181 results about "Trichoderma koningii" patented technology

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention relates to a method for producing a microbial feed additive by using potato vinasse. The method comprises the following steps of: preparing a vinasse culture medium; mixing bacillus subtilis, trichoderma koningii and bacterial suspension liquid of trichoderma viride spores or mouldy bran, and adding into the vinasse culture medium for culturing and fermenting; and inoculating candida tropicalis or candida utilis seed liquid in the well-fermented culture medium for continuously culturing and fermenting; and finally drying to obtain the microbial feed additive. The invention has the advantages of low energy consumption, low cost, short production period, high production efficiency and recycled resources, reduces the emission of pollutants and is beneficial to building an environment-friendly and resource-saving society; and the produced additive has wide application range, can be added into animal feeds and is a high-quality and highly-efficient microbial fermentation product.

The invention relates to the technical field of soil conditioners and particularly relates to a soil heavy metal biological fixing agent and an application method thereof. The formula of the soil heavy metal biological fixing agent is that every one kilogram of an organic fertilizer is added with 2-10 g (109 CFU/g) of B. natto solid leavening agent, 2-10 g (109 CFU/g) of B.mucilaginosus solid leavening agent, 2-6 g (109 CFU/g) of B.megaterium solid leavening agent and 4-10 g (1010 CFU/g) of Trichoderma koningii solid leavening agent, wherein the main ingredients of the biological organic fertilizer contain more than 40% of organic matter, more than 20 million/g of effective viable count, and more than 5% of total nutrient. The soil heavy metal biological fixing agent is low in cost, has few environmental risks, remarkably reduces the absorption of the heavy metals of the crops, improves the soil fertility, effectively prevents and cures the soil-borne diseases and increases the economic benefit of the crops.

The invention relates to a trichoderma pseudokoningii (SMF2) strain and application thereof, which belong to the technical field of microbiology. The invention discloses the trichoderma pseudokoningii (SMF2) strain preserved in China Center for Type Culture Collection on February 24, 2009 with the preservation number being CCTCCNO: M209031, and the application of the strain to preparation of soil microbial agents of trichoderma preparations. The trichoderma pseudokoningii (SMF2) strain disclosed by the invention can be used for producing plant disease fungus cell wall digestive enzymes (CWDEs) and antibiotics of peptaibols at high yield, the CWDEs and the peptaibols cooperate in situ, and the inhibition effect of the trichoderma pseudokoningii strain to the plant disease fungus is further enhanced.

The invention relates to an environment-friendly straw fermenting agent. The environment-friendly straw fermenting agent is prepared from the following seven raw materials in percentage by weight: 25 percent of bacillus subtilis, 15 percent of trichoderma viride, 18 percent of saccharomyces cerevisiae, 12 percent of aspergillus niger, 10 percent of streptomyces hygroscopicus, 10 percent of trichoderma koningii and 10 percent of arthrobacter. The environment-friendly straw fermenting agent can accelerate the decomposition speed of cellulose and can completely decompose crop straws within aboutten days to return to the field, so that the straws are corroded, blackened and composted. The environment-friendly straw fermenting agent can accelerate release of nutrient elements in the straws, provides more nutrients for growth of the crops at the later stage, maintains and improves soil fertility, improves soil organic matter, improves physicochemical property of the soil, increases the yield of the crops by about 10 percent, and can be widely applied to returning straws of the crops such as rice, wheat, corn and the like to the field.

The invention relates to a straw decomposing inoculant which comprises the following components of 0.2-1.5 billion/g of bacillus subtilis, 0.2-1.5 billion/g of bacillus licheniformis, 20-150 million/g of thermophilic sporotrichosis, 20-150 million/g of Trichoderma Koningii and 20-150 million/g of Phanerochete chrysosporium. Therefore, the straw decomposing inoculant has the advantages of low cost and short decomposing time and is practical and easy to operate.

The invention discloses a straw starter. The straw starter comprises Coprinus sterqulinus, penicillium expansum, trichoderma koningii and Coprinopsis atramentaria. The straw starter can be utilized for microbial fermentation of multiple straws of wheat straws and rice straws, is safe and nontoxic, has strong fermentation condition adaptability, allows a wide temperature range, and can be utilizedfor fermentation at an outdoor temperature of 15 to 35 DEG C. Based on different temperatures, fermentation time is in a range of 20 to 80 days. Straws fermented by the straw starter can be utilized as matrixes of biogas, forage and organic fertilizer.

