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Method for extracting cannabidiol by using microbial fermentation

A microbial fermentation, cannabidiol technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as low efficiency, large equipment investment, residues, etc., and achieve high efficiency, low equipment requirements, and conditions. mild effect

Inactive Publication Date: 2019-05-21
黑龙江阳光工业大麻研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the existing cannabidiol extraction method has solvent residues in the heavy solvent extraction method and the supercritical extraction technology has no solvent residues, but the equipment investment is large and the efficiency is low, the invention provides an enzymatic method combined with a membrane method to extract cannabis The method of diphenol, this method has no solvent residue, and with low cost, the technical scheme that adopts is as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:

[0020] Step 1: drying the flowers and leaves of industrial hemp at 55°C in vacuum and low temperature until the water content is below 5.0%, crushing and passing through a 120-mesh sieve to obtain industrial hemp powder;

[0021] Step 2: add water to the industrial hemp powder according to the mass ratio of industrial hemp powder and water to 1:8 to obtain a mixed solution, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.35% (mass). Bacteria were fermented for 40 hours at a temperature of 32°C and a pH of 6.2 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;

[0022] Step 3: using a three-phase separator to perform three-phase separation on the fermentation broth to...

Embodiment 2

[0026] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:

[0027] Step 1: drying the flowers and leaves of industrial hemp under vacuum at 65°C until the water content is below 5.0%, crushing and passing through a 200-mesh sieve to obtain industrial hemp powder;

[0028] Step 2: add water to the industrial hemp powder according to the mass ratio of the industrial hemp powder and water at 1:10 to obtain a mixed solution, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.45% (mass). Bacteria were fermented for 48 hours at a temperature of 36°C and a pH of 7.0 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;

[0029] Step 3: using a three-phase separator to perform three-phase separation on the fermentation broth to obtain an a...

Embodiment 3

[0033] This embodiment provides a method for extracting cannabidiol (CBD) by microbial fermentation, which is carried out according to the following steps:

[0034] Step 1: drying the flowers and leaves of industrial hemp at 62°C in vacuum and low temperature until the moisture content is below 5.0%, crushing and passing through a 116-mesh sieve to obtain industrial hemp powder;

[0035] Step 2: add water to the industrial hemp powder according to the mass ratio of industrial hemp powder and water to obtain a mixed solution of 1:8.5, and inoculate the mixture of Penicillium, Trichoderma and Aspergillus niger in the mixed solution according to the inoculation amount of 0.42% (quality) Bacteria were fermented for 46 hours at a temperature of 35°C and a pH of 6.8 to obtain a fermentation broth; wherein the mass ratio of Penicillium, Trichoderma and Aspergillus niger was 2:2.3:5;

[0036] Step 3: using a three-phase separator to perform three-phase separation on the fermentation b...

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PUM

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Abstract

The invention discloses a method for extracting cannabidiol by using microbial fermentation, and belongs to the technical field of chemical drug intermediate extraction. The method comprises the stepsof drying, smashing and sieving flowers and flower leaves of industrial hemps to obtain industrial hemp powder; adding water into the industrial hemp powder to obtain a mixed solution, and inoculating mixed bacteria of paecilomyces hepiali, trichoderma koningii and aspergillus niger into the mixed solution for fermentation to obtain a fermentation solution; adopting a three-phase separation machine for conducting three-phase separation on the fermentation solution to obtain an aqueous phase, a residue and an oil phase; concentrating the oil phase under reduced pressure to obtain a CBD-enriched extract. The method is high in extraction rate and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting cannabidiol by microbial fermentation, and belongs to the technical field of extraction of chemical drug intermediates. Background technique [0002] The extraction methods of existing cannabidiol (CBD) are mainly solvent extraction and supercritical extraction. However, there is a problem of solvent residue in the cannabidiol extracted by the solvent extraction method. For example, the invention patent application ZL201410052776.8 discloses a method for preparing hemp extract rich in CBD by soaking hemp flower leaves in a solvent such as n-hexane. The yield of hemp extract prepared by this technology is not high, and a large amount of solvent is used, and the yield and content of CBD are not indicated; while the supercritical extraction technology has a higher extraction rate and no solvent residue, but the equipment investment is large and the efficiency is relatively low. Low, PCT application (WO00 / 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/68C07C37/86C07C37/72C07C37/82C07C39/23
Inventor 曹亮刘欣黄莉黄倩
Owner 黑龙江阳光工业大麻研究院
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