Straw starter and use of the straw starter in straw fermentation

A straw starter, straw technology, applied in the application of straw fermentation, in the field of straw starter, can solve the problems affecting the application and promotion of biological treatment methods, low microbial transformation efficiency, long processing time, etc., and achieves good promotion and application prospects, The effect of improved performance and low equipment requirements can be exploited

Inactive Publication Date: 2012-05-16
北海强兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of biological treatment is low and there is no secondary pollution, but the conversion efficiency of microorganisms used in commonly used biological treatment methods is low and the treatment time is long, which affects the application and promotion of biological treatment methods

Method used

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  • Straw starter and use of the straw starter in straw fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Cultivation of Agaric faecalis

[0031] Inoculate the slanted strain in the liquid shake flask for cultivation, then inoculate the seed liquid in the liquid shake flask into the liquid seed tank for cultivation, and finally inoculate the seed liquid in the liquid seed tank into the fermenter for cultivation. Carry out plate-and-frame pressure filtration, collect mycelium by solid-liquid separation, and adsorb the mycelium with an adsorbent to obtain dry fungus powder.

[0032] Slant culture: prepare slant medium according to the formula of PDA medium, add 1.5% agar, pick the preserved slant strain and insert it into fresh slant medium, cultivate at 28°C for 96 hours, and put it in a 4°C refrigerator. save.

[0033] Shake flask culture: the shake flask culture medium of dung ghost umbrella is (100mL fermentation broth): rapeseed cake 4.0g, urea 0.3g, sucrose 0.025g, MgSO 4 ·7H2O 0.03g, CaCl 2 0.03g, KH 2 PO 4 0.2g, FeSO4 7H 2 O 0.5mg, MnSO 4 ·H 2 O...

Embodiment 2

[0035] Example 2 Cultivation of Penicillium

[0036] Inoculate the slanted strain in the liquid shake flask for cultivation, then inoculate the seed liquid in the liquid shake flask into the liquid seed tank for cultivation, and finally inoculate the seed liquid in the liquid seed tank into the fermenter for cultivation. Carry out plate-and-frame pressure filtration, collect mycelium by solid-liquid separation, and adsorb the mycelium with an adsorbent to obtain dry fungus powder.

[0037] Slant culture: prepare slant medium according to the formula of PDA medium, add 1.2% agar, pick the preserved slant bacteria and insert into fresh slant medium, cultivate at 28°C for 72 hours, and put it in a 4°C refrigerator. save.

[0038] Shake flask culture: Penicillium shake flask culture medium is (100mL fermentation broth): sodium nitrate 0.3g, dipotassium hydrogen phosphate 0.1g, magnesium sulfate 0.05g, potassium chloride 0.05g, ferrous sulfate 0.001g, sucrose 3g; The initial pH w...

Embodiment 3

[0040] The cultivation of embodiment 3 Trichoderma

[0041] Inoculate the slanted strain in the liquid shake flask for cultivation, then inoculate the seed liquid in the liquid shake flask into the liquid seed tank for cultivation, and finally inoculate the seed liquid in the liquid seed tank into the fermenter for cultivation. Carry out plate-and-frame pressure filtration, collect mycelium by solid-liquid separation, and adsorb the mycelium with an adsorbent to obtain dry fungus powder.

[0042] Slant culture: prepare slant medium according to the formula of PDA medium, add 1.5% agar, pick the preserved slant strains and insert into fresh slant medium, cultivate at 28°C for 72 hours, and put it in a 4°C refrigerator. save.

[0043] Shake flask culture: Trichoderma shake flask culture medium is (100mL fermentation broth): sodium nitrate 0.3g, dipotassium hydrogen phosphate 0.1g, magnesium sulfate 0.05g, potassium chloride 0.05g, ferrous sulfate 0.001g, sucrose 3g; The initia...

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Abstract

The invention discloses a straw starter. The straw starter comprises Coprinus sterqulinus, penicillium expansum, trichoderma koningii and Coprinopsis atramentaria. The straw starter can be utilized for microbial fermentation of multiple straws of wheat straws and rice straws, is safe and nontoxic, has strong fermentation condition adaptability, allows a wide temperature range, and can be utilizedfor fermentation at an outdoor temperature of 15 to 35 DEG C. Based on different temperatures, fermentation time is in a range of 20 to 80 days. Straws fermented by the straw starter can be utilized as matrixes of biogas, forage and organic fertilizer.

Description

technical field [0001] The invention relates to a mixture for biotransformation of straw by utilizing microorganisms, in particular to a straw starter comprising a plurality of microorganisms, and its application in straw fermentation. Background technique [0002] The annual output of crop straw in China is about 700 million tons. Due to the transfer of rural labor and the change of farming methods, most of the straw is burned or discarded on the spot. However, the environmental pollution and resource waste caused by in-situ burning or discarding of straw have become the focus of attention of relevant management departments and scientific researchers. More than 60% of straw is cellulose and hemicellulose, which is a very good biomass resource. After biotransformation, it can be widely used in feed, organic fertilizer, biogas, etc. However, the natural structure of straw is very complex, and the biotransformation efficiency is low. It is difficult and costly to directly use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12P5/02A23K1/14C05F11/08C12R1/645C12R1/80C12R1/885
CPCY02P60/87
Inventor 李加友叶小梅阮善明
Owner 北海强兴生物科技有限公司
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