Fermenting culture process of produicng Cordyceps extract

A technology of fermentation culture and cordycepin, applied in the direction of plant cells, fungi, etc., to achieve the effect of increasing yield, shortening culture period and reducing cost

Inactive Publication Date: 2002-02-13
DALIAN PRACTICAL BIOTECH
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  • Abstract
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Problems solved by technology

In the published literature and patents at home and abroad, there is no report on the development of Cordyceps militaris polysaccharides and cordycepin by submerged fermentation in an air-lift cell reactor

Method used

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  • Fermenting culture process of produicng Cordyceps extract
  • Fermenting culture process of produicng Cordyceps extract

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Embodiment Construction

[0029] A 2L airlift cell reactor (purchased from the Biochemical Center of the Chinese Academy of Sciences) was used as a seed tank, and the liquid seeds were cultured at 26°C±1 for 96 hours, at a temperature of 24°C, and scattered light. The liquid seed medium consists of: 200 grams of potatoes, 10 grams of glucose, KH 2 PO 4 2 g, MgSO 4 1 gram, 5 grams of peptone, 2 grams of yeast extract.

[0030] After 5 days of liquid seed cultivation, select seed liquid with clear culture color, uniform bacterial balls, and a diameter of about 3mm, and inoculate it into a 20L air-lift fermenter at a 10% inoculum size, and cultivate it under sterile conditions. The cultivation of fermentation culture The base composition is the same as that of the above-mentioned seed liquid culture medium, the culture temperature is 24° C., and the culture time is 5 days, and the ventilation rate is 200 ml / min.

[0031] After the above-mentioned fermentation culture, the culture product was dried belo...

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Abstract

The present invention provides one method of culturing Northern Cordyceps in a airlift bioreactor in large scale and separating and extracting Cordyceps extract. Seed for fermentation is cultured in MS culture medium and then transferred to 20 L bioreactor for large scale culture. The culture liquid in the bioreactor is then separated and purified to obtain product with Cordyceps extract content up to 46.25 % and purity of 99 %. The process of the present invention has low production cost and no environmental pollution.

Description

technical field [0001] The invention relates to a method for producing cordycepin through fermentation and cultivation. Specifically, the plant cell culture technology is used as a means to produce cordycepin through large-scale cultivation of Cordyceps militaris carried out in a cell bioreactor. The invention belongs to the field of biopharmaceuticals. Background technique [0002] Corduceps militaris L; Sp, PL is a solitary or several fungal subunit of Ascomycotina (Ascomycotina), Ergot (Clavicipitales), Ergotaceae (Cordycepps) from the head of the insect, sometimes from the nodes. , orange yellow, mostly unbranched, 2-5 cm high, rod-shaped head, 1-2 cm long, 0.3-0.5 cm thick. Therapeutic food is the same as Cordyceps sinensis. The asexual reproduction is Paecilmyces militarae. It has been proved by modern science that Cordyceps militaris not only has special nutritional value, but also has obvious medicinal value. Since ancient times, Cordyceps sinensis has been regar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N5/04
Inventor 贾景明张剑侠吴立军
Owner DALIAN PRACTICAL BIOTECH
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