366 results about "Penicillium bilaiae" patented technology

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

A microbial leaven used for preparing organic or microbial fertilizer from organic waste is prepared from at least two of nine microbes. Aspergillus, Sporotrichun, Mucoraceae, Penicillium sp, Ceotrichum sp., Neurospora, Bacillus, Saccharomyces and Streptomyces sp.

The invention discloses a compound bacterium for preventing and treating root-knot nematode and a preparation method thereof. The compound bacterium for preventing and treating root-knot nematode is prepared by the following steps: carrying out high-density cultivation on raw material strains of bacillus thuringiensis, pseudomonas fluorescens, bacillus subtilis, streptomycete, aspergillus niger, paecilomyces lilacinus and fusarium oxysporum, inoculating activated and cultivated strains in a fermentation tank and implementing fermentation and extended cultivation; dehydrating and drying various single strains obtained from extended cultivation to obtain resting microorganism dry powder; and mixing 10-20 parts of bacillus thuringiensis, 5-10 parts of pseudomonas fluorescens, 10-20 parts of bacillus subtilis, 10-20 parts of streptomycete, 10-15 parts of aspergillus niger, 10-25 parts of paecilomyces lilacinus and 8-15 parts of fusarium oxysporum to obtain the compound bacterium for preventing and treating root-knot nematode. The compound bacterium disclosed by the invention is used for preventing and treating root-knot nematode; and compared with an existing product, the compound bacterium is environment-friendly, harmless and non-toxic to a human body and good in control efficiency.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

An artificial culture method of new balm cricket Penicillium notatum and extraction and application of delivery pains compounds are disclosed. This invention involves declutching and breeding quality new balm cricket Penicillium notatum APC-20 from nature, researching production of the strain, further, defining method of extracting delivery pains compounds to be medicine. The compound is N6-(2- hydroxyethyl) adenosine. Molecular weight is 311.297. Molecular formula is C12H17N5O5. Delivery pains ratio is 99%by the method of mouse test. Principle of delivery pains is particular and cannot induce addiction.

The invention discloses a new method for controlling vegetable root knot nematode, which is characterized in that one or two from chitin, aropaz, Paecilomyces lilacinus and nematicidal pioneer is/are selected to process a seedbed in 1 to 2 weeks before the field planting of vegetables; 1.0Kg to 2.0Kg /mu (1 acre equals to 6.07 mu) of Fosthiazate and 20Kg to 60Kg/mu of harmonia mundi are mixed to process soil before the field planting; after the field planting, a biological agent is matched and used; the method is convenient to be used, is safe and effective, not only can effectively control the root knot nematode, but also can promote the growing of the vegetable plants, has the double effects of pesticide and fertilizer and can lighten the environment pollution brought by the use of the pesticide in large dosage; in the early period, the numbers of the eelworms in the soil are reduced by the mechanical eelworm pesticide of Fosthiazate; the stress resistance of the vegetables are increased by the chitin and the pesticide-fertilizer harmonia mundi; during the later period of the field planting, the numbers of the eelworms remained in the soil are controlled by using any of the biological agent of chitin, the aropaz, the Paecilomyces lilacinus and the nematicidal pioneer to irrigate the root, thus achieving the effect of controlling the vegetable root knot nematode by the cooperation of biology and chemistry and having very high popularization value.

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

The invention provides compound microbial fertilizer with disease and pest resistance. The compound microbial fertilizer comprises raw materials in parts by weight as follows: 5-10 parts of a compound microbial agent, 50-76 parts of yeast powder, 12-20 parts of an auxiliary material, 10-18 parts of waste molasses and 1-3 parts of paecilomyces lilacinus, wherein the compound microbial agent comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and bacillus licheniformis. A preparation method comprises following steps: the yeast powder and the auxiliary material are mixed, sieved and sterilized for preparation of organic powder; then the organic powder and paecilomyces lilacinus are mixed sufficiently for granulation, and organic granules containing paecilomyces lilacinus are prepared; the prepared compound microbial agent and the waste molasses are mixed, and a thick liquid is prepared and sprayed to the surfaces of the organic granules. A multi-level antagonism isolating mode is adopted, so that the components not only can resist diseases and kill insects, but also can promote mutually and produce a synergistic effect for promotion of plant growth and increase of crop yield. The invention further provides an application of the compound microbial fertilizer with disease and pest resistance in crop growth.

