A tissue cultured seedling micrografting method for acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of cutting an acer elegantulum branch segment with sprouts, performing tissue culture to obtain an acer elegantulum sterile seedling, and cutting the acer elegantulum sterile seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of cutting an acer palmatum 'orange dream' branch segment with sprouts, performing tissue culture to obtain an acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a greenhouse, performing earlier-period seedling hardening, transferring into a seed bed, performing seedling hardening, performing mist spray treatment, sterilizing, and transplanting after the seedling hardening is completed. The method increases the grafting efficiency, increases the reproductive output, is high in survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.