263results about How to "Short cultivation period" patented technology

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

The invention relates to a propagation method for using a single-bud short tamarix to cut containerized seedlings. The propagation method comprises the main operating steps of: firstly establishing pergolas, preparing cutting containers, cutting matrixes and cutting beds of the containers, then carrying out cutting propagation operation, selecting the branches of the middle-upper part of the periphery of the middle-old age excellent clonal crown as cuttings, carrying out cutting in summer or autumn, doing well in work of weeding, fertilizing, moisturizing and prevention of pests. The propagation method has short culturing period, generally needs only 10 to 12 months, has simple operation, high propagation coefficients, fast propagation expansion and good transplanting effect, also can effectively reduce the loss of cutting propagation materials, and improve comprehensive benefit of industrial development of good seeds of the oil tea.

The invention discloses a fast culture method for camellia oleifera light substrate container cuttage seedlings, which mainly comprises the following steps of: 1, light substrate cuttage: selecting an open-air cuttage bed, using peat, fungus dreg and coconut tree branny as substrate raw materials, shearing semi-lignified ears into 2cm to 3cm single-leaf and single-bud cutting ears, soaking the ears by indol-3-butanoic acid and flushing the indol-3-butanoic acid solution on the blades after the cuttage; 2, management after the cuttage: carrying out moisture regulation and control until the root growth and adopting carbendazim 1000-time liquid for carrying out spraying prevention and treatment every eight days; and 3, management after root growth: carrying out seedling adaptation in 15 daysafter the root growth, adopting 6-benzylaminopurine to carry out coating treatment on buds of unsprouted ears after the root growth for a month and carrying out fertilizer and water management and pest control. The fast culture method has the advantages that the culture method has low cost and short culture period, the problems of unstable survival rate, insufficient root system development and the like caused by climate differences can be perfectly solved, the culture material loss can also be effectively reduced, and the economic value of the camellia oleifera fine breed resource development is improved.

The invention discloses a breeding method for a Puan black goat line. The breeding method utilizes foundation Guizhou black nanny goats and Nubia billy goats to bi-cross, first-class first Nubia billy goat-black nanny goat hybrid generation nanny goats chosen as the female parent and first-class Guizhou black billy goats chosen as the male parent backcross, first-class backcrossed filial generation billy goats and first-class nanny goats are chosen to cross to be fixed, and therefore the Puan black goat line with more stable genes is bred. By introducing the excellent genes of the Nubia goats, such as big build, high reproduction rate and fast growth, the breeding method ensures that the bred Puan black goat line has the advantages of big build, fast early growth and development, high reproduction rate, high meat productivity and the like besides the characteristics, such as high adaptability, high disease resistance and black clothing hair, of the Guizhou black goats.

The invention relates to a method for breeding plum blossom in the true mume group by grafting on an apricot tree, which has the advantages that the breeding cycle of plum blossom in the 'true mume group' is shortened, economic benefit is increased, and survival rate is high. The method comprises firstly, primary grafting, and secondly, secondary grafting. The primary grafting includes: selectingan apricot tree, collecting mume branches belonging to the 'Blireiana group' as scions, trimming and lopping branches of the apricot tree before grafting, watering the apricot tree once, restoring temperature of the scions to the outdoor temperature before grafting, lopping the scions into small segments, grafting the small segments of the scions to loppings of the branches of the apricot tree inspring, tightly binding the small segments to inosculate the scions with stocks in a layered manner, watering the apricot tree once again after grafting, and managing the apricot tree after grafting as common management after grafting. The secondary grating includes: the underyear branches of plum blossom belonging to the 'true mume group' as scions, using the scions belong to the 'Blireiana group' as inter stocks, and grafting the scions in the 'true mume group' to the inter stocks by the method of plate budding.

