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Paecilomyces hepiali producing coremia on silkworm pupae and culture method thereof

A bat moth Paecilomyces spp. and sporophore bundle technology, which is applied in the field of microorganisms and achieves the effect of good application prospect and low cost

Active Publication Date: 2015-11-18
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, so far, the production of Paecilomyces hematalis is limited to liquid fermented mycelium
The Paecilomyces bat moth strain is derived from natural Cordyceps sinensis specimens, and it will be closer to its natural state when it is inoculated on insects, and for fungi, the active ingredients and pharmacological effects of artificially cultivated fruiting bodies or spore bundles are both Different from liquid fermentation powder, but there is no report of Paecilomyces hematalis strains cultured on silkworm chrysalis to form fruiting bodies or spore bundles

Method used

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  • Paecilomyces hepiali producing coremia on silkworm pupae and culture method thereof
  • Paecilomyces hepiali producing coremia on silkworm pupae and culture method thereof
  • Paecilomyces hepiali producing coremia on silkworm pupae and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Acquisition and Identification of Paecilomyceshepiali DCH-60

[0042] The bacterial strain DCH-60 of the present invention is isolated from natural Cordyceps sinensis specimens (the Cordyceps sinensis specimens were collected from the Yushu area of ​​Qinghai in 2013), through the observation of the morphological characteristics of its colony, hyphae and conidia, combined with nuclear rDNA-ITS sequencing, the Strains were classified and identified.

[0043] 1. Morphological features

[0044] The strain DCH-60 was cultured on potato dextrose agar medium (PDA) at 25°C for 2 weeks. The diameter of the colony was about 5cm. figure 1 and figure 2 . Hyphae septated, transparent, 1.2-4μm in diameter; conidiophores are directly born on aerial mycelia, solitary, opposite or whorled, (5.0-23)×(1.5-3.0)μm; conidia Single cell, transparent, oval, (2.0-3.0)×(1.2-2.0)μm.

[0045] 2. Molecular biological characteristics

[0046] The rDNA-ITS sequence is used as a barcod...

Embodiment 2

[0050] Embodiment 2, cultivate bat moth Paecilomyces (Paecilomyceshepiali) DCH-60 on silkworm chrysalis to produce spore bundles

[0051] Paecilomyces spp. of the present invention is cultivated on silkworm chrysalis to produce spore bundles, including the steps of strain activation, preparation of liquid strains, selection of silkworm chrysalis, inoculation of silkworm chrysalis, culture of stiff pupae, cultivation of spore bundles and harvesting and drying, specifically as follows :

[0052] (1) Strain activation: the Paecilomyceshepiali DCH-60 strain preserved on the slant was transferred to a potato dextrose agar plate for activation (2 weeks of static culture at 20°C).

[0053] Wherein, the potato dextrose agar medium is prepared according to a method comprising the following steps: 200 g of potato juice is filtered, 20 g of glucose is added, and the volume is adjusted to 1000 ml; the pH is natural.

[0054] (2) Preparation of liquid strains: Inoculate the activated stra...

Embodiment 3

[0063] Embodiment 3, the efficacy analysis of the spore bundle of Paecilomyceshepiali (Paecilomyceshepiali) DCH-60

[0064] The adenosine content of the spore bundle of Paecilomyceshepiali DCH-60 obtained in Example 2 was determined by high performance liquid chromatography (HPLC). details as follows:

[0065] Adenosine standard product: product of Sigma Company, CAS No. 58-61-7.

[0066] Standard curve determination: Accurately weigh 10 mg of adenosine standard substance, dissolve it in mobile phase (recipe below) to a constant volume of 100 ml, shake well, store in refrigerator at 4°C, valid for 1 month. Accurately draw 0.1, 0.2, 0.5, 1, 2 and 5ml of the adenosine standard solution prepared above into a 10ml volumetric flask, dilute to volume with water and shake well, the concentration of the standard curve is 1, 2, 5, 10, 20 and 50 μg / ml, determined according to the chromatographic conditions of the sample (see below), with the adenosine concentration as the abscissa (X)...

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Abstract

The invention discloses paecilomyces hepiali producing coremia on silkworm pupae and a culture method thereof. The paecilomyces hepiali is particular paecilomyces hepiali DCH-60, the preservation number in the China General Microbiological Culture Collection Center is CGMCC No.10901. According to the paecilomyces hepiali producing the coremia on the silkworm pupae and the culture method thereof, the paecilomyces hepiali DCH-60 is obtained by being separated from natural cordyceps sinensis, culture is conducted on the silkworm pupae, and the coremia are obtained, and the paecilomyces hepiali producing the coremia on the silkworm pupae is expected to serve as health-care food or medicine to be developed.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Paecilomyces spp. which produces spore bundles on silkworm chrysalis and a cultivation method thereof. Background technique [0002] Cordyceps sinensis [Ophiocordycepssinensis (Berk.) G.H.Sung, J.M.Sung, Hywel-Jones & Spatafora (≡Cordycepssinensis (Berk.) Sacc.)] is a traditional precious medicinal material and tonic in my country, but its natural production is very limited, so it is necessary to look for its substitutes to satisfy People's health needs and the relief of human needs are of great significance to the pressure on natural resources. [0003] Paecilomyceshepiali Q.T.Chen&R.Q.DaiexR.Q.Dai,X.M.Li,A.J.Shao,ShuF.Lin,J.L.Lan,WeiH.Chen&C.Y.Shen) Dai Ruqin, Institute of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine In 1989, a new species of Paecilomyces was isolated from Cordyceps sinensis collected in Diqing, Yunnan. The main components ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/40C12R1/79
Inventor 董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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