The invention provides a preparation method for a grape seed proanthocyanidin extract. The preparation method comprises the following steps: firstly, preparing trichoderma koningii and aspergillus niger direct vat set; and secondly, adding water into grape seeds for grinding; adding glucose for mixing, sterilizing at high temperature, inoculating the bacteria and fermenting, centrifugally removing slag, carrying out ultrafiltration extraction, drying and other processes to obtain the grape seed proanthocyanidin extract.

The invention relates to a barton fermentation bed complex microbial inoculant and a preparation method thereof. The invention is characterized in that the complex microbial inoculant comprises the following six beneficial microorganisms in percentage by weight: 15-30 percent of Trichoderma koningii, 10-25 percent of geotrichum candidum, 10-25 percent of Candida utilis, 10-30 percent of lactobacillus plantarum, 10-25 percent of bacillus subtilis and 5-15 percent of bacillus subtilis natto. The complex microbial inoculant is prepared by a fermentation process, and the total number of fermentation viable bacteria in finished products is 2-5*10<9>cfu/ml. The microbial strains adopted by the complex microbial inoculant are safe and reliable, the finished viable bacteria content is high and the bioactivity is high; 1L/m<3> complex microbial inoculant is used on a barton fermentation bed, thus obviously reducing the odor in a barton, decreasing the breeding of mosquitoes and flies, having stronger degradation capability on chicken excrement, achieving the ecologically breeding purposes of no pollution and zero emission, enhancing the digestive absorption function of chicken, improving the chicken immunity, enhancing the broiler quality, increasing the quality of eggs, and ensuring the color of yolks to be brightly red.

The invention belongs to the technical field of microbe application and discloses a microbial agent for promoting quick decomposition of agricultural wastes, prepared from the following steps of respectively carrying out first stage amplification culture and second stage amplification culture on four microbes which are bacillus subtilis, micromonospora streptomyces, trichoderma koningii and chaetomium globosum, and respectively obtaining four liquid cultures of the microbes; then commonly inoculating the four liquid cultures in a carrier culture medium according to a ratio with the inoculation amount of 0.2 percent, controlling the moisture content at 55-60 percent, culturing for 7 days at a constant temperature of 28 DEG C; and finally, carrying out low-temperature drying treatment on a complex bacteria subjected to culturing at the temperature of 35-40 DEG C, and carrying out live bacteria detection. The microorganism composting microbial agent can promote quick decomposition of agricultural waste aerobic composts, has remarkable action of eliminating malodorous gas generated by organic wastes and also reduces the nutrient loss caused by volatilization in a composting process.

The invention relates to the technical field of biological agricultural chemicals, in particular to a decomposing agent for organic materials and a preparation method thereof. The decomposing agent is prepared from seed solution and a fermentation medium for co-tank fermentation. The seed solution comprises, by weight, 2-5% of acetobacter pasteurianus, 8-12% of bacillus subtilis, 8-12% of bacillus licheniformis, 3-7% of bacillus amyloliquefaciens, 8-12% of bacillus natto, 13-17% of thermophilic sporotrichum, 3-7% of photosynthetic bacteria, 4-8% of thermophilic nocardia, 3-7% of trichoderma koningii, 2-5% of saccharomyces cerevisiae, 5-8% of lactobacillus plantarum, 3-7% of rhizopus formosensis, 2-5% of rice koji, 1-3% of aspergillus niger and 8-12% of bacillus megaterium. The process includes steps of early strain culture, co-tank fermentation and solid powder preparation, and the decomposing agent contains various types of bacterial agents in synergistic effect and is effective in material degradation. The preparation method is simple in procedure, convenient to operate, easy to implement and low in production cost, bacterial antagonism is avoided, and excellent decomposing effects and high practicality are achieved.

The invention relates to a method for producing an organic fertilizer from traditional Chinese medicine dregs, which comprises the following steps: by taking traditional Chinese medicine dregs, corn cobs and clay as raw materials and wood dust as a fermentative bacterium solution carrier, pulverizing, mixing, fermenting, drying, and screening, wherein a mixed fermentative bacterium I used for primary fermentation is a mixed fermentative bacterium solution which is obtained by culturing Bacillus subtilis and Bacillus stearothermophilus according to a mass ratio of 1:1; and a mixed fermentative bacterium II used for secondary fermentation is a mixed fermentative bacterium solution which is obtained by culturing Bacillus lateraporus, Bacillus megaterium and Trichoderma koningii according to a mass ratio of 1:1:1. The invention has the following advantages: (1) the traditional Chinese medicine dregs are fully utilized, and the wastes are changed into valuable substances; (2) the addition of attapulgite powder can promote the fermentation process, and is beneficial to the shaping and granulation of fermentation finished products; (3) through the fermentation treatment, pathogens in the raw materials are eliminated; and (4) the organic content in the obtained organic fertilizer is higher than 55%, the NKP content exceeds 4%, the soil property can be effectively improved, and the crop yield can be increased.