Disclosed are a ganoderma lucidum mycelium and a preparation method thereof. The ganoderma lucidum mycelium specifically comprises a culture medium composed of rice bran, wheat bran, corn flour and brown sugar. A ganoderma lucidum liquid strain is taken as a strain, an inoculation method and inoculation amount of the liquid strain are well grasped, and a high-quality ganoderma lucidum mycelium finished product is cultured by reasonable arrangement of the production environment. By implementation of the method, the problem that ganoderma lucidum fruiting bodies cannot be produced massively to further affect application of ganoderma lucidum medicine sources due to insufficient wild ganoderma lucidum resources and high artificial cultivation difficulty is solved. Meanwhile, the production and preparation technology of the final product, namely the ganoderma lucidum mycelium, has the advantages of short cultivation period, simplicity, low production cost, high production efficiency, high stability and the like. Besides, the final product, namely the ganoderma lucidum mycelium serving as an additive, is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like, and is quite good in market prospect.

The invention discloses a lucid ganoderma cultivation method. The method comprises the following steps of culture medium preparation, culture medium bagging and sterilization, lucid ganoderma protogenetic fungus cultivation, lucid ganoderma cultivation fungus cultivation, fungus stick inoculation, mycelium cultivation, lucid ganoderma growth management and timely harvesting. The lucid ganoderma cultivation method which is provided by the invention has the advantages that the fungus growing speed is rapid, the cultivation period is short, the cost is low, and the appearance of the cultured lucid ganoderma is attractive; in addition, the waste of tree resource is avoided by the lucid ganoderma cultivation method, and crop hulls, crop cores, straws and lucid ganoderma residues are fully utilized.

The invention relates to the technical field of culture of edible fungi, in particular to a glossy ganoderma culture method. The method comprises steps as follows: (1) preparation of a culture medium; (2) material stacking; (3) bagging and sterilization; (4) inoculation; (5) spawn running management; (6) fruiting management; (7) harvesting in due time. Fermented glossy ganoderma residues and walnut middle lamella shells are added, on one hand, nutrient substances can be absorbed by glossy ganoderma conveniently after fermentation of the glossy ganoderma residues, on the other hand, the fermented glossy ganoderma residues and the walnut middle lamella shells are matched for use, in cultured glossy ganoderma, the fruiting body bag weight is up to 39.38 g, the diameter of fruiting bodies is up to 12.8 cm, the polysaccharide content is up to 1.15%, and the total triterpenoid content is up to 0.18%; besides, no heavy metal ion is detected from the fruiting bodies. The glossy ganoderma culture method is high in spawn running speed, short in culture period and low in cost, and the shape of the cultured glossy ganoderma is attractive.

The invention belongs to the technical field of biology deep fermentation technology and particularly relates to a fast manufacture method for liquid strain. The method comprises the steps that for a culture medium, rice powder serves as a carbon source, sugar serves as an auxiliary carbon source, corn powder serves as a nitrogen source, peptone and yeast extract (powder) serve as auxiliary nitrogen sources, monopotassium phosphate and magnesium sulfate serve as inorganic salts, and water serves as a carrier; a glass container serves as a culture container; inoculation is performed after a liquid medium is sterilized, and the liquid strain is obtained through a rotary type shaking table. The fast manufacture method for the liquid strain has the advantages that cheap plant starch is used as the main carbon source and the nitrogen sources, and nutrition-allocated proportion is reasonable and comprehensive; the prepared liquid medium is appropriate in viscosity; a culture period is short, and the liquid strain with good quality, strong vitality and different sizes can be cultured in 2-3 days; at the time of inoculation, the fast manufacture method for the liquid strain is simple, convenient and fast; when a solid culture material is inoculated, the material flows fast and is easy to scatter, many strain running points appear and germinate fast and the like.

The invention belongs to the technical field of edible mushrooms and particularly relates to a hypsizygus marmoreus Finc-B-3 strain and application thereof. The hypsizygus marmoreus strain provided by the invention is named as Finc-B-3 and is obtained by taking a strain TNN-12 and a strain F-FG as parents to be hybridized through systematic selection, belongs to hypsizygus marmoreus and is preserved in Institute of Microbiology Chinese Academy of Sciences, wherein the address is No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.11204 and the preservation date is 20150813. Compared with the prior art, the hypsizygus marmoreus Finc-B-3 strain provided by the invention has short culture and cultivation periods, so that the production efficiency of factories is greatly improved; the unit yield is high, the yields of bottles are similar, and topographic characteristics are good, so that sub-packaging and sales of products are very easy; and the Finc-B-3 has good comprehensive performances and a wide market prospect.

