89 results about "Paecilomyces hepiali" patented technology

The invention discloses a ghost moth paecilomyces leavening feed additive. Ghost moth paecilomyces leavening is obtained in such a way that myceliums of a high-yield bacterial strain obtained by mutagenizing ghost moth paecilomyces separated from worm grass by ion beams are dried and crushed together with a a culture medium after strain preparation, shaking culture, primary starter can fermentation, fermentation can fermentation, solid culture fermentation. The invention also discloses an application of the ghost moth paecilomyces leavening feed additive in the preparation of animal feed, and the ghost moth paecilomyces leavening feed additive is added in the animal feed by 0.05 to 0.5 percent . The ghost moth paecilomyces leavening feed additive can increase the daily gain of animals, obviously promote the growth of the animals, improve the conversion rate of the feed, enhance the production performance and the antiviral capability of the animals and can also improve the quality of livestock and fowl meat. The ghost moth paecilomyces leavening has simple preparation technology flows, simple-formula culture medium and cheap and easily obtained raw materials.

The invention relates to a health care food containing fermentation cordyceps and lucid ganoderma, capable of enhancing organism immunity. Paecilomyces hepiali Chen and ganoderma lucidum karst are respectively subjected to liquid deep fermentation to obtain mycelium and fermentation liquor, then spray rotary fluidization is carried out for dry pelleting, and the diameter is controlled at 500-800mu m. The ratio of cordyceps fermentation powder and lucid ganoderma fermentation powder according to parts by weight is 1:1-4:1. The content of cordycepin in the product is greater than or equal to 1.5mg / g, the content of polysaccharide from cordyceps is greater than or equal to 2.0mg / g, the content of adenoside is greater than or equal to 4.0mg / g, and the content of polysaccharide from lucid ganoderma is greater than or equal to 1.5mg / g. The invention uses spray rotary fluidization for dry pelleting, can fully retain fermentation liquor and mycelium in the product without concentration and extraction, has simple technology, avoids wasting nutrient, generates no waste liquor and waster residue, and has evenly distributed particles of the finished product and excellent intensity and solvability. The health care food of the invention has obvious effects on deficiency of the kidney, deficiency of vital energy, deficiency of the lung and cough, and has the functions of strengthening the bones and muscles, dispelling fatigue, calming the nerves, resisting tumour and the like.

The invention discloses a health food for alleviating physical fatigue and a preparation process of the health food. The health food is prepared from Chinese caterpillar fungus (Paecilomyces hepiali) fungus powder, ganoderma lucidum extract and wall-breaking spore powder. The formula of the capsule is as follows: 50-55% of Paecilomyces hepialid, 25-30% of ganoderma lucidum extract and 20-25% of wall-breaking spore powder, wherein the triterpene content of a final product is not less than 0.6%, and the adenosine content is not less than 0.12%, the three raw materials make up for deficiencies with one another on efficacies and complement mutually, and animal experiments prove that the raw materials have the efficacy of alleviating physical fatigue. The newly harvested ganoderma lucidum spore powder adopts a process of combining a gradient temperature rise and a tilting pan in a drying process to avoid surface dehydration and shrinkage of materials in a traditional thermostatic drying process, so as to result in a difficult escape of water inside the materials to cause the defect of prolonging a drying time.

The invention discloses a method for extracting cannabidiol by using microbial fermentation, and belongs to the technical field of chemical drug intermediate extraction. The method comprises the stepsof drying, smashing and sieving flowers and flower leaves of industrial hemps to obtain industrial hemp powder; adding water into the industrial hemp powder to obtain a mixed solution, and inoculating mixed bacteria of paecilomyces hepiali, trichoderma koningii and aspergillus niger into the mixed solution for fermentation to obtain a fermentation solution; adopting a three-phase separation machine for conducting three-phase separation on the fermentation solution to obtain an aqueous phase, a residue and an oil phase; concentrating the oil phase under reduced pressure to obtain a CBD-enriched extract. The method is high in extraction rate and suitable for industrial production.

The invention discloses a composite health food for alleviating the physical fatigue and a preparation method of the composite health food. The composite health food comprises maca powder, paecilomyces hepialid, extract of the fruit of Chinese wolfberry and L-arginine hydrochloride. The invention further discloses the preparation method of the composite health food. Animal experiments prove that the composite health food has the function of alleviating the physical fatigue without any toxic and side effects.

