Brown hypsizygus marmoreus strain with high yield of glucan as well as cultivation method and application of brown hypsizygus marmoreus strain

A technology of glucan and shiitake mushroom, applied in mushroom cultivation, microorganism-based methods, applications, etc., can solve problems such as low glucan content

Inactive Publication Date: 2017-05-17
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to disclose a high-yielding dextran-brown mushroom strain and its cultivation method and application, so as to overcome the defect of low glucan content existing in the prior art

Method used

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  • Brown hypsizygus marmoreus strain with high yield of glucan as well as cultivation method and application of brown hypsizygus marmoreus strain
  • Brown hypsizygus marmoreus strain with high yield of glucan as well as cultivation method and application of brown hypsizygus marmoreus strain
  • Brown hypsizygus marmoreus strain with high yield of glucan as well as cultivation method and application of brown hypsizygus marmoreus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 Polyspore selfing obtains Finc-B-7

[0104] The strain Finc-B-7 was obtained by polyspore self-breeding method.

[0105] Specific steps are as follows:

[0106] 1) Parental strain Finc-B-3;

[0107] 2) Prepare mother seed medium, original seed medium and cultivation medium:

[0108] Mother seed medium (PDA medium): 200g of potatoes, 20g of glucose, 20g of agar, and 1L of water are used for each 1L of medium.

[0109] Original seed culture medium: by weight percentage, comprising 40% original seed culture material and 60% water, described original seed culture material comprises by weight percentage:

[0110] Wood chips 65%

[0111] Rice Bran 18%

[0112] Bran 10%

[0113] Cornmeal 7%

[0114] Cultivation medium: by weight percentage, comprising 34% cultivar culture material and 66% water, said cultivar culture material comprises:

[0115]

[0116] 3) Isolation of basidiospores: place the cap of the parent bacterial strain Finc-B-3 fruiting body in a...

Embodiment 2

[0121] Embodiment 2 Antagonism experiment

[0122] Finc-B-7 of the present invention and parent strain Finc-B-3 are test materials, and the culture medium used is PDA medium.

[0123] Described PDA culture medium is with embodiment 1. Finc-B-7 and the parental strain Finc-B-3 were inoculated and cultured on the plate medium respectively, the inoculation points were 2.5cm apart, and cultured at 23°C for 20 days. Please refer to Table 1 for the results of this experiment.

[0124] Table 1 Antagonism test among different strains of P.

[0125] Variety Finc-B-3 Finc-B-7 antagonistic

[0126] Such as figure 1 , figure 2 As shown, there is an obvious antagonistic line between the junctions of the confrontation culture colonies, indicating that Finc-B-7 is a different strain of P. argentina from the parental strain Finc-B-3.

Embodiment 3

[0127] Embodiment 3 Cultivation experiment one

[0128] Finc-B-7 and the parental strain Finc-B-3 were cultured and cultivated respectively, and 4 bacterial ages were set from 65 days to 80 days, and every increase of 5 days was a bacterial age, and each bacterial age was 96 bottles, and then the The results of small batch cultivation are summarized and compared, and the advantages of the strain Finc-B-7 of the present invention are summarized.

[0129] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.

[0130] Female seed medium (PDA medium): refer to Example 1.

[0131] Original seed culture medium: by weight percentage, comprising 40% original seed culture material and 60% water, described original seed culture material comprises by weight percentage:

[0132] Wood chips 65%

[0133] Rice Bran 18%

[0134] Bran 10%

[0135] Cornmeal 7%

[0136] Cultivation medium: by weight percentage, comprising 34% cultivar culture material and 66% water, said cultivar c...

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Abstract

The invention discloses a brown hypsizygus marmoreus strain with high yield of glucan as well as a cultivation method and an application of the brown hypsizygus marmoreus strain. The glucan content of fruiting bodies of the brown hypsizygus marmoreus strain Finc-B-7 with high yield of glucan is 5%-10% on dry basis, the culture and cultivation period of the fruiting bodies is short, the per unit area yield is high, the production efficiency is improved greatly, and the production cost is reduced; the appearance uniformity of single strains as well as single fruiting bodies in single strain is high, and mechanized and automated operation is facilitated; pileus patterns are quite clear, the appearance is attractive, the commodity traits are good, the refreshing time is long, storage is facilitated, the overall characteristics of the strain are excellent, more strains with excellent characteristics can be cultured with the strain as an important parent, and the strain is quite suitable for large-area popularization for planting and has broad market prospect.

Description

technical field [0001] The present invention relates to a kind of mushroom strain. Background technique [0002] Basidiomycetes, Chinese alias Hypsizygus marmoreus (Latin name Hypsizygus marmoreus), belongs to Basidiomycotina, Phytomycota, Agaricaceae, White Mushrooms, Liphyllum family, and Amygium genus, because it has a unique crab Because of its umami taste, it is also called crab-flavored mushroom. At present, there are two varieties of brown and pure white cultivated, which are very popular in the market. [0003] The initial artificial cultivation began in Japan around 1978. It is delicious, crisp and tender, and rich in nutrition. The product is also full of fragrance. True jiji mushroom is rich in vitamins and 17 kinds of amino acids. The β-1,3-D glucan extracted from the fruiting body has high anti-tumor activity. It is a low-calorie, low-fat health food and has a broad future market expectation. [0004] The β-glucans, long considered non-specific stimuli in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04A01H1/02C12R1/645
CPCA01H1/02A01G18/00C12N1/14C12N1/145C12R2001/645
Inventor 韩成玲吴惠敏张文宏
Owner SHANGHAI FINC BIO TECH INC
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