Method for cultivating caterpillar fungus by using ginseng type extract and application

A technology of Cordyceps sinensis fungus and extract, applied in the fields of fermentation and microbial culture, can solve the problems of raising cordycepin content by fermentation of unseen ginseng, and achieve the effects of improving cordycepin content, good quality and low cost

Inactive Publication Date: 2017-03-22
锬酃藏虫草生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pharmacological studies have shown that ginseng contains a variety of saponins and polysaccharides, which have the functions of nourishing vitality, restoring pulse and solidifying, nourishing the spleen and lung, promoting body fluid and calming the nerves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Preparation of ginseng extract: take commercially purchased ginseng from Changbai Mountain in Jilin, Northeast China, remove dust and impurities, dry and pulverize, and pass through a 80-mesh sieve to obtain coarse powder. Take 300g of coarse powder, put it in an ultrasonic extractor, add 3000ml of pure water, leaching at a temperature of 75 degrees, power 300W, ultrasonic leaching for 30 minutes, filter out the leaching liquid, and add 2000ml of pure water to the leaching residue. 75 degrees, power 300W, ultrasonic leaching for 30 minutes, filter out the leachate, combine the filtrates, put in a rotary thin film concentrator, and concentrate until the residual leachate is 450ml in a water bath with a temperature of 75 degrees and a vacuum of 0.097Mpa.

[0034] The strain of Cordyceps sinensis is isolated and purified from natural Cordyceps sinensis in Nagchu, Tibet, and identified as Cordyceps sinensis by molecular biology such as strain culture characteristics, microsco...

Embodiment 2

[0049] Compared with Example 1, the difference is only that: in the case of carrying out seed parallel culture, the test group added 0.75% ginseng extract accounting for the volume of the liquid seed medium before the seed medium was inserted into the seeds, as The test group; the control group without ginseng extract.

[0050] After the fermentation, collect 1000ml of Cordyceps sinensis mycelia from the test group and the control group respectively, add 70% ethanol aqueous solution and aqueous solution at a weight volume ratio of 1:8, and extract at 55°C and 95°C for 3 times, each time for 30min; Ultrasonic extraction, filtering, and concentrating the filtrate to obtain 385ml Cordyceps oral liquid.

[0051] And measure the content of cordycepin in the oral liquid of Cordyceps sinensis of test group and control group with described high performance liquid chromatography, detection result is as shown in table 2

[0052] Table 2: Cordycepin content per 100ml oral solution

[0...

Embodiment 3

[0055] Compared with Example 1, the difference is only that: in the case of carrying out seed parallel cultivation, the test group added 0.95% of the ginseng extract accounting for the volume of the liquid seed culture medium at the late stage of exponential growth of the seed culture, as a test group; no ginseng extract was added as the control group.

[0056] After the fermentation, the Cordyceps sinensis fermentation fluids of the test group and the control group were collected respectively. Pure grain rice wine with an alcohol content of 52-56 degrees is added to the fermented liquid at a ratio of 1:1.1 by volume, processed by a homogenizer (operating pressure is 90 MPa), and aged to obtain Cordyceps pulp health wine.

[0057] And the content of cordycepin in the Cordyceps pulp health wine of the test group and the control group was determined by the high-performance liquid chromatography, and the detection results are shown in Table 3.

[0058] Table 3: Cordycepin conten...

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Abstract

The invention relates to a method for cultivating caterpillar fungus by using a ginseng type extract and application. According to the method, in a caterpillar fungus seed cultivation and fermentation cultivation process, the ginseng type extract is added into a culture medium for continuous cultivation and fermentation according to a conventional caterpillar fungus process. The caterpillar fungus can be agamotype Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tremellaceae, Oznyun sp or paecilomyces hepialid. The cultivation method provided by the invention is simple and convenient; the quantity of the needed ginseng extracts is small, and the cost is low; mycelium of a strain cultivated by the cultivation method is strong and can grow fast; the content of cordycepin in the mycelium obtained by fermentation is high; fermentation liquid and the mycelium which are cultivated by the method can be used for producing health-care caterpillar fungus products.

Description

technical field [0001] The invention relates to the field of microorganism culture and fermentation, in particular to a method for cultivating Cordyceps sinensis by using ginseng extracts and its application. Background technique [0002] Cordyceps sinensis is a precious and rare traditional Chinese medicine in my country. It has a long history of medication. It is one of the three major tonics together with ginseng and velvet antler. Cardiac insufficiency, etc., have anti-tumor, immune-enhancing, anti-virus, anti-fungal and other pharmacological effects. Scientific research has found that Cordyceps sinensis contains adenosine, cordycepin, cordycepic acid, polysaccharides and other pharmaceutical chemicals, which are the effects of Cordyceps sinensis. The important material basis for formulating the quality standards of Cordyceps sinensis. In recent years, scientists at home and abroad have discovered that cordycepin can interfere with the synthesis of gene fine RNA and DNA, i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12G3/04A23L33/135C12R1/645C12R1/79
CPCA23V2002/00C12G3/04C12N1/14A23V2200/30
Inventor 陈家任陶黎明童佳琼
Owner 锬酃藏虫草生物科技(深圳)有限公司
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