The invention discloses fermented cordyceps sinensis mycelium powder and a preparation method thereof. The preparation method comprises the following steps of performing inoculation with paecilomyces hepiali strain, performing constant-temperature culture, performing culture in a seeding tank, performing culture with a fermenter, performing ultralow temperature vacuum concentration, performing freeze drying and performing crushing. Compared with cordyceps sinensis mycelium powder produced in the patent of which the application number is 201310124056.3, the cordyceps sinensis mycelium powder made by the method disclosed by the invention has the advantages that the content of calcium in the cordyceps sinensis mycelium powder is increased by 15-20%, the content of magnesium is increased by 5-8%, the content of iron is increased by 5-7%, the content of zinc is increased by 6-9%, the content of cordycepic acid is increased by 5-8%, the content of cordycepin is increased by 6-10%, and the activity of SOD enzyme is improved by 4-7%. The invention further discloses an application of using the cordyceps sinensis mycelium powder as an active component in preparing a preparation for improving the immune function of organisms.

The invention relates to a method for cultivating caterpillar fungus by using a ginseng type extract and application. According to the method, in a caterpillar fungus seed cultivation and fermentation cultivation process, the ginseng type extract is added into a culture medium for continuous cultivation and fermentation according to a conventional caterpillar fungus process. The caterpillar fungus can be agamotype Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tremellaceae, Oznyun sp or paecilomyces hepialid. The cultivation method provided by the invention is simple and convenient; the quantity of the needed ginseng extracts is small, and the cost is low; mycelium of a strain cultivated by the cultivation method is strong and can grow fast; the content of cordycepin in the mycelium obtained by fermentation is high; fermentation liquid and the mycelium which are cultivated by the method can be used for producing health-care caterpillar fungus products.

The invention relates to a compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine, which is characterized in that: the medicine comprises the following raw material components in part by weight: 0.30 to 0.70 part of fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder), 0.45 to 0.90 part of disodium edetate, 0.05 to 0.10 part of ligustrazine phosphate, and 5.0 to 10.0 parts of water for injection (completely volatilized when dried); a preparation method of the compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine comprises the steps shown in the specifications; the compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine can be prepared into capsules, granules or tablets according to pharmaceutically acceptable dosage; and the combined medicine has new application, namely is used for resisting ageing, fatigue, shock and anoxia and improving immunity.

The invention relates to a fermentation production technology of paecilomyces hepiali Cs-4. The fermentation production technology comprises slant culture, shake-flask culture, seed tank culture, reproduction tank culture and fermentation tank culture; a fermentation solution obtained by fermentation can be used as seeds of the shake-flask culture. A slant culture medium contains histidine; a shake-flask culture medium contains the histidine and vitamin B1; a seed tank culture medium contains purine; a reproduction tank culture medium contains purine and pyrimidine; a fermentation tank culturemedium contains purine and pyrimidine; bacterium powder obtained by culture has high nucleoside content. After radix notoginseng powder is added into a liquid fermentation culture medium, prepared caterpillar fungus powder contains saponin and the content of ergosterol is also improved. In the shake-flask culture process, red light irradiation is carried out and the content of active components in the bacterium powder can be improved. The fermentation production technology provided by the invention has the advantages of simplicity in operation, short fermentation period and low cost and is suitable for industrial large-scale production.

The invention discloses paecilomyces hepiali producing coremia on silkworm pupae and a culture method thereof. The paecilomyces hepiali is particular paecilomyces hepiali DCH-60, the preservation number in the China General Microbiological Culture Collection Center is CGMCC No.10901. According to the paecilomyces hepiali producing the coremia on the silkworm pupae and the culture method thereof, the paecilomyces hepiali DCH-60 is obtained by being separated from natural cordyceps sinensis, culture is conducted on the silkworm pupae, and the coremia are obtained, and the paecilomyces hepiali producing the coremia on the silkworm pupae is expected to serve as health-care food or medicine to be developed.

The invention discloses a medical composition for treating type 1 or type 2 diabetes mellitus and a preparation method thereof. The medical composition is prepared from a solid or liquid fermentation culture of paecilomyces hepiali.