The invention discloses a production method utilizing cassava stems, cassava residues and pasture to manufacture a biological feed for beef cattle. The production method comprises the following steps: (1) crushing cassava stems, cassava residues and pasture with a crusher, screening, and taking particles of 2-10 meshes; (2) adding urea of which the weight is 1% of that of the particles into the particles, uniformly mixing, then processing with steam of 120-125 DEG C for 30-60 min, cooling to the temperature below 30 DEG C, adding a green adding a green trichoderma liquid culture solution and cultivating for 24-48 h; (3) adding the material obtained from the step (2) into a trichoderma koningii liquid culture solution and cultivating for 24-48 h; (4) adding the material obtained from the step (3) into a lactic acid bacteria liquid culture solution, and sealing for at least 7 days to obtain the biological feed for beef cattle.

The invention provides a complex bacteria inoculant for preparing an organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials, which has the advantages of effectively deodorizing and enhancing the fertility, and a preparation method thereof. The complex bacteria liquid inoculant is prepared by the following steps of: independently performing liquidfermentation on each single bacterium, namely A, lactobacillus fermentium, B, bacillus laterosporus, C, trichoderma koningii, D, nitrogen-fixing bacteria spores and E, bacillus mucilaginosus; and mixing the bacteria, wherein the final product comprises the living bacteria (including living spores) in percentage by weight: 20 to 50 percent of A, 20 to 50 percent of B, 0 to 20 percent of C, 0 to 20percent of D and 0 to 20 percent E. The liquid inoculant is further processed so as to obtain solid inoculant. The complex inoculant of the invention is specially used for preparing the organic fertilizer from excrements of pig farms (or chicken farms) and wastes of slaughter houses thereof, which serve as basic raw materials, and a small amount of straws, and has an obvious effect of reducing odor of the fertilizer and the field. The complex inoculant combines the functions of improving the disease resistance of plants, promoting growth and improving soil and the fertility of the fertilizer.

The invention provides trichoderma koningii with a collection number CGMCC No.9726. The invention also provides a compound microorganism bacterial agent; and the active ingredients of the compound microorganism bacterial agent include the trichoderma koningii, bacillus subtillis and bacillus amyloliquefaciens. The invention also provides application of the trichoderma koningii or the microorganism bacterial agent in the prevention of cylindrocarpon destructans. Through the technical scheme, the trichoderma koningii and the compound microorganism bacterial agent can effectively prevent cylindrocarpon destructans.

The invention discloses a straw decomposing agent and a preparation method thereof. The preparation method comprises the following steps: culturing bacillus subtilis, bacillus licheniformis, liquor-making yeasts, aspergillus niger and trichoderma koningii respectively until the bacterial concentration is (0.1-1)*10<8>/mL, uniformly mixing according to a bacterial amount ratio, inoculating to sugarcane leaves according to a ratio of 1 to (10-20) for culturing at 25-30 DEG C for 5-10 days under the condition that the moisture content of the sugarcane leaves is 30%-50%, and finally grinding the sugarcane leaves to be 20-100-mesh to prepare the straw decomposing agent. According to the preparation method, the bacterial selection and ratio are reasonable, and the bacteria have a synergistic effect, can effectively decompose ingredients such as cellulose, hemicelluloses and lignin in straws and increase the decomposing speed, and can effectively decompose the straws within 15 days in general; the preparation method is simple and easy to control, the culture is mainly carried out at the room temperature, a high-price culture agent does not need to be added, the production cost can be reduced, and the promotion is facilitated.

The invention discloses a method for preparing rice hull ash active carbon from solid-state co-fermentation rice hull. The method comprises the steps of: firstly, incubating white-rot fungi in a rice hull fermentation culture medium, incubating trichoderma viride, or aspergillus niger or trichoderma reesei or trichoderma koningii or blue mould, and carrying out dry distillation to prepare the rice hull ash active carbon after solid-state co-fermentation. The method is simple in preparation process, and easy to operate; and the prepared rice hull ash active carbon has a good surface micro-pore structure and strong adsorptive property.

