Liquid fermentation culture method of Paecilomyces lilacinus

A liquid fermentation technology of Paecilomyces lilacinus, applied in the field of liquid fermentation culture of fungi, can solve the problems of decreased biocontrol effect, immaturity, strain variation, etc., and achieve the effect of low production cost and high spore yield

Inactive Publication Date: 2011-12-21
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing Paecilomyces lilacinus fermented culture and research on fermentation technology are still very immature, and there are many problems that need to be solved urgently, for example, along with the composition of culture med...

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  • Liquid fermentation culture method of Paecilomyces lilacinus
  • Liquid fermentation culture method of Paecilomyces lilacinus
  • Liquid fermentation culture method of Paecilomyces lilacinus

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experiment example

[0025] EXPERIMENTAL EXAMPLE Optimum Screening Experiment of Various Process Parameters of Paecilomyces lilacinus Liquid Fermentation

[0026] 1. Experimental method

[0027] 1.1 Options for basal medium

[0028] 1.1.1 Strains

[0029] The strain of Paecilomyces lilacinus (P.lilacinus) was purchased from China Forestry Microorganism Culture Collection and Management Center, and the strain number is cfcc 86080.

[0030] 1.1.2 Materials

[0031] C source: glucose, sucrose, maltose, soluble starch, corn flour;

[0032] N source: sodium nitrate, ammonium sulfate, peptone, soybean powder;

[0033] Inorganic salts: potassium dihydrogen phosphate, magnesium sulfate;

[0034] 1.1.3 Method

[0035] Culture medium combination: The above carbon sources and nitrogen sources are fully arranged and combined to form 20 culture formulas (see Table 1).

[0036] Medium ratio (g / L, g / L): carbon source 40, nitrogen source 20, inorganic salt 0.5.

[0037] Strain preparation: Take 15 mL of T...

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Abstract

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

Description

technical field [0001] The invention relates to a method for liquid fermentation and cultivation of fungi, in particular to a method for liquid fermentation and cultivation of Paecilomyces lilacinus (P. lilacinus), and belongs to the field of liquid fermentation and cultivation of Paecilomyces lilacinus. Background technique [0002] Paecilomyces lilacinus (P.lilacinus) belongs to the class Deuteromycetes, Hyphomycetes, Hyphosporaceae, and Paecilomyces genus. It is widely distributed all over the world and has the advantages of high efficacy, wide host range, and easy cultivation. Paecilomyces lilacinus is currently one of the main natural enemy fungi for the control of plant parasitic nematodes, and it is of great research and development value. Paecilomyces lilacinus is an effective parasite for the eggs of root-knot nematode incognita and white cyst nematode, and the parasitic rate of eggs of root-knot nematode incognita is as high as 60%-70%. It has control effect on a ...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12R1/79
Inventor 汪来发朴春根王曦茁李永刘春秀郭民伟田国忠林彩丽
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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