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Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof

A technology of Cordyceps sinensis and Astragalus, applied in the fields of fermentation and microbial culture, can solve the problems such as no reports of astragalus regulating microbial metabolism, no reports of Cordyceps Astragalus producing higher active components, etc., and achieve the effects of good quality and fast growth.

Inactive Publication Date: 2017-02-22
锬酃藏虫草生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report that Astragalus is used to regulate microbial metabolism, and there is no report that Astragalus is used to promote the production of higher active ingredients by Cordyceps fungus

Method used

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  • Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof
  • Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of astragalus superfine powder:

[0048] Purchase Astragalus root produced in Shanxi from the medicinal materials market, remove the dust on the surface, put it in a blast electric drying oven, control the temperature at 55 degrees, dry for 10 hours, cut into 0.3cm small pieces, send it to a mechanical pulverizer for intermittent pulverization, and obtain 60 meshes The fine powder is fed into an ultrafine pulverizer for intermittent temperature-controlled pulverization, and the temperature of the pulverization process is controlled to be less than 65 degrees to obtain astragalus superfine powder with a particle size of 300 mesh. The superfine powder of astragalus can be added as an accelerator to the cultivation and / or fermentation process of Cordyceps sinensis seeds.

[0049] (2) Preparation of Astragalus extract:

[0050] Take 100 grams of the superfine powder of Astragalus membranaceus, put it in an ultrasonic extractor, add 800 ml of pure water, con...

Embodiment 2

[0055] The strain of Cordyceps sinensis is isolated and purified from natural Cordyceps sinensis in Nagchu, Tibet, and identified as Cordyceps sinensis by molecular biology such as strain culture characteristics, microscopic characteristics, and rRNA gene sequence.

[0056] Cultivate and ferment according to the following steps:

[0057] (1) the Cordyceps sinensis bacterial classification is inserted into the liquid seed culture medium, and the seed liquid is cultivated according to a conventional method;

[0058] The formula of described liquid seed medium is: potato 200g / L, glucose 16g / L, peptone 3g / L, yeast extract 1.5g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.3g / L, distilled water surplus quantity;

[0059] In the case of carrying out seed parallel culture, add 4g / L astragalus superfine powder to the liquid seed culture medium during the exponential growth phase of the seed culture in the test group, as a test group; without adding astragalus superfin...

Embodiment 3

[0070] Compared with Example 2, the difference is only: in the case of carrying out seed parallel cultivation, the test group added 6g / L astragalus superfine powder to the liquid seed medium in the early stage of the seed medium without inserting the seeds, as The test group; the control group without superfine powder of astragalus.

[0071] After the fermentation was finished, the Cordyceps sinensis fermentation fluids of the test group and the control group were respectively taken. Add methanol at a ratio of 1:1.2 by volume, and ultrasonically treat it for 30 minutes under the conditions of power 400W and frequency 30KHz, and the extract is determined by the high-performance liquid chromatography as described for adenosine and cordycepin in the fermentation broth of the test group and the control group content, and the test results are shown in Table 2.

[0072] Table 2: Contents of adenosine and cordycepin per 100ml of fermentation broth

[0073] Adenosine cont...

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Abstract

The invention relates to a method for culturing cordyceps sinensis by utilizing astragalus membranaceus and an application thereof. The method comprises the following steps of: in the culture and / or fermentation process of cordyceps sinensis seeds, adding the astragalus membranaceus into a culture medium of the cordyceps sinensis, and continuously culturing and fermenting according to the conventional process of the cordyceps sinensis, wherein the preferred cordyceps sinensis is anamorph Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tolypocladium sinense, synnema or paecilomyces hepiali. The method provided by the invention is simple and convenient, the needed astragalus membranaceus extract is less and the cost is low; and the strain is fast in growth and excellent in quality, and the obtained fermentation liquid and mycelia are high in cordycepin content. The fermentation liquid and mycelia cultured by the method can be used for producing cordyceps sinensis health-care products.

Description

technical field [0001] The invention relates to the field of microorganism culture and fermentation, in particular to a method for cultivating Cordyceps sinensis by using Astragalus membranaceus and its application. Background technique [0002] Cordyceps sinensis is a precious and rare traditional Chinese medicine in my country. It is a complex of ergot fungi parasitizing on the larvae and larvae formed by bat moth larvae. It is mainly produced in Tibet, Qinghai, Sichuan, Yunnan and other provinces in Qinghai-Tibet alpine regions. Cordyceps sinensis It has a long history of medication, and it is one of the three major tonics together with ginseng and deer antler. It is used to replenish lung and kidney deficiency, chronic cough, bronchitis, impotence, irregular menstruation, hyperlipidemia, and insufficient blood supply to the heart. It has anti-tumor, enhancing Immunity, anti-virus, anti-fungal and other pharmacological effects, scientific research has found that Cordyceps s...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12G3/04A23L33/135C12R1/645C12R1/79
CPCA23V2002/00C12G3/04C12N1/14A23V2200/30
Inventor 陈家任陶黎明童佳琼
Owner 锬酃藏虫草生物科技(深圳)有限公司
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