bacillus subtilis LM3403 has an obvious inhibiting effect for the growth of the main grain storage hazard-moulds. The storage test results prove that after the antifungal metabolite of the bacillus subtilis LM3403 is added into stored grains, the growth of the moulds can be effectively inhibited in a grain storage process, so that the grain storage safety is guaranteed.

The invention relates to cresol trimer compounds as well as a preparation method and application thereof. The compounds with novel structure are prepared from Penicillium expansum 091006 separated from a root sample of Excoecaria agallocha which is a mangrove plant. Proved by experiments, the compounds can be used as a cell proliferation inhibitor or antitumor agent.

The invention relates to the field of microorganisms, and particularly relates to a bacillus cereus wettable powder as well as a preparation method and applications of the bacillus cereus wettable powder. The bacillus cereus wettable powder comprises the following components in percentage by weight: 50-70% of bacillus cereus lyophilized powder, 8-12% of auxiliaries, 0.5-1.5% of a stabilizer, 0.05-0.15% of an ultraviolet protective agent, and the balance of carriers. The bacillus cereus wettable powder is prepared by the following method comprising the following steps: evenly mixing and grinding all the components to obtain powder with meshes of less than or equal to 150, wherein the temperature through the preparation process is held at 0-45DEG C. The bacillus cereus wettable powder plays a good role in inhibiting common fungal diseases such as cryponectria parasitica, stachybotrys atra, fusarium solani, penicillium expansum, colletotrichum gloeosporioides and aspergillus niger during storage of fruits such as Chinese chestnuts, and is non-toxic to human and livestock, and low in cost; and the room-temperature Chinese chestnut storage problem can be well solved, and the storage cost can be lowered.

The invention relates to a microbial composite bacterial preparation and application thereof to preparation of a fermented feed, and belongs to the technical field of biology and the technical field of fermentation. The invention provides a microbial composite bacterial preparation which is high in safety and can effectively inhibit filamentous fungi such as penicillium expansum, aspergillus niger, penicillium roqueforti, penicillium digitatum and the like. The microbial composite bacterial preparation consists of bifidobacterium longum with the preservation number of GDMCC No.60926 and lactobacillus plantarum with the preservation number of CGMCC No.5494; the fermented feed prepared by utilizing the microbial composite bacterial preparation is rich in nutrient, not liable to decay and very high in safety; and the concrete embodiment is that: in the fermented feed prepared by utilizing the microbial composite bacterial preparation, content of mould is 0 CFU / g, and after the fermented feed is placed for 15d at a temperature of 30 DEG C, the content of the mould in the fermented feed prepared by utilizing the microbial composite bacterial preparation is still 0 CFU / g.

The invention discloses a biocontrol Bacillus thuringiensis strain and an application thereof. The collection number of Bacillus thuringiensis BCN10 is CGMCC No: 16675. Fermentation liquor of the Bacillus thuringiensis effectively inhibits common loquat surface putrefying fungi (fusarium oxysporum, botryosphaeria parva, trichoderma aureoviride, colletotrichum gloesporioides and penicillium expansum) on a bi-zone flat plate and a loquat fruit. Volatile substances generated by the fermentation liquor are applied to corrosion resistance in loquat storage, natural putrefaction disease time of theloquat fruit can be delayed, and incidence rate is decreased. Volatile antibacterial substances generated by the Bacillus thuringiensis BCN10 can avoid direct contact with the fruit, the fruit is preserved at normal temperature, food storage time is prolonged, and the strain can be used for preparing a biocontrol preservative which is used for inhibiting the putrefying fungi such as the fusarium oxysporum, the botryosphaeria parva, the trichoderma aureoviride, the colletotrichum gloesporioides and the penicillium expansum and has a wide antibacterial spectrum.

The invention belongs to the technical field of biological control, and particularly relates to application of combination of alginate oligosaccharide and pichia guilliermondii in prevention and control of postharvest penicilliosis of pear fruits and storage and preservation of the pear fruits. The application comprises the steps of: activating and culturing pichia guilliermondii, centrifuging to obtain thalli, preparing a yeast suspension and an alginate oligosaccharide solution by using sterile water, and mixing the two solutions to obtain a mixed solution; after punching the surfaces of pear fruits, injecting the mixed solution, and injecting an isopyknic penicillium expansum spore suspension after 2 hours, so as to effectively control penicilliosis; or directly and uniformly spraying the mixed solution on the surfaces of the pear fruits, and naturally air-drying, so that the application in prevention and control of the postharvest penicilliosis of the pear fruits and storage and preservation of the pear fruits can be implemented. According to the invention, postharvest penicilliosis and natural rot of the pear fruits can be effectively controlled, so that the loss caused by postharvest diseases of the pear fruits is reduced; and meanwhile, the application has no adverse effect on main quality indexes of the harvested pear fruits, is convenient to operate, safe and environment-friendly, and has remarkable economic benefits and social benefits.

The present invention relates to an efficiently-expressed modified penicillium expansum lipase gene, and a plasmid construction method and a protein expression method of the gene. According to the present invention, an obtained hygromycin resistance expression cassette is cloned on target plasmid to obtain hygromycin resistance recombinant plasmid; a PCR technology is adopted to respectively amplify penicillium lipase gene (PEL), Aspergillus nidulans strong promoter glyceraldehyde-3-phosphate dehydrogenase promoter (PgpdA) and Aspergillus nidulans tryptophan synthetase terminator (TtrpC) to obtain a PEL gene expression cassette driven by a strong promoter, and finally the PEL gene expression cassette is inserted into the hygromycin resistance recombinant plasmid to obtain a PEL gene overexpression vector with a hygromycin screening marker. In addition, the obtained efficiently-expressed lipase gene production plasmid is transformed to obtain gene engineering penicillium with high lipase expression, wherein the expression is efficient and stable, and a plurality of advantages are provided compared to the conventional lipase production method.

The invention provides application of luteolin in delaying quality deterioration of harvested fruits. Research finds that the luteolin is externally sprayed on the surfaces of cherry fruits, so that the sensory quality and the oxidation resistance can be remarkably improved; defense reaction and anthocyanin synthesis are induced; and thus, senescence is delayed. The luteolin also can inhibit hyphagrowth of botrytis cinerea and penicillium expansum and reduce pathogenicity of botrytis cinerea and penicillium expansum. Research finds that patulin produced by penicillium expansum can cause cancer and teratogenesis, but luteolin can reduce toxin production of penicillium expansum to a certain extent.