The invention discloses a method for improving the yield and product activity of a liquid fermentation product of Paecilomyces cicadae, which comprises the following steps: (1) extracting traditional Chinese medicinal materials composed of 10-40 parts by weight of coix seed, 10-40 parts by weight of radix astragali, 10-40 parts by weight of Chinese wolfberry and 10-40 parts by weight of rhizoma atractylodis macrocephalae through a water solution, thus obtaining a traditional Chinese medicine compound water extract liquid; (2) adding the traditional Chinese medicine compound water extract liquid prepared in the step (1), rhizoma atractylodis macrocephalae lactone and Tween-80 into a fermentation basal culture medium, thus obtaining a Paecilomyces cicadae fermentation culture medium; and (3) inoculating a Paecilomyces cicadae strain into the Paecilomyces cicadae fermentation culture medium prepared in the step (2), performing liquid fermentation to obtain a fermentation liquid, and separating to obtain a fermentation product. According to the invention, particular kinds of traditional Chinese medicine compound water extract liquid, rhizoma atractylodis macrocephalae lactone and Tween-80 are added, so that the biomass and polysaccharide yield of the Paecilomyces cicadae can be obviously improved, and meanwhile, the activity of the polysaccharide is also improved obviously.

The invention relates to a method for producing a preparation with biological bacteria, which is used for preventing plant nematodes and the application of the preparation, in particular to a paecilomyces cicadae biological bacteria preparation, a method for producing the same and the application of the same to plant nematode prevention. The paecilomyces cicadae biological bacteria preparation can be the compound of paecilomyces cicadae, culture medium of the paecilomyces cicadae, auxiliary materials and a carrier and is produced by culturing paecilomyces cicadae, fermenting the paecilomyces cicadae with liquid, propagating the paecilomyces cicadae in great qualities, directly processing the paecilomyces cicadae and absorbing the paecilomyces cicadae with the auxiliary materials and the carrier. The paecilomyces cicadae biological bacteria preparation has obvious effect on plant nematode prevention, stable performance and wide application range, reaches the standard of the quality guarantee period of biological pesticide, meets the production requirements of organic food and green food, is environmental friendly, beneficial to human health and prone to large-scale industrial production and serves as broad-spectrum biological bacteria preparation for preventing plant nematodes.

The invention provides a processing method of pu-er tea with inoculated fungi microorganism. During the first day of pu-er tea pile-fermentation, aspergillus niger red koji and penicillum sp. red koji is mixed together by the ratio of 1-2:1 and is added into the production process of pu-er tea by the weight percentage of uniform mixed red koji and basic tea of 1:125-250; during the second day of pu-er tea pile-fermentation, saccharomyces sp. is added into the production process of pu-er tea by the weight percentage of saccharomyces sp. and basic tea of 1:250-500 and then material is gathered together and covered with fermentation cloth; fermentation gathering temperature is controlled between 40 celsius degree and 65 celsius degree and gathering overturning has to be carried on apporiately to control moisture content between 14 percent and 20 percent; when tea leaves moisture content is over than 20 percent, ditch recanalization has to be carried on every day; when tea leaves moisture content is 14 percent to 20 percent, ditch recanalization has to be carried on every three days; with shortened processing time, rich water extract, proper tea polyphenol and amino acids is retained and a certain amount of caffeine, hydration pectin, theaflavins and theabrownin is added; compared with natural pile-fermentation, the quality of the invention is superior and more stable and microbial indicator is equal to that of red tea and green tea.

The invention discloses a paecilomyces fumosoroseus strain. The strain is a wild strain separated from bemisia tabaci body in Guangzhou area, which is naturally infected by worm living fungus. The wild strain is re-planted on the bemisia tabaci body for the rejuvenation, so that the paecilomyces fumosoroseus strain is obtained; the purified strain is obtained through single spore isolation. The paecilomyces fumosoroseus strain is PF01-N10; the preservation number of the strain is CCTCC No: M207088; the strain is stored in China Center for Type Culture Collection on 27 June, 2007. Through a long term of infectious biology research and the indoor biology testing, the paecilomyces fumosoroseus has strong infectious and killing effects for a plurality of sucking mouth parts insects, such as the white fly and the cotton worm etc, and the chewing mouth parts.

The invention discloses a ghost moth paecilomyces leavening feed additive. Ghost moth paecilomyces leavening is obtained in such a way that myceliums of a high-yield bacterial strain obtained by mutagenizing ghost moth paecilomyces separated from worm grass by ion beams are dried and crushed together with a a culture medium after strain preparation, shaking culture, primary starter can fermentation, fermentation can fermentation, solid culture fermentation. The invention also discloses an application of the ghost moth paecilomyces leavening feed additive in the preparation of animal feed, and the ghost moth paecilomyces leavening feed additive is added in the animal feed by 0.05 to 0.5 percent . The ghost moth paecilomyces leavening feed additive can increase the daily gain of animals, obviously promote the growth of the animals, improve the conversion rate of the feed, enhance the production performance and the antiviral capability of the animals and can also improve the quality of livestock and fowl meat. The ghost moth paecilomyces leavening has simple preparation technology flows, simple-formula culture medium and cheap and easily obtained raw materials.