Methods for stimulating in-vitro paecilomyces hepiali or hirsutella sinensis growth, and nutritional supplement formulations containing such fungal strains are disclosed and described. In some embodiments, such a method may include providing a an effective amount of a p. hepiali fungus strain for use as a stimulating agent on a culture medium for which h. sinensis is grown and regulating the temperature to induce and maintain the growth of the h. sinensis fungus. Alternatively, a p. hepiali fungus may be grown in a similar manner using h. sinensis as the stimulating agent. In some aspects, the growth of one fungus may be sequentially switched with the other in order to improve fungi potency.

The invention discloses a specific PCR (Polymerase Chain Reaction) identification method of paecilomyces hepiali powder. The technical scheme is that the method comprises the following steps: by taking a genome DNA (Deoxyribonucleic Acid) of a sample as a template, carrying out a primary single tube specific PCR reaction by adopting a specific PCR primer designed by the invention; amplifying a characteristic housekeeping gene of winter paecilomyces hepialid, wherein the actual amplification segment is 231bp; and detecting the amplification products by agarose gel electrophoresis to judge the authenticity of the sample. The method disclosed by the invention can specifically identify the paecilomyces hepiali powder, and can detect the authenticity of the sample just by three steps: DNA extraction, PCR amplification and electrophoretic detection, so that the method is simple to operate, easy to master and high in accuracy.

The invention discloses weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine and, weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine, weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine, as well as the application of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine, the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine and the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine for anti-aging, antifatigue, anti-hypoxia, immunity enhanced, sedative, antishock and anti irradiation.

The invention discloses a composite fermentation beverage and a preparation method of the composite fermentation beverage. The composite fermentation beverage is prepared from the following ingredients in parts by mass: 250 to 350 parts of ginkgo extracting solution, 100 to 200 parts of soybean peptides extracting solution, 100 to 200 parts of burdock extracting solution, 500 to 700 parts of apple juice, 100 to 120 parts of black maca extracts, 80 to 100 parts of grape skin extracting solution, 60 to 80 parts of paecilomyces hepialid mycelium extracting solution, 30 to 50 parts of mixed bacterium suspension, 40 to 80 parts of white granulated sugar and 2 to 3 parts of stabilizing agents. A product is obtained through proportioning, fermentation, ultrasonic wave treatment, filtering, homogenizing, degassing, filling, packaging and sterilization. The obtained product is rich of substances beneficial to the human bodies, such as flavone, polysaccharide, polypeptide, oligosaccharide, procyanidine and vitamin, and integrates the health care and the nutrition. The ultrasonic waves are used for being cooperated with the chitosan, the pectin and the xanthan gum to improve the beverage stability; the method is simple; the cost is low; no toxic or side effect exists.

The present invention relates to novel and non-obvious methods and media for preparing fermentation products of Cordyceps sinensis. The present invention also relates to novel and non-obvious compositions comprising fermentation products of Cordyceps sinensis produced by the methods of the invention or obtained from other sources. The present invention also relates to novel and non-obvious methods of treating patients by administering the compositions of the invention. In one embodiment, the Cordyceps sinensis mycelia is Paecilomyces hepiali mycelia. In another embodiment the compositions of the invention are used to treat patients infected with hepatitis C.

The invention relates to a method for culturing cordyceps sinensis by utilizing astragalus membranaceus and an application thereof. The method comprises the following steps of: in the culture and / or fermentation process of cordyceps sinensis seeds, adding the astragalus membranaceus into a culture medium of the cordyceps sinensis, and continuously culturing and fermenting according to the conventional process of the cordyceps sinensis, wherein the preferred cordyceps sinensis is anamorph Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tolypocladium sinense, synnema or paecilomyces hepiali. The method provided by the invention is simple and convenient, the needed astragalus membranaceus extract is less and the cost is low; and the strain is fast in growth and excellent in quality, and the obtained fermentation liquid and mycelia are high in cordycepin content. The fermentation liquid and mycelia cultured by the method can be used for producing cordyceps sinensis health-care products.