The invention belongs to the forage processing field, and relates to a making method of a tomato pomace fermented forage. The making method of the fermented forage comprises the following steps: uniformly stirring fermentation raw materials (a DM base) comprising 65-75% of a tomato pomace, 10-20% of wheat bran, 8-15% of cottonseed meal, 1.5-2.5% of urea and 1.5-2.5% of ammonium sulfate; inoculating Trichoderma koningii, Candida utilis and Saccharomyces cerevisiae according to a ratio of the Saccharomyces cerevisiae to the Candida utilis to the Saccharomyces cerevisiae of 1:1:1-1:2:2, wherein the inoculation amount accounts for 10-15% of a fermentation substrate, and the water content of the fermentation substrate is 45-55% (m/m); and carrying out shallow layer fermentation or submerged fermentation at 28-32DEG C for 2-4d while stirring once each 24h. The tomato pomace fermented forage can be directly used to feed ruminants, or is baked at 45-55DEG C to the water content of about 10% in order to obtain a tomato pomace fermented forage product. The fermentation method has the advantages of small energy consumption, short period and low cost, and the produced tomato pomace fermented forage has a crude protein content of 26% or more (the DM base) and a urea residual amount of 0.1% or less. The fermented forage has a good palatability and can be used for feeding the ruminants.

The invention discloses a method for extracting cannabidiol by using microbial fermentation, and belongs to the technical field of chemical drug intermediate extraction. The method comprises the stepsof drying, smashing and sieving flowers and flower leaves of industrial hemps to obtain industrial hemp powder; adding water into the industrial hemp powder to obtain a mixed solution, and inoculating mixed bacteria of paecilomyces hepiali, trichoderma koningii and aspergillus niger into the mixed solution for fermentation to obtain a fermentation solution; adopting a three-phase separation machine for conducting three-phase separation on the fermentation solution to obtain an aqueous phase, a residue and an oil phase; concentrating the oil phase under reduced pressure to obtain a CBD-enriched extract. The method is high in extraction rate and suitable for industrial production.

The invention discloses an organic fertilizer fermentation inoculant and application. The inoculant is composed of a primary fermentation inoculant body and a secondary fermentation inoculant body. The primary fermentation inoculant body comprises bacillus subtilis, bacillus licheniformis, trichoderma viride, aspergillus oryzae, pseudomonas, trichoderma koningii, geotrichum candidum, long spiny shells, ascomycetes, thermomyces lanuginosus and thermobifida fusca. The secondary fermentation inoculant body comprises halophilic bacteria, alkalophilic bacteria, azotobacter chroococcum, bacillus megatherium, streptomyces griseus, bacillus thuringiensis, brevibacillus laterosporus and bacillus mucilaginosus. Time for fermenting organic fertilizer with cow dung as the raw material can be effectively shortened, and diseases and insect pests in the fertilizer can be eliminated. Meanwhile, alkalophilic bacteria and bacillus thuringiensis are added so that the fermentation inoculant can adapt to saline-alkali soil and can prevent diseases and insect pests of plants, and the prepared organic fertilizer can improve the soil structure, reduces the salt content and the pH value in soil, improves soil fertility and is particularly beneficial to improvement of saline-alkali soil.

The invention relates to the technical field of microbes, and specifically relates to trichoderma koningii, and a compound microbial agent composition and an application thereof. The invention discloses trichoderma koningii HK43-5 (CGMCC No7422), a compound microbial agent composition composed of a glycosylated bacterial preparation and a forage yeast preparation which are packaged separately, wherein the glycosylated bacterial preparation comprises the trichoderma koningii HK43-5 (CGMCC No7422). Cellulose produced by the trichoderma koningii HK43-5 (CGMCC No7422) has high activity, can be used as an efficient glycosylated bacterial preparation in production of industrial and agricultural residual protein forage. The compound microbial agent composition can efficiently utilize industrial and agricultural residues, particularly potato residues, to produce single cell protein. Crude protein content in fermented mycoprotein is 50.49% which is far superior to that reported in a conventional technology. The industrial and agricultural residues are changed to wealth; and the produced bacterial protein can be applied in the forage to partly substitute soybean meal or fish meal. At the same time, the environment pollution problems resulted from the industrial and agricultural residues are solved.