The invention provides a hypsizigus marmoreus culture medium which comprises the following components in percentage by mass: 40-50% of corn cob, 8-15% of rice bran, 3-8% of schisandra fruit dreg, 10-20% of radix salviae miltiorrhizae dreg, 5-10% of soybean hull, 12-20% of wheat bran, 0.8-2% of shell powder and 0.2-1% of lime. The invention belongs to the technical field of edible fungus culture. The hypsizigus marmoreus culture medium provided by the invention does not need wood dust or cottonseed hull, fully utilizes the schisandra fruit dreg and radix salviae miltiorrhizae dreg, and thus, has the advantages of wide raw material sources, high air permeability, favorable culture effect and the like on the premise of lowering the cost.

The invention relates to a tomato soilless culture method, and belongs to the soilless culture technical field; the method comprises the following steps: matrix preparation, water soluble fertilizer preparation, greenhouse equipment configuration, wobble plate soil loading, spot planting and earthing, watering germination, colonization preparation, colonization, colonization management and harvesting; the matrix comprises coco coir, straw powder, turf, vermiculite, mushroom slag, wood chip, expanded perlite and iron sheet dendrobium; the water soluble fertilizer comprises a first nutrient solution and a second nutrient solution; the first nutrient solution comprises calcium nitrate, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, magnesium sulfate, EDDHA-PE-6, manganese sulfate, copper sulphate, ammonium molybdate, borax, zinc sulfate and water; the second nutrient solution refers to trace element nutrient solution; the tomato soilless culture method is green and environmental protection, can reasonably utilize planting space in a planting process, and the matrix and nutrient solution are rich in nutrition; the tomato grown by the method is nontoxic and pollution-free, and suitable for mass production and promotion.

The invention provides a method for preparing fleckedflesh polypore mycelium. The method specifically comprises the steps that a culture medium composed of rice bran, bran, corn flour, flour and brown sugar is provided, fleckedflesh polypore liquid spawn is used as spawn, the inoculation method and the inoculation amount of the liquid spawn are well grasped, and the fleckedflesh polypore mycelium is prepared through the reasonable arrangement of a production environment. By the adoption of the method for preparing the fleckedflesh polypore mycelium, the application problems that medicinal fleckedflesh polypore resources are influenced due to the fact that wide fleckedflesh polypore resources are insufficient and a large amount of fleckedflesh polypore cannot be produced due to the large difficulty of manual cultivation are solved. The method for preparing the fleckedflesh polypore mycelium has the advantages that the cultivation period is short, the production preparation technology is simple, the production cost of the product is low, and the production efficiency of the product is high and stable. In addition, the fleckedflesh polypore mycelium as the final product is used as an additive which is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like and is good in market prospect and remarkable in benefit.

The invention belongs to the technical field of edible mushrooms, and particularly relates to a white needle mushroom JK01 strain and application thereof. A needle mushroom provided by the invention is named JK01, is obtained by hybridizing a Taichangbai strain and a hybridized strain 19 serving as parents through systemic breeding, belongs to needle mushroom (Flammulina velutipes), and is collected in the Institute of Microbiology, Chinese Academy of Sciences (address: No. 3, 1# Yard, Beichen West Road, Chaoyang District, Beijing) with a collection number CGMCCNo.13877 on June 14, 2017. Compared with the prior art, the white needle mushroom JK01 strain provided by the invention has the advantages of short culturing period, consistent growth, high stress resistance, high yield, suitability for industrial cultivation, thick stems, superior shape characteristic, crispy mouthfeel, contribution to splitting and cooking of products, high comprehensive performance, and wide market prospect.

The invention relates to a hypsizigus marmoreus strain Finc-B-5, and a culture method, a planting method and application thereof. The hypsizigus marmoreus strain is named as Finc-B-5, belongs to hypsizigus marmoreus and is collected in General Microorganism Center of China Microbiological Culture Collection Management Committee, which is located at No.3, No.1 yard, Beichen west road, Chaoyang district, Beijing. The collection number is CGMCC No.11203, and the collection date is August 13 of 2015. Compared with the prior art, the hypsizigus marmoreus strain Finc-B-5 has the advantages that yield per unit is high, culture and planting periods are short, raw material conversion rate and factory production efficiency are improved greatly, energy is saved, and consumption is reduced; preservation period is long, long-distance transportation is facilitated, shelf life is prolonged, and significance in commercial popularization is great; the shape characteristics are excellent, and product subpackage and sales are really benefited; the mouthfeel is good, and customer acceptability is high; in conclusion, the Finc-B-5 has excellent overall performance and broad market prospect.