The invention relates to the technical field of traditional Chinese medicine health-care products, in particular to a trepang, hippophae rhamnoides fruit, cordyceps militaris and selenium preparation and a preparation technology thereof. The preparation is characterized by consisting of the following components in parts by weight of 20-30 parts of trepang powder, 10-25 parts of pseudo-ginseng, 25-60 parts of hippophae rhamnoides fruit powder, 20-30 parts of radix astragali, 10-25 parts of dendrobium nobile stem powder, 10-20 parts of cordyceps militaris, 5-15 parts of chitosan oligosaccharide, 10-18 parts of glucan, 10-22 parts of paecilomyces hepiali powder, 15-30 parts of a ginger extract and 0.01-0.05 part of selenium. The trepang, hippophae rhamnoides fruit, cordyceps militaris and selenium preparation has the advantages of improving the immunity of human bodies, promoting blood circulation, supplementing qi, strengthening constitutions of human bodies, resisting radiation, delaying aging, improving memory and the like.

The invention discloses a detection primer group for Paecilomyces hepiali Chen et Dai in an ophiocordyceps sinensis culture solution, a detection kit and a detection method. Genomic DNA of the ophiocordyceps sinensis culture solution is taken as a template, two times of single-tube specific PCR (polymerase chain reaction) are performed by adopting the designed detection primer group, an amplification product is detected through agarose gel electrophoresis, and the existence condition of Paecilomyces hepiali Chen et Dai in the ophiocordyceps sinensis culture solution is judged. According to theinvention, the carrying condition of Paecilomyces hepiali Chen et Dai in the ophiocordyceps sinensis culture solution is detected through the conventional PCR, sample detection can be finished only through three steps including DNA extraction, PCR amplification and electrophoresis analysis, the method is simple to operate, easy to master and high in accuracy, and the detection kit can be furtherdeveloped with the method. The new method is provided for excluding the ophiocordyceps sinensis culture solution carrying Paecilomyces hepiali Chen et Dai, and technical support is provided for increase of infection rate of ophiocordyceps sinensis for hepialus larvae as well as stroma formation of the ophiocordyceps sinensis.

The invention relates to the field of traditional Chinese medicines, particularly to a traditional Chinese medicine preparation with immunity enhancing function, and a preparation method thereof, wherein the traditional Chinese medicine preparation is prepared from the following raw materials by weight: 3-5 parts of Paecilomyces hepiali mycelium, 4-7 parts of the extract of Rhodiola rosea L, 5-8 parts of the extract of ganoderma lucidum, 3-7 parts of the extract of American ginseng, and 10-16 parts of the extract of tuckahoe. According to the present invention, the traditional Chinese medicinepreparation contains the five traditional Chinese medicine raw materials, wherein the five raw materials synergistically act so as to balance and regulate various functions of the body, enhance the human immunity in all directions, and enhance the anti-oxidation capacity and the anti-fatigue ability of the human body; a variety of the dosage forms can be prepared, such that the clinical use is convenient; the traditional Chinese medicine preparation completely adopts the natural Chinese herbs, is the pure natural health product, and has characteristics of no toxicity, no harm and no side effects; and the preparation method has characteristics of simpleness, easy performing and easy promoting.

The invention discloses a technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth. The technology comprises steps of pulping, water bath extraction, centrifugation, membrane separation and vacuum drying. In the water bath extraction step, the material-water ratio of a thallus breaking liquid to distilled water is (1:1)-(3:1) in volume, the water bath temperature is 85-95 DEG C, and the extraction time lasts for 4-8 h. In the pulping step, the rotating speed of pumping is 16,000-22,000 r / min, and pulping is performed for 1-2 min in every other 1-2 min and performed 2-12 times. The mass percentage of the cordyceps polysaccharide is 90% or higher.

The invention discloses a method for increasing adenosine content in a paecilomyces hepiali fermentation mycelium. The method comprises the steps of strain activation, seed liquid preparation, fermentation with a fermentation tank and extraction, and a fermentation medium adopted in the fermentation step comprises 20-25 g / L of soybean cake powder, 10-15 g / L of glucose, 10-15 g / L of sucrose, 0.3-0.5 g / L of potassium dihydrogen phosphate, 0.2-0.4 g / L of MgSO4. 7H2O, 0.25 g / L of anhydrous calcium chloride, 0.4 g / L of soybean oil, 0.02-0.1 g / L of inosine monophosphate (IMP) and the balance of water. The IMP is added into the fermentation medium, so that the adenosine content in the paecilomyces hepiali mycelium powder can be increased, and the yield of the paecilomyces hepiali mycelium powdercan also be increased.

