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A tissue cultured seedling micrografting method for acer palmatum 'orange dream'

A technology of micro-grafting and tissue culture seedlings, which is applied in grafting, horticultural methods, botanical equipment and methods, etc., can solve the problems of short cultivation period of grafted seedlings, small number of reproduction, high labor costs, etc., and solve the problem of difficult rooting of tissue culture , simple formula and short cultivation period

Active Publication Date: 2016-04-27
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the application provides a method for micro-grafting of Japanese maple orange dream tissue culture seedlings, said method can improve the efficiency of grafting, increase the number of reproduction, controllable environment, high survival rate, short grafting seedling cultivation cycle, can Not limited by seasons, it overcomes the shortcomings of traditional grafting methods that are limited by seasons, the number of reproduction is small, the survival rate is low, and the labor cost is high. Large-scale industrial seedling cultivation and deep processing can be carried out

Method used

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  • A tissue cultured seedling micrografting method for acer palmatum 'orange dream'
  • A tissue cultured seedling micrografting method for acer palmatum 'orange dream'

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Embodiment 1

[0027] The micro-grafting method of the tissue culture seedling of Japanese maple orange dream described in the present embodiment may further comprise the steps:

[0028] (1) Cultivation of sterile rootstocks: Cut out the section of Acer cerevisiae with buds, inoculate it on the axillary bud induction medium after disinfection and sterilization; cut off the germinated axillary buds, inoculate them on the cluster bud induction medium for cultivation; The clustered buds are cut into individual plants, inoculated with rooting medium and cultivated to obtain aseptic seedlings of Acer schullii, which are obliquely cut and cut off to obtain sterile rootstocks with a stem thickness of more than 1.5 mm;

[0029] (2) Cultivation of sterile scions: cut out the Japanese maple orange dream band bud branch, inoculate it on the axillary bud induction medium after disinfection and sterilization; The differentiated cluster buds were cut into individual plants, inoculated with rooting medium ...

Embodiment 2

[0033] The micro-grafting method of the tissue-cultured seedlings of the dream of the Japanese maple orange described in the present embodiment differs from Example 1 in that:

[0034] Step (3) In the micro-grafting of tissue-cultured seedlings, the composition of the medium is: MS basic medium supplemented with 1.0 mg / L 6-BA, 0.05 mg / L NAA, 20 g / L sucrose, and 4 g / L agar.

Embodiment 3

[0036] The micro-grafting method of the tissue-cultured seedlings of the dream of the Japanese maple orange described in the present embodiment differs from Example 1 in that:

[0037] Step (3) In the micro-grafting of tissue-cultured seedlings, the composition of the medium is: MS basic medium supplemented with 0.5mg / L6-BA, 0.03mg / LNAA, 10g / L sucrose, and 4g / L agar.

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Abstract

A tissue cultured seedling micrografting method for acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of cutting an acer elegantulum branch segment with sprouts, performing tissue culture to obtain an acer elegantulum sterile seedling, and cutting the acer elegantulum sterile seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of cutting an acer palmatum 'orange dream' branch segment with sprouts, performing tissue culture to obtain an acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a greenhouse, performing earlier-period seedling hardening, transferring into a seed bed, performing seedling hardening, performing mist spray treatment, sterilizing, and transplanting after the seedling hardening is completed. The method increases the grafting efficiency, increases the reproductive output, is high in survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.

Description

technical field [0001] The invention relates to the technical field of grafting and reproduction, in particular to a method for micro-grafting of tissue-cultured seedlings of Japanese maple orange. Background technique [0002] Japanese Maple Orange Dream (Acerpalmatum "OrangeDream") has high ornamental value and is a unique variety. In spring, the leaves are yellowish-green, and the more exposed to sunlight, the leaf color becomes yellower. It turns into a very beautiful orange, with yellow veins and golden yellow in autumn. The leaf color changes obviously in the three seasons, and there is no sunburn phenomenon under the hot sun. It is very popular in the market. [0003] At present, Japan's Dream of Maple Orange mainly adopts the traditional grafting method, which is limited by the season, has a small number of reproductions, a low survival rate, and high labor costs. The minimum size of the rootstock is about 1 cm, which cannot produce small-sized grafted seedlings and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
CPCA01G2/30A01H4/008
Inventor 郑超吴佳川王小辉朱晓菲何程相
Owner SICHUAN COLORLINK CO LTD
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