The invention discloses a method for cultivating Tibetan lucidum. The method comprises the steps of strain isolation, fungi bag preparation, lucidum cultivation, bud pressing management, lucidum cultivation and management, and harvesting. According to the method provided by the invention, the contents of macromolecular polysaccharide, triterpenes, adenosine, alkaloid and various enzymes can be enhanced, the in vivo environment can be effectively adjusted, the immunologic function can be enhanced, the metabolism can be promoted, blood purification can be realized, circulation can be improved, body function can be conditioned, the manual cultivation can be realized at the first time, the cultivation period is short, the yield is high, the quality is good, and the social and economic benefits are high.

The invention relates to a preparation method of a functional biological product, belongs to the technical field of biology and particularly relates to a method for preparing phellinus igniarius hypha blocks by utilizing sprouted rice. The method is characterized by comprising the following steps of: preparing a culture medium for production by utilizing most nutritive sprouted rice and a nutrient solution without inorganic salts; and reasonably controlling the inoculation method and inoculation amount of a phellinus igniarius liquid used as a strain, and reasonably arranging the production environment, so as to culture the natural, healthcare, environment-friendly and healthy phellinus igniarius hypha blocks. The phellinus igniarius hypha blocks are the unique combination of phellinus igniarius hypha and the sprouted rice, are more obvious in nutrient, function and efficacy, can be new favorite consumption of people due to beautiful shape, novelty, uniqueness, excellent taste and the like, and can be used for effectively solving the problem that the application of phellinus igniarius medicinal resource is influenced because phellinus igniarius sporocarps cannot be output with high yield due to insufficient wild phellinus igniarius resources and high difficulty in manual culture, and thus the value of the sprouted rice is greatly increased.

The invention discloses a factory culturing method for sweet potato seedlings with roots and tops. The method comprises the following steps of: establishing a culturing pool matrix, selecting seedlings, cutting seedlings, cutting, adding or pouring a nutrient fluid, and cutting the section seedling, and then the seedling with root and top is cultured. A support disc can be arranged on an agriculture film at the bottom of the culturing pool, the matrix is arranged on the surface of the support disc, and the nutrient fluid is arranged under the surface of the support disc. The nutrient liquid is prepared from the following components in percentage by weight: 48-52% of nitrate nitrogen three-element complex fertilizer, 25-30% of urea, 15-20% of potassium sulphate, 3-5% of potassium dihydrogen sulfate, and 1-3% of copper, zinc, manganese, magnesium, and molybdenum. The invention has the advantages that the culture period is short; the seeding has apical dominance and has roots including main root, side root and storing root; the root can continuously grow after being planted into fields, the drought resistance performance is strong, and the seedling return period does not exist; the growth in the field in the early period is strong, the sweet potato is early to form, the quantity of the formed sweet potatoes is much, and the yield of the sweet potatoes is high; the operating method is simple, and the integration cost is low.

The invention discloses a domestic fungus culture medium. The culture medium comprises, by weight, 15-20 parts of bean residues, 20-30 parts of rick husks, 15-25 parts of cassava stalk powder, 15-25 parts of spinach leaf powder, 10-15 parts of potatoes, 10-20 parts of corn flour, 5-10 parts of beef extract, 10-20 parts of guava leaves, 1-5 parts of vitamins, 2-8 parts of ammonium sulfate, 1-6 parts of gypsum, 1-3 parts of urea, 2-10 parts of Chinese yam powder, 2-10 parts of Spica Prunellae, 1-5 parts of iron citrate, 2-10 parts of silkworm chrysalis powder, 1-5 parts of licorice root and 1-6 parts of fermented chicken manure. The culture medium has the advantages of fast spawn running speed, short culture period, high output, low cost, simple composition, improvement of the output and the nutrition of edible fungi, enhancement of the disease resistance and the competitor infection resistance of the edible fungi, effective killing of competitors and viruses, guaranteeing of the healthy growth of the edible fungi, no pollution, no pesticide residual and low content of heavy metals, and is suitable for culturing Pleurotus eryngii, needle mushroom, Pleurotus citrinopileatus, Pleurotus ostreatus, Pleurotus nebrodensis, shiitake, edible tree fungus and various other edible fungi.

