Method for extracting cannabidiol by using microbial fermentation
A microbial fermentation, cannabidiol technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as low efficiency, large equipment investment, residues, etc., and achieve high efficiency, low equipment requirements, and conditions. mild effect
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Embodiment 1
[0019] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:
[0020] Step 1: drying the flowers and leaves of industrial hemp at 55°C in vacuum and low temperature until the water content is below 5.0%, crushing and passing through a 120-mesh sieve to obtain industrial hemp powder;
[0021] Step 2: add water to the industrial hemp powder according to the mass ratio of industrial hemp powder and water to 1:8 to obtain a mixed solution, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.35% (mass). Bacteria were fermented for 40 hours at a temperature of 32°C and a pH of 6.2 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;
[0022] Step 3: using a three-phase separator to perform three-phase separation on the fermentation broth to...
Embodiment 2
[0026] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:
[0027] Step 1: drying the flowers and leaves of industrial hemp under vacuum at 65°C until the water content is below 5.0%, crushing and passing through a 200-mesh sieve to obtain industrial hemp powder;
[0028] Step 2: add water to the industrial hemp powder according to the mass ratio of the industrial hemp powder and water at 1:10 to obtain a mixed solution, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.45% (mass). Bacteria were fermented for 48 hours at a temperature of 36°C and a pH of 7.0 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;
[0029] Step 3: using a three-phase separator to perform three-phase separation on the fermentation broth to obtain an a...
Embodiment 3
[0033] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:
[0034] Step 1: drying the flowers and leaves of industrial hemp at 62°C in vacuum and low temperature until the moisture content is below 5.0%, crushing and passing through a 116-mesh sieve to obtain industrial hemp powder;
[0035] Step 2: add water to the industrial hemp powder according to the mass ratio of industrial hemp powder and water to obtain a mixed solution of 1:8.5, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.42% (quality) Bacteria were fermented for 46 hours at a temperature of 35°C and a pH of 6.8 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;
[0036] Step 3: using a three-phase separator to perform three-phase separation on the fermentation b...
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