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High-yielding dextran-brown mushroom strain and its cultivation method and application

A technology of jiji mushroom and dextran, which is applied in mushroom cultivation, microbial-based methods, cultivation, etc., and can solve the problem of low glucan content

Inactive Publication Date: 2019-04-05
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to disclose a high-yielding dextran-brown mushroom strain and its cultivation method and application, so as to overcome the defect of low glucan content existing in the prior art

Method used

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  • High-yielding dextran-brown mushroom strain and its cultivation method and application
  • High-yielding dextran-brown mushroom strain and its cultivation method and application
  • High-yielding dextran-brown mushroom strain and its cultivation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 Multispore self-crossing to obtain Finc-B-7

[0104] Finc-B-7 was obtained by multi-spore self-breeding method.

[0105] Specific steps are as follows:

[0106] 1) parental strain Finc-B-3;

[0107] 2) Preparation of mother seed medium, original seed medium and cultivation medium:

[0108] Mother seed medium (PDA medium): 200 g of potato, 20 g of glucose, 20 g of agar, and 1 L of water are used for each 1 L of medium.

[0109] Stock medium: by weight percentage, including 40% stock medium and 60% water, the stock medium by weight includes:

[0110] Sawdust 65%

[0111] Rice bran 18%

[0112] Bran 10%

[0113] Corn flour 7%

[0114] Cultivation medium: by weight percentage, including 34% cultivar culture material and 66% water, and the cultivar culture material includes:

[0115]

[0116] 3) Isolation of basidiospores: the caps of the fruiting bodies of the parental strain Finc-B-3 were placed in a petri dish. Spread on PDA medium plates.

[0117]C...

Embodiment 2

[0121] Example 2 Antagonism experiment

[0122] The Finc-B-7 of the present invention and the parent strain Finc-B-3 are test materials, and the medium used is PDA medium.

[0123] The PDA culture medium is the same as in Example 1. Finc-B-7 and parental strain Finc-B-3 were respectively inoculated and cultured on the plate medium, the inoculation points were 2.5cm apart, and incubated at 23°C for 20 days at a constant temperature. Please refer to Table 1 for the test results.

[0124] Table 1 Antagonism test between different brown shimeji strains

[0125] Variety

[0126] like figure 1 , figure 2 As shown, there is an obvious antagonistic line between the junctions of the confrontation culture colonies, indicating that Finc-B-7 is a brown shimeji strain different from the parental strain Finc-B-3.

Embodiment 3

[0127] Embodiment 3 Cultivation experiment one

[0128] The Finc-B-7 and the parent strain Finc-B-3 were cultured and cultivated respectively, and 4 bacterial ages were set from 65 days to 80 days. The results of small batch cultivation are summarized and compared, and the advantages of the strain Finc-B-7 of the present invention are summarized.

[0129] Cultivation bottle volume: 850cc, bottle diameter 58mm.

[0130] Mother seed medium (PDA medium): refer to Example 1.

[0131] Stock medium: by weight percentage, including 40% stock medium and 60% water, the stock medium by weight includes:

[0132] Sawdust 65%

[0133] Rice bran 18%

[0134] Bran 10%

[0135] Corn flour 7%

[0136] Cultivation medium: by weight percentage, including 34% cultivar culture material and 66% water, and the cultivar culture material includes:

[0137]

[0138] Stock seed preparation: insert the parent seed of the brown shiitake mushroom strain Finc-B-7 into stock medium, 23° C., air rel...

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Abstract

The invention discloses a high-glucan-yielding brown fungus strain Finc-B-7 and its cultivation method and application. The content of dextran is 5-10%. The brown shimeji mushroom strain Finc-B-7 provided by the present invention has a glucan content calculated on a dry basis, and a glucan content in fruiting bodies of 5-10%. Improve production efficiency and reduce production cost; the appearance uniformity between individual plants and single root fruiting bodies in a single plant is high, which is conducive to mechanized and automated operations; Long, it is good for storage, and the strain has excellent comprehensive characteristics. It can be used as an important parent to breed more strains with excellent performance. It is very suitable for large-scale promotion and planting, and has a broad market prospect.

Description

technical field [0001] The present invention relates to a kind of shimeji mushroom strain. Background technique [0002] Banyu mushroom, Chinese alias Hypsizygus marmoreus (Latin name Hypsizygus marmoreus), belongs to Basidiomycota, Laminaria, Agaric, White Mushroom, Lily Fungus, Yu Mushroom, because it has a unique crab It is also called crab-flavored mushroom because of its umami taste. At present, two strains of brown and pure white are cultivated, which are very popular in the market. [0003] The first artificial cultivation began in Japan around 1978. It has delicious taste, crisp and tender taste, and rich nutrition. Its taste is fresher than that of oyster mushrooms, its meat is thicker than that of shiitake mushrooms, its quality is tougher than that of shiitake mushrooms, and its taste is very good. The product is also fragrant. Shiji mushroom is rich in vitamins and 17 kinds of amino acids. The β-1,3-D glucan extracted from the fruiting body has high anti-tumor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/00A01H1/02C12R1/645
CPCA01H1/02A01G18/00C12N1/14C12N1/145C12R2001/645
Inventor 韩成玲吴惠敏张文宏
Owner SHANGHAI FINC BIO TECH INC
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