The invention discloses a biological organic fertilizer with disease preventing function and a preparation method thereof. The biological organic fertilizer with the disease preventing function contains thermophilic bacterium, actinomyces, streptomyces, adsorption carrier and fermentation carrier, wherein the weight ratio of the bacteria is 0.8-1.2:0.8-1.2:0.8-1.2, and the adsorption weight ratio of the mixed bacteria solution of the bacteria to the adsorption carrier is 1:1.25-1.75. The preparation method comprises the following steps of: performing seed culture and fermentation tank culture on the thermophilic bacterium, the actinomyces and the streptomyces respectively, mixing the bacteria according to a proportion, performing carrier adsorption to obtain a composite bactericide, and drying the composite bactericide at a low temperature till the water content is below 30 percent; and inoculating the obtained composite bactericide to the fermentation carrier to perform mixed fermentation, wherein the weight ratio of the composite bactericide to the fermentation carrier is 1: 3,000-1: 5,000. The biological organic fertilizer provided by the invention has the characteristics of broad pesticidal spectrum, short breeding cycle and disease preventing function.

The invention relates to a cultivation method of a leaflet privet stump, which comprises the following cultivation steps: excavating, treating soil and root systems, cutting bars, accumulating branches, picking buds, scratching, binding and modeling. The cultivation method for the leaflet privet stump has short cultivation period and low cost.

The invention discloses a Machilus pauhoi full-exposure cuttage fast propagation method. The method includes: selecting a full exposure cutting bed which is leeward, good in water permeability and provided with an interval-automatic water spraying device, performing cuttage on half-lignified scions at the temperature not lower than 8 DEG C, performing moisture regulation until rooting, transplanting in nutrition cups for arranged cultivation when 80% of the scions take roots and bud, well performing weeding, fertilizer-water management and disease-insect prevention, and cultivating to obtain Machilus pauhoi nursery stock, wherein moisture regulation includes: spraying water again before water films on the front of leaves disappear completely so as to keep the front of the leaves basically wet. By the method, cultivation cycle is short, outplanting only needs five to nine months, instable survival rate caused by weather differences can be solved, cultivation season is prolonged, problems such as seed source limitation can be solved, cultivation cost is lowered, and good economical, social and ecological benefits are achieved.

The invention discloses a method for tissue culture and rapid propagation of rosaceae Rosa deciduous erect shrub Rosa rugosa Thunb. belonging to the technical field of horticultural plant seedling propagation. The method is used for carrying out isolated culture on edible Rosa rugosa Thunb. caulicles to build a tissue culture and rapid propagation system of Rosa rugosa Thunb. and comprises four stages, namely selection and sterilization, primary culture, multiplication culture and rooting culture of an explant. The method disclosed by the invention is simple and has the advantages of high reproduction rate, short culture period and high reproduction coefficient, has relatively strong practicability in the actual production application, solves the technical problems of low reproduction rate and long reproduction period of a Rosa rugosa Thunb. seedling, and is suitable for commercial production of Rosa rugosa Thunb. seedlings so as to meet the urgent demand of a market.

The invention discloses an improved method for great castanopsis hystrix tree high-position grafting. According to the method, the scion grafting is carried out from middle and late January to late February every year when the average daily temperature is raised to 15+ / -3 DEG C, good-material castanopsis hystrix lignification branches are selected, wedge-shaped scions with 1 to 2 axillary buds are cut and taken, the scions are inserted into a cut of a stock so that at least one side of a forming layer of the stock and the scions is sufficiently anastomotic, in addition, the sealing binding is carried out on grafting parts, and finally, the high-position grafting process of great castanopsis hystrix trees is completed after the post-grafting management. The method provided by the invention is used for grafting the castanopsis hystrix and has the advantages that the culture period is short, the forming speed is high, the operation is simple, the cost is low, and the grafting survival rate is higher than 90 percent.