The invention belongs to the technical field of fermentation of staple medical fungi. Paecilomyces hepiali is an important substitute product of wild cordyceps sinensis, about 3000 tons of liquid fermented powder of paecilomyces hepiali is needed in China each year, the production value of the product taking the liquid fermented powder of paecilomyces hepiali as the main raw material breaks through 5 billion yuan, and animal peptone is the main nitrogen source for producing the fermented powder of paecilomyces hepiali. The invention specifically relates to a preparation method of peptone specially used for producing paecilomyces hepiali powder through submerged fermentation. The preparation method comprises the following steps: mixing pork material and beef material, carrying out high-pressure steaming, carrying out enzymolysis, carrying out concentrating, and carrying out spray drying, thereby obtaining the peptone product. Under the condition of a 10-ton fermentation tank and with the same cost, by using the peptone provided by the invention for replacing the common peptone to carry out fermentation production of paecilomyces hepiali powder, the yield and the quality of the paecilomyces hepiali powder are obviously improved.

The invention provides application of Cordyceps fungal cell metabolites in the preparation of skin-care products and a skin-care product; Cordyceps fungi herein at least include any one of Cordyceps sinensis, Hirsutella sinensis, Paecilomyces hepiali, Hirsutella hepiali, Cordyceps militaris, Cordyceps algae, Cordyceps ophioglossoides, Cordyceps hawkesii, Cordyceps martialis, Ophiocordyceps sobolifera, and Cordyceps fruit flies. The Cordyceps fungal cell metabolites herein are not typical antibiotics, but provide germ-killing action; the Cordyceps fungal cell metabolites are not typical anti-inflammatory agents, but have an inflammation diminishing function and are natural and free of side effects.

The invention belongs to the technical field of health products and specifically relates to a health-care composition for increasing bone mineral density and easing joint movement and a preparation method thereof. The health-care composition is prepared from the following raw materials: chondroitin sulfate, glucosamine, hyaluronic acid, paecilomyces hepiali mycelium, eucommia, cyathula root, epimeddium, Smilax glabra, dendrobium and Rhizoma Drynariae. The preparation method comprises the following steps: extracting and refining eucommia, cyathula root, epimeddium, Smilax glabra, dendrobium and Rhizoma Drynariae to obtain an extract powder, and mixing the extract powder with chondroitin sulfate, glucosamine, hyaluronic acid and paecilomyces hepiali mycelium. With the ten medicines complementing each other, the health-care composition has effects of tonifying kidney and reinforcing bone and easing joint movement. By synergism, the composition plays a better role in increasing bone mineral density and easing joint movement.

The invention discloses a medical application of paecilomyces hepidl chen PH40 in resisting depression, and a remarkable medical effect is achieved. Paecilomyces hepidl chen PH40 bacterial powder is adopted as main components, and multiple kinds of pharmaceutic adjuvant materials are added, thereby preparing tablets, powder, capsules and oral preparations.

The invention discloses an immune enhancement Hepialus American ginseng preparation and a capsule preparation technique thereof. The Hepialus American ginseng preparation is characterized by comprising, by weight, 240-260 parts of American ginseng, 95-110 parts of paecilomyces hepiali hyphae, 140-160 parts of radix astragali, 90-110 parts of Chinese angelica, 180-220 parts of coix seeds, 90-120 parts of radix pseudostellariae, 90-120 parts of codonopsis pilosula, 80-100 parts of northern hawthorn, 75-85 parts of orange peels, 90-120 parts of red dates and 55-65 parts of hyacinth beans. According to integrity of basic theories of Chinese medicine, loss of qi, blood, yin and yang of lung, spleen, heart, liver and kidney is tuned up by tonifying heart-lung qi deficiency, warming and boosting spleen yang, reinforcing the spleen, promoting appetite and nourishing the liver and the kidney, so that fatigue is resisted, and a body immunity function is improved. Medicine taking conditions are improved, the preparation technique is simple and feasible, and the preparation is low in dosage, convenient to take and stable and remarkable in curative effect and has no side effects.