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

The invention discloses a penicillium deumbens engineered strain containing over-expressed beta-glucosidase, which contains over-expressed beta-glucosidase genes, is named penicillium deumbens Gi31-2 and is preserved in China Center for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010111. The invention also discloses the application of the strain in production of the beta-glucosidase and degradation of cellulose. The highest activity of the beta-glucosidase of the engineered strain of the invention reaches 3.47 IU/ml and is 2.90 times as high as that of an original host strain, while highest filter paper activity is 3.32 IU/ml and is 1.23 times as high as that of the original host strain. Therefore, the strain is widely applied to the production of the beta-glucosidase.

The invention discloses Penicillium sp.ECU0913 capable of degrading biomass and with preservation number of CGMCC No.3371 and application thereof. By using a Penicillium strain, cellulose and xylanase can be obtained by simultaneous fermentation, the biocatalysis efficacy can be exerted, biomass rich in cellulose and hemicellulose can be decomposed and monosaccharide can be generated; and the obtained hydrolysate which can be used as a carbon source is used for synthesizing a biological energy source and bio-based chemical products.

The present invention provides a microbial fertilizer for preventing and controlling citrus Huanglongbing, and a preparation method thereof. The microbial fertilizer comprises Paecilomyces lilacinus, Bacillus lincheniformis, Trichoderma and organic matter, wherein the viable organism content of the Paecilomyces lilacinus is not less than 20 million/g, the viable organism content of the Bacillus lincheniformis is not less than 10 million/g, and the viable organism content of the Trichoderma is not less than 5 million/g. According to the present invention, the three strains such as the Paecilomyces lilacinus, the Bacillus lincheniformis and the Trichoderma co-act to inhibit or damage the development process of nematode eggs so as to reduce the nematode adult rate, reduce the basis occurrence amount of orchard nematode, cut the Huanglongbing media and achieve the citrus Huanglongbing prevention and control purpose. The present invention further provides a preparation method of the microbial fertilizer.

The invention discloses a bioorganic fertilizer for adsorbing heavy metals and improving greenhouse soil. Raw materials of the bioorganic fertilizer comprise, by weight, 83-88 parts of a decomposed organic fertilizer, 8-12 parts of biochar, 1-2 parts of attapulgite, 2-3 parts of functional bacteria, and 0.5-1 part of methyl salicylate, wherein raw materials of the decomposed organic fertilizer comprise, by weight, 45-60 parts of straws, 30-45 parts of traditional Chinese medicinal residues, 1-5 parts of a urea fertilizer with the nitrogen content of 46wt%, 1-2 parts of a straw fermentation inoculant, 1-2 parts of penicillium oxalicum and 0.5-1 part of brown sugar. The content of organic matters in the bioorganic fertilizer is not lower than 30%, the quantity of effective live bacteria in the bioorganic fertilizer is not lower than 0.02 billions/g, and the bioorganic fertilizer can effectively enrich heavy metals in soil and reduces the content of the heavy metals; and functional bacteria and methyl salicylate can dually defense internally and externally, can effectively inhibit breeding and invasion of root-knot nematodes, also can improve the structure of soil, increases the permeability, improves the pH value, and reaches yield increase, quality improvement and environmental protection.

The invention provides a preparation method of composite biological organic fertilizer granules. The fertilizer granules comprise the following effective components: 50 to 55 parts of bacillus subtilis, 10 to 15 parts of actinomycetes, 8 to 10 parts of photosynthetic bacteria, 6 to 10 parts of paecilomyces lilacinus, 3 to 5 parts of saccharomycetes and 2 to 4 parts of bifidobacteria. The fertilizer granules which are prepared in a compositing manner have complete functions and high complementarity and can be mixed with chemical fertilizers and organic fertilizers, thereby improving the utilization rate of the fertilizer granules by 30% or so. The fertilizer granules are used for resisting diseases and inhibiting bacteria, improving soil fertility, activating soil, increasing yield and improving quality, resisting the lodging and decreasing pesticide residues, and are long in storage life, wherein the flower retention rate is improved by 50%; the fruit-bearing is advanced by 7 to 10 days; and the yield is increased by 15% to 20%. In addition, the fertilizer granules contain the white spines actinomycete streptomyces, thereby having obvious effects for inhibiting the pathogenic fungi of the fusarium wilt of cucumbers from growing. The experimental result proves that the morbidity of the fusarium wilt of the cucumbers is below 10% after the fertilizer granules provided by the invention are applied to the soil in which the fusarium wilt of the cucumbers occurs.

The invention relates to an enzyme liquid treatment for agricultural waste composts, and provides a fast-rotting reactive additive which is prepared by mixing bacillus, aspergillus niger, trichoderma and blue mould proportionally for fermentation, producing composite enzyme liquid, filtering the liquid state fermentation liquid. The liquid can be applied directly by 0.5-1 wt% of the compost raw material at the beginning of the composts preparation. The prepared reactive additive contains a large quantity of pectin enzyme and ligno-cellulose. The addictive can facilitate the degradation of pectin, hemicellulose, cellulose in one fermentation stage, destruct vegetable cell wall tissue, thus quickening the compost rotting course.