The invention provides a pure white new hypsizigus marmoreus bacterial strain Finc-W-62, and the preservation number of the bacterial strain is CCTCC NO: M 2012375. The culture and cultivation periods of Finc-W-62 are short, the production cost is low, pileus is not easy to open, tumor stropharia rugosannulata ratio is low, the appearance quality is improved and sporophore shelf life is long.

The invention discloses a brown hypsizygus marmoreus strain with high yield of glucan as well as a cultivation method and an application of the brown hypsizygus marmoreus strain. The glucan content of fruiting bodies of the brown hypsizygus marmoreus strain Finc-B-7 with high yield of glucan is 5%-10% on dry basis, the culture and cultivation period of the fruiting bodies is short, the per unit area yield is high, the production efficiency is improved greatly, and the production cost is reduced; the appearance uniformity of single strains as well as single fruiting bodies in single strain is high, and mechanized and automated operation is facilitated; pileus patterns are quite clear, the appearance is attractive, the commodity traits are good, the refreshing time is long, storage is facilitated, the overall characteristics of the strain are excellent, more strains with excellent characteristics can be cultured with the strain as an important parent, and the strain is quite suitable for large-area popularization for planting and has broad market prospect.

The invention relates to a new microbiological species, the phorozoon is Paecilomyces suffultus (Petch) Samson. The method consists of inoculating the said germ to parasite body or subculture medium, cultivating them in a proper condition to obtain a yellow-while fruiting body of Cordyceps. The said germ has advantages that it is suitable for artificial culture in a large number, the effective composition content is high, the adaptive capacity for environment is strong, the production velocity of bacterium is rapid, the stress is strong, the yield rate is high, the cultivated cycle is short and the germ can not degenerate.

The invention discloses a Tabebuia chrysantha root cutting reproduction method. According to the method, roots of Tabebuia chrysantha are used as as reproduction materials and the method comprises the steps of selection and treatment, preparation of cutting medium, planting, management and the like. Compared with the existing method, the Tabebuia chrysantha root cutting reproduction method is not influenced by seasons, the reproduction materials are easy to obtain, the operation is simple and easy to perform, the cultivation period is shorter, the transplanting survival rate is high and the economic benefits are remarkable. By using the Tabebuia chrysantha root cutting reproduction method, the quick cultivation of new Tabebuia chrysantha plants is facilitated, the market demand is satisfied and the significance to the popularization of the excellent landscaping tree species is great.

The invention discloses an agrocybe aegerita culture medium and a preparation method thereof. The solid part of the culture medium is prepared from the following raw materials in percentage by weight: 30-50% of miscellaneous tree dust, 20-30% of cottonseed hull, 15-25% of wheat bran, 5% of peanut cake, 5% of corn cob, 2-3% of quicklime, 1% of gypsum and 1% of saccharide. The preparation method comprises the following steps: (1) material pulverization; (2) material mixing; (3) stirring with water; and (4) packaging and sterilization. The agrocybe aegerita culture medium has the advantages of low cost and simple preparation process, and is suitable for growth of agrocybe aegerita. When being used for culturing the agrocybe aegerita, the culture medium has the advantages of high growth speed, short culture period and high yield.

The invention provides a new euonymus hamiltonianus species grafting seedling culture method, and relates to a technical method of rapidly and efficiently propagating new seedling species. The new euonymus hamiltonianus species grafting seedling culture method mainly resolves the problems that new euonymus hamiltonianus species have few sowing and propagation resources, grow slowly and have high culturing cost; a cutting propagation coefficient is low, and the period is long; grafting propagation scions lose water easily and have the small combination area, out-of-season grafting and storage are difficult, and the rate of survival is low when direct upper top-grafting is conducted on the scions. The method comprises the steps that (1) stocks are selected and processed; (2) the scions are selected; (4) double tongue grafting is conducted; (5) the scions are wax-sealed; (6) storage in sand, moisture preserving and heeling in temporary planting are conducted. The method uses the scion wax-sealing technique, double tongue grafting technique, the storage in sand, moisture preserving and heeling in temporary planting technique and the like, has the advantages of being short in culture period, high in propagating coefficient, low in labor cost and the like, and can be used for grafting propagation of different species of euonymus